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P43418 (T2G1_AVIPA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme HgAI

Short name=R.HgAI
EC=3.1.21.4
Alternative name(s):
Endonuclease HgAI
Type II restriction enzyme HgAI
Gene names
Name:hgaIR
OrganismAvibacterium paragallinarum (Haemophilus gallinarum)
Taxonomic identifier728 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeAvibacterium

Protein attributes

Sequence length488 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequences GACGC and GCGTC and cleaves respectively 10 bases after G-1 and 10 bases before G'-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 488488Type-2 restriction enzyme HgAI
PRO_0000077309

Sequences

Sequence LengthMass (Da)Tools
P43418 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: B5E05259E3C710D2

FASTA48856,737
        10         20         30         40         50         60 
MEKILMLNDD QIWIFKKHTN NIQLLIEVAL YLKSNKSSVS KKDKDAMYDI FSESELYNPR 

        70         80         90        100        110        120 
ESLRDKPLDT INHKLDGLSY FMFGYSDRIN DENKFIFSPL GNLFLKYLHD KDKLSKIFSC 

       130        140        150        160        170        180 
MLISMQFPHP YSKPSECFLL YPFRLIFKLL LDKRLQGRLY HYEVYKIIIH TISIDEAKYE 

       190        200        210        220        230        240 
FLVKSILNSR KKSWNEKLNE LSEIQHKVVK SVYEWQYYIV PLLGSLHIFK INNGDIEQKL 

       250        260        270        280        290        300 
YHPQKDGSKS PPTARKANNG YVEINDNLTN FIDKLLNKYS FLDTPILLSD SQRKSNDVTK 

       310        320        330        340        350        360 
EIYSFYPELL LAEIGETISF ESHILNIPKL ITEYSKNPDN STSGKFEKIL EEAFNLFIDV 

       370        380        390        400        410        420 
EAQWLAGAGR TDIECMYLPI NEKFSIEAKS TKNKLSMINS GRLKRHRTLI SANYTIVITP 

       430        440        450        460        470        480 
RYVPSVRYDI EAQDIVLITA DTLAEYLYNN IISNNRDISY ADIQAIIVAN LGKDISTQIS 


NLTLSKFG 

« Hide

References

[1]"Nucleotide sequence of the gene of HgaI restriction endonuclease."
Sugisaki H.
Bull. Inst. Chem. Res., Kyoto Univ. 71:338-342(1993)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NCTC 3438.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D17388 Genomic DNA. Translation: BAA04208.1.
PIRC59240.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018573. Restrct_endonuc_II_AlwI.
[Graphical view]
PfamPF09491. RE_AlwI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2G1_AVIPA
AccessionPrimary (citable) accession number: P43418
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries