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P43406 (ITAV_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Integrin alpha-V
Alternative name(s):
Vitronectin receptor subunit alpha
CD_antigen=CD51
Gene names
Name:Itgav
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1044 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. Alpha-V integrins may play a role in embryo implantation, angiogenesis and wound healing. Mice expressing a null mutation of the alpha-V subunit gene survive until late in embryonic development and occasionally even to birth. They demonstrate cleft palate, and defective development of CNS and gastrointestinal blood vessels.

Subunit structure

Interacts with RAB25. Interacts with CIB1 By similarity. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8.

Subcellular location

Membrane; Single-pass type I membrane protein.

Sequence similarities

Belongs to the integrin alpha chain family.

Contains 7 FG-GAP repeats.

Ontologies

Keywords
   Biological processAngiogenesis
Cell adhesion
Differentiation
   Cellular componentMembrane
   DomainRepeat
Signal
Transmembrane
Transmembrane helix
   LigandCalcium
Metal-binding
   Molecular functionDevelopmental protein
Integrin
Receptor
   PTMCleavage on pair of basic residues
Disulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processERK1 and ERK2 cascade

Inferred from direct assay PubMed 16014375. Source: BHF-UCL

T cell activation

Traceable author statement PubMed 12099514. Source: BHF-UCL

angiogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

apoptotic cell clearance

Inferred from mutant phenotype PubMed 15647754. Source: MGI

blood vessel development

Inferred from mutant phenotype PubMed 12370313. Source: MGI

cell adhesion

Inferred from sequence or structural similarity. Source: BHF-UCL

cell-matrix adhesion

Traceable author statement PubMed 10965141. Source: BHF-UCL

detection of molecule of bacterial origin

Traceable author statement PubMed 10570297. Source: BHF-UCL

endothelial cell migration

Traceable author statement PubMed 12099514. Source: BHF-UCL

extrinsic apoptotic signaling pathway in absence of ligand

Inferred from direct assay PubMed 15591537. Source: BHF-UCL

fertilization

Non-traceable author statement PubMed 20151413. Source: BHF-UCL

focal adhesion assembly

Non-traceable author statement PubMed 12878490. Source: BHF-UCL

gonad morphogenesis

Non-traceable author statement PubMed 16571912. Source: BHF-UCL

inflammatory response

Traceable author statement PubMed 20826760. Source: BHF-UCL

integrin-mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of entry of bacterium into host cell

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of extrinsic apoptotic signaling pathway

Inferred from electronic annotation. Source: Ensembl

negative regulation of lipid storage

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of lipid transport

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of lipoprotein metabolic process

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of low-density lipoprotein particle receptor biosynthetic process

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of macrophage derived foam cell differentiation

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of cell adhesion

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of cell migration

Inferred from mutant phenotype PubMed 20675382. Source: BHF-UCL

positive regulation of cell proliferation

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of osteoblast proliferation

Inferred from mutant phenotype PubMed 20675382. Source: BHF-UCL

regulation of apoptotic cell clearance

Inferred from direct assay PubMed 20826760. Source: BHF-UCL

regulation of bone resorption

Traceable author statement PubMed 16014375. Source: BHF-UCL

regulation of phagocytosis

Inferred from sequence or structural similarity. Source: BHF-UCL

release of sequestered calcium ion into cytosol

Traceable author statement PubMed 16014375. Source: BHF-UCL

response to nitric oxide

Traceable author statement PubMed 21307347. Source: BHF-UCL

trophoblast giant cell differentiation

Traceable author statement PubMed 19228596. Source: BHF-UCL

   Cellular_componentalphav-beta3 integrin complex

Traceable author statement PubMed 10965141. Source: BHF-UCL

alphav-beta3 integrin-IGF-1-IGF1R complex

Inferred from electronic annotation. Source: Ensembl

cell surface

Inferred from sequence or structural similarity. Source: BHF-UCL

external side of plasma membrane

Inferred from direct assay PubMed 8557754PubMed 9553049. Source: MGI

integrin complex

Inferred from sequence or structural similarity. Source: BHF-UCL

membrane

Inferred from direct assay PubMed 20151413. Source: BHF-UCL

plasma membrane

Inferred from direct assay PubMed 14566019. Source: MGI

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein heterodimerization activity

Traceable author statement PubMed 20958318. Source: BHF-UCL

transforming growth factor beta binding

Inferred from sequence or structural similarity. Source: BHF-UCL

voltage-gated calcium channel activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 By similarity
Chain31 – 10441014Integrin alpha-V
PRO_0000016304
Chain31 – 885855Integrin alpha-V heavy chain By similarity
PRO_0000016305
Chain887 – 1044158Integrin alpha-V light chain By similarity
PRO_0000016306

Regions

Topological domain31 – 988958Extracellular Potential
Transmembrane989 – 101224Helical; Potential
Topological domain1013 – 104432Cytoplasmic Potential
Repeat32 – 9867FG-GAP 1
Repeat109 – 17062FG-GAP 2
Repeat173 – 22553FG-GAP 3
Repeat237 – 29559FG-GAP 4
Repeat296 – 35762FG-GAP 5
Repeat358 – 41558FG-GAP 6
Repeat419 – 48264FG-GAP 7
Calcium binding260 – 2689 Potential
Calcium binding314 – 3229 Potential
Calcium binding379 – 3879 Potential
Calcium binding443 – 4519 Potential
Motif1015 – 10195GFFKR motif

Amino acid modifications

Glycosylation741N-linked (GlcNAc...) Ref.3 Ref.4
Glycosylation2901N-linked (GlcNAc...) Potential
Glycosylation2961N-linked (GlcNAc...) Potential
Glycosylation6151N-linked (GlcNAc...) Ref.3 Ref.4
Glycosylation7041N-linked (GlcNAc...) Potential
Glycosylation8351N-linked (GlcNAc...) Potential
Glycosylation8511N-linked (GlcNAc...) Potential
Glycosylation8691N-linked (GlcNAc...) Ref.3 Ref.4
Glycosylation9391N-linked (GlcNAc...); atypical Ref.3
Glycosylation9411N-linked (GlcNAc...) Ref.3
Glycosylation9691N-linked (GlcNAc...) Potential
Glycosylation9761N-linked (GlcNAc...) Potential
Disulfide bond89 ↔ 97 By similarity
Disulfide bond138 ↔ 158 By similarity
Disulfide bond172 ↔ 185 By similarity
Disulfide bond491 ↔ 502 By similarity
Disulfide bond508 ↔ 565 By similarity
Disulfide bond626 ↔ 632 By similarity
Disulfide bond698 ↔ 711 By similarity
Disulfide bond852 ↔ 900Interchain (between heavy and light chains) By similarity
Disulfide bond905 ↔ 910 By similarity

Experimental info

Sequence conflict526 – 5272EL → DV in AAC52497. Ref.1
Sequence conflict768 – 7692VL → EK in AAC52497. Ref.1
Sequence conflict8721A → G in AAC52497. Ref.1
Sequence conflict8801N → S in AAC52497. Ref.1
Sequence conflict8871D → G in AAC52497. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P43406 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 417FD73D776B5918

FASTA1,044115,360
        10         20         30         40         50         60 
MAAPGRLLLR PRPGGLLLLL PGLLLPLADA FNLDVESPAE YAGPEGSYFG FAVDFFEPST 

        70         80         90        100        110        120 
SSRMFLLVGA PKANTTQPGI VEGGQVLKCE CSSSRRCQPI EFDSTGNRDY AKDDPLEFKS 

       130        140        150        160        170        180 
HQWFGASVRS KQDKILACAP LYHWRTEMKQ EREPVGTCFL QDGTKTVEYA PCRSKNIDAD 

       190        200        210        220        230        240 
GQGFCQGGFS IDFTKADRVL LGGPGSFYWQ GQLISDQVAE IISKYDPNVY SIKYNNQLAT 

       250        260        270        280        290        300 
RTAQAIFDDS YLGYSVAVGD FNGDGIEDFV SGVPRAARTL GMVYIYDGKN MSSLHNFTGE 

       310        320        330        340        350        360 
QMAAYFGFSV AATDINGDDY ADVFIGAPLF MDRGSDGKLQ EVGQVSVSLQ RAVGDFQTTK 

       370        380        390        400        410        420 
LNGFEVFARF GSAIAPLGDL DQDGFNDIAI AAPYGGEDKK GLVYIFNGRS TGLNSVPSQI 

       430        440        450        460        470        480 
LEGQWAAQSM PPSFGYSMKG ATDVDRNGYP DLVVGAFGVD RAVLYRARPV VTVNAGLEVY 

       490        500        510        520        530        540 
PSILNQDNKI CPLPGTALKV SCFNVRFCLK ADGKGTLPRK LHFQVELLLD KLKQKGAIRR 

       550        560        570        580        590        600 
ALFLHNRSPV HSKTMTVFRG GQMQCEELVA YLRDESEFRD KLTPITIFME YRLDQRTAAD 

       610        620        630        640        650        660 
ATGLQPILNQ FTPANVSRQA HILLDCGEDN VCKPKLEVSV NSDQKKIYIG DDNPLTLTVK 

       670        680        690        700        710        720 
AQNQGEGAYE AELIVSIPPQ ADFIGVVRNN EALARLSCAF KTENQTRQVV CDLGNPMKAG 

       730        740        750        760        770        780 
TQLLAGLRFS VHQQSEMDTS VKFDLKIQSS NSFDNVSPVV SYKVDLAVLA AVEIRGVSSP 

       790        800        810        820        830        840 
DHIFLPIPNW EYKENPETEE DVGPIVQHIY ELRNNGPSSF SKAILNLQWP YKYNNNTLLY 

       850        860        870        880        890        900 
ILHYDIDGPM NCTADTEINP LRIKTPEKND TAAAGQGERN HLITKRDLTL REGDVHTLGC 

       910        920        930        940        950        960 
GIAKCLQITC QVGRLDRGKS AILYVKSLLW TETFMNKENQ NHSYSLKSSA SFNIIEFPYK 

       970        980        990       1000       1010       1020 
NLPIEDLFNS TLVTTNITWG IQPAPMPVPV WVIILAVLAG LLLLAVLVFV MYRMGFFKRV 

      1030       1040 
RPPQEEQERE QLQPHENGEG NSET 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of mouse integrin alphaV cDNA and role of the alphaV-related matrix receptors in metanephric development."
Wada J., Kumar A., Liu Z., Ruoslahti E., Reichardt L., Marvaldi J., Kanwar Y.S.
J. Cell Biol. 132:1161-1176(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: CD-1.
Tissue: Kidney.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74; ASN-615; ASN-869; ASN-939 AND ASN-941.
Tissue: Myoblast.
[4]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74; ASN-615 AND ASN-869.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U14135 mRNA. Translation: AAC52497.1.
AL772301 Genomic DNA. Translation: CAM13680.1.
CCDSCCDS16181.1.
PIRT10050.
RefSeqNP_032428.2. NM_008402.3.
UniGeneMm.227.

3D structure databases

ProteinModelPortalP43406.
SMRP43406. Positions 31-1027.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP43406. 4 interactions.
MINTMINT-4098999.

Chemistry

BindingDBP43406.

PTM databases

PhosphoSiteP43406.

Proteomic databases

MaxQBP43406.
PaxDbP43406.
PRIDEP43406.

Protocols and materials databases

DNASU16410.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028499; ENSMUSP00000028499; ENSMUSG00000027087.
GeneID16410.
KEGGmmu:16410.
UCSCuc008kid.1. mouse.

Organism-specific databases

CTD3685.
MGIMGI:96608. Itgav.

Phylogenomic databases

eggNOGNOG26407.
GeneTreeENSGT00750000117267.
HOGENOMHOG000231603.
HOVERGENHBG006186.
InParanoidA2AKI6.
KOK06487.
OMADKNGYPD.
OrthoDBEOG7R830R.
TreeFamTF105391.

Gene expression databases

ArrayExpressP43406.
BgeeP43406.
CleanExMM_ITGAV.
GenevestigatorP43406.

Family and domain databases

InterProIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
[Graphical view]
PfamPF01839. FG-GAP. 2 hits.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSPR01185. INTEGRINA.
SMARTSM00191. Int_alpha. 5 hits.
[Graphical view]
PROSITEPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSITGAV. mouse.
NextBio289589.
PROP43406.
SOURCESearch...

Entry information

Entry nameITAV_MOUSE
AccessionPrimary (citable) accession number: P43406
Secondary accession number(s): A2AKI6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot