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Protein

Glutamine synthetase

Gene

glnA

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Glutamine synthetase (GS) is an unusual multitasking protein that functions as an enzyme, a transcription coregulator, and a chaperone in ammonium assimilation and in the regulation of genes involved in nitrogen metabolism. It catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Feedback-inhibited GlnA also interacts with and regulates the activity of the transcriptional regulator TnrA. During nitrogen limitation, TnrA is in its DNA-binding active state and turns on the transcription of genes required for nitrogen assimilation. Under conditions of nitrogen excess, feedback-inhibited GlnA forms a stable complex with TnrA, which inhibits its DNA-binding activity. In contrast, feedback-inhibited GlnA acts as a chaperone to stabilize the DNA-binding activity of GlnR, which represses the transcription of nitrogen assimilation genes.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei72Important for inhibition by glutamineBy similarity1
Metal bindingi143Magnesium 1By similarity1
Metal bindingi145Magnesium 2By similarity1
Binding sitei195ATPBy similarity1
Metal bindingi200Magnesium 2By similarity1
Metal bindingi207Magnesium 2By similarity1
Binding sitei252L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi256Magnesium 1; via pros nitrogenBy similarity1
Binding sitei260ATPBy similarity1
Binding sitei309L-glutamateBy similarity1
Binding sitei315L-glutamateBy similarity1
Binding sitei327ATPBy similarity1
Binding sitei327L-glutamateBy similarity1
Binding sitei332ATPBy similarity1
Metal bindingi345Magnesium 1By similarity1
Binding sitei347L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi258 – 260ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I alphaBy similarity
Short name:
GSI alphaBy similarity
Gene namesi
Name:glnABy similarity
Ordered Locus Names:HVO_0239
OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Taxonomic identifieri309800 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHaloferacalesHaloferacaceaeHaloferax
Proteomesi
  • UP000008243 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532031 – 456Glutamine synthetaseAdd BLAST456

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP43386

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons. In its feedback-inhibited form, interacts with TnrA in order to block its DNA-binding activity.By similarity

Protein-protein interaction databases

STRINGi309800.HVO_0239

Structurei

3D structure databases

SMRiP43386
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni251 – 252L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiarCOG01909 Archaea
COG0174 LUCA
HOGENOMiHOG000005156
KOiK01915
OMAiIEAAWNT
OrthoDBiPOG093Z00GE

Family and domain databases

Gene3Di3.10.20.70, 1 hit
InterProiView protein in InterPro
IPR008147 Gln_synt_b-grasp
IPR036651 Gln_synt_N
IPR014746 Gln_synth/guanido_kin_cat_dom
IPR008146 Gln_synth_cat_dom
IPR027303 Gln_synth_gly_rich_site
IPR004809 Gln_synth_I
IPR027302 Gln_synth_N_conserv_site
PfamiView protein in Pfam
PF00120 Gln-synt_C, 1 hit
PF03951 Gln-synt_N, 1 hit
SMARTiView protein in SMART
SM01230 Gln-synt_C, 1 hit
SUPFAMiSSF54368 SSF54368, 1 hit
SSF55931 SSF55931, 1 hit
TIGRFAMsiTIGR00653 GlnA, 1 hit
PROSITEiView protein in PROSITE
PS00180 GLNA_1, 1 hit
PS00181 GLNA_ATP, 1 hit

Sequencei

Sequence statusi: Complete.

P43386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEDNALTDG GLSDEAQAVI DEIEEKNVDF LRLQFTDILG TVKNVSIPAS
60 70 80 90 100
QAEKAFTEGI YFDGSSIDGF VRIQESDMRL EPDPSTFAVL PWRKKENSAA
110 120 130 140 150
GRLICDVFNT STGEPFSGDP RGVLKRAIER AEELGYDVNV APEPEFFLFE
160 170 180 190 200
EDEDGRATTV TNDAGGYFDL APKDLASDVR RDIIYGLESM GFDIEASHHE
210 220 230 240 250
VAEGQHEINF TYDDALSTAD NVATFRSVVR AIAAEHDLHA TFMPKPIPRI
260 270 280 290 300
NGSGMHTHIS LFKDGENAFH DGDDEFDLSD TAKSFVAGIL DHAPAITAVA
310 320 330 340 350
DPTVNSYKRL VPGYEAPVYI AWSDRNRSAL IRKPAARTPA ASRIEARFPD
360 370 380 390 400
PSCNPYLAFA ALIHAGLDGV EKGLDCPDPV RENIYEFDEA KREEYGIETL
410 420 430 440 450
PKDLGGAVDA LEEDEVIQEA LGDHVFEKFV EAKRSEFKDY LVDVSQWELD

RYLETF
Length:456
Mass (Da):50,522
Last modified:July 13, 2010 - v2
Checksum:i7A3905E9706174EA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti130 – 131RA → P in AAC43489 (PubMed:7916055).Curated2
Sequence conflicti223A → R in AAC43489 (PubMed:7916055).Curated1
Sequence conflicti299 – 308VADPTVNSYK → SLTRRSTPTR in AAC43489 (PubMed:7916055).Curated10
Sequence conflicti403 – 413DLGGAVDALEE → TSAARRRPRR in AAC43489 (PubMed:7916055).CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03029 Genomic DNA Translation: AAC43489.1
CP001956 Genomic DNA Translation: ADE03696.1
PIRiT47127
RefSeqiWP_004045354.1, NZ_AOHU01000107.1

Genome annotation databases

EnsemblBacteriaiADE03696; ADE03696; HVO_0239
GeneIDi8924457
KEGGihvo:HVO_0239

Similar proteinsi

Entry informationi

Entry nameiGLN1A_HALVD
AccessioniPrimary (citable) accession number: P43386
Secondary accession number(s): D4GZI4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 13, 2010
Last modified: May 23, 2018
This is version 92 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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