Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P43360 (MAGA6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Melanoma-associated antigen 6
Alternative name(s):
Cancer/testis antigen 1.6
Short name=CT1.6
MAGE-6 antigen
MAGE3B antigen
Gene names
Name:MAGEA6
Synonyms:MAGE6
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Ref.7 Ref.8

Subunit structure

Interacts with TRIM28. Ref.8

Tissue specificity

Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes.

Sequence similarities

Contains 1 MAGE domain.

Caution

In vitro experiments by Ref.7 measuring cell viability in melanoma cell lines used siRNA specific for MAGEA3 and MAGEA6.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Coding sequence diversityPolymorphism
   Molecular functionTumor antigen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TRIM28Q132632EBI-1045155,EBI-78139

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314Melanoma-associated antigen 6
PRO_0000156706

Regions

Domain109 – 308200MAGE
Compositional bias40 – 434Poly-Ser

Natural variations

Natural variant1521S → I. Ref.6 Ref.9
Corresponds to variant rs7056365 [ dbSNP | Ensembl ].
VAR_053493

Sequences

Sequence LengthMass (Da)Tools
P43360 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 29B83C7FA6E50263

FASTA31434,891
        10         20         30         40         50         60 
MPLEQRSQHC KPEEGLEARG EALGLVGAQA PATEEQEAAS SSSTLVEVTL GEVPAAESPD 

        70         80         90        100        110        120 
PPQSPQGASS LPTTMNYPLW SQSYEDSSNQ EEEGPSTFPD LESEFQAALS RKVAKLVHFL 

       130        140        150        160        170        180 
LLKYRAREPV TKAEMLGSVV GNWQYFFPVI FSKASDSLQL VFGIELMEVD PIGHVYIFAT 

       190        200        210        220        230        240 
CLGLSYDGLL GDNQIMPKTG FLIIILAIIA KEGDCAPEEK IWEELSVLEV FEGREDSIFG 

       250        260        270        280        290        300 
DPKKLLTQYF VQENYLEYRQ VPGSDPACYE FLWGPRALIE TSYVKVLHHM VKISGGPRIS 

       310 
YPLLHEWALR EGEE 

« Hide

References

« Hide 'large scale' references
[1]"Structure, chromosomal localization, and expression of 12 genes of the MAGE family."
De Plaen E., Arden K., Traversari C., Gaforio J.J., Szikora J.-P., De Smet C., Brasseur F., van der Bruggen P., Lethe B.G., Lurquin C., Brasseur R., Chomez P., de Backer O., Cavenee W., Boon T.
Immunogenetics 40:360-369(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cloning and analysis of MAGE-1-related genes."
Ding M., Beck R.J., Keller C.J., Fenton R.G.
Biochem. Biophys. Res. Commun. 202:549-555(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Skin.
[3]"Sequence analysis of the MAGE gene family encoding human tumor-rejection antigens."
Imai Y., Shichijo S., Yamada A., Katayama T., Yano H., Itoh K.
Gene 160:287-290(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The isolation and characterization of melanoma antigen 6 (MAGE-6)."
Mastutik G., Putra S.T., Soetjipto S., Kusumobroto H.O., Hardjowijoto S., Lunardi J.H., Puspaningsih N.N.T.
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Esophageal carcinoma.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-152.
Tissue: Brain and Testis.
[7]"MAGE-A, mMage-b, and MAGE-C proteins form complexes with KAP1 and suppress p53-dependent apoptosis in MAGE-positive cell lines."
Yang B., O'Herrin S.M., Wu J., Reagan-Shaw S., Ma Y., Bhat K.M., Gravekamp C., Setaluri V., Peters N., Hoffmann F.M., Peng H., Ivanov A.V., Simpson A.J., Longley B.J.
Cancer Res. 67:9954-9962(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases."
Doyle J.M., Gao J., Wang J., Yang M., Potts P.R.
Mol. Cell 39:963-974(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TRIM28.
[9]"Terminal osseous dysplasia is caused by a single recurrent mutation in the FLNA gene."
Sun Y., Almomani R., Aten E., Celli J., van der Heijden J., Venselaar H., Robertson S.P., Baroncini A., Franco B., Basel-Vanagaite L., Horii E., Drut R., Ariyurek Y., den Dunnen J.T., Breuning M.H.
Am. J. Hum. Genet. 87:146-153(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT ILE-152.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U10691 Genomic DNA. Translation: AAA68875.1.
U10339 mRNA. Translation: AAA19006.1.
D32076 mRNA. Translation: BAA06842.1.
EU161101 mRNA. Translation: ABW06860.1.
BX640600 mRNA. Translation: CAE45706.1.
BC041599 mRNA. Translation: AAH41599.1.
BC067731 mRNA. Translation: AAH67731.1.
PIRJC2360.
RefSeqNP_005354.1. NM_005363.2.
NP_787064.1. NM_175868.1.
UniGeneHs.441113.

3D structure databases

ProteinModelPortalP43360.
SMRP43360. Positions 100-311.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110279. 14 interactions.
IntActP43360. 6 interactions.
STRING9606.ENSP00000329199.

PTM databases

PhosphoSiteP43360.

Polymorphism databases

DMDM1170860.

Proteomic databases

PaxDbP43360.
PRIDEP43360.

Protocols and materials databases

DNASU4105.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000329342; ENSP00000329199; ENSG00000197172.
ENST00000596509; ENSP00000472505; ENSG00000269114.
GeneID4105.
KEGGhsa:4105.
UCSCuc004ffq.1. human.

Organism-specific databases

CTD4105.
GeneCardsGC0XP151867.
HGNCHGNC:6804. MAGEA6.
MIM300176. gene.
neXtProtNX_P43360.
PharmGKBPA30550.
GenAtlasSearch...

Phylogenomic databases

eggNOGKOG4562.
HOGENOMHOG000231161.
HOVERGENHBG006315.
InParanoidP43360.
OMACIRAANA.
PhylomeDBP43360.
TreeFamTF328505.

Gene expression databases

BgeeP43360.
CleanExHS_MAGEA6.
GenevestigatorP43360.

Family and domain databases

InterProIPR002190. MAGE.
IPR021072. Melanoma_ass_antigen_N.
[Graphical view]
PANTHERPTHR11736. PTHR11736. 1 hit.
PfamPF01454. MAGE. 1 hit.
PF12440. MAGE_N. 1 hit.
[Graphical view]
PROSITEPS50838. MAGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi4105.
NextBio16110.
PROP43360.
SOURCESearch...

Entry information

Entry nameMAGA6_HUMAN
AccessionPrimary (citable) accession number: P43360
Secondary accession number(s): A8IF93, Q6NW44
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM