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Protein

Calpain-2 catalytic subunit

Gene

CAPN2

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.By similarity

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+By similarityNote: Binds 7 Ca2+ ions.By similarity

Enzyme regulationi

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi166Calcium 5; via carbonyl oxygenBy similarity1
Metal bindingi169Calcium 5By similarity1
Metal bindingi171Calcium 5; via carbonyl oxygenBy similarity1
Metal bindingi176Calcium 5By similarity1
Metal bindingi209Calcium 6By similarity1
Metal bindingi211Calcium 6By similarity1
Metal bindingi213Calcium 6; via carbonyl oxygenBy similarity1
Metal bindingi215Calcium 6; via carbonyl oxygenBy similarity1
Metal bindingi220Calcium 6By similarity1
Metal bindingi239Calcium 7By similarity1
Metal bindingi241Calcium 7By similarity1
Metal bindingi243Calcium 7; via carbonyl oxygenBy similarity1
Metal bindingi245Calcium 7; via carbonyl oxygenBy similarity1
Metal bindingi250Calcium 7By similarity1
Metal bindingi282Calcium 1By similarity1
Metal bindingi285Calcium 1By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi209 – 2201Add BLAST12
Calcium bindingi239 – 2502Add BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.53. 6170.

Protein family/group databases

MEROPSiC02.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-2 catalytic subunit (EC:3.4.22.53)
Alternative name(s):
Calcium-activated neutral proteinase 2
Short name:
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name:
M-calpain
Gene namesi
Name:CAPN2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4143.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000207703‹1 – 324Calpain-2 catalytic subunitAdd BLAST›324

Proteomic databases

PeptideAtlasiP43367.
PRIDEiP43367.

Expressioni

Tissue specificityi

Ubiquitous.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Interacts with CPEB3; this leads to cleavage of CPEB3.By similarity

Chemistry databases

BindingDBiP43367.

Structurei

3D structure databases

ProteinModelPortaliP43367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini190 – 224EF-hand 1PROSITE-ProRule annotationAdd BLAST35
Domaini226 – 261EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni‹1 – 138Domain IIIAdd BLAST›138
Regioni139 – 153LinkerAdd BLAST15
Regioni158 – 324Domain IVAdd BLAST167

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains at least 2 EF-hand domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG012645.
InParanoidiP43367.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00720. calpain_III. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P43367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
YPNTFWMNPQ YLIKLEEEDE DQEDGESGCT FLVGLIQKHR RRQRKMGEDM
60 70 80 90 100
HTIGFGIYEV PEELTGQTNI HLSKNFFLTH RARERSDTFI NLREVLNRFK
110 120 130 140 150
LPPGEYILVP STFEPNKDGD FCIRVFSEKK ADYQVVDDEI EADLEENDAS
160 170 180 190 200
EDDIDDGFRR LFAQLAGEDA EISAFELQTI LRRVLAKRQD IKSDGFSIET
210 220 230 240 250
CRIMVDMLDS DGSAKLGLKE FYILWTKIQK YQKIYREIDV DRSGTMNSYE
260 270 280 290 300
MRKALEEAGF KLPCQLHQVI VARFADDQLI IDFDNFVRCL VRLETLFRIS
310 320
KQLDSENTGT IELDLISWLC FSVL
Length:324
Mass (Da):37,809
Last modified:November 1, 1997 - v2
Checksum:i3929553239E123CF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Sequence conflicti202R → K in AAB17381 (PubMed:9728040).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01181 mRNA. Translation: AAC48401.1.
U71320 mRNA. Translation: AAB17381.1.
UniGeneiSsc.49836.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01181 mRNA. Translation: AAC48401.1.
U71320 mRNA. Translation: AAB17381.1.
UniGeneiSsc.49836.

3D structure databases

ProteinModelPortaliP43367.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP43367.
ChEMBLiCHEMBL4143.

Protein family/group databases

MEROPSiC02.002.

Proteomic databases

PeptideAtlasiP43367.
PRIDEiP43367.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG012645.
InParanoidiP43367.

Enzyme and pathway databases

BRENDAi3.4.22.53. 6170.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00720. calpain_III. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN2_PIG
AccessioniPrimary (citable) accession number: P43367
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.