P43406 (ITAV_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Integrin alpha-V

Alternative name(s):
Vitronectin receptor subunit alpha
CD_antigen=CD51

Cleaved into the following 2 chains:

1. Integrin alpha-V heavy chain
2. Integrin alpha-V light chain

Gene names

Name:

Itgav

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1044 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. Alpha-V integrins may play a role in embryo implantation, angiogenesis and wound healing. Mice expressing a null mutation of the alpha-V subunit gene survive until late in embryonic development and occasionally even to birth. They demonstrate cleft palate, and defective development of CNS and gastrointestinal blood vessels.
Subunit structure	Interacts with RAB25 By similarity. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.

Ontologies

Keywords
Biological process	Angiogenesis Cell adhesion Differentiation
Cellular component	Membrane
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium Metal-binding
Molecular function	Developmental protein Integrin Receptor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ERK1 and ERK2 cascade Inferred from direct assay PubMed 16014375. Source: BHF-UCL T cell activation Traceable author statement PubMed 12099514. Source: BHF-UCL angiogenesis Inferred from electronic annotation. Source: UniProtKB-KW apoptotic cell clearance Inferred from mutant phenotype PubMed 15647754. Source: MGI blood vessel development Inferred from mutant phenotype PubMed 12370313. Source: MGI detection of molecule of bacterial origin Traceable author statement PubMed 10570297. Source: BHF-UCL endothelial cell migration Traceable author statement PubMed 12099514. Source: BHF-UCL fertilization Non-traceable author statement PubMed 20151413. Source: BHF-UCL focal adhesion assembly Non-traceable author statement PubMed 12878490. Source: BHF-UCL gonad morphogenesis Non-traceable author statement PubMed 16571912. Source: BHF-UCL inflammatory response Traceable author statement PubMed 20826760. Source: BHF-UCL integrin-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of apoptotic process Inferred from direct assay PubMed 15591537PubMed 16888388. Source: BHF-UCL negative regulation of entry of bacterium into host cell Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of lipid storage Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of lipid transport Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of lipoprotein metabolic process Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of low-density lipoprotein particle receptor biosynthetic process Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of macrophage derived foam cell differentiation Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of cell adhesion Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of cell migration Inferred from mutant phenotype PubMed 20675382. Source: BHF-UCL positive regulation of osteoblast proliferation Inferred from mutant phenotype PubMed 20675382. Source: BHF-UCL regulation of apoptotic cell clearance Inferred from direct assay PubMed 20826760. Source: BHF-UCL regulation of bone resorption Traceable author statement PubMed 16014375. Source: BHF-UCL release of sequestered calcium ion into cytosol Traceable author statement PubMed 16014375. Source: BHF-UCL response to nitric oxide Traceable author statement PubMed 21307347. Source: BHF-UCL trophoblast giant cell differentiation Traceable author statement PubMed 19228596. Source: BHF-UCL
Cellular_component	alphav-beta3 integrin complex Traceable author statement PubMed 10965141. Source: BHF-UCL alphav-beta3 integrin-IGF-1-IGF1R complex Inferred from electronic annotation. Source: Compara external side of plasma membrane Inferred from direct assay PubMed 8557754. Source: MGI nucleus Inferred from sequence orthology PubMed 22027834. Source: MGI
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein heterodimerization activity Traceable author statement PubMed 20958318. Source: BHF-UCL transforming growth factor beta binding Inferred from sequence or structural similarity. Source: BHF-UCL voltage-gated calcium channel activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	By similarity
	Chain	31 – 1044	1014	Integrin alpha-V		PRO_0000016304
	Chain	31 – 885	855	Integrin alpha-V heavy chain By similarity		PRO_0000016305
	Chain	887 – 1044	158	Integrin alpha-V light chain By similarity		PRO_0000016306
Regions
	Topological domain	31 – 988	958	Extracellular Potential
	Transmembrane	989 – 1012	24	Helical; Potential
	Topological domain	1013 – 1044	32	Cytoplasmic Potential
	Repeat	32 – 98	67	FG-GAP 1
	Repeat	109 – 170	62	FG-GAP 2
	Repeat	173 – 225	53	FG-GAP 3
	Repeat	237 – 295	59	FG-GAP 4
	Repeat	296 – 357	62	FG-GAP 5
	Repeat	358 – 415	58	FG-GAP 6
	Repeat	419 – 482	64	FG-GAP 7
	Calcium binding	260 – 268	9	Potential
	Calcium binding	314 – 322	9	Potential
	Calcium binding	379 – 387	9	Potential
	Calcium binding	443 – 451	9	Potential
	Motif	1015 – 1019	5	GFFKR motif
Amino acid modifications
	Glycosylation	74	1	N-linked (GlcNAc...) Ref.3 Ref.4
	Glycosylation	290	1	N-linked (GlcNAc...) Potential
	Glycosylation	296	1	N-linked (GlcNAc...) Potential
	Glycosylation	615	1	N-linked (GlcNAc...) Ref.3 Ref.4
	Glycosylation	704	1	N-linked (GlcNAc...) Potential
	Glycosylation	835	1	N-linked (GlcNAc...) Potential
	Glycosylation	851	1	N-linked (GlcNAc...) Potential
	Glycosylation	869	1	N-linked (GlcNAc...) Ref.3 Ref.4
	Glycosylation	939	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	941	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	969	1	N-linked (GlcNAc...) Potential
	Glycosylation	976	1	N-linked (GlcNAc...) Potential
	Disulfide bond	89 ↔ 97		By similarity
	Disulfide bond	138 ↔ 158		By similarity
	Disulfide bond	172 ↔ 185		By similarity
	Disulfide bond	491 ↔ 502		By similarity
	Disulfide bond	508 ↔ 565		By similarity
	Disulfide bond	626 ↔ 632		By similarity
	Disulfide bond	698 ↔ 711		By similarity
	Disulfide bond	852 ↔ 900		Interchain (between heavy and light chains) By similarity
	Disulfide bond	905 ↔ 910		By similarity
Experimental info
	Sequence conflict	526 – 527	2	EL → DV in AAC52497. Ref.1
	Sequence conflict	768 – 769	2	VL → EK in AAC52497. Ref.1
	Sequence conflict	872	1	A → G in AAC52497. Ref.1
	Sequence conflict	880	1	N → S in AAC52497. Ref.1
	Sequence conflict	887	1	D → G in AAC52497. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P43406 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 417FD73D776B5918	FASTA	1,044	115,360
	10 20 30 40 50 60 MAAPGRLLLR PRPGGLLLLL PGLLLPLADA FNLDVESPAE YAGPEGSYFG FAVDFFEPST 70 80 90 100 110 120 SSRMFLLVGA PKANTTQPGI VEGGQVLKCE CSSSRRCQPI EFDSTGNRDY AKDDPLEFKS 130 140 150 160 170 180 HQWFGASVRS KQDKILACAP LYHWRTEMKQ EREPVGTCFL QDGTKTVEYA PCRSKNIDAD 190 200 210 220 230 240 GQGFCQGGFS IDFTKADRVL LGGPGSFYWQ GQLISDQVAE IISKYDPNVY SIKYNNQLAT 250 260 270 280 290 300 RTAQAIFDDS YLGYSVAVGD FNGDGIEDFV SGVPRAARTL GMVYIYDGKN MSSLHNFTGE 310 320 330 340 350 360 QMAAYFGFSV AATDINGDDY ADVFIGAPLF MDRGSDGKLQ EVGQVSVSLQ RAVGDFQTTK 370 380 390 400 410 420 LNGFEVFARF GSAIAPLGDL DQDGFNDIAI AAPYGGEDKK GLVYIFNGRS TGLNSVPSQI 430 440 450 460 470 480 LEGQWAAQSM PPSFGYSMKG ATDVDRNGYP DLVVGAFGVD RAVLYRARPV VTVNAGLEVY 490 500 510 520 530 540 PSILNQDNKI CPLPGTALKV SCFNVRFCLK ADGKGTLPRK LHFQVELLLD KLKQKGAIRR 550 560 570 580 590 600 ALFLHNRSPV HSKTMTVFRG GQMQCEELVA YLRDESEFRD KLTPITIFME YRLDQRTAAD 610 620 630 640 650 660 ATGLQPILNQ FTPANVSRQA HILLDCGEDN VCKPKLEVSV NSDQKKIYIG DDNPLTLTVK 670 680 690 700 710 720 AQNQGEGAYE AELIVSIPPQ ADFIGVVRNN EALARLSCAF KTENQTRQVV CDLGNPMKAG 730 740 750 760 770 780 TQLLAGLRFS VHQQSEMDTS VKFDLKIQSS NSFDNVSPVV SYKVDLAVLA AVEIRGVSSP 790 800 810 820 830 840 DHIFLPIPNW EYKENPETEE DVGPIVQHIY ELRNNGPSSF SKAILNLQWP YKYNNNTLLY 850 860 870 880 890 900 ILHYDIDGPM NCTADTEINP LRIKTPEKND TAAAGQGERN HLITKRDLTL REGDVHTLGC 910 920 930 940 950 960 GIAKCLQITC QVGRLDRGKS AILYVKSLLW TETFMNKENQ NHSYSLKSSA SFNIIEFPYK 970 980 990 1000 1010 1020 NLPIEDLFNS TLVTTNITWG IQPAPMPVPV WVIILAVLAG LLLLAVLVFV MYRMGFFKRV 1030 1040 RPPQEEQERE QLQPHENGEG NSET

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of mouse integrin alphaV cDNA and role of the alphaV-related matrix receptors in metanephric development." Wada J., Kumar A., Liu Z., Ruoslahti E., Reichardt L., Marvaldi J., Kanwar Y.S. J. Cell Biol. 132:1161-1176(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CD-1. Tissue: Kidney.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation." Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B. Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74; ASN-615; ASN-869; ASN-939 AND ASN-941, MASS SPECTROMETRY. Tissue: Myoblast.
[4]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74; ASN-615 AND ASN-869, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14135 mRNA. Translation: AAC52497.1. AL772301 Genomic DNA. Translation: CAM13680.1.
IPI	IPI00120245.
PIR	T10050.
RefSeq	NP_032428.2. NM_008402.2.
UniGene	Mm.227.
3D structure databases
ProteinModelPortal	P43406.
SMR	P43406. Positions 31-1030.
ModBase	Search...
PTM databases
PhosphoSite	P43406.
Proteomic databases
PaxDb	P43406.
PRIDE	P43406.
Protocols and materials databases
DNASU	16410.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000028499; ENSMUSP00000028499; ENSMUSG00000027087.
GeneID	16410.
KEGG	mmu:16410.
Organism-specific databases
CTD	3685.
MGI	MGI:96608. Itgav.
Phylogenomic databases
eggNOG	NOG26407.
GeneTree	ENSGT00700000104007.
HOGENOM	HOG000231603.
HOVERGEN	HBG006186.
InParanoid	A2AKI6.
KO	K06487.
OMA	SRMFLLV.
OrthoDB	EOG46DM20.
Gene expression databases
ArrayExpress	P43406.
Bgee	P43406.
CleanEx	MM_ITGAV.
Genevestigator	P43406.
GermOnline	ENSMUSG00000027087. Mus musculus.
Family and domain databases
InterPro	IPR013517. FG-GAP. IPR013519. Int_alpha_beta-p. IPR000413. Integrin_alpha. IPR013649. Integrin_alpha-2. IPR018184. Integrin_alpha_C_CS. [Graphical view]
Pfam	PF01839. FG-GAP. 2 hits. PF00357. Integrin_alpha. 1 hit. PF08441. Integrin_alpha2. 1 hit. [Graphical view]
PRINTS	PR01185. INTEGRINA.
SMART	SM00191. Int_alpha. 5 hits. [Graphical view]
PROSITE	PS51470. FG_GAP. 7 hits. PS00242. INTEGRIN_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P43406.
ChEMBL	CHEMBL5565.
ChiTaRS	ITGAV. mouse.
NextBio	289589.
SOURCE	Search...

Entry information

Entry name

ITAV_MOUSE

Accession

Primary (citable) accession number: P43406
Secondary accession number(s): A2AKI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 120 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program