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Protein

Melanoma-associated antigen 2

Gene

MAGEA2

more
Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reduces p53/TP53 transactivation function through recruitment of HDAC3 to p53/TP53 transcription sites. Also represses p73/TP73 activity. Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination by TRIM28 potentially in presence of Ubl-conjugating enzyme UBE2H. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. Negatively regulates acetylation and sumoylation of PML and represses PML-induced p53/TP53 acetylation and activation.4 Publications

GO - Molecular functioni

  • histone deacetylase binding Source: UniProtKB
  • repressing transcription factor binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • cellular protein catabolic process Source: UniProtKB
  • cellular senescence Source: UniProtKB
  • negative regulation of protein acetylation Source: UniProtKB
  • negative regulation of protein sumoylation Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: UniProtKB
  • positive regulation of ubiquitin-protein transferase activity Source: UniProtKB
  • signal transduction by p53 class mediator Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionTumor antigen
Biological processTranscription, Transcription regulation, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma-associated antigen 2
Alternative name(s):
Cancer/testis antigen 1.2
Short name:
CT1.2
MAGE-2 antigen
Gene namesi
Name:MAGEA2
Synonyms:MAGE2, MAGEA2A
AND
Name:MAGEA2B
Synonyms:MAGE2, MAGEA2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000183305.13
HostDB:ENSG00000268606.5
HGNCiHGNC:6800 MAGEA2
HGNC:19340 MAGEA2B
MIMi300173 gene
300549 gene
neXtProtiNX_P43356

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi170V → D: Improves ability to bind to HLA-A1. 1 Publication1

Organism-specific databases

DisGeNETi266740
4101
OpenTargetsiENSG00000183305
PharmGKBiPA30546

Polymorphism and mutation databases

BioMutaiMAGEA2
DMDMi1170856

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001567021 – 314Melanoma-associated antigen 2Add BLAST314

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43356
PaxDbiP43356
PeptideAtlasiP43356
PRIDEiP43356

PTM databases

iPTMnetiP43356
PhosphoSitePlusiP43356

Expressioni

Tissue specificityi

Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes.

Gene expression databases

BgeeiENSG00000183305
CleanExiHS_MAGEA2
ExpressionAtlasiP43356 baseline and differential
GenevisibleiP43356 HS

Interactioni

Subunit structurei

Interacts with TRIM28 and UBE2H. Interacts with HDAC3. Interacts with PML (isoform PML-1, isoform PML-2, isoform PML-3, isoform PML-4 and isoform PML-5).3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • histone deacetylase binding Source: UniProtKB
  • repressing transcription factor binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi110275, 6 interactors
129334, 10 interactors
DIPiDIP-61231N
IntActiP43356, 25 interactors
STRINGi9606.ENSP00000359307

Structurei

3D structure databases

ProteinModelPortaliP43356
SMRiP43356
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 308MAGEPROSITE-ProRule annotationAdd BLAST200

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi40 – 43Poly-Ser4

Phylogenomic databases

eggNOGiKOG4562 Eukaryota
ENOG4111S70 LUCA
GeneTreeiENSGT00760000118824
HOGENOMiHOG000231161
HOVERGENiHBG006315
InParanoidiP43356
OMAiISGEPHI
OrthoDBiEOG091G0TJ0
PhylomeDBiP43356
TreeFamiTF328505

Family and domain databases

InterProiView protein in InterPro
IPR037445 MAGE
IPR021072 MAGE_N
IPR002190 MHD_dom
PANTHERiPTHR11736 PTHR11736, 1 hit
PfamiView protein in Pfam
PF01454 MAGE, 2 hits
PF12440 MAGE_N, 1 hit
SMARTiView protein in SMART
SM01373 MAGE, 1 hit
SM01392 MAGE_N, 1 hit
PROSITEiView protein in PROSITE
PS50838 MAGE, 1 hit

Sequencei

Sequence statusi: Complete.

P43356-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLEQRSQHC KPEEGLEARG EALGLVGAQA PATEEQQTAS SSSTLVEVTL
60 70 80 90 100
GEVPAADSPS PPHSPQGASS FSTTINYTLW RQSDEGSSNQ EEEGPRMFPD
110 120 130 140 150
LESEFQAAIS RKMVELVHFL LLKYRAREPV TKAEMLESVL RNCQDFFPVI
160 170 180 190 200
FSKASEYLQL VFGIEVVEVV PISHLYILVT CLGLSYDGLL GDNQVMPKTG
210 220 230 240 250
LLIIVLAIIA IEGDCAPEEK IWEELSMLEV FEGREDSVFA HPRKLLMQDL
260 270 280 290 300
VQENYLEYRQ VPGSDPACYE FLWGPRALIE TSYVKVLHHT LKIGGEPHIS
310
YPPLHERALR EGEE
Length:314
Mass (Da):35,055
Last modified:November 1, 1995 - v1
Checksum:i844F16335A2BECE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L18920 Genomic DNA Translation: AAA17729.1
AK290443 mRNA Translation: BAF83132.1
U82671 Genomic DNA No translation available.
CH471169 Genomic DNA Translation: EAW99429.1
BC108720 mRNA Translation: AAI08721.1
BC112158 mRNA Translation: AAI12159.1
BC112160 mRNA Translation: AAI12161.1
CCDSiCCDS76046.1
CCDS76049.1
PIRiI68889
RefSeqiNP_001269430.1, NM_001282501.1
NP_001269431.1, NM_001282502.1
NP_001269433.1, NM_001282504.1
NP_001269434.1, NM_001282505.1
NP_001308329.1, NM_001321400.1
NP_001308330.1, NM_001321401.1
NP_001308331.1, NM_001321402.1
NP_001308332.1, NM_001321403.1
NP_001308333.1, NM_001321404.1
NP_005352.1, NM_005361.3
NP_705692.1, NM_153488.4
NP_786884.1, NM_175742.2
NP_786885.1, NM_175743.2
XP_016884895.1, XM_017029406.1
XP_016885008.1, XM_017029519.1
UniGeneiHs.534597
Hs.713061
Hs.736992

Genome annotation databases

EnsembliENST00000331220; ENSP00000333487; ENSG00000183305
ENST00000370293; ENSP00000359316; ENSG00000183305
ENST00000595583; ENSP00000470872; ENSG00000268606
ENST00000598543; ENSP00000469919; ENSG00000268606
ENST00000611557; ENSP00000480738; ENSG00000268606
ENST00000611674; ENSP00000480491; ENSG00000268606
ENST00000620710; ENSP00000484290; ENSG00000268606
ENST00000623438; ENSP00000485391; ENSG00000268606
ENST00000623806; ENSP00000485541; ENSG00000268606
GeneIDi266740
4101
KEGGihsa:266740
hsa:4101
UCSCiuc004fgg.3 human

Similar proteinsi

Entry informationi

Entry nameiMAGA2_HUMAN
AccessioniPrimary (citable) accession number: P43356
Secondary accession number(s): A8K328, Q32NC6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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