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P43386 (GLNA_HALVD) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase

Short name=GS
EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase
Gene names
Name:glnA
Ordered Locus Names:HVO_0239
OrganismHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) [Reference proteome] [HAMAP]
Taxonomic identifier309800 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax

Protein attributes

Sequence length456 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulation

The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 456456Glutamine synthetase
PRO_0000153203

Amino acid modifications

Modified residue3851O-AMP-tyrosine By similarity

Experimental info

Sequence conflict130 – 1312RA → P in AAC43489. Ref.1
Sequence conflict2231A → R in AAC43489. Ref.1
Sequence conflict299 – 30810VADPTVNSYK → SLTRRSTPTR in AAC43489. Ref.1
Sequence conflict403 – 41311DLGGAVDALEE → TSAARRRPRR in AAC43489. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P43386 [UniParc].

Last modified July 13, 2010. Version 2.
Checksum: 7A3905E9706174EA

FASTA45650,522
        10         20         30         40         50         60 
MTEDNALTDG GLSDEAQAVI DEIEEKNVDF LRLQFTDILG TVKNVSIPAS QAEKAFTEGI 

        70         80         90        100        110        120 
YFDGSSIDGF VRIQESDMRL EPDPSTFAVL PWRKKENSAA GRLICDVFNT STGEPFSGDP 

       130        140        150        160        170        180 
RGVLKRAIER AEELGYDVNV APEPEFFLFE EDEDGRATTV TNDAGGYFDL APKDLASDVR 

       190        200        210        220        230        240 
RDIIYGLESM GFDIEASHHE VAEGQHEINF TYDDALSTAD NVATFRSVVR AIAAEHDLHA 

       250        260        270        280        290        300 
TFMPKPIPRI NGSGMHTHIS LFKDGENAFH DGDDEFDLSD TAKSFVAGIL DHAPAITAVA 

       310        320        330        340        350        360 
DPTVNSYKRL VPGYEAPVYI AWSDRNRSAL IRKPAARTPA ASRIEARFPD PSCNPYLAFA 

       370        380        390        400        410        420 
ALIHAGLDGV EKGLDCPDPV RENIYEFDEA KREEYGIETL PKDLGGAVDA LEEDEVIQEA 

       430        440        450 
LGDHVFEKFV EAKRSEFKDY LVDVSQWELD RYLETF 

« Hide

References

« Hide 'large scale' references
[1]"Evolutionary relationships of bacterial and archaeal glutamine synthetase genes."
Brown J.R., Masuchi Y., Robb F.T., Doolittle W.F.
J. Mol. Evol. 38:566-576(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete genome sequence of Haloferax volcanii DS2, a model archaeon."
Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R., Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J., Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.
PLoS ONE 5:E9605-E9605(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U03029 Genomic DNA. Translation: AAC43489.1.
CP001956 Genomic DNA. Translation: ADE03696.1.
PIRT47127.
RefSeqYP_003534316.1. NC_013967.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADE03696; ADE03696; HVO_0239.
GeneID8924457.
KEGGhvo:HVO_0239.

Phylogenomic databases

HOGENOMHOG000005156.
KOK01915.
OMAFGEGTYA.
ProtClustDBCLSK511606.

Enzyme and pathway databases

BioCycHVOL309800:GCOK-243-MONOMER.

Family and domain databases

Gene3D3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
TIGRFAMsTIGR00653. GlnA. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA_HALVD
AccessionPrimary (citable) accession number: P43386
Secondary accession number(s): D4GZI4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 13, 2010
Last modified: November 13, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families