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Protein

Calpain-3

Gene

CAPN3

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease.

Catalytic activityi

Broad endopeptidase activity.

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei128By similarity1
Active sitei334By similarity1
Active sitei358By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi705 – 7161CuratedAdd BLAST12
Calcium bindingi735 – 7462CuratedAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.54. 6170.

Protein family/group databases

MEROPSiC02.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-3 (EC:3.4.22.54)
Alternative name(s):
Calcium-activated neutral proteinase 3
Short name:
CANP 3
Calpain L3
Calpain p94
Muscle-specific calcium-activated neutral protease 3
New calpain 1
Short name:
nCL-1
Gene namesi
Name:CAPN3
Synonyms:NCL1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002077091 – 821Calpain-3Add BLAST821

Proteomic databases

PaxDbiP43368.
PeptideAtlasiP43368.
PRIDEiP43368.

Expressioni

Tissue specificityi

Skeletal muscle.

Interactioni

Subunit structurei

Interacts with TTN/titin. Interacts with CMYA5; this interaction, which results in CMYA5 proteolysis, may protect CAPN3 from autolysis.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000005095.

Structurei

3D structure databases

ProteinModelPortaliP43368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 417Calpain catalyticPROSITE-ProRule annotationAdd BLAST345
Domaini649 – 683EF-hand 1PROSITE-ProRule annotationAdd BLAST35
Domaini692 – 725EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini722 – 757EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini787 – 821EF-hand 4PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni418 – 586Domain IIIAdd BLAST169
Regioni587 – 649LinkerAdd BLAST63
Regioni650 – 821Domain IVAdd BLAST172

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiP43368.
KOiK08573.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029531. CAPN3.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF320. PTHR10183:SF320. 2 hits.
PfamiPF01067. Calpain_III. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43368-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTVISASMA PRTGASQVPR TMPQAAQGKG TEAGVGNPGG KYSAIISRNF
60 70 80 90 100
PIIGVKEKTF EQLHKKCLEK KVLYLDPEFP PDETSLFYSQ KFPIQFVWKR
110 120 130 140 150
PPEICENPRF IIGGANRTDI CQGDLGDCWF LAAIACLTLN KRLLFRVIPH
160 170 180 190 200
DQSFTENYAG IFHFQFWRYG DWVDVVIDDC LPTYNNQLVF TKSNHRNEFW
210 220 230 240 250
SALLEKAYAK LHGSYEALKG GNTTEAMEDF TGGVTEFFEI KDAPRDMYKI
260 270 280 290 300
MKKAIERGSL MGCSIDDGTN MTYGTSPSGL KMGDLIARMV RNMDESRLRD
310 320 330 340 350
SDLIPEGCSD DRPTRTIVPV QFETRMACGL VKGHAYSVTG LEEALFKGEK
360 370 380 390 400
VKLVRLRNPW GQVEWNGSWS DSWKDWSFVD KDEKARLQHQ VTEDGEFWMS
410 420 430 440 450
YDDFIYHFTK LEICNLTADA LESDKLQTWT VSVNEGRWVR GCSAGGCRNF
460 470 480 490 500
PDTFWTNPQY RLKLLEEDDD PDDSEVICSF LVALMQKNRR KDRKLGANLF
510 520 530 540 550
TIGFAIYEVP KEMHGNKQHL QKDFFLYNAS KARSRTYINM REVSERFRLP
560 570 580 590 600
PSEYVIVPST YEPHQEGEFI LRVFSEKRNL SEEVENTISV DRPVRKKKTK
610 620 630 640 650
PIIFVSDRAN SNKELGVDQE SEEGQDKTSP DKQEKSPKPE PSNTDQESEE
660 670 680 690 700
QQQFRNIFRQ IAGDDMEICA DELKNVLNRV VNKHKDLKTE GFTLESCRSM
710 720 730 740 750
IALMDTDGSG RLNLQEFHHL WKKIKSWQKI FKHYDTDQSG TINSYEMRNA
760 770 780 790 800
VNDAGFHLNN QLYDIITMRY ADKYMNIDFD SFICCFVRLE GMFRAFNAFD
810 820
KDGDGIIKLN VLEWLQLTMY A
Length:821
Mass (Da):94,551
Last modified:June 16, 2003 - v2
Checksum:i868E2769AD3AAFDF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti281K → N in AAA17032 (Ref. 2) Curated1
Sequence conflicti307 – 308GC → V in AAA17032 (Ref. 2) Curated2
Sequence conflicti446 – 447GC → TG in AAA17032 (Ref. 2) Curated2
Sequence conflicti446G → A in AAA67685 (PubMed:8833248).Curated1
Sequence conflicti487K → R in AAA17032 (Ref. 2) Curated1
Sequence conflicti570I → M in AAA17032 (Ref. 2) Curated1
Sequence conflicti579N → K in AAA17032 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043295 mRNA. Translation: AAB99847.1.
U05678 mRNA. Translation: AAA17032.1.
U23954 Genomic DNA. Translation: AAA67685.1.
RefSeqiNP_999336.1. NM_214171.1.
UniGeneiSsc.16057.

Genome annotation databases

GeneIDi397349.
KEGGissc:397349.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043295 mRNA. Translation: AAB99847.1.
U05678 mRNA. Translation: AAA17032.1.
U23954 Genomic DNA. Translation: AAA67685.1.
RefSeqiNP_999336.1. NM_214171.1.
UniGeneiSsc.16057.

3D structure databases

ProteinModelPortaliP43368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000005095.

Protein family/group databases

MEROPSiC02.004.

Proteomic databases

PaxDbiP43368.
PeptideAtlasiP43368.
PRIDEiP43368.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397349.
KEGGissc:397349.

Organism-specific databases

CTDi825.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiP43368.
KOiK08573.

Enzyme and pathway databases

BRENDAi3.4.22.54. 6170.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029531. CAPN3.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF320. PTHR10183:SF320. 2 hits.
PfamiPF01067. Calpain_III. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN3_PIG
AccessioniPrimary (citable) accession number: P43368
Secondary accession number(s): O46596, Q28961
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 16, 2003
Last modified: November 30, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.