P43358 (MAGA4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Melanoma-associated antigen 4

Alternative name(s):
Cancer/testis antigen 1.4
Short name=CT1.4
MAGE-4 antigen
MAGE-41 antigen
MAGE-X2 antigen

Gene names

Name:	MAGEA4
Synonyms:	MAGE4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
Tissue specificity	Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
Sequence similarities	Contains 1 MAGE domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Molecular function	Tumor antigen
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PSMD10	O75832	5	EBI-743122,EBI-752185
ZBTB17	Q13105	5	EBI-743122,EBI-372156

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 317

317

Melanoma-associated antigen 4

PRO_0000156704

Regions

Domain

110 – 309

200

MAGE

Compositional bias

41 – 44

4

Poly-Ser

Natural variations

Natural variant

153

1

G → D in a breast cancer sample; somatic mutation. Ref.8

VAR_036582

Natural variant

173

1

A → T. Ref.1 Ref.2 Ref.3 Ref.5
Corresponds to variant rs1047251 [ dbSNP | Ensembl ].

VAR_004284

Experimental info

Sequence conflict

3

1

S → L in BAA06841. Ref.3

Sequence conflict

48

1

P → L in BAA06841. Ref.3

Sequence conflict

53

1

E → K in BAA06841. Ref.3

Sequence conflict

61

1

G → D in BAA06841. Ref.3

Sequence conflict

95 – 96

2

GP → EA in BAA06841. Ref.3

Sequence conflict

307

1

E → Q in AAA19007. Ref.2

Secondary structure

1

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317

Helix Strand Turn

Details...

Helix

102 – 126

25

Helix

133 – 140

8

Helix

145 – 147

3

Helix

148 – 151

4

Helix

155 – 162

8

Beta strand

166 – 169

4

Beta strand

176 – 180

5

Helix

203 – 206

4

Turn

210 – 212

3

Helix

219 – 225

7

Helix

243 – 248

6

Helix

250 – 253

4

Beta strand

258 – 260

3

Beta strand

271 – 273

3

Helix

277 – 281

5

Helix

284 – 295

12

Helix

305 – 309

5

Sequences

	Sequence		Length	Mass (Da)
	P43358 [UniParc]. Last modified February 8, 2011. Version 2. Checksum: C6AA2EE306963E7D	FASTA	317	34,899
	10 20 30 40 50 60 MSSEQKSQHC KPEEGVEAQE EALGLVGAQA PTTEEQEAAV SSSSPLVPGT LEEVPAAESA 70 80 90 100 110 120 GPPQSPQGAS ALPTTISFTC WRQPNEGSSS QEEEGPSTSP DAESLFREAL SNKVDELAHF 130 140 150 160 170 180 LLRKYRAKEL VTKAEMLERV IKNYKRCFPV IFGKASESLK MIFGIDVKEV DPASNTYTLV 190 200 210 220 230 240 TCLGLSYDGL LGNNQIFPKT GLLIIVLGTI AMEGDSASEE EIWEELGVMG VYDGREHTVY 250 260 270 280 290 300 GEPRKLLTQD WVQENYLEYR QVPGSNPARY EFLWGPRALA ETSYVKVLEH VVRVNARVRI 310 AYPSLREAAL LEEEEGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure, chromosomal localization, and expression of 12 genes of the MAGE family." De Plaen E., Arden K., Traversari C., Gaforio J.J., Szikora J.-P., De Smet C., Brasseur F., van der Bruggen P., Lethe B.G., Lurquin C., Brasseur R., Chomez P., de Backer O., Cavenee W., Boon T. Immunogenetics 40:360-369(1994) [PubMed: 7927540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-173. Tissue: Blood.
[2]	"Cloning and analysis of MAGE-1-related genes." Ding M., Beck R.J., Keller C.J., Fenton R.G. Biochem. Biophys. Res. Commun. 202:549-555(1994) [PubMed: 8037761] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-173. Tissue: Skin.
[3]	"Sequence analysis of the MAGE gene family encoding human tumor-rejection antigens." Imai Y., Shichijo S., Yamada A., Katayama T., Yano H., Itoh K. Gene 160:287-290(1995) [PubMed: 7642112] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-173.
[4]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed: 15772651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-173. Tissue: Duodenum.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"High-resolution structure of HLA-A*0201 in complex with a tumour-specific antigenic peptide encoded by the MAGE-A4 gene." Hillig R.C., Coulie P.G., Stroobant V., Saenger W., Ziegler A., Hulsmeyer M. J. Mol. Biol. 310:1167-1176(2001) [PubMed: 11502003] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 230-239.
[8]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ASP-153.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U10687 Genomic DNA. Translation: AAA68871.1.
U10688 Genomic DNA. Translation: AAA68872.1.
U10340 mRNA. Translation: AAA19007.1.
D32075 mRNA. Translation: BAA06841.1.
D32077 mRNA. Translation: BAA06843.1.
AF274855 Genomic DNA. No translation available.
BC017723 mRNA. Translation: AAH17723.1.

IPI

IPI00910524.

PIR

I38661.

RefSeq

NP_001011548.1. NM_001011548.1.
NP_001011549.1. NM_001011549.1.
NP_001011550.1. NM_001011550.1.
NP_002353.3. NM_002362.4.

UniGene

Hs.37107.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1I4F	X-ray	1.40	C	230-239	[»]
2WA0	X-ray	2.30	A	101-317	[»]

ProteinModelPortal

P43358.

SMR

P43358. Positions 99-312.

ModBase

Search...

Protein-protein interaction databases

IntAct

P43358. 5 interactions.

MINT

MINT-254770.

STRING

P43358.

PTM databases

PhosphoSite

P43358.

Polymorphism databases

DMDM

1170858.

Proteomic databases

PRIDE

P43358.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000276344; ENSP00000276344; ENSG00000147381.
ENST00000360243; ENSP00000353379; ENSG00000147381.
ENST00000370335; ENSP00000359360; ENSG00000147381.
ENST00000370337; ENSP00000359362; ENSG00000147381.
ENST00000370340; ENSP00000359365; ENSG00000147381.
ENST00000393920; ENSP00000377497; ENSG00000147381.
ENST00000393921; ENSP00000377498; ENSG00000147381.
ENST00000427663; ENSP00000392941; ENSG00000147381.

GeneID

4103.

KEGG

hsa:4103.

Organism-specific databases

CTD

4103.

GeneCards

GC0XP151080.

HGNC

HGNC:6802. MAGEA4.

HPA

CAB040535.
HPA021942.

MIM

300175. gene.

neXtProt

NX_P43358.

PharmGKB

PA30548.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG12188.

HOVERGEN

HBG006315.

InParanoid

P43358.

PhylomeDB

P43358.

Gene expression databases

ArrayExpress

P43358.

Bgee

P43358.

CleanEx

HS_MAGEA4.

Genevestigator

P43358.

GermOnline

ENSG00000147381. Homo sapiens.

Family and domain databases

InterPro

IPR002190. MAGE.
IPR021072. Melanoma_ass_antigen_N.
[Graphical view]

PANTHER

PTHR11736. MAGE. 1 hit.

Pfam

PF01454. MAGE. 1 hit.
PF12440. MAGE_N. 1 hit.
[Graphical view]

PROSITE

PS50838. MAGE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

SOURCE

Search...

Entry information

Entry name

MAGA4_HUMAN

Accession

Primary (citable) accession number: P43358
Secondary accession number(s): Q14798

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	February 8, 2011
Last modified:	January 25, 2012
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.