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P43367

- CAN2_PIG

UniProt

P43367 - CAN2_PIG

Protein

Calpain-2 catalytic subunit

Gene

CAPN2

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' By similarity.By similarity

    Catalytic activityi

    Broad endopeptidase specificity.

    Cofactori

    Binds 7 calcium ions.By similarity

    Enzyme regulationi

    Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi166 – 1661Calcium 5; via carbonyl oxygenBy similarity
    Metal bindingi169 – 1691Calcium 5By similarity
    Metal bindingi171 – 1711Calcium 5; via carbonyl oxygenBy similarity
    Metal bindingi176 – 1761Calcium 5By similarity
    Metal bindingi209 – 2091Calcium 6By similarity
    Metal bindingi211 – 2111Calcium 6By similarity
    Metal bindingi213 – 2131Calcium 6; via carbonyl oxygenBy similarity
    Metal bindingi215 – 2151Calcium 6; via carbonyl oxygenBy similarity
    Metal bindingi220 – 2201Calcium 6By similarity
    Metal bindingi239 – 2391Calcium 7By similarity
    Metal bindingi241 – 2411Calcium 7By similarity
    Metal bindingi243 – 2431Calcium 7; via carbonyl oxygenBy similarity
    Metal bindingi245 – 2451Calcium 7; via carbonyl oxygenBy similarity
    Metal bindingi250 – 2501Calcium 7By similarity
    Metal bindingi282 – 2821Calcium 1By similarity
    Metal bindingi285 – 2851Calcium 1By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi209 – 220121Add
    BLAST
    Calcium bindingi239 – 250122Add
    BLAST

    GO - Molecular functioni

    1. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
    2. calcium ion binding Source: InterPro

    GO - Biological processi

    1. cellular response to amino acid stimulus Source: UniProtKB
    2. proteolysis Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.4.22.53. 6170.

    Protein family/group databases

    MEROPSiC02.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calpain-2 catalytic subunit (EC:3.4.22.53)
    Alternative name(s):
    Calcium-activated neutral proteinase 2
    Short name:
    CANP 2
    Calpain M-type
    Calpain-2 large subunit
    Millimolar-calpain
    Short name:
    M-calpain
    Gene namesi
    Name:CAPN2
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity
    Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. dendrite Source: UniProtKB
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 324›324Calpain-2 catalytic subunitPRO_0000207703Add
    BLAST

    Proteomic databases

    PRIDEiP43367.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Interactioni

    Subunit structurei

    Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

    Structurei

    3D structure databases

    ProteinModelPortaliP43367.
    SMRiP43367. Positions 1-324.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini190 – 22435EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini226 – 26136EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni‹1 – 138›138Domain IIIAdd
    BLAST
    Regioni139 – 15315LinkerAdd
    BLAST
    Regioni158 – 324167Domain IVAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C2 family.Curated
    Contains at least 2 EF-hand domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOVERGENiHBG012645.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029539. CAPN2.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
    PfamiPF01067. Calpain_III. 1 hit.
    [Graphical view]
    SMARTiSM00720. calpain_III. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view]
    SUPFAMiSSF49758. SSF49758. 1 hit.
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    P43367-1 [UniParc]FASTAAdd to Basket

    « Hide

    YPNTFWMNPQ YLIKLEEEDE DQEDGESGCT FLVGLIQKHR RRQRKMGEDM    50
    HTIGFGIYEV PEELTGQTNI HLSKNFFLTH RARERSDTFI NLREVLNRFK 100
    LPPGEYILVP STFEPNKDGD FCIRVFSEKK ADYQVVDDEI EADLEENDAS 150
    EDDIDDGFRR LFAQLAGEDA EISAFELQTI LRRVLAKRQD IKSDGFSIET 200
    CRIMVDMLDS DGSAKLGLKE FYILWTKIQK YQKIYREIDV DRSGTMNSYE 250
    MRKALEEAGF KLPCQLHQVI VARFADDQLI IDFDNFVRCL VRLETLFRIS 300
    KQLDSENTGT IELDLISWLC FSVL 324
    Length:324
    Mass (Da):37,809
    Last modified:November 1, 1997 - v2
    Checksum:i3929553239E123CF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Sequence conflicti202 – 2021R → K in AAB17381. (PubMed:9728040)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U01181 mRNA. Translation: AAC48401.1.
    U71320 mRNA. Translation: AAB17381.1.
    UniGeneiSsc.49836.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U01181 mRNA. Translation: AAC48401.1 .
    U71320 mRNA. Translation: AAB17381.1 .
    UniGenei Ssc.49836.

    3D structure databases

    ProteinModelPortali P43367.
    SMRi P43367. Positions 1-324.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P43367.
    ChEMBLi CHEMBL4143.

    Protein family/group databases

    MEROPSi C02.002.

    Proteomic databases

    PRIDEi P43367.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG012645.

    Enzyme and pathway databases

    BRENDAi 3.4.22.53. 6170.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029539. CAPN2.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    PANTHERi PTHR10183:SF268. PTHR10183:SF268. 1 hit.
    Pfami PF01067. Calpain_III. 1 hit.
    [Graphical view ]
    SMARTi SM00720. calpain_III. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49758. SSF49758. 1 hit.
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning the partial cDNAs of mu-calpain and m-calpain from porcine skeletal muscle."
      Sun W., Ji S.Q., Ebert P.J., Bidwell C.A., Hancock D.L.
      Biochimie 75:931-936(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-209.
      Tissue: Skeletal muscle.
    2. "Hypoxia-specific upregulation of calpain activity and gene expression in pulmonary artery endothelial cells."
      Zhang J.L., Patel J.M., Block E.R.
      Am. J. Physiol. 275:L461-L468(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-324.
      Tissue: Pulmonary artery.

    Entry informationi

    Entry nameiCAN2_PIG
    AccessioniPrimary (citable) accession number: P43367
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3