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P43404

- ZAP70_MOUSE

UniProt

P43404 - ZAP70_MOUSE

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Protein
Tyrosine-protein kinase ZAP-70
Gene
Zap70, Srk, Zap-70
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).4 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulationi

Activated by phosphorylation at Tyr-492 in the activation loop.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei368 – 3681ATP By similarity
Active sitei460 – 4601Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi343 – 3519ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: MGI
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  3. phosphotyrosine binding Source: MGI
  4. protein binding Source: UniProtKB
  5. protein kinase activity Source: MGI
  6. protein tyrosine kinase activity Source: MGI
Complete GO annotation...

GO - Biological processi

  1. T cell receptor signaling pathway Source: MGI
  2. beta selection Source: MGI
  3. immune response Source: Ensembl
  4. intracellular signal transduction Source: MGI
  5. negative thymic T cell selection Source: MGI
  6. peptidyl-tyrosine phosphorylation Source: MGI
  7. positive regulation of T cell differentiation Source: MGI
  8. positive regulation of alpha-beta T cell differentiation Source: MGI
  9. positive regulation of alpha-beta T cell proliferation Source: MGI
  10. positive regulation of calcium-mediated signaling Source: MGI
  11. positive thymic T cell selection Source: MGI
  12. protein autophosphorylation Source: MGI
  13. protein phosphorylation Source: MGI
  14. thymic T cell selection Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Adaptive immunity, Immunity

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.
ReactomeiREACT_215414. Translocation of ZAP-70 to Immunological synapse.
REACT_225768. Generation of second messenger molecules.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase ZAP-70 (EC:2.7.10.2)
Alternative name(s):
70 kDa zeta-chain associated protein
Syk-related tyrosine kinase
Gene namesi
Name:Zap70
Synonyms:Srk, Zap-70
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:99613. Zap70.

Subcellular locationi

Cytoplasm. Cell membrane; Peripheral membrane protein
Note: In quiescent T-lymphocytes, ZAP70 is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.2 Publications

GO - Cellular componenti

  1. T cell receptor complex Source: MGI
  2. cell-cell junction Source: MGI
  3. cytoplasm Source: UniProtKB
  4. cytosol Source: MGI
  5. immunological synapse Source: UniProtKB
  6. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice lack both CD4 and CD8 positive mature T-lymphocytes. Displays a complete block in B-Cell development at the pro-B cell stage in the absence of both SYK and ZAP70.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 618618Tyrosine-protein kinase ZAP-70
PRO_0000088169Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei248 – 2481Phosphotyrosine By similarity
Modified residuei287 – 2871Phosphoserine By similarity
Modified residuei290 – 2901Phosphotyrosine By similarity
Modified residuei314 – 3141Phosphotyrosine; by LCK By similarity
Modified residuei318 – 3181Phosphotyrosine By similarity
Modified residuei491 – 4911Phosphotyrosine By similarity

Post-translational modificationi

Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Phosphorylation of Tyr-314 and Tyr-314 are essential for ZAP70 positive function on T-lymphocyte activation whereas Tyr-290 has a negative regulatory role. Within the C-terminal kinase domain, Tyr-491 and Tyr-492 are phosphorylated after TCR induction, Tyr-491 playing a negative regulatory role and Tyr-492 a positive. Tyr-492 is dephosphorylated by PTN22.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43404.
PaxDbiP43404.
PRIDEiP43404.

PTM databases

PhosphoSiteiP43404.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are expressed in thymus, spleen and lymph nodes.1 Publication

Developmental stagei

Isoform 2 is expressed in developing thymocytes from the CD44+CD25- stage up to mature T-cells. Isoform 1 is not expressed in thymocytes at the CD44+CD25- or CD44+CD25+ stages.1 Publication

Gene expression databases

BgeeiP43404.
CleanExiMM_ZAP70.
GenevestigatoriP43404.

Interactioni

Subunit structurei

Interacts with CD3Z; this interaction docks ZAP70 at the stimulated TCR. Interacts with NFAM1. Interacts with adapter proteins SLA and SLA2; these interactions negatively regulates T-cell receptor signaling. Interacts with CBLB. Interacts with CBL; this interaction promotes ubiquitination, internalization and subsequent degradation of CD3Z. Identified in a complex with CBL and UBE2L3. Interacts with DEF6. Interacts (via SH2 domains) with RHOH; this interaction regulates ZAP70 subcellular localization. Interacts with FCRL3. Interacts with VAV1 By similarity.6 Publications

Protein-protein interaction databases

IntActiP43404. 3 interactions.
STRINGi10090.ENSMUSP00000027291.

Structurei

3D structure databases

ProteinModelPortaliP43404.
SMRiP43404. Positions 1-605.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 10293SH2 1
Add
BLAST
Domaini163 – 25492SH2 2
Add
BLAST
Domaini337 – 597261Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni103 – 16260Interdomain A
Add
BLAST
Regioni255 – 33682Interdomain B
Add
BLAST

Domaini

Composed of 2 N-terminal SH2 domains and a C-terminal kinase domain. The tandem SH2 domains bind to the doubly phosphorylated tyrosine-based activation motif (ITAM) of CD247/CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH By similarity. The interdomain B located between the second SH2 and the kinase domain has been implicated in binding to other signaling molecules including CBL or VAV1. Thus, ZAP70 can also function as a scaffold by recruiting additional factors to the stimulated TCR complex By similarity.1 Publication

Sequence similaritiesi

Contains 2 SH2 domains.

Keywords - Domaini

Repeat, SH2 domain

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00740000115195.
HOGENOMiHOG000113264.
HOVERGENiHBG001540.
InParanoidiP43404.
KOiK07360.
OMAiMKGPEVI.
OrthoDBiEOG7MWGWD.
TreeFamiTF351629.

Family and domain databases

Gene3Di1.10.930.10. 1 hit.
3.30.505.10. 2 hits.
InterProiIPR011009. Kinase-like_dom.
IPR023420. Kinase_SYK/ZAP-70_inter-SH2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR012234. Tyr_kinase_non-rcpt_SYK/ZAP70.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 2 hits.
[Graphical view]
PIRSFiPIRSF000604. TyrPK_SYK. 1 hit.
PRINTSiPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P43404-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPDPAAHLPF FYGSISRAEA EEHLKLAGMA DGLFLLRQCL RSLGGYVLSL    50
VHDVRFHHFP IERQLNGTYA IAGGKAHCGP AELCQFYSQD PDGLPCNLRK 100
PCNRPPGLEP QPGVFDCLRD AMVRDYVRQT WKLEGDALEQ AIISQAPQVE 150
KLIATTAHER MPWYHSSLTR EEAERKLYSG QQTDGKFLLR PRKEQGTYAL 200
SLVYGKTVYH YLISQDKAGK YCIPEGTKFD TLWQLVEYLK LKADGLIYRL 250
KEVCPNSSAS AAVAAPTLPA HPSTFTQPQR RVDTLNSDGY TPEPARLASS 300
TDKPRPMPMD TSVYESPYSD PEELKDKKLF LKRENLLVAD IELGCGNFGS 350
VRQGVYRMRK KQIDVAIKVL KQGTEKADKD EMMREAQIMH QLDNPYIVRL 400
IGVCQAEALM LVMEMAGGGP LHKFLLGKKE EIPVSNVAEL LHQVAMGMKY 450
LEEKNFVHRD LAARNVLLVN RHYAKISDFG LSKALGADDS YYTARSAGKW 500
PLKWYAPECI NFRKFSSRSD VWSYGVTMWE AFSYGQKPYK KMKGPEVLDF 550
IKQGKRMECP PECPPEMYAL MSDCWIYKWE DRPDFLTVEQ RMRNYYYSLA 600
SRAEGPPQCE QVAEAACG 618
Length:618
Mass (Da):70,112
Last modified:December 14, 2011 - v3
Checksum:i9E0D18B240B28392
GO
Isoform 2 (identifier: P43404-2) [UniParc]FASTAAdd to Basket

Also known as: TZK

The sequence of this isoform differs from the canonical sequence as follows:
     1-306: Missing.

Show »
Length:312
Mass (Da):35,883
Checksum:i12CCA3AA2C3B1CF2
GO
Isoform 3 (identifier: P43404-3) [UniParc]FASTAAdd to Basket

Also known as: TZK-2

The sequence of this isoform differs from the canonical sequence as follows:
     1-309: Missing.
     310-360: DTSVYESPYS...VRQGVYRMRK → MAYGRVSGVS...MLSVASHLGR

Note: No experimental confirmation available.

Show »
Length:309
Mass (Da):35,255
Checksum:iD1A230395CA2AFE4
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti464 – 4641R → C in ST; causes an absence of mature T-cells due to thymocyte development being arrested at the CD4+CD8+ stage. 1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 309309Missing in isoform 3.
VSP_031160Add
BLAST
Alternative sequencei1 – 306306Missing in isoform 2.
VSP_031159Add
BLAST
Alternative sequencei310 – 36051DTSVY…YRMRK → MAYGRVSGVSELSRVLYVPF PPPPFLSNPGVHDTRMYTQH AMLSVASHLGR in isoform 3.
VSP_031161Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti124 – 1241R → C in AAA19250. 1 Publication
Sequence conflicti526 – 5294VTMW → GHHV in BAC67015. 1 Publication
Sequence conflicti546 – 5461E → Q in AAA19250. 1 Publication
Sequence conflicti599 – 5991L → P in AAA19250. 1 Publication
Sequence conflicti599 – 5991L → P in AAB36538. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U04379 mRNA. Translation: AAA19250.1.
U77667 mRNA. Translation: AAB36538.1.
AB083210 mRNA. Translation: BAC43746.1.
AB084383 mRNA. Translation: BAC67015.1.
AK039883 mRNA. Translation: BAC30471.1.
AC084389 mRNA. No translation available.
BC029727 mRNA. Translation: AAH29727.1.
CCDSiCCDS14888.1. [P43404-1]
PIRiI48914.
RefSeqiNP_001276541.1. NM_001289612.1. [P43404-2]
NP_001276694.1. NM_001289765.1. [P43404-1]
NP_033565.2. NM_009539.3. [P43404-1]
XP_006495959.1. XM_006495896.1. [P43404-1]
UniGeneiMm.8038.

Genome annotation databases

EnsembliENSMUST00000027291; ENSMUSP00000027291; ENSMUSG00000026117. [P43404-1]
GeneIDi22637.
KEGGimmu:22637.
UCSCiuc007aqz.1. mouse. [P43404-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U04379 mRNA. Translation: AAA19250.1 .
U77667 mRNA. Translation: AAB36538.1 .
AB083210 mRNA. Translation: BAC43746.1 .
AB084383 mRNA. Translation: BAC67015.1 .
AK039883 mRNA. Translation: BAC30471.1 .
AC084389 mRNA. No translation available.
BC029727 mRNA. Translation: AAH29727.1 .
CCDSi CCDS14888.1. [P43404-1 ]
PIRi I48914.
RefSeqi NP_001276541.1. NM_001289612.1. [P43404-2 ]
NP_001276694.1. NM_001289765.1. [P43404-1 ]
NP_033565.2. NM_009539.3. [P43404-1 ]
XP_006495959.1. XM_006495896.1. [P43404-1 ]
UniGenei Mm.8038.

3D structure databases

ProteinModelPortali P43404.
SMRi P43404. Positions 1-605.
ModBasei Search...

Protein-protein interaction databases

IntActi P43404. 3 interactions.
STRINGi 10090.ENSMUSP00000027291.

Chemistry

ChEMBLi CHEMBL2034801.

PTM databases

PhosphoSitei P43404.

Proteomic databases

MaxQBi P43404.
PaxDbi P43404.
PRIDEi P43404.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027291 ; ENSMUSP00000027291 ; ENSMUSG00000026117 . [P43404-1 ]
GeneIDi 22637.
KEGGi mmu:22637.
UCSCi uc007aqz.1. mouse. [P43404-1 ]

Organism-specific databases

CTDi 7535.
MGIi MGI:99613. Zap70.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00740000115195.
HOGENOMi HOG000113264.
HOVERGENi HBG001540.
InParanoidi P43404.
KOi K07360.
OMAi MKGPEVI.
OrthoDBi EOG7MWGWD.
TreeFami TF351629.

Enzyme and pathway databases

BRENDAi 2.7.10.2. 3474.
Reactomei REACT_215414. Translocation of ZAP-70 to Immunological synapse.
REACT_225768. Generation of second messenger molecules.

Miscellaneous databases

NextBioi 303021.
PROi P43404.
SOURCEi Search...

Gene expression databases

Bgeei P43404.
CleanExi MM_ZAP70.
Genevestigatori P43404.

Family and domain databases

Gene3Di 1.10.930.10. 1 hit.
3.30.505.10. 2 hits.
InterProi IPR011009. Kinase-like_dom.
IPR023420. Kinase_SYK/ZAP-70_inter-SH2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR012234. Tyr_kinase_non-rcpt_SYK/ZAP70.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 2 hits.
[Graphical view ]
PIRSFi PIRSF000604. TyrPK_SYK. 1 hit.
PRINTSi PR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTi SM00252. SH2. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF55550. SSF55550. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Interactions of p59fyn and ZAP-70 with T-cell receptor activation motifs: defining the nature of a signalling motif."
    Gauen L.K.T., Zhu Y., Letourner F., Hu Q., Bolen J.B., Matis L.A., Klausner R.D., Shaw A.S.
    Mol. Cell. Biol. 14:3729-3741(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Thymus.
  2. "A spontaneously arising mutation in the DLAARN motif of murine ZAP-70 abrogates kinase activity and arrests thymocyte development."
    Wiest D.L., Ashe J.M., Howcroft T.K., Lee H.-M., Kemper D.M., Negishi I., Singer D.S., Singer A., Abe R.
    Immunity 6:663-671(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ST CYS-464.
    Strain: C57BL/6.
    Tissue: Thymocyte.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6.
    Tissue: Thymus.
  4. "Mouse TZK-2."
    Ikeda T., Kuroyama H.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Thymus.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Salivary gland.
  8. "Essential role for ZAP-70 in both positive and negative selection of thymocytes."
    Negishi I., Motoyama N., Nakayama K., Nakayama K., Senju S., Hatakeyama S., Zhang Q., Chan A.C., Loh D.Y.
    Nature 376:435-438(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  9. "Src-like adaptor protein (SLAP) is a negative regulator of T cell receptor signaling."
    Sosinowski T., Pandey A., Dixit V.M., Weiss A.
    J. Exp. Med. 191:463-474(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SLA.
  10. Cited for: INTERACTION WITH CBLB.
  11. "A novel Src homology 2 domain-containing molecule, Src-like adapter protein-2 (SLAP-2), which negatively regulates T cell receptor signaling."
    Pandey A., Ibarrola N., Kratchmarova I., Fernandez M.M., Constantinescu S.N., Ohara O., Sawasdikosol S., Lodish H.F., Mann M.
    J. Biol. Chem. 277:19131-19138(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SLA2.
  12. "SWAP-70-like adapter of T cells, an adapter protein that regulates early TCR-initiated signaling in Th2 lineage cells."
    Tanaka Y., Bi K., Kitamura R., Hong S., Altman Y., Matsumoto A., Tabata H., Lebedeva S., Bushway P.J., Altman A.
    Immunity 18:403-414(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DEF6.
  13. "Unexpected requirement for ZAP-70 in pre-B cell development and allelic exclusion."
    Schweighoffer E., Vanes L., Mathiot A., Nakamura T., Tybulewicz V.L.
    Immunity 18:523-533(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  14. "NFAM1, an immunoreceptor tyrosine-based activation motif-bearing molecule that regulates B cell development and signaling."
    Ohtsuka M., Arase H., Takeuchi A., Yamasaki S., Shiina R., Suenaga T., Sakurai D., Yokosuka T., Arase N., Iwashima M., Kitamura T., Moriya H., Saito T.
    Proc. Natl. Acad. Sci. U.S.A. 101:8126-8131(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NFAM1.
  15. "RhoH GTPase recruits and activates Zap70 required for T cell receptor signaling and thymocyte development."
    Gu Y., Chae H.-D., Siefring J.E., Jasti A.C., Hildeman D.A., Williams D.A.
    Nat. Immunol. 7:1182-1190(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH RHOH, DOMAIN.
  16. "Distinct roles for Syk and ZAP-70 during early thymocyte development."
    Palacios E.H., Weiss A.
    J. Exp. Med. 204:1703-1715(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN THYMOCYTE DEVELOPMENT.

Entry informationi

Entry nameiZAP70_MOUSE
AccessioniPrimary (citable) accession number: P43404
Secondary accession number(s): P97455, Q80VV2, Q8CHJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 14, 2011
Last modified: September 3, 2014
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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