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P43367

- CAN2_PIG

UniProt

P43367 - CAN2_PIG

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Protein
Calpain-2 catalytic subunit
Gene
CAPN2
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' By similarity.

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Binds 7 calcium ions By similarity.

Enzyme regulationi

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi166 – 1661Calcium 5; via carbonyl oxygen By similarity
Metal bindingi169 – 1691Calcium 5 By similarity
Metal bindingi171 – 1711Calcium 5; via carbonyl oxygen By similarity
Metal bindingi176 – 1761Calcium 5 By similarity
Metal bindingi209 – 2091Calcium 6 By similarity
Metal bindingi211 – 2111Calcium 6 By similarity
Metal bindingi213 – 2131Calcium 6; via carbonyl oxygen By similarity
Metal bindingi215 – 2151Calcium 6; via carbonyl oxygen By similarity
Metal bindingi220 – 2201Calcium 6 By similarity
Metal bindingi239 – 2391Calcium 7 By similarity
Metal bindingi241 – 2411Calcium 7 By similarity
Metal bindingi243 – 2431Calcium 7; via carbonyl oxygen By similarity
Metal bindingi245 – 2451Calcium 7; via carbonyl oxygen By similarity
Metal bindingi250 – 2501Calcium 7 By similarity
Metal bindingi282 – 2821Calcium 1 By similarity
Metal bindingi285 – 2851Calcium 1 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi209 – 220121
Add
BLAST
Calcium bindingi239 – 250122
Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

  1. cellular response to amino acid stimulus Source: UniProtKB
  2. proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.53. 6170.

Protein family/group databases

MEROPSiC02.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-2 catalytic subunit (EC:3.4.22.53)
Alternative name(s):
Calcium-activated neutral proteinase 2
Short name:
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name:
M-calpain
Gene namesi
Name:CAPN2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. dendrite Source: UniProtKB
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 324›324Calpain-2 catalytic subunit
PRO_0000207703Add
BLAST

Proteomic databases

PRIDEiP43367.

Expressioni

Tissue specificityi

Ubiquitous.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Structurei

3D structure databases

ProteinModelPortaliP43367.
SMRiP43367. Positions 1-324.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini190 – 22435EF-hand 1
Add
BLAST
Domaini226 – 26136EF-hand 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni‹1 – 138›138Domain III
Add
BLAST
Regioni139 – 15315Linker
Add
BLAST
Regioni158 – 324167Domain IV
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.
Contains at least 2 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG012645.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
[Graphical view]
SMARTiSM00720. calpain_III. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P43367-1 [UniParc]FASTAAdd to Basket

« Hide

YPNTFWMNPQ YLIKLEEEDE DQEDGESGCT FLVGLIQKHR RRQRKMGEDM    50
HTIGFGIYEV PEELTGQTNI HLSKNFFLTH RARERSDTFI NLREVLNRFK 100
LPPGEYILVP STFEPNKDGD FCIRVFSEKK ADYQVVDDEI EADLEENDAS 150
EDDIDDGFRR LFAQLAGEDA EISAFELQTI LRRVLAKRQD IKSDGFSIET 200
CRIMVDMLDS DGSAKLGLKE FYILWTKIQK YQKIYREIDV DRSGTMNSYE 250
MRKALEEAGF KLPCQLHQVI VARFADDQLI IDFDNFVRCL VRLETLFRIS 300
KQLDSENTGT IELDLISWLC FSVL 324
Length:324
Mass (Da):37,809
Last modified:November 1, 1997 - v2
Checksum:i3929553239E123CF
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti202 – 2021R → K in AAB17381. 1 Publication

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U01181 mRNA. Translation: AAC48401.1.
U71320 mRNA. Translation: AAB17381.1.
UniGeneiSsc.49836.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U01181 mRNA. Translation: AAC48401.1 .
U71320 mRNA. Translation: AAB17381.1 .
UniGenei Ssc.49836.

3D structure databases

ProteinModelPortali P43367.
SMRi P43367. Positions 1-324.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P43367.
ChEMBLi CHEMBL4143.

Protein family/group databases

MEROPSi C02.002.

Proteomic databases

PRIDEi P43367.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG012645.

Enzyme and pathway databases

BRENDAi 3.4.22.53. 6170.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view ]
PANTHERi PTHR10183:SF268. PTHR10183:SF268. 1 hit.
Pfami PF01067. Calpain_III. 1 hit.
[Graphical view ]
SMARTi SM00720. calpain_III. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning the partial cDNAs of mu-calpain and m-calpain from porcine skeletal muscle."
    Sun W., Ji S.Q., Ebert P.J., Bidwell C.A., Hancock D.L.
    Biochimie 75:931-936(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-209.
    Tissue: Skeletal muscle.
  2. "Hypoxia-specific upregulation of calpain activity and gene expression in pulmonary artery endothelial cells."
    Zhang J.L., Patel J.M., Block E.R.
    Am. J. Physiol. 275:L461-L468(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-324.
    Tissue: Pulmonary artery.

Entry informationi

Entry nameiCAN2_PIG
AccessioniPrimary (citable) accession number: P43367
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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