P43378 (PTN9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase non-receptor type 9
EC=3.1.3.48

Alternative name(s):
Protein-tyrosine phosphatase MEG2
Short name=PTPase MEG2

Gene names

Name:

PTPN9

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein-tyrosine phosphatase that could participate in the transfer of hydrophobic ligands or in functions of the Golgi apparatus. Ref.6
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class 3 subfamily. Contains 1 CRAL-TRIO domain. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Protein phosphatase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasmic part Inferred from direct assay PubMed 10940933. Source: UniProtKB
Molecular_function	non-membrane spanning protein tyrosine phosphatase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GHR	P10912	2	EBI-742898,EBI-286316

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 593

593

Tyrosine-protein phosphatase non-receptor type 9

PRO_0000094764

Regions

Domain

84 – 243

160

CRAL-TRIO

Domain

303 – 574

272

Tyrosine-protein phosphatase

Region

515 – 521

7

Substrate binding By similarity

Sites

Active site

515

1

Phosphocysteine intermediate By similarity

Binding site

470

1

Substrate By similarity

Binding site

559

1

Substrate By similarity

Secondary structure

1

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593

Helix Strand Turn

Details...

Helix

288 – 312

25

Helix

320 – 323

4

Turn

325 – 327

3

Helix

328 – 330

3

Turn

340 – 342

3

Beta strand

343 – 345

3

Beta strand

350 – 352

3

Beta strand

359 – 365

7

Beta strand

368 – 375

8

Helix

380 – 382

3

Helix

383 – 392

10

Beta strand

397 – 400

4

Beta strand

404 – 406

3

Beta strand

423 – 426

4

Beta strand

429 – 438

10

Beta strand

440 – 451

12

Turn

452 – 455

4

Beta strand

456 – 465

10

Beta strand

470 – 472

3

Helix

478 – 497

20

Beta strand

511 – 514

4

Beta strand

516 – 519

4

Helix

520 – 538

19

Beta strand

539 – 541

3

Helix

543 – 550

8

Turn

551 – 553

3

Helix

561 – 577

17

Sequences

	Sequence		Length	Mass (Da)
	P43378 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 9BD75A5A986DDA0B	FASTA	593	68,020
	10 20 30 40 50 60 MEPATAPRPD MAPELTPEEE QATKQFLEEI NKWTVQYNVS PLSWNVAVKF LMARKFDVLR 70 80 90 100 110 120 AIELFHSYRE TRRKEGIVKL KPHEEPLRSE ILSGKFTILN VRDPTGASIA LFTARLHHPH 130 140 150 160 170 180 KSVQHVVLQA LFYLLDRAVD SFETQRNGLV FIYDMCGSNY ANFELDLGKK VLNLLKGAFP 190 200 210 220 230 240 ARLKKVLIVG APIWFRVPYS IISLLLKDKV RERIQILKTS EVTQHLPREC LPENLGGYVK 250 260 270 280 290 300 IDLATWNFQF LPQVNGHPDP FDEIILFSLP PALDWDSVHV PGPHAMTIQE LVDYVNARQK 310 320 330 340 350 360 QGIYEEYEDI RRENPVGTFH CSMSPGNLEK NRYGDVPCLD QTRVKLTKRS GHTQTDYINA 370 380 390 400 410 420 SFMDGYKQKN AYIGTQGPLE NTYRDFWLMV WEQKVLVIVM TTRFEEGGRR KCGQYWPLEK 430 440 450 460 470 480 DSRIRFGFLT VTNLGVENMN HYKKTTLEIH NTEERQKRQV THFQFLSWPD YGVPSSAASL 490 500 510 520 530 540 IDFLRVVRNQ QSLAVSNMGA RSKGQCPEPP IVVHCSAGIG RTGTFCSLDI CLAQLEELGT 550 560 570 580 590 LNVFQTVSRM RTQRAFSIQT PEQYYFCYKA ILEFAEKEGM VSSGQNLLAV ESQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a cytosolic megakaryocyte protein-tyrosine-phosphatase with sequence homology to retinaldehyde-binding protein and yeast SEC14p." Gu M., Warshawsky I., Majerus P.W. Proc. Natl. Acad. Sci. U.S.A. 89:2980-2984(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]	"Large-scale structural analysis of the classical human protein tyrosine phosphatome." Barr A.J., Ugochukwu E., Lee W.H., King O.N.F., Filippakopoulos P., Alfano I., Savitsky P., Burgess-Brown N.A., Mueller S., Knapp S. Cell 136:352-363(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 277-582, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M83738 mRNA. Translation: AAA60226.1.
BT007405 mRNA. Translation: AAP36073.1.
BC010863 mRNA. Translation: AAH10863.1.

IPI

IPI00852804.

PIR

A42690.

RefSeq

NP_002824.1. NM_002833.2.

UniGene

Hs.445775.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PA5	X-ray	1.60	A/B	277-582	[»]
4GE2	X-ray	1.80	A/B	277-582	[»]
4GE5	X-ray	2.00	A/B	277-582	[»]
4GE6	X-ray	1.40	A/B	277-582	[»]

ProteinModelPortal

P43378.

ModBase

Search...

Protein-protein interaction databases

IntAct

P43378. 3 interactions.

MINT

MINT-1455219.

STRING

9606.ENSP00000303554.

PTM databases

PhosphoSite

P43378.

Polymorphism databases

DMDM

1172724.

Proteomic databases

PaxDb

P43378.

PRIDE

P43378.

Protocols and materials databases

DNASU

5780.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000306726; ENSP00000303554; ENSG00000169410.

GeneID

5780.

KEGG

hsa:5780.

UCSC

uc002bal.3. human.

Organism-specific databases

CTD

5780.

GeneCards

GC15M075759.

HGNC

HGNC:9661. PTPN9.

HPA

HPA041922.

MIM

600768. gene.

neXtProt

NX_P43378.

PharmGKB

PA34005.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5599.

HOGENOM

HOG000006971.

HOVERGEN

HBG006880.

InParanoid

P43378.

KO

K01104.

OMA

MTTRFEE.

OrthoDB

EOG4DJJW7.

PhylomeDB

P43378.

Gene expression databases

ArrayExpress

P43378.

Bgee

P43378.

CleanEx

HS_PTPN9.

Genevestigator

P43378.

GermOnline

ENSG00000169410. Homo sapiens.

Family and domain databases

Gene3D

3.40.525.10. 1 hit.

InterPro

IPR001251. CRAL-TRIO_dom.
IPR011074. CRAL/TRIO_N_dom.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]

Pfam

PF00650. CRAL_TRIO. 1 hit.
PF03765. CRAL_TRIO_N. 1 hit.
PF00102. Y_phosphatase. 1 hit.
[Graphical view]

PRINTS

PR00700. PRTYPHPHTASE.

SMART

SM01100. CRAL_TRIO_N. 1 hit.
SM00194. PTPc. 1 hit.
SM00516. SEC14. 1 hit.
[Graphical view]

SUPFAM

SSF52087. CRAL_TRIO_C. 1 hit.
SSF46938. Sec14p_like_N. 1 hit.

PROSITE

PS50191. CRAL_TRIO. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P43378.

ChEMBL

CHEMBL6117.

ChiTaRS

PTPN9. human.

EvolutionaryTrace

P43378.

GenomeRNAi

5780.

NextBio

22478.

SOURCE

Search...

Entry information

Entry name

PTN9_HUMAN

Accession

Primary (citable) accession number: P43378
Secondary accession number(s): Q53XR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 132 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.