P43352 (RAD52_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA repair protein RAD52 homolog

Gene names

Name:

Rad52

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase By similarity.
Subunit structure	Forms a undecameric ring By similarity. Interacts with ABL1 By similarity.
Subcellular location	Nucleus Potential.
Post-translational modification	Phosphorylated upon DNA damage by ABL1, and probably by ATM or ATR By similarity.
Sequence similarities	Belongs to the RAD52 family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	double-strand break repair via homologous recombination Inferred from direct assay PubMed 12379650. Source: MGI
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	DNA repair protein RAD52 homolog		PRO_0000173882
Amino acid modifications
	Modified residue	105	1	Phosphotyrosine; by ABL1 By similarity
	Modified residue	200	1	Phosphoserine By similarity
	Modified residue	339	1	Phosphothreonine By similarity
Experimental info
	Sequence conflict	195	1	E → K in AAA85794. Ref.2
	Sequence conflict	300	1	Missing in AAA85794. Ref.2
	Sequence conflict	352	1	A → G Ref.1
	Sequence conflict	352	1	A → G Ref.4
	Sequence conflict	401	1	Missing Ref.1
	Sequence conflict	401	1	Missing Ref.4
	Sequence conflict	418	1	D → N in CAA83659. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P43352 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 84514C8579072F98	FASTA	420	46,910
	10 20 30 40 50 60 MAGPEEAVHR GCDNHPPFVG GKSVLLFGQS QYTADEYQAI QKALRQRLGP EYISSRMAGG 70 80 90 100 110 120 GQKVCYIEGH RVINLANEMF GYNGWAHSIT QQNVDFVDLN NGKFYVGVCA FVKVQLKDGS 130 140 150 160 170 180 YHEDVGYGVS EGLRSKALSL EKARKEAVTD GLKRALRSFG NALGNCILDK DYLRSLNKLP 190 200 210 220 230 240 RQLPLDVDLT KTKREDFEPS VEQARYNSCR QNEALGLPKP QEVTSPCRSS PPHDSNIKLQ 250 260 270 280 290 300 GAKDISSSCS LAATLESDAT HQRKLRKLRQ KQLQQQFREQ METRRQSHAP AEEVAAKHAA 310 320 330 340 350 360 VLPAPPKHST PVTAASELLQ EKVVFPDNLE ENLEMWDLTP DLEDIIKPLC RAEPAQTSAT 370 380 390 400 410 420 RTFNNQDSVP HIHCHQKPQE KPGPGHLQTC NTNQHVLGSR EDSEPHRKSQ DLKKRKLDPS

References

[1]	"Identification of a mouse homologue of the Saccharomyces cerevisiae recombination and repair gene, RAD52." Bendixen C., Sunjevaric I., Bauchwitz R., Rothstein R. Genomics 23:300-303(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
[2]	"The human and mouse homologs of the yeast RAD52 gene: cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues." Shen Z., Denison K., Lobb R., Gatewood J.M., Chen D.J. Genomics 25:199-206(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C3H.
[3]	"Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination." Muris D.F., Bezzubova O., Buerstedde J.-M., Vreeken K., Balajee A.S., Osgood C.J., Troelstra C., Hoeijmakers J.H., Ostermann K., Schmidt H., Natarajan A.T., Eeken J.C.J., Lohman P.H.M., Pastink A. Mutat. Res. 315:295-305(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA. Tissue: Thymus.
[4]	Sunjevaric I., Bendixen C., Mortensen U.H., Miljkovic V., Rothstein R. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: C57BL/6J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U06837 mRNA. Translation: AAA75441.1. U12135 mRNA. Translation: AAA85794.1. Z32767 mRNA. Translation: CAA83659.1. AF004854 Genomic DNA. Translation: AAB69174.1.
IPI	IPI00119277.
PIR	A55727. A56529.
RefSeq	NP_001159853.1. NM_001166381.1.
UniGene	Mm.149.
3D structure databases
ProteinModelPortal	P43352.
SMR	P43352. Positions 25-210.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000032269.
PTM databases
PhosphoSite	P43352.
Proteomic databases
PaxDb	P43352.
PRIDE	P43352.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000162461; ENSMUSP00000125502; ENSMUSG00000030166.
GeneID	19365.
KEGG	mmu:19365.
UCSC	uc009dmq.2. mouse.
Organism-specific databases
CTD	5893.
MGI	MGI:101949. Rad52.
Phylogenomic databases
eggNOG	COG5055.
GeneTree	ENSGT00390000008766.
HOVERGEN	HBG054676.
InParanoid	P43352.
KO	K10873.
OMA	RKSQDMK.
OrthoDB	EOG451DR1.
Gene expression databases
ArrayExpress	P43352.
Bgee	P43352.
CleanEx	MM_RAD52.
Genevestigator	P43352.
GermOnline	ENSMUSG00000030166. Mus musculus.
Family and domain databases
InterPro	IPR004585. DNA_recomb/repair_Rad52. IPR007232. Rad52_Rad22. [Graphical view]
PANTHER	PTHR12132. PTHR12132. 1 hit.
Pfam	PF04098. Rad52_Rad22. 1 hit. [Graphical view]
TIGRFAMs	TIGR00607. rad52. 1 hit.
ProtoNet	Search...
Other
NextBio	296437.
SOURCE	Search...

Entry information

Entry name

RAD52_MOUSE

Accession

Primary (citable) accession number: P43352

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program