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Protein

Integrin alpha-V

Gene

Itgav

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. Alpha-V integrins may play a role in embryo implantation, angiogenesis and wound healing. Mice expressing a null mutation of the alpha-V subunit gene survive until late in embryonic development and occasionally even to birth. They demonstrate cleft palate, and defective development of CNS and gastrointestinal blood vessels.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi260 – 2689Sequence Analysis
Calcium bindingi314 – 3229Sequence Analysis
Calcium bindingi379 – 3879Sequence Analysis
Calcium bindingi443 – 4519Sequence Analysis

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: UniProtKB-KW
  • apolipoprotein A-I-mediated signaling pathway Source: MGI
  • apoptotic cell clearance Source: MGI
  • blood vessel development Source: MGI
  • calcium ion transmembrane transport Source: MGI
  • cell adhesion Source: BHF-UCL
  • cell growth Source: MGI
  • cell-matrix adhesion Source: BHF-UCL
  • cell migration Source: MGI
  • cell-substrate adhesion Source: MGI
  • detection of molecule of bacterial origin Source: BHF-UCL
  • endodermal cell differentiation Source: MGI
  • endothelial cell migration Source: BHF-UCL
  • ERK1 and ERK2 cascade Source: BHF-UCL
  • extrinsic apoptotic signaling pathway in absence of ligand Source: BHF-UCL
  • fertilization Source: BHF-UCL
  • focal adhesion assembly Source: BHF-UCL
  • gonad morphogenesis Source: BHF-UCL
  • heterotypic cell-cell adhesion Source: MGI
  • inflammatory response Source: BHF-UCL
  • integrin-mediated signaling pathway Source: UniProtKB-KW
  • negative chemotaxis Source: MGI
  • negative regulation of entry of bacterium into host cell Source: BHF-UCL
  • negative regulation of extrinsic apoptotic signaling pathway Source: MGI
  • negative regulation of lipid storage Source: BHF-UCL
  • negative regulation of lipid transport Source: BHF-UCL
  • negative regulation of lipoprotein metabolic process Source: BHF-UCL
  • negative regulation of low-density lipoprotein particle receptor biosynthetic process Source: BHF-UCL
  • negative regulation of macrophage derived foam cell differentiation Source: BHF-UCL
  • positive regulation of cell adhesion Source: BHF-UCL
  • positive regulation of cell migration Source: BHF-UCL
  • positive regulation of cell proliferation Source: BHF-UCL
  • positive regulation of osteoblast proliferation Source: BHF-UCL
  • regulation of apoptotic cell clearance Source: BHF-UCL
  • regulation of bone resorption Source: BHF-UCL
  • regulation of phagocytosis Source: BHF-UCL
  • release of sequestered calcium ion into cytosol Source: BHF-UCL
  • response to nitric oxide Source: BHF-UCL
  • substrate adhesion-dependent cell spreading Source: MGI
  • T cell activation Source: BHF-UCL
  • trophoblast giant cell differentiation Source: BHF-UCL
  • viral entry into host cell Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Integrin, Receptor

Keywords - Biological processi

Angiogenesis, Cell adhesion, Differentiation

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_286043. Laminin interactions.
REACT_315885. VEGFA-VEGFR2 Pathway.
REACT_319261. Integrin cell surface interactions.
REACT_321869. Cross-presentation of particulate exogenous antigens (phagosomes).
REACT_326610. Syndecan interactions.
REACT_332220. Molecules associated with elastic fibres.
REACT_334317. Elastic fibre formation.
REACT_353319. Signal transduction by L1.
REACT_354321. ECM proteoglycans.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-V
Alternative name(s):
Vitronectin receptor subunit alpha
CD_antigen: CD51
Cleaved into the following 2 chains:
Gene namesi
Name:Itgav
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:96608. Itgav.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini31 – 988958ExtracellularSequence AnalysisAdd
BLAST
Transmembranei989 – 101224HelicalSequence AnalysisAdd
BLAST
Topological domaini1013 – 104432CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • alphav-beta3 integrin-IGF-1-IGF1R complex Source: MGI
  • cell surface Source: BHF-UCL
  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • filopodium membrane Source: MGI
  • focal adhesion Source: MGI
  • integrin alphav-beta3 complex Source: BHF-UCL
  • integrin alphav-beta5 complex Source: MGI
  • integrin alphav-beta8 complex Source: MGI
  • integrin complex Source: BHF-UCL
  • intracellular Source: GOC
  • lamellipodium membrane Source: MGI
  • membrane Source: BHF-UCL
  • microvillus membrane Source: MGI
  • plasma membrane Source: MGI
  • ruffle membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030By similarityAdd
BLAST
Chaini31 – 10441014Integrin alpha-VPRO_0000016304Add
BLAST
Chaini31 – 885855Integrin alpha-V heavy chainBy similarityPRO_0000016305Add
BLAST
Chaini887 – 1044158Integrin alpha-V light chainBy similarityPRO_0000016306Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi74 – 741N-linked (GlcNAc...)2 Publications
Disulfide bondi89 ↔ 97By similarity
Disulfide bondi138 ↔ 158By similarity
Disulfide bondi172 ↔ 185By similarity
Glycosylationi290 – 2901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi296 – 2961N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi491 ↔ 502By similarity
Disulfide bondi508 ↔ 565By similarity
Glycosylationi615 – 6151N-linked (GlcNAc...)2 Publications
Disulfide bondi626 ↔ 632By similarity
Disulfide bondi698 ↔ 711By similarity
Glycosylationi704 – 7041N-linked (GlcNAc...)Sequence Analysis
Glycosylationi835 – 8351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi851 – 8511N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi852 ↔ 900Interchain (between heavy and light chains)By similarity
Glycosylationi869 – 8691N-linked (GlcNAc...)2 Publications
Disulfide bondi905 ↔ 910By similarity
Glycosylationi939 – 9391N-linked (GlcNAc...); atypical1 Publication
Glycosylationi941 – 9411N-linked (GlcNAc...)1 Publication
Glycosylationi969 – 9691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi976 – 9761N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP43406.
PaxDbiP43406.
PRIDEiP43406.

PTM databases

PhosphoSiteiP43406.

Expressioni

Gene expression databases

BgeeiP43406.
CleanExiMM_ITGAV.
ExpressionAtlasiP43406. baseline and differential.
GenevisibleiP43406. MM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-V (ITGAV) associates with either beta-1 (ITGB1), beta-3 (ITGB3), beta-5 (ITGB5), beta-6 (ITGB6) or beta-8 (ITGB8) (Probable). Interacts with RAB25. Interacts with CIB1 (By similarity). Integrins ITGAV:ITGB3 and ITGAV:ITGB5 interact with FBLN5 (via N-terminus)(PubMed:11805835).By similarity1 Publication1 Publication

Protein-protein interaction databases

IntActiP43406. 4 interactions.
MINTiMINT-4098999.
STRINGi10090.ENSMUSP00000028499.

Structurei

3D structure databases

ProteinModelPortaliP43406.
SMRiP43406. Positions 31-993.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati32 – 9867FG-GAP 1Add
BLAST
Repeati109 – 17062FG-GAP 2Add
BLAST
Repeati173 – 22553FG-GAP 3Add
BLAST
Repeati237 – 29559FG-GAP 4Add
BLAST
Repeati296 – 35762FG-GAP 5Add
BLAST
Repeati358 – 41558FG-GAP 6Add
BLAST
Repeati419 – 48264FG-GAP 7Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1015 – 10195GFFKR motif

Sequence similaritiesi

Belongs to the integrin alpha chain family.Curated
Contains 7 FG-GAP repeats.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG26407.
GeneTreeiENSGT00760000118782.
HOGENOMiHOG000231603.
HOVERGENiHBG006186.
InParanoidiP43406.
KOiK06487.
OMAiPSHSKNM.
OrthoDBiEOG7R830R.
TreeFamiTF105391.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
[Graphical view]
PfamiPF01839. FG-GAP. 2 hits.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 5 hits.
[Graphical view]
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPGRLLLR PRPGGLLLLL PGLLLPLADA FNLDVESPAE YAGPEGSYFG
60 70 80 90 100
FAVDFFEPST SSRMFLLVGA PKANTTQPGI VEGGQVLKCE CSSSRRCQPI
110 120 130 140 150
EFDSTGNRDY AKDDPLEFKS HQWFGASVRS KQDKILACAP LYHWRTEMKQ
160 170 180 190 200
EREPVGTCFL QDGTKTVEYA PCRSKNIDAD GQGFCQGGFS IDFTKADRVL
210 220 230 240 250
LGGPGSFYWQ GQLISDQVAE IISKYDPNVY SIKYNNQLAT RTAQAIFDDS
260 270 280 290 300
YLGYSVAVGD FNGDGIEDFV SGVPRAARTL GMVYIYDGKN MSSLHNFTGE
310 320 330 340 350
QMAAYFGFSV AATDINGDDY ADVFIGAPLF MDRGSDGKLQ EVGQVSVSLQ
360 370 380 390 400
RAVGDFQTTK LNGFEVFARF GSAIAPLGDL DQDGFNDIAI AAPYGGEDKK
410 420 430 440 450
GLVYIFNGRS TGLNSVPSQI LEGQWAAQSM PPSFGYSMKG ATDVDRNGYP
460 470 480 490 500
DLVVGAFGVD RAVLYRARPV VTVNAGLEVY PSILNQDNKI CPLPGTALKV
510 520 530 540 550
SCFNVRFCLK ADGKGTLPRK LHFQVELLLD KLKQKGAIRR ALFLHNRSPV
560 570 580 590 600
HSKTMTVFRG GQMQCEELVA YLRDESEFRD KLTPITIFME YRLDQRTAAD
610 620 630 640 650
ATGLQPILNQ FTPANVSRQA HILLDCGEDN VCKPKLEVSV NSDQKKIYIG
660 670 680 690 700
DDNPLTLTVK AQNQGEGAYE AELIVSIPPQ ADFIGVVRNN EALARLSCAF
710 720 730 740 750
KTENQTRQVV CDLGNPMKAG TQLLAGLRFS VHQQSEMDTS VKFDLKIQSS
760 770 780 790 800
NSFDNVSPVV SYKVDLAVLA AVEIRGVSSP DHIFLPIPNW EYKENPETEE
810 820 830 840 850
DVGPIVQHIY ELRNNGPSSF SKAILNLQWP YKYNNNTLLY ILHYDIDGPM
860 870 880 890 900
NCTADTEINP LRIKTPEKND TAAAGQGERN HLITKRDLTL REGDVHTLGC
910 920 930 940 950
GIAKCLQITC QVGRLDRGKS AILYVKSLLW TETFMNKENQ NHSYSLKSSA
960 970 980 990 1000
SFNIIEFPYK NLPIEDLFNS TLVTTNITWG IQPAPMPVPV WVIILAVLAG
1010 1020 1030 1040
LLLLAVLVFV MYRMGFFKRV RPPQEEQERE QLQPHENGEG NSET
Length:1,044
Mass (Da):115,360
Last modified:July 27, 2011 - v2
Checksum:i417FD73D776B5918
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti526 – 5272EL → DV in AAC52497 (PubMed:8601592).Curated
Sequence conflicti768 – 7692VL → EK in AAC52497 (PubMed:8601592).Curated
Sequence conflicti872 – 8721A → G in AAC52497 (PubMed:8601592).Curated
Sequence conflicti880 – 8801N → S in AAC52497 (PubMed:8601592).Curated
Sequence conflicti887 – 8871D → G in AAC52497 (PubMed:8601592).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14135 mRNA. Translation: AAC52497.1.
AL772301 Genomic DNA. Translation: CAM13680.1.
CCDSiCCDS16181.1.
PIRiT10050.
RefSeqiNP_032428.2. NM_008402.3.
UniGeneiMm.227.

Genome annotation databases

EnsembliENSMUST00000028499; ENSMUSP00000028499; ENSMUSG00000027087.
GeneIDi16410.
KEGGimmu:16410.
UCSCiuc008kid.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14135 mRNA. Translation: AAC52497.1.
AL772301 Genomic DNA. Translation: CAM13680.1.
CCDSiCCDS16181.1.
PIRiT10050.
RefSeqiNP_032428.2. NM_008402.3.
UniGeneiMm.227.

3D structure databases

ProteinModelPortaliP43406.
SMRiP43406. Positions 31-993.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP43406. 4 interactions.
MINTiMINT-4098999.
STRINGi10090.ENSMUSP00000028499.

PTM databases

PhosphoSiteiP43406.

Proteomic databases

MaxQBiP43406.
PaxDbiP43406.
PRIDEiP43406.

Protocols and materials databases

DNASUi16410.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028499; ENSMUSP00000028499; ENSMUSG00000027087.
GeneIDi16410.
KEGGimmu:16410.
UCSCiuc008kid.1. mouse.

Organism-specific databases

CTDi3685.
MGIiMGI:96608. Itgav.

Phylogenomic databases

eggNOGiNOG26407.
GeneTreeiENSGT00760000118782.
HOGENOMiHOG000231603.
HOVERGENiHBG006186.
InParanoidiP43406.
KOiK06487.
OMAiPSHSKNM.
OrthoDBiEOG7R830R.
TreeFamiTF105391.

Enzyme and pathway databases

ReactomeiREACT_286043. Laminin interactions.
REACT_315885. VEGFA-VEGFR2 Pathway.
REACT_319261. Integrin cell surface interactions.
REACT_321869. Cross-presentation of particulate exogenous antigens (phagosomes).
REACT_326610. Syndecan interactions.
REACT_332220. Molecules associated with elastic fibres.
REACT_334317. Elastic fibre formation.
REACT_353319. Signal transduction by L1.
REACT_354321. ECM proteoglycans.

Miscellaneous databases

ChiTaRSiItgav. mouse.
NextBioi289589.
PROiP43406.
SOURCEiSearch...

Gene expression databases

BgeeiP43406.
CleanExiMM_ITGAV.
ExpressionAtlasiP43406. baseline and differential.
GenevisibleiP43406. MM.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
[Graphical view]
PfamiPF01839. FG-GAP. 2 hits.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 5 hits.
[Graphical view]
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of mouse integrin alphaV cDNA and role of the alphaV-related matrix receptors in metanephric development."
    Wada J., Kumar A., Liu Z., Ruoslahti E., Reichardt L., Marvaldi J., Kanwar Y.S.
    J. Cell Biol. 132:1161-1176(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD-1.
    Tissue: Kidney.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Cited for: INTERACTION WITH FBLN5.
  4. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74; ASN-615; ASN-869; ASN-939 AND ASN-941.
    Tissue: Myoblast.
  5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74; ASN-615 AND ASN-869.

Entry informationi

Entry nameiITAV_MOUSE
AccessioniPrimary (citable) accession number: P43406
Secondary accession number(s): A2AKI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.