Reviewed, UniProtKB/Swiss-Prot P43387 (DCMA_METS1)

Names and origin

Protein names

Recommended name:
    Dichloromethane dehalogenase
      Short name=DCM dehalogenase
    EC=4.5.1.3

Gene names

Name:

dcmA

Organism

Methylophilus sp. (strain DM11)

Taxonomic identifier

45393 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylophilus

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Dichloromethane + H2O = formaldehyde + 2 HCl.
Pathway	Xenobiotic degradation; dichloromethane degradation.
Subunit structure	Homohexamer.
Subcellular location	Cytoplasm Probable.
Induction	By dichloromethane.
Sequence similarities	Belongs to the GST superfamily. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dichloromethane dehalogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1 Ref.2
	Chain	2 – 267	266	Dichloromethane dehalogenase		PRO_0000185989
Regions
	Domain	3 – 85	83	GST N-terminal
	Domain	91 – 224	134	GST C-terminal

Sequences

	Sequence		Length	Mass (Da)
	P43387-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: BCFE1606136CACA1	FASTA	267	31,167
	10 20 30 40 50 60 MSTKLRYLHH PASQPCRAVH QFMLENNIEF QEEIVDITTD INEQPEFRER YNPTGQVPIL 70 80 90 100 110 120 VDGDFTIWES AAIVYYLSEK YDCSSSWWGS TLEERGHIQQ YMHWYAYTLR LGGGAFHWTI 130 140 150 160 170 180 FAPMIYGYDK DFTVEVTKGR FLLYESFDIL EKYWLKDGDY LCGNTLSYPD LATCQDLVSH 190 200 210 220 230 240 DAGRIIPTSM WDSHPKVKAW FARMMDREHA KTVSAWQYEN VRKYLDDGVK LNFQRKTAVL 250 260 KGTEVYSGHN NGIIYNGDDD SFVTQHG

References

[1]	"Isolation and characterization of the Methylophilus sp. strain DM11 gene encoding dichloromethane dehalogenase/glutathione S-transferase." Bader R., Leisinger T. J. Bacteriol. 176:3466-3473(1994) [PubMed: 8206823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-31.
[2]	"Dichloromethane dehalogenase with improved catalytic activity isolated from a fast-growing dichloromethane-utilizing bacterium." Scholtz R., Wackett L.P., Egli C., Cook A.M., Leisinger T. J. Bacteriol. 170:5698-5704(1988) [PubMed: 3142855] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-23.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L26544 Genomic DNA. Translation: AAA25443.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCMA_METS1

Accession

Primary (citable) accession number: P43387

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)