P43418 (T2G1_HAEGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme HgAI
Short name=R.HgAI
EC=3.1.21.4

Alternative name(s):
Endonuclease HgAI
Type II restriction enzyme HgAI

Gene names

Name:

hgaIR

Organism

Haemophilus gallinarum

Taxonomic identifier

728 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Avibacterium

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequences GACGC and GCGTC and cleaves respectively 10 bases after G-1 and 10 bases before G'-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Type-2 restriction enzyme HgAI		PRO_0000077309

Sequences

	Sequence		Length	Mass (Da)
	P43418 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: B5E05259E3C710D2	FASTA	488	56,737
	10 20 30 40 50 60 MEKILMLNDD QIWIFKKHTN NIQLLIEVAL YLKSNKSSVS KKDKDAMYDI FSESELYNPR 70 80 90 100 110 120 ESLRDKPLDT INHKLDGLSY FMFGYSDRIN DENKFIFSPL GNLFLKYLHD KDKLSKIFSC 130 140 150 160 170 180 MLISMQFPHP YSKPSECFLL YPFRLIFKLL LDKRLQGRLY HYEVYKIIIH TISIDEAKYE 190 200 210 220 230 240 FLVKSILNSR KKSWNEKLNE LSEIQHKVVK SVYEWQYYIV PLLGSLHIFK INNGDIEQKL 250 260 270 280 290 300 YHPQKDGSKS PPTARKANNG YVEINDNLTN FIDKLLNKYS FLDTPILLSD SQRKSNDVTK 310 320 330 340 350 360 EIYSFYPELL LAEIGETISF ESHILNIPKL ITEYSKNPDN STSGKFEKIL EEAFNLFIDV 370 380 390 400 410 420 EAQWLAGAGR TDIECMYLPI NEKFSIEAKS TKNKLSMINS GRLKRHRTLI SANYTIVITP 430 440 450 460 470 480 RYVPSVRYDI EAQDIVLITA DTLAEYLYNN IISNNRDISY ADIQAIIVAN LGKDISTQIS NLTLSKFG

References

[1]	"Nucleotide sequence of the gene of HgaI restriction endonuclease." Sugisaki H. Bull. Inst. Chem. Res., Kyoto Univ. 71:338-342(1993) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCTC 3438.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17388 Genomic DNA. Translation: BAA04208.1.
PIR	C59240.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018573. Restrct_endonuc_II_AlwI. [Graphical view]
Pfam	PF09491. RE_AlwI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2G1_HAEGA

Accession

Primary (citable) accession number: P43418

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	July 11, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program