P43360 (MAGA6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Melanoma-associated antigen 6

Alternative name(s):
Cancer/testis antigen 1.6
Short name=CT1.6
MAGE-6 antigen
MAGE3B antigen

Gene names

Name:	MAGEA6
Synonyms:	MAGE6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Ref.7 Ref.8
Subunit structure	Interacts with TRIM28. Ref.8
Tissue specificity	Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes.
Sequence similarities	Contains 1 MAGE domain.
Caution	In vitro experiments by Ref.7 measuring cell viability in melanoma cell lines used siRNA specific for MAGEA3 and MAGEA6.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Polymorphism
Molecular function	Tumor antigen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRIM28	Q13263	2	EBI-1045155,EBI-78139

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Melanoma-associated antigen 6		PRO_0000156706
Regions
	Domain	109 – 308	200	MAGE
	Compositional bias	40 – 43	4	Poly-Ser
Natural variations
	Natural variant	152	1	S → I. Ref.6 Ref.9 Corresponds to variant rs7056365 [ dbSNP | Ensembl ].		VAR_053493

Sequences

	Sequence		Length	Mass (Da)
	P43360 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 29B83C7FA6E50263	FASTA	314	34,891
	10 20 30 40 50 60 MPLEQRSQHC KPEEGLEARG EALGLVGAQA PATEEQEAAS SSSTLVEVTL GEVPAAESPD 70 80 90 100 110 120 PPQSPQGASS LPTTMNYPLW SQSYEDSSNQ EEEGPSTFPD LESEFQAALS RKVAKLVHFL 130 140 150 160 170 180 LLKYRAREPV TKAEMLGSVV GNWQYFFPVI FSKASDSLQL VFGIELMEVD PIGHVYIFAT 190 200 210 220 230 240 CLGLSYDGLL GDNQIMPKTG FLIIILAIIA KEGDCAPEEK IWEELSVLEV FEGREDSIFG 250 260 270 280 290 300 DPKKLLTQYF VQENYLEYRQ VPGSDPACYE FLWGPRALIE TSYVKVLHHM VKISGGPRIS 310 YPLLHEWALR EGEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure, chromosomal localization, and expression of 12 genes of the MAGE family." De Plaen E., Arden K., Traversari C., Gaforio J.J., Szikora J.-P., De Smet C., Brasseur F., van der Bruggen P., Lethe B.G., Lurquin C., Brasseur R., Chomez P., de Backer O., Cavenee W., Boon T. Immunogenetics 40:360-369(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and analysis of MAGE-1-related genes." Ding M., Beck R.J., Keller C.J., Fenton R.G. Biochem. Biophys. Res. Commun. 202:549-555(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skin.
[3]	"Sequence analysis of the MAGE gene family encoding human tumor-rejection antigens." Imai Y., Shichijo S., Yamada A., Katayama T., Yano H., Itoh K. Gene 160:287-290(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The isolation and characterization of melanoma antigen 6 (MAGE-6)." Mastutik G., Putra S.T., Soetjipto S., Kusumobroto H.O., Hardjowijoto S., Lunardi J.H., Puspaningsih N.N.T. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Esophageal carcinoma.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-152. Tissue: Brain and Testis.
[7]	"MAGE-A, mMage-b, and MAGE-C proteins form complexes with KAP1 and suppress p53-dependent apoptosis in MAGE-positive cell lines." Yang B., O'Herrin S.M., Wu J., Reagan-Shaw S., Ma Y., Bhat K.M., Gravekamp C., Setaluri V., Peters N., Hoffmann F.M., Peng H., Ivanov A.V., Simpson A.J., Longley B.J. Cancer Res. 67:9954-9962(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases." Doyle J.M., Gao J., Wang J., Yang M., Potts P.R. Mol. Cell 39:963-974(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TRIM28.
[9]	"Terminal osseous dysplasia is caused by a single recurrent mutation in the FLNA gene." Sun Y., Almomani R., Aten E., Celli J., van der Heijden J., Venselaar H., Robertson S.P., Baroncini A., Franco B., Basel-Vanagaite L., Horii E., Drut R., Ariyurek Y., den Dunnen J.T., Breuning M.H. Am. J. Hum. Genet. 87:146-153(2010) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT ILE-152.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10691 Genomic DNA. Translation: AAA68875.1. U10339 mRNA. Translation: AAA19006.1. D32076 mRNA. Translation: BAA06842.1. EU161101 mRNA. Translation: ABW06860.1. BX640600 mRNA. Translation: CAE45706.1. BC041599 mRNA. Translation: AAH41599.1. BC067731 mRNA. Translation: AAH67731.1.
IPI	IPI00018068.
PIR	JC2360.
RefSeq	NP_005354.1. NM_005363.2. NP_787064.1. NM_175868.1.
UniGene	Hs.441113.
3D structure databases
ProteinModelPortal	P43360.
ModBase	Search...
Protein-protein interaction databases
IntAct	P43360. 4 interactions.
STRING	9606.ENSP00000329199.
PTM databases
PhosphoSite	P43360.
Polymorphism databases
DMDM	1170860.
Proteomic databases
PaxDb	P43360.
PRIDE	P43360.
Protocols and materials databases
DNASU	4105.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000329342; ENSP00000329199; ENSG00000197172. ENST00000596509; ENSP00000472505; ENSG00000269114.
GeneID	4105.
KEGG	hsa:4105.
UCSC	uc004ffq.1. human.
Organism-specific databases
CTD	4105.
GeneCards	GC0XP151867.
HGNC	HGNC:6804. MAGEA6.
MIM	300176. gene.
neXtProt	NX_P43360.
PharmGKB	PA30550.
GenAtlas	Search...
Phylogenomic databases
eggNOG	KOG4562.
HOGENOM	HOG000231161.
HOVERGEN	HBG006315.
InParanoid	P43360.
OMA	FDEREYS.
OrthoDB	EOG4TXBSK.
Gene expression databases
ArrayExpress	P43360.
Bgee	P43360.
CleanEx	HS_MAGEA6.
Genevestigator	P43360.
GermOnline	ENSG00000183305. Homo sapiens.
Family and domain databases
InterPro	IPR002190. MAGE. IPR021072. Melanoma_ass_antigen_N. [Graphical view]
PANTHER	PTHR11736. PTHR11736. 1 hit.
Pfam	PF01454. MAGE. 1 hit. PF12440. MAGE_N. 1 hit. [Graphical view]
PROSITE	PS50838. MAGE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	4105.
NextBio	16110.
SOURCE	Search...

Entry information

Entry name

MAGA6_HUMAN

Accession

Primary (citable) accession number: P43360
Secondary accession number(s): A8IF93, Q6NW44

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.