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Protein

Calpain-2 catalytic subunit

Gene

CAPN2

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (By similarity).By similarity

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+By similarityNote: Binds 7 Ca2+ ions.By similarity

Enzyme regulationi

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi166 – 1661Calcium 5; via carbonyl oxygenBy similarity
Metal bindingi169 – 1691Calcium 5By similarity
Metal bindingi171 – 1711Calcium 5; via carbonyl oxygenBy similarity
Metal bindingi176 – 1761Calcium 5By similarity
Metal bindingi209 – 2091Calcium 6By similarity
Metal bindingi211 – 2111Calcium 6By similarity
Metal bindingi213 – 2131Calcium 6; via carbonyl oxygenBy similarity
Metal bindingi215 – 2151Calcium 6; via carbonyl oxygenBy similarity
Metal bindingi220 – 2201Calcium 6By similarity
Metal bindingi239 – 2391Calcium 7By similarity
Metal bindingi241 – 2411Calcium 7By similarity
Metal bindingi243 – 2431Calcium 7; via carbonyl oxygenBy similarity
Metal bindingi245 – 2451Calcium 7; via carbonyl oxygenBy similarity
Metal bindingi250 – 2501Calcium 7By similarity
Metal bindingi282 – 2821Calcium 1By similarity
Metal bindingi285 – 2851Calcium 1By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi209 – 220121Add
BLAST
Calcium bindingi239 – 250122Add
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.53. 6170.

Protein family/group databases

MEROPSiC02.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-2 catalytic subunit (EC:3.4.22.53)
Alternative name(s):
Calcium-activated neutral proteinase 2
Short name:
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name:
M-calpain
Gene namesi
Name:CAPN2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227 Componenti: Unplaced

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 324›324Calpain-2 catalytic subunitPRO_0000207703Add
BLAST

Proteomic databases

PRIDEiP43367.

Expressioni

Tissue specificityi

Ubiquitous.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Structurei

3D structure databases

ProteinModelPortaliP43367.
SMRiP43367. Positions 1-324.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini190 – 22435EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini226 – 26136EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni‹1 – 138›138Domain IIIAdd
BLAST
Regioni139 – 15315LinkerAdd
BLAST
Regioni158 – 324167Domain IVAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains at least 2 EF-hand domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG012645.
InParanoidiP43367.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
[Graphical view]
SMARTiSM00720. calpain_III. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P43367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
YPNTFWMNPQ YLIKLEEEDE DQEDGESGCT FLVGLIQKHR RRQRKMGEDM
60 70 80 90 100
HTIGFGIYEV PEELTGQTNI HLSKNFFLTH RARERSDTFI NLREVLNRFK
110 120 130 140 150
LPPGEYILVP STFEPNKDGD FCIRVFSEKK ADYQVVDDEI EADLEENDAS
160 170 180 190 200
EDDIDDGFRR LFAQLAGEDA EISAFELQTI LRRVLAKRQD IKSDGFSIET
210 220 230 240 250
CRIMVDMLDS DGSAKLGLKE FYILWTKIQK YQKIYREIDV DRSGTMNSYE
260 270 280 290 300
MRKALEEAGF KLPCQLHQVI VARFADDQLI IDFDNFVRCL VRLETLFRIS
310 320
KQLDSENTGT IELDLISWLC FSVL
Length:324
Mass (Da):37,809
Last modified:November 1, 1997 - v2
Checksum:i3929553239E123CF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Sequence conflicti202 – 2021R → K in AAB17381 (PubMed:9728040).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01181 mRNA. Translation: AAC48401.1.
U71320 mRNA. Translation: AAB17381.1.
UniGeneiSsc.49836.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01181 mRNA. Translation: AAC48401.1.
U71320 mRNA. Translation: AAB17381.1.
UniGeneiSsc.49836.

3D structure databases

ProteinModelPortaliP43367.
SMRiP43367. Positions 1-324.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiP43367.
ChEMBLiCHEMBL4143.

Protein family/group databases

MEROPSiC02.002.

Proteomic databases

PRIDEiP43367.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG012645.
InParanoidiP43367.

Enzyme and pathway databases

BRENDAi3.4.22.53. 6170.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
[Graphical view]
SMARTiSM00720. calpain_III. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning the partial cDNAs of mu-calpain and m-calpain from porcine skeletal muscle."
    Sun W., Ji S.Q., Ebert P.J., Bidwell C.A., Hancock D.L.
    Biochimie 75:931-936(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-209.
    Tissue: Skeletal muscle.
  2. "Hypoxia-specific upregulation of calpain activity and gene expression in pulmonary artery endothelial cells."
    Zhang J.L., Patel J.M., Block E.R.
    Am. J. Physiol. 275:L461-L468(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-324.
    Tissue: Pulmonary artery.

Entry informationi

Entry nameiCAN2_PIG
AccessioniPrimary (citable) accession number: P43367
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: July 22, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.