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P43352 (RAD52_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA repair protein RAD52 homolog
Gene names
Name:Rad52
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase By similarity.

Subunit structure

The full-length protein forms heptameric rings. Interacts with ABL1. Interacts with RPA2; the interaction is direct and associates RAD52 with the RPA complex By similarity.

Subcellular location

Nucleus Potential.

Post-translational modification

Phosphorylated upon DNA damage by ABL1, and probably by ATM or ATR By similarity.

Sequence similarities

Belongs to the RAD52 family.

Ontologies

Keywords
   Biological processDNA damage
DNA recombination
DNA repair
   Cellular componentNucleus
   LigandDNA-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA recombination

Inferred from sequence or structural similarity. Source: UniProtKB

double-strand break repair via homologous recombination

Inferred from direct assay PubMed 12379650. Source: MGI

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 420420DNA repair protein RAD52 homolog
PRO_0000173882

Regions

DNA binding153 – 1575 By similarity
Region222 – 29170Mediates interaction with RPA2 By similarity

Amino acid modifications

Modified residue1051Phosphotyrosine; by ABL1 By similarity
Modified residue2001Phosphoserine By similarity
Modified residue3391Phosphothreonine By similarity

Experimental info

Sequence conflict1951E → K in AAA85794. Ref.2
Sequence conflict3001Missing in AAA85794. Ref.2
Sequence conflict3521A → G Ref.1
Sequence conflict3521A → G Ref.4
Sequence conflict4011Missing Ref.1
Sequence conflict4011Missing Ref.4
Sequence conflict4181D → N in CAA83659. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P43352 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 84514C8579072F98

FASTA42046,910
        10         20         30         40         50         60 
MAGPEEAVHR GCDNHPPFVG GKSVLLFGQS QYTADEYQAI QKALRQRLGP EYISSRMAGG 

        70         80         90        100        110        120 
GQKVCYIEGH RVINLANEMF GYNGWAHSIT QQNVDFVDLN NGKFYVGVCA FVKVQLKDGS 

       130        140        150        160        170        180 
YHEDVGYGVS EGLRSKALSL EKARKEAVTD GLKRALRSFG NALGNCILDK DYLRSLNKLP 

       190        200        210        220        230        240 
RQLPLDVDLT KTKREDFEPS VEQARYNSCR QNEALGLPKP QEVTSPCRSS PPHDSNIKLQ 

       250        260        270        280        290        300 
GAKDISSSCS LAATLESDAT HQRKLRKLRQ KQLQQQFREQ METRRQSHAP AEEVAAKHAA 

       310        320        330        340        350        360 
VLPAPPKHST PVTAASELLQ EKVVFPDNLE ENLEMWDLTP DLEDIIKPLC RAEPAQTSAT 

       370        380        390        400        410        420 
RTFNNQDSVP HIHCHQKPQE KPGPGHLQTC NTNQHVLGSR EDSEPHRKSQ DLKKRKLDPS 

« Hide

References

[1]"Identification of a mouse homologue of the Saccharomyces cerevisiae recombination and repair gene, RAD52."
Bendixen C., Sunjevaric I., Bauchwitz R., Rothstein R.
Genomics 23:300-303(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[2]"The human and mouse homologs of the yeast RAD52 gene: cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues."
Shen Z., Denison K., Lobb R., Gatewood J.M., Chen D.J.
Genomics 25:199-206(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C3H.
[3]"Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination."
Muris D.F., Bezzubova O., Buerstedde J.-M., Vreeken K., Balajee A.S., Osgood C.J., Troelstra C., Hoeijmakers J.H., Ostermann K., Schmidt H., Natarajan A.T., Eeken J.C.J., Lohman P.H.M., Pastink A.
Mutat. Res. 315:295-305(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6 X CBA.
Tissue: Thymus.
[4]Sunjevaric I., Bendixen C., Mortensen U.H., Miljkovic V., Rothstein R.
Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U06837 mRNA. Translation: AAA75441.1.
U12135 mRNA. Translation: AAA85794.1.
Z32767 mRNA. Translation: CAA83659.1.
AF004854 Genomic DNA. Translation: AAB69174.1.
CCDSCCDS51887.1.
PIRA55727.
A56529.
RefSeqNP_001159853.1. NM_001166381.1.
UniGeneMm.149.

3D structure databases

ProteinModelPortalP43352.
SMRP43352. Positions 25-210.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000032269.

PTM databases

PhosphoSiteP43352.

Proteomic databases

PaxDbP43352.
PRIDEP43352.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000162461; ENSMUSP00000125502; ENSMUSG00000030166.
GeneID19365.
KEGGmmu:19365.
UCSCuc009dmq.2. mouse.

Organism-specific databases

CTD5893.
MGIMGI:101949. Rad52.

Phylogenomic databases

eggNOGCOG5055.
GeneTreeENSGT00390000008766.
HOVERGENHBG054676.
InParanoidP43352.
KOK10873.
OMAQARYSSC.
OrthoDBEOG74XS87.
PhylomeDBP43352.
TreeFamTF101221.

Gene expression databases

BgeeP43352.
CleanExMM_RAD52.
GenevestigatorP43352.

Family and domain databases

InterProIPR004585. DNA_recomb/repair_Rad52.
IPR007232. Rad52_Rad22.
[Graphical view]
PANTHERPTHR12132. PTHR12132. 1 hit.
PfamPF04098. Rad52_Rad22. 1 hit.
[Graphical view]
TIGRFAMsTIGR00607. rad52. 1 hit.
ProtoNetSearch...

Other

NextBio296437.
PROP43352.
SOURCESearch...

Entry information

Entry nameRAD52_MOUSE
AccessionPrimary (citable) accession number: P43352
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot