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Protein

Melanoma-associated antigen 3

Gene

MAGEA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.1 Publication

GO - Molecular functioni

  • caspase binding Source: ParkinsonsUK-UCL

GO - Biological processi

  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: ParkinsonsUK-UCL
  • negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
  • negative regulation of protein processing Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Tumor antigen

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:G66-33124-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma-associated antigen 3
Alternative name(s):
Antigen MZ2-D
Cancer/testis antigen 1.3
Short name:
CT1.3
MAGE-3 antigen
Gene namesi
Name:MAGEA3
Synonyms:MAGE3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:6801. MAGEA3.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: ParkinsonsUK-UCL
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi170D → A: Abolishes HLA-A1 binding. 1 Publication1
Mutagenesisi176Y → A: Abolishes HLA-A1 binding. 1 Publication1

Organism-specific databases

DisGeNETi4102.
OpenTargetsiENSG00000221867.
PharmGKBiPA30547.

Polymorphism and mutation databases

BioMutaiMAGEA3.
DMDMi1170857.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001567031 – 314Melanoma-associated antigen 3Add BLAST314

Proteomic databases

MaxQBiP43357.
PaxDbiP43357.
PeptideAtlasiP43357.
PRIDEiP43357.

PTM databases

iPTMnetiP43357.
PhosphoSitePlusiP43357.

Expressioni

Tissue specificityi

Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, Leukemias and lymphomas.

Gene expression databases

BgeeiENSG00000221867.
CleanExiHS_MAGEA3.
ExpressionAtlasiP43357. baseline and differential.
GenevisibleiP43357. HS.

Interactioni

Subunit structurei

Interacts with TRIM28.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TRIM28Q132633EBI-5651459,EBI-78139

GO - Molecular functioni

  • caspase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi110276. 5 interactors.
DIPiDIP-44886N.
IntActiP43357. 14 interactors.
MINTiMINT-1537705.
STRINGi9606.ENSP00000359301.

Structurei

Secondary structure

1314
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi104 – 126Combined sources23
Helixi132 – 138Combined sources7
Helixi141 – 146Combined sources6
Helixi147 – 162Combined sources16
Beta strandi164 – 170Combined sources7
Turni171 – 174Combined sources4
Beta strandi175 – 180Combined sources6
Helixi181 – 183Combined sources3
Helixi199 – 212Combined sources14
Helixi218 – 225Combined sources8
Helixi229 – 231Combined sources3
Helixi242 – 252Combined sources11
Beta strandi255 – 260Combined sources6
Beta strandi269 – 273Combined sources5
Helixi275 – 280Combined sources6
Helixi283 – 292Combined sources10
Turni293 – 295Combined sources3
Helixi304 – 306Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QEWX-ray2.20C271-279[»]
4V0PX-ray2.07A104-314[»]
5BRZX-ray2.62C168-176[»]
ProteinModelPortaliP43357.
SMRiP43357.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43357.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 308MAGEPROSITE-ProRule annotationAdd BLAST200

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi40 – 43Poly-Ser4

Sequence similaritiesi

Contains 1 MAGE domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000231161.
HOVERGENiHBG006315.
InParanoidiP43357.
OMAiIAREGDC.
OrthoDBiEOG091G0TJ0.
PhylomeDBiP43357.
TreeFamiTF328505.

Family and domain databases

InterProiIPR021072. MAGE_N.
IPR030097. MAGEA3/MAGEA6.
IPR002190. MHD_dom.
[Graphical view]
PANTHERiPTHR11736:SF60. PTHR11736:SF60. 1 hit.
PfamiPF01454. MAGE. 1 hit.
PF12440. MAGE_N. 1 hit.
[Graphical view]
SMARTiSM01373. MAGE. 1 hit.
SM01392. MAGE_N. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43357-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLEQRSQHC KPEEGLEARG EALGLVGAQA PATEEQEAAS SSSTLVEVTL
60 70 80 90 100
GEVPAAESPD PPQSPQGASS LPTTMNYPLW SQSYEDSSNQ EEEGPSTFPD
110 120 130 140 150
LESEFQAALS RKVAELVHFL LLKYRAREPV TKAEMLGSVV GNWQYFFPVI
160 170 180 190 200
FSKASSSLQL VFGIELMEVD PIGHLYIFAT CLGLSYDGLL GDNQIMPKAG
210 220 230 240 250
LLIIVLAIIA REGDCAPEEK IWEELSVLEV FEGREDSILG DPKKLLTQHF
260 270 280 290 300
VQENYLEYRQ VPGSDPACYE FLWGPRALVE TSYVKVLHHM VKISGGPHIS
310
YPPLHEWVLR EGEE
Length:314
Mass (Da):34,747
Last modified:November 1, 1995 - v1
Checksum:i3F5EB13D1C9946A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03735 Genomic DNA. Translation: AAA17446.1.
U82671 Genomic DNA. No translation available.
CR541767 mRNA. Translation: CAG46566.1.
CR541774 mRNA. Translation: CAG46573.1.
AK292384 mRNA. Translation: BAF85073.1.
BC000340 mRNA. Translation: AAH00340.1.
BC005963 mRNA. Translation: AAH05963.1.
BC011744 mRNA. Translation: AAH11744.1.
BC016803 mRNA. Translation: AAH16803.1.
BC017389 mRNA. Translation: AAH17389.1.
CCDSiCCDS76045.1.
PIRiJC2361.
RefSeqiNP_005353.1. NM_005362.3.
XP_005274733.1. XM_005274676.3.
XP_006724881.1. XM_006724818.2.
XP_011529462.1. XM_011531160.2.
XP_011529463.1. XM_011531161.2.
UniGeneiHs.417816.

Genome annotation databases

EnsembliENST00000370278; ENSP00000359301; ENSG00000221867.
ENST00000598245; ENSP00000473093; ENSG00000221867.
GeneIDi4102.
KEGGihsa:4102.
UCSCiuc033faf.1. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03735 Genomic DNA. Translation: AAA17446.1.
U82671 Genomic DNA. No translation available.
CR541767 mRNA. Translation: CAG46566.1.
CR541774 mRNA. Translation: CAG46573.1.
AK292384 mRNA. Translation: BAF85073.1.
BC000340 mRNA. Translation: AAH00340.1.
BC005963 mRNA. Translation: AAH05963.1.
BC011744 mRNA. Translation: AAH11744.1.
BC016803 mRNA. Translation: AAH16803.1.
BC017389 mRNA. Translation: AAH17389.1.
CCDSiCCDS76045.1.
PIRiJC2361.
RefSeqiNP_005353.1. NM_005362.3.
XP_005274733.1. XM_005274676.3.
XP_006724881.1. XM_006724818.2.
XP_011529462.1. XM_011531160.2.
XP_011529463.1. XM_011531161.2.
UniGeneiHs.417816.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QEWX-ray2.20C271-279[»]
4V0PX-ray2.07A104-314[»]
5BRZX-ray2.62C168-176[»]
ProteinModelPortaliP43357.
SMRiP43357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110276. 5 interactors.
DIPiDIP-44886N.
IntActiP43357. 14 interactors.
MINTiMINT-1537705.
STRINGi9606.ENSP00000359301.

PTM databases

iPTMnetiP43357.
PhosphoSitePlusiP43357.

Polymorphism and mutation databases

BioMutaiMAGEA3.
DMDMi1170857.

Proteomic databases

MaxQBiP43357.
PaxDbiP43357.
PeptideAtlasiP43357.
PRIDEiP43357.

Protocols and materials databases

DNASUi4102.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000370278; ENSP00000359301; ENSG00000221867.
ENST00000598245; ENSP00000473093; ENSG00000221867.
GeneIDi4102.
KEGGihsa:4102.
UCSCiuc033faf.1. human.

Organism-specific databases

CTDi4102.
DisGeNETi4102.
GeneCardsiMAGEA3.
HGNCiHGNC:6801. MAGEA3.
MIMi300174. gene.
neXtProtiNX_P43357.
OpenTargetsiENSG00000221867.
PharmGKBiPA30547.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000231161.
HOVERGENiHBG006315.
InParanoidiP43357.
OMAiIAREGDC.
OrthoDBiEOG091G0TJ0.
PhylomeDBiP43357.
TreeFamiTF328505.

Enzyme and pathway databases

BioCyciZFISH:G66-33124-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP43357.
GeneWikiiMAGEA3.
GenomeRNAii4102.
PROiP43357.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000221867.
CleanExiHS_MAGEA3.
ExpressionAtlasiP43357. baseline and differential.
GenevisibleiP43357. HS.

Family and domain databases

InterProiIPR021072. MAGE_N.
IPR030097. MAGEA3/MAGEA6.
IPR002190. MHD_dom.
[Graphical view]
PANTHERiPTHR11736:SF60. PTHR11736:SF60. 1 hit.
PfamiPF01454. MAGE. 1 hit.
PF12440. MAGE_N. 1 hit.
[Graphical view]
SMARTiSM01373. MAGE. 1 hit.
SM01392. MAGE_N. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAGA3_HUMAN
AccessioniPrimary (citable) accession number: P43357
Secondary accession number(s): Q6FHI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

In vitro experiments by PubMed:17942928 measuring cell viability in melanoma cell lines used siRNA specific for MAGEA3 and MAGEA6.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.