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P43418

- T2G1_AVIPA

UniProt

P43418 - T2G1_AVIPA

Protein

Type-2 restriction enzyme HgAI

Gene

hgaIR

Organism
Avibacterium paragallinarum (Haemophilus gallinarum)
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 43 (16 Apr 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequences GACGC and GCGTC and cleaves respectively 10 bases after G-1 and 10 bases before G'-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme HgAI (EC:3.1.21.4)
    Short name:
    R.HgAI
    Alternative name(s):
    Endonuclease HgAI
    Type II restriction enzyme HgAI
    Gene namesi
    Name:hgaIR
    OrganismiAvibacterium paragallinarum (Haemophilus gallinarum)
    Taxonomic identifieri728 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeAvibacterium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 488488Type-2 restriction enzyme HgAIPRO_0000077309Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR018573. Restrct_endonuc_II_AlwI.
    [Graphical view]
    PfamiPF09491. RE_AlwI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43418-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKILMLNDD QIWIFKKHTN NIQLLIEVAL YLKSNKSSVS KKDKDAMYDI    50
    FSESELYNPR ESLRDKPLDT INHKLDGLSY FMFGYSDRIN DENKFIFSPL 100
    GNLFLKYLHD KDKLSKIFSC MLISMQFPHP YSKPSECFLL YPFRLIFKLL 150
    LDKRLQGRLY HYEVYKIIIH TISIDEAKYE FLVKSILNSR KKSWNEKLNE 200
    LSEIQHKVVK SVYEWQYYIV PLLGSLHIFK INNGDIEQKL YHPQKDGSKS 250
    PPTARKANNG YVEINDNLTN FIDKLLNKYS FLDTPILLSD SQRKSNDVTK 300
    EIYSFYPELL LAEIGETISF ESHILNIPKL ITEYSKNPDN STSGKFEKIL 350
    EEAFNLFIDV EAQWLAGAGR TDIECMYLPI NEKFSIEAKS TKNKLSMINS 400
    GRLKRHRTLI SANYTIVITP RYVPSVRYDI EAQDIVLITA DTLAEYLYNN 450
    IISNNRDISY ADIQAIIVAN LGKDISTQIS NLTLSKFG 488
    Length:488
    Mass (Da):56,737
    Last modified:November 1, 1995 - v1
    Checksum:iB5E05259E3C710D2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17388 Genomic DNA. Translation: BAA04208.1.
    PIRiC59240.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17388 Genomic DNA. Translation: BAA04208.1 .
    PIRi C59240.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR018573. Restrct_endonuc_II_AlwI.
    [Graphical view ]
    Pfami PF09491. RE_AlwI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the gene of HgaI restriction endonuclease."
      Sugisaki H.
      Bull. Inst. Chem. Res., Kyoto Univ. 71:338-342(1993)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: NCTC 3438.

    Entry informationi

    Entry nameiT2G1_AVIPA
    AccessioniPrimary (citable) accession number: P43418
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: April 16, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3