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Protein

DNA repair protein RAD52 homolog

Gene

Rad52

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi153 – 1575By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_298118. Assembly of the RAD51-ssDNA nucleoprotein complex.
REACT_301691. Presynaptic phase of homologous DNA pairing and strand exchange.
REACT_360954. SUMOylation of DNA damage response and repair proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD52 homolog
Gene namesi
Name:Rad52
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:101949. Rad52.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 420420DNA repair protein RAD52 homologPRO_0000173882Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei105 – 1051Phosphotyrosine; by ABL1By similarity
Modified residuei200 – 2001PhosphoserineBy similarity
Modified residuei339 – 3391PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylated upon DNA damage by ABL1, and probably by ATM or ATR.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP43352.
PRIDEiP43352.

PTM databases

PhosphoSiteiP43352.

Expressioni

Gene expression databases

BgeeiP43352.
CleanExiMM_RAD52.
ExpressionAtlasiP43352. baseline and differential.
GenevisibleiP43352. MM.

Interactioni

Subunit structurei

The full-length protein forms heptameric rings. Interacts with ABL1. Interacts with RPA2; the interaction is direct and associates RAD52 with the RPA complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000125502.

Structurei

3D structure databases

ProteinModelPortaliP43352.
SMRiP43352. Positions 25-210.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni222 – 29170Mediates interaction with RPA2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the RAD52 family.Curated

Phylogenomic databases

eggNOGiCOG5055.
GeneTreeiENSGT00390000008766.
HOVERGENiHBG054676.
InParanoidiP43352.
KOiK10873.
OMAiQARYSSC.
OrthoDBiEOG74XS87.
PhylomeDBiP43352.
TreeFamiTF101221.

Family and domain databases

InterProiIPR004585. DNA_recomb/repair_Rad52.
IPR007232. Rad52_Rad59_Rad22.
[Graphical view]
PANTHERiPTHR12132. PTHR12132. 1 hit.
PTHR12132:SF1. PTHR12132:SF1. 1 hit.
PfamiPF04098. Rad52_Rad22. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00607. rad52. 1 hit.

Sequencei

Sequence statusi: Complete.

P43352-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGPEEAVHR GCDNHPPFVG GKSVLLFGQS QYTADEYQAI QKALRQRLGP
60 70 80 90 100
EYISSRMAGG GQKVCYIEGH RVINLANEMF GYNGWAHSIT QQNVDFVDLN
110 120 130 140 150
NGKFYVGVCA FVKVQLKDGS YHEDVGYGVS EGLRSKALSL EKARKEAVTD
160 170 180 190 200
GLKRALRSFG NALGNCILDK DYLRSLNKLP RQLPLDVDLT KTKREDFEPS
210 220 230 240 250
VEQARYNSCR QNEALGLPKP QEVTSPCRSS PPHDSNIKLQ GAKDISSSCS
260 270 280 290 300
LAATLESDAT HQRKLRKLRQ KQLQQQFREQ METRRQSHAP AEEVAAKHAA
310 320 330 340 350
VLPAPPKHST PVTAASELLQ EKVVFPDNLE ENLEMWDLTP DLEDIIKPLC
360 370 380 390 400
RAEPAQTSAT RTFNNQDSVP HIHCHQKPQE KPGPGHLQTC NTNQHVLGSR
410 420
EDSEPHRKSQ DLKKRKLDPS
Length:420
Mass (Da):46,910
Last modified:November 1, 1995 - v1
Checksum:i84514C8579072F98
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti195 – 1951E → K in AAA85794 (PubMed:7774919).Curated
Sequence conflicti300 – 3001Missing in AAA85794 (PubMed:7774919).Curated
Sequence conflicti352 – 3521A → G (PubMed:7829098).Curated
Sequence conflicti352 – 3521A → G (Ref. 4) Curated
Sequence conflicti401 – 4011Missing (PubMed:7829098).Curated
Sequence conflicti401 – 4011Missing (Ref. 4) Curated
Sequence conflicti418 – 4181D → N in CAA83659 (PubMed:7526206).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06837 mRNA. Translation: AAA75441.1.
U12135 mRNA. Translation: AAA85794.1.
Z32767 mRNA. Translation: CAA83659.1.
AF004854 Genomic DNA. Translation: AAB69174.1.
CCDSiCCDS51887.1.
PIRiA55727.
A56529.
RefSeqiNP_001159853.1. NM_001166381.1.
UniGeneiMm.149.

Genome annotation databases

EnsembliENSMUST00000162461; ENSMUSP00000125502; ENSMUSG00000030166.
GeneIDi19365.
KEGGimmu:19365.
UCSCiuc009dmq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06837 mRNA. Translation: AAA75441.1.
U12135 mRNA. Translation: AAA85794.1.
Z32767 mRNA. Translation: CAA83659.1.
AF004854 Genomic DNA. Translation: AAB69174.1.
CCDSiCCDS51887.1.
PIRiA55727.
A56529.
RefSeqiNP_001159853.1. NM_001166381.1.
UniGeneiMm.149.

3D structure databases

ProteinModelPortaliP43352.
SMRiP43352. Positions 25-210.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000125502.

PTM databases

PhosphoSiteiP43352.

Proteomic databases

PaxDbiP43352.
PRIDEiP43352.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000162461; ENSMUSP00000125502; ENSMUSG00000030166.
GeneIDi19365.
KEGGimmu:19365.
UCSCiuc009dmq.2. mouse.

Organism-specific databases

CTDi5893.
MGIiMGI:101949. Rad52.

Phylogenomic databases

eggNOGiCOG5055.
GeneTreeiENSGT00390000008766.
HOVERGENiHBG054676.
InParanoidiP43352.
KOiK10873.
OMAiQARYSSC.
OrthoDBiEOG74XS87.
PhylomeDBiP43352.
TreeFamiTF101221.

Enzyme and pathway databases

ReactomeiREACT_298118. Assembly of the RAD51-ssDNA nucleoprotein complex.
REACT_301691. Presynaptic phase of homologous DNA pairing and strand exchange.
REACT_360954. SUMOylation of DNA damage response and repair proteins.

Miscellaneous databases

NextBioi296437.
PROiP43352.
SOURCEiSearch...

Gene expression databases

BgeeiP43352.
CleanExiMM_RAD52.
ExpressionAtlasiP43352. baseline and differential.
GenevisibleiP43352. MM.

Family and domain databases

InterProiIPR004585. DNA_recomb/repair_Rad52.
IPR007232. Rad52_Rad59_Rad22.
[Graphical view]
PANTHERiPTHR12132. PTHR12132. 1 hit.
PTHR12132:SF1. PTHR12132:SF1. 1 hit.
PfamiPF04098. Rad52_Rad22. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00607. rad52. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Identification of a mouse homologue of the Saccharomyces cerevisiae recombination and repair gene, RAD52."
    Bendixen C., Sunjevaric I., Bauchwitz R., Rothstein R.
    Genomics 23:300-303(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "The human and mouse homologs of the yeast RAD52 gene: cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues."
    Shen Z., Denison K., Lobb R., Gatewood J.M., Chen D.J.
    Genomics 25:199-206(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Thymus.
  4. Sunjevaric I., Bendixen C., Mortensen U.H., Miljkovic V., Rothstein R.
    Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6J.

Entry informationi

Entry nameiRAD52_MOUSE
AccessioniPrimary (citable) accession number: P43352
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 24, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.