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Protein

DNA repair protein RAD52 homolog

Gene

Rad52

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi153 – 157By similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-5685938. HDR through Single Strand Annealing (SSA).

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD52 homolog
Gene namesi
Name:Rad52
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:101949. Rad52.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738821 – 420DNA repair protein RAD52 homologAdd BLAST420

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei105Phosphotyrosine; by ABL1By similarity1
Modified residuei200PhosphoserineBy similarity1
Modified residuei339PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated upon DNA damage by ABL1, and probably by ATM or ATR.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP43352.
PRIDEiP43352.

PTM databases

iPTMnetiP43352.
PhosphoSitePlusiP43352.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030166.
CleanExiMM_RAD52.
ExpressionAtlasiP43352. baseline and differential.
GenevisibleiP43352. MM.

Interactioni

Subunit structurei

The full-length protein forms heptameric rings. Interacts with ABL1. Interacts with RPA2; the interaction is direct and associates RAD52 with the RPA complex (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000125502.

Structurei

3D structure databases

ProteinModelPortaliP43352.
SMRiP43352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni222 – 291Mediates interaction with RPA2By similarityAdd BLAST70

Sequence similaritiesi

Belongs to the RAD52 family.Curated

Phylogenomic databases

eggNOGiKOG4141. Eukaryota.
COG5055. LUCA.
GeneTreeiENSGT00390000008766.
HOVERGENiHBG054676.
InParanoidiP43352.
KOiK10873.
OMAiQARYSSC.
OrthoDBiEOG091G0OIP.
PhylomeDBiP43352.
TreeFamiTF101221.

Family and domain databases

InterProiIPR004585. DNA_recomb/repair_Rad52.
IPR007232. Rad52_Rad59_Rad22.
[Graphical view]
PANTHERiPTHR12132. PTHR12132. 1 hit.
PfamiPF04098. Rad52_Rad22. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00607. rad52. 1 hit.

Sequencei

Sequence statusi: Complete.

P43352-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGPEEAVHR GCDNHPPFVG GKSVLLFGQS QYTADEYQAI QKALRQRLGP
60 70 80 90 100
EYISSRMAGG GQKVCYIEGH RVINLANEMF GYNGWAHSIT QQNVDFVDLN
110 120 130 140 150
NGKFYVGVCA FVKVQLKDGS YHEDVGYGVS EGLRSKALSL EKARKEAVTD
160 170 180 190 200
GLKRALRSFG NALGNCILDK DYLRSLNKLP RQLPLDVDLT KTKREDFEPS
210 220 230 240 250
VEQARYNSCR QNEALGLPKP QEVTSPCRSS PPHDSNIKLQ GAKDISSSCS
260 270 280 290 300
LAATLESDAT HQRKLRKLRQ KQLQQQFREQ METRRQSHAP AEEVAAKHAA
310 320 330 340 350
VLPAPPKHST PVTAASELLQ EKVVFPDNLE ENLEMWDLTP DLEDIIKPLC
360 370 380 390 400
RAEPAQTSAT RTFNNQDSVP HIHCHQKPQE KPGPGHLQTC NTNQHVLGSR
410 420
EDSEPHRKSQ DLKKRKLDPS
Length:420
Mass (Da):46,910
Last modified:November 1, 1995 - v1
Checksum:i84514C8579072F98
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti195E → K in AAA85794 (PubMed:7774919).Curated1
Sequence conflicti300Missing in AAA85794 (PubMed:7774919).Curated1
Sequence conflicti352A → G (PubMed:7829098).Curated1
Sequence conflicti352A → G (Ref. 4) Curated1
Sequence conflicti401Missing (PubMed:7829098).Curated1
Sequence conflicti401Missing (Ref. 4) Curated1
Sequence conflicti418D → N in CAA83659 (PubMed:7526206).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06837 mRNA. Translation: AAA75441.1.
U12135 mRNA. Translation: AAA85794.1.
Z32767 mRNA. Translation: CAA83659.1.
AF004854 Genomic DNA. Translation: AAB69174.1.
CCDSiCCDS51887.1.
PIRiA55727.
A56529.
RefSeqiNP_001159853.1. NM_001166381.1.
UniGeneiMm.149.

Genome annotation databases

EnsembliENSMUST00000162461; ENSMUSP00000125502; ENSMUSG00000030166.
GeneIDi19365.
KEGGimmu:19365.
UCSCiuc009dmq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06837 mRNA. Translation: AAA75441.1.
U12135 mRNA. Translation: AAA85794.1.
Z32767 mRNA. Translation: CAA83659.1.
AF004854 Genomic DNA. Translation: AAB69174.1.
CCDSiCCDS51887.1.
PIRiA55727.
A56529.
RefSeqiNP_001159853.1. NM_001166381.1.
UniGeneiMm.149.

3D structure databases

ProteinModelPortaliP43352.
SMRiP43352.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000125502.

PTM databases

iPTMnetiP43352.
PhosphoSitePlusiP43352.

Proteomic databases

PaxDbiP43352.
PRIDEiP43352.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000162461; ENSMUSP00000125502; ENSMUSG00000030166.
GeneIDi19365.
KEGGimmu:19365.
UCSCiuc009dmq.2. mouse.

Organism-specific databases

CTDi5893.
MGIiMGI:101949. Rad52.

Phylogenomic databases

eggNOGiKOG4141. Eukaryota.
COG5055. LUCA.
GeneTreeiENSGT00390000008766.
HOVERGENiHBG054676.
InParanoidiP43352.
KOiK10873.
OMAiQARYSSC.
OrthoDBiEOG091G0OIP.
PhylomeDBiP43352.
TreeFamiTF101221.

Enzyme and pathway databases

ReactomeiR-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-5685938. HDR through Single Strand Annealing (SSA).

Miscellaneous databases

PROiP43352.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030166.
CleanExiMM_RAD52.
ExpressionAtlasiP43352. baseline and differential.
GenevisibleiP43352. MM.

Family and domain databases

InterProiIPR004585. DNA_recomb/repair_Rad52.
IPR007232. Rad52_Rad59_Rad22.
[Graphical view]
PANTHERiPTHR12132. PTHR12132. 1 hit.
PfamiPF04098. Rad52_Rad22. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00607. rad52. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRAD52_MOUSE
AccessioniPrimary (citable) accession number: P43352
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.