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Protein

Melanoma-associated antigen 6

Gene

MAGEA6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines.2 Publications

Keywords - Molecular functioni

Tumor antigen

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma-associated antigen 6
Alternative name(s):
Cancer/testis antigen 1.6
Short name:
CT1.6
MAGE-6 antigen
MAGE3B antigen
Gene namesi
Name:MAGEA6
Synonyms:MAGE6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome X

Organism-specific databases

HGNCiHGNC:6804. MAGEA6.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30550.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Melanoma-associated antigen 6PRO_0000156706Add
BLAST

Proteomic databases

MaxQBiP43360.
PaxDbiP43360.
PRIDEiP43360.

PTM databases

PhosphoSiteiP43360.

Expressioni

Tissue specificityi

Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes.

Gene expression databases

BgeeiP43360.
CleanExiHS_MAGEA6.
ExpressionAtlasiP43360. baseline.
GenevestigatoriP43360.

Interactioni

Subunit structurei

Interacts with TRIM28.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TRIM28Q132632EBI-1045155,EBI-78139

Protein-protein interaction databases

BioGridi110279. 49 interactions.
IntActiP43360. 6 interactions.
STRINGi9606.ENSP00000329199.

Structurei

3D structure databases

ProteinModelPortaliP43360.
SMRiP43360. Positions 100-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini109 – 308200MAGEPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi40 – 434Poly-Ser

Sequence similaritiesi

Contains 1 MAGE domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4562.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000231161.
HOVERGENiHBG006315.
InParanoidiP43360.
OMAiCIRAANA.
PhylomeDBiP43360.
TreeFamiTF328505.

Family and domain databases

InterProiIPR030097. MAGEA3/MAGEA6.
IPR021072. Melanoma_ass_antigen_N.
IPR002190. MHD_dom.
[Graphical view]
PANTHERiPTHR11736. PTHR11736. 1 hit.
PTHR11736:SF60. PTHR11736:SF60. 1 hit.
PfamiPF01454. MAGE. 1 hit.
PF12440. MAGE_N. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43360-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLEQRSQHC KPEEGLEARG EALGLVGAQA PATEEQEAAS SSSTLVEVTL
60 70 80 90 100
GEVPAAESPD PPQSPQGASS LPTTMNYPLW SQSYEDSSNQ EEEGPSTFPD
110 120 130 140 150
LESEFQAALS RKVAKLVHFL LLKYRAREPV TKAEMLGSVV GNWQYFFPVI
160 170 180 190 200
FSKASDSLQL VFGIELMEVD PIGHVYIFAT CLGLSYDGLL GDNQIMPKTG
210 220 230 240 250
FLIIILAIIA KEGDCAPEEK IWEELSVLEV FEGREDSIFG DPKKLLTQYF
260 270 280 290 300
VQENYLEYRQ VPGSDPACYE FLWGPRALIE TSYVKVLHHM VKISGGPRIS
310
YPLLHEWALR EGEE
Length:314
Mass (Da):34,891
Last modified:November 1, 1995 - v1
Checksum:i29B83C7FA6E50263
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti152 – 1521S → I.2 Publications
Corresponds to variant rs7056365 [ dbSNP | Ensembl ].
VAR_053493

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10691 Genomic DNA. Translation: AAA68875.1.
U10339 mRNA. Translation: AAA19006.1.
D32076 mRNA. Translation: BAA06842.1.
EU161101 mRNA. Translation: ABW06860.1.
BX640600 mRNA. Translation: CAE45706.1.
BC041599 mRNA. Translation: AAH41599.1.
BC067731 mRNA. Translation: AAH67731.1.
CCDSiCCDS76050.1.
PIRiJC2360.
RefSeqiNP_005354.1. NM_005363.2.
NP_787064.1. NM_175868.1.
UniGeneiHs.441113.

Genome annotation databases

EnsembliENST00000329342; ENSP00000329199; ENSG00000197172.
ENST00000616035; ENSP00000480637; ENSG00000197172.
GeneIDi4105.
KEGGihsa:4105.
UCSCiuc004ffq.1. human.

Polymorphism databases

DMDMi1170860.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10691 Genomic DNA. Translation: AAA68875.1.
U10339 mRNA. Translation: AAA19006.1.
D32076 mRNA. Translation: BAA06842.1.
EU161101 mRNA. Translation: ABW06860.1.
BX640600 mRNA. Translation: CAE45706.1.
BC041599 mRNA. Translation: AAH41599.1.
BC067731 mRNA. Translation: AAH67731.1.
CCDSiCCDS76050.1.
PIRiJC2360.
RefSeqiNP_005354.1. NM_005363.2.
NP_787064.1. NM_175868.1.
UniGeneiHs.441113.

3D structure databases

ProteinModelPortaliP43360.
SMRiP43360. Positions 100-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110279. 49 interactions.
IntActiP43360. 6 interactions.
STRINGi9606.ENSP00000329199.

PTM databases

PhosphoSiteiP43360.

Polymorphism databases

DMDMi1170860.

Proteomic databases

MaxQBiP43360.
PaxDbiP43360.
PRIDEiP43360.

Protocols and materials databases

DNASUi4105.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000329342; ENSP00000329199; ENSG00000197172.
ENST00000616035; ENSP00000480637; ENSG00000197172.
GeneIDi4105.
KEGGihsa:4105.
UCSCiuc004ffq.1. human.

Organism-specific databases

CTDi4105.
GeneCardsiGC0XP151867.
HGNCiHGNC:6804. MAGEA6.
MIMi300176. gene.
neXtProtiNX_P43360.
PharmGKBiPA30550.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4562.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000231161.
HOVERGENiHBG006315.
InParanoidiP43360.
OMAiCIRAANA.
PhylomeDBiP43360.
TreeFamiTF328505.

Miscellaneous databases

GenomeRNAii4105.
NextBioi16110.
PROiP43360.
SOURCEiSearch...

Gene expression databases

BgeeiP43360.
CleanExiHS_MAGEA6.
ExpressionAtlasiP43360. baseline.
GenevestigatoriP43360.

Family and domain databases

InterProiIPR030097. MAGEA3/MAGEA6.
IPR021072. Melanoma_ass_antigen_N.
IPR002190. MHD_dom.
[Graphical view]
PANTHERiPTHR11736. PTHR11736. 1 hit.
PTHR11736:SF60. PTHR11736:SF60. 1 hit.
PfamiPF01454. MAGE. 1 hit.
PF12440. MAGE_N. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skin.
  3. "Sequence analysis of the MAGE gene family encoding human tumor-rejection antigens."
    Imai Y., Shichijo S., Yamada A., Katayama T., Yano H., Itoh K.
    Gene 160:287-290(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The isolation and characterization of melanoma antigen 6 (MAGE-6)."
    Mastutik G., Putra S.T., Soetjipto S., Kusumobroto H.O., Hardjowijoto S., Lunardi J.H., Puspaningsih N.N.T.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Esophageal carcinoma.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-152.
    Tissue: Brain and Testis.
  7. "MAGE-A, mMage-b, and MAGE-C proteins form complexes with KAP1 and suppress p53-dependent apoptosis in MAGE-positive cell lines."
    Yang B., O'Herrin S.M., Wu J., Reagan-Shaw S., Ma Y., Bhat K.M., Gravekamp C., Setaluri V., Peters N., Hoffmann F.M., Peng H., Ivanov A.V., Simpson A.J., Longley B.J.
    Cancer Res. 67:9954-9962(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases."
    Doyle J.M., Gao J., Wang J., Yang M., Potts P.R.
    Mol. Cell 39:963-974(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TRIM28.
  9. Cited for: VARIANT ILE-152.

Entry informationi

Entry nameiMAGA6_HUMAN
AccessioniPrimary (citable) accession number: P43360
Secondary accession number(s): A8IF93, Q6NW44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 4, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

In vitro experiments by PubMed:17942928 measuring cell viability in melanoma cell lines used siRNA specific for MAGEA3 and MAGEA6.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.