P43355 (MAGA1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Melanoma-associated antigen 1

Alternative name(s):
Antigen MZ2-E
Cancer/testis antigen 1.1
Short name=CT1.1
MAGE-1 antigen

Gene names

Name:	MAGEA1
Synonyms:	MAGE1, MAGE1A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
Subcellular location	Cytoplasm Ref.11.
Tissue specificity	Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. Never expressed in kidney tumors, leukemias and lymphomas.
Sequence similarities	Contains 1 MAGE domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Molecular function	Tumor antigen
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Melanoma-associated antigen 1		PRO_0000156701
Regions
	Domain	102 – 301	200	MAGE
	Compositional bias	33 – 36	4	Poly-Ser
Natural variations
	Natural variant	32	1	T → A Probable polymorphism. Ref.3 Ref.4 Ref.9 Corresponds to variant rs2008160 [ dbSNP | Ensembl ].		VAR_004283
	Natural variant	63	1	A → T. Corresponds to variant rs2233044 [ dbSNP | Ensembl ].		VAR_053491
	Natural variant	72	1	R → Q. Corresponds to variant rs2008144 [ dbSNP | Ensembl ].		VAR_011737
	Natural variant	278	1	K → T in a breast cancer sample; somatic mutation. Ref.15		VAR_036581
Experimental info
	Mutagenesis	163	1	D → A: Abolishes HLA-A1 binding.
	Mutagenesis	169	1	Y → A: Abolishes HLA-A1 binding.

Sequences

	Sequence		Length	Mass (Da)
	P43355 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 544EEB1F9F4E9D33	FASTA	309	34,342
	10 20 30 40 50 60 MSLEQRSLHC KPEEALEAQQ EALGLVCVQA ATSSSSPLVL GTLEEVPTAG STDPPQSPQG 70 80 90 100 110 120 ASAFPTTINF TRQRQPSEGS SSREEEGPST SCILESLFRA VITKKVADLV GFLLLKYRAR 130 140 150 160 170 180 EPVTKAEMLE SVIKNYKHCF PEIFGKASES LQLVFGIDVK EADPTGHSYV LVTCLGLSYD 190 200 210 220 230 240 GLLGDNQIMP KTGFLIIVLV MIAMEGGHAP EEEIWEELSV MEVYDGREHS AYGEPRKLLT 250 260 270 280 290 300 QDLVQEKYLE YRQVPDSDPA RYEFLWGPRA LAETSYVKVL EYVIKVSARV RFFFPSLREA ALREEEEGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma." van der Bruggen P., Traversari C., Chomez P., Lurquin C., De Plaen E., van den Eynde B., Knuth A., Boon T. Science 254:1643-1647(1991) [PubMed: 1840703] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and analysis of MAGE-1-related genes." Ding M., Beck R.J., Keller C.J., Fenton R.G. Biochem. Biophys. Res. Commun. 202:549-555(1994) [PubMed: 8037761] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skin.
[3]	"The genetic polymorphism of melanoma-associated antigen 1 in Chinese normal donors and hepatoma patients." Wang L.P., Chen H.S., Mei M.H., Qin L.L., Cong X., Fei R., Xu J., Wei L. Zhonghua Gan Zang Bing Za Zhi 12:151-155(2004) [PubMed: 15059299] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-32.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-32.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[6]	"Comparative genome sequence analysis of the Bpa/Str region in mouse and man." Mallon A.-M., Platzer M., Bate R., Gloeckner G., Botcherby M.R.M., Nordsiek G., Strivens M.A., Kioschis P., Dangel A., Cunningham D., Straw R.N.A., Weston P., Gilbert M., Fernando S., Goodall K., Hunter G., Greystrong J.S., Clarke D. , Kimberley C., Goerdes M., Blechschmidt K., Rump A., Hinzmann B., Mundy C.R., Miller W., Poustka A., Herman G.E., Rhodes M., Denny P., Rosenthal A., Brown S.D.M. Genome Res. 10:758-775(2000) [PubMed: 10854409] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed: 15772651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-32. Tissue: Skin.
[10]	"Human gene MAGE-3 codes for an antigen recognized on a melanoma by autologous cytolytic T lymphocytes." Gaugler B., van den Eynde B., van der Bruggen P., Romero P., Gaforio J.J., De Plaen E., Lethe B.G., Brasseur F., Boon T. J. Exp. Med. 179:921-930(1994) [PubMed: 8113684] [Abstract] Cited for: MUTAGENESIS. Tissue: Blood.
[11]	"MAGE-1 gene product is a cytoplasmic protein." Schultz-Thater E., Juretic A., Dellabona P., Luscher U., Siegrist W., Harder F., Heberer M., Zuber M., Spagnoli G.C. Int. J. Cancer 59:435-439(1994) [PubMed: 7927954] [Abstract] Cited for: SUBCELLULAR LOCATION.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"A major histocompatibility complex-peptide-restricted antibody and t cell receptor molecules recognize their target by distinct binding modes: crystal structure of human leukocyte antigen (HLA)-A1-MAGE-A1 in complex with FAB-HYB3." Hulsmeyer M., Chames P., Hillig R.C., Stanfield R.L., Held G., Coulie P.G., Alings C., Wille G., Saenger W., Uchanska-Ziegler B., Hoogenboom H.R., Ziegler A. J. Biol. Chem. 280:2972-2980(2005) [PubMed: 15537658] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 161-169 IN COMPLEX WITH HLA CLASS I HISTOCOMPATIBILITY COMPLEX.
[14]	"Conformational changes within the HLA-A1:MAGE-A1 complex induced by binding of a recombinant antibody fragment with TCR-like specificity." Kumar P., Vahedi-Faridi A., Saenger W., Ziegler A., Uchanska-Ziegler B. Protein Sci. 18:37-49(2009) [PubMed: 19177349] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 161-169 IN COMPLEX WITH HLA CLASS I HISTOCOMPATIBILITY COMPLEX.
[15]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-278.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M77481 Genomic DNA. Translation: AAA03229.1.
AY148486 mRNA. Translation: AAN62752.1.
BT009789 mRNA. Translation: AAP88791.1.
AK314979 mRNA. Translation: BAG37478.1.
U82670 Genomic DNA. No translation available.
CH471172 Genomic DNA. Translation: EAW72885.1.
BC017555 mRNA. Translation: AAH17555.1.

IPI

IPI00301082.

PIR

JC2358.

RefSeq

NP_004979.3. NM_004988.4.

UniGene

Hs.72879.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1W72	X-ray	2.15	C/F	161-169	[»]
3BO8	X-ray	1.80	C	161-169	[»]

ProteinModelPortal

P43355.

SMR

P43355. Positions 95-304.

ModBase

Search...

Protein-protein interaction databases

IntAct

P43355. 5 interactions.

MINT

MINT-1455313.

STRING

P43355.

PTM databases

PhosphoSite

P43355.

Polymorphism databases

DMDM

1170855.

Proteomic databases

PRIDE

P43355.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000356661; ENSP00000349085; ENSG00000198681.

GeneID

4100.

KEGG

hsa:4100.

UCSC

uc004fhf.2. human.

Organism-specific databases

CTD

4100.

GeneCards

GC0XM152481.

H-InvDB

HIX0017126.

HGNC

HGNC:6796. MAGEA1.

HPA

CAB002620.

MIM

300016. gene.

neXtProt

NX_P43355.

PharmGKB

PA30542.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG10888.

HOGENOM

HBG507249.

HOVERGEN

HBG006315.

InParanoid

P43355.

OMA

FPTTINF.

OrthoDB

EOG47H5QM.

PhylomeDB

P43355.

Gene expression databases

ArrayExpress

P43355.

Bgee

P43355.

CleanEx

HS_MAGEA1.

Genevestigator

P43355.

GermOnline

ENSG00000198681. Homo sapiens.

Family and domain databases

InterPro

IPR002190. MAGE.
IPR021072. Melanoma_ass_antigen_N.
[Graphical view]

PANTHER

PTHR11736. MAGE. 1 hit.

Pfam

PF01454. MAGE. 1 hit.
PF12440. MAGE_N. 1 hit.
[Graphical view]

PROSITE

PS50838. MAGE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

16080.

SOURCE

Search...

Entry information

Entry name

MAGA1_HUMAN

Accession

Primary (citable) accession number: P43355
Secondary accession number(s): B2RC81, O00346

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.