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Protein

DNA repair protein RAD52 homolog

Gene

RAD52

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi152 – 1565

GO - Molecular functioni

  • DNA binding Source: MGI
  • identical protein binding Source: IntAct

GO - Biological processi

  • cellular response to DNA damage stimulus Source: MGI
  • cellular response to oxidative stress Source: MGI
  • DNA double-strand break processing involved in repair via single-strand annealing Source: MGI
  • DNA recombinase assembly Source: InterPro
  • DNA recombination Source: UniProtKB
  • double-strand break repair Source: ProtInc
  • protein sumoylation Source: Reactome
  • regulation of nucleotide-excision repair Source: MGI
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000002016-MONOMER.
ReactomeiR-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-5685938. HDR through Single Strand Annealing (SSA).
SIGNORiP43351.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD52 homolog
Gene namesi
Name:RAD52
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:9824. RAD52.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: Reactome
  • nucleus Source: MGI
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi55R → A: Abolishes ssDNA-binding. 1 Publication1
Mutagenesisi65Y → A: Moderately defective in both ss and dsDNA-binding. 1 Publication1
Mutagenesisi152K → A: Abolishes ssDNA-binding. 1 Publication1
Mutagenesisi153R → A: Moderately defective in both ss and dsDNA-binding. 1 Publication1
Mutagenesisi156R → A: Moderately defective in both ss and dsDNA-binding. 1 Publication1

Organism-specific databases

DisGeNETi5893.
OpenTargetsiENSG00000002016.
PharmGKBiPA34180.

Chemistry databases

ChEMBLiCHEMBL2362978.

Polymorphism and mutation databases

BioMutaiRAD52.
DMDMi1172823.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738811 – 418DNA repair protein RAD52 homologAdd BLAST418

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei104Phosphotyrosine; by ABL11 Publication1
Modified residuei199PhosphoserineCombined sources1
Modified residuei318PhosphothreonineCombined sources1
Modified residuei335PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated upon DNA damage by ABL1, and probably by ATM or ATR.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP43351.
PaxDbiP43351.
PeptideAtlasiP43351.
PRIDEiP43351.

PTM databases

iPTMnetiP43351.
PhosphoSitePlusiP43351.

Expressioni

Gene expression databases

BgeeiENSG00000002016.
CleanExiHS_RAD52.
ExpressionAtlasiP43351. baseline and differential.
GenevisibleiP43351. HS.

Organism-specific databases

HPAiCAB072847.
CAB072848.
HPA046628.

Interactioni

Subunit structurei

The full-length protein forms heptameric rings. Interacts with ABL1. Interacts with RPA2; the interaction is direct and associates RAD52 with the RPA complex.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-706448,EBI-706448
RAD51Q066093EBI-706448,EBI-297202
RPA3P352443EBI-706448,EBI-621428
WRNQ141919EBI-706448,EBI-368417

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi111830. 40 interactors.
DIPiDIP-333N.
IntActiP43351. 36 interactors.
MINTiMINT-2803258.
STRINGi9606.ENSP00000351284.

Structurei

Secondary structure

1418
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni26 – 28Combined sources3
Helixi33 – 43Combined sources11
Helixi49 – 51Combined sources3
Beta strandi52 – 57Combined sources6
Beta strandi60 – 66Combined sources7
Helixi68 – 79Combined sources12
Turni81 – 83Combined sources3
Beta strandi84 – 99Combined sources16
Beta strandi102 – 115Combined sources14
Beta strandi120 – 131Combined sources12
Helixi135 – 155Combined sources21
Helixi156 – 158Combined sources3
Helixi160 – 162Combined sources3
Helixi164 – 167Combined sources4
Helixi169 – 176Combined sources8
Helixi188 – 190Combined sources3
Helixi198 – 205Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H2IX-ray2.70A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V1-209[»]
1KN0X-ray2.85A/B/C/D/E/F/G/H/I/J/K1-212[»]
5JRBX-ray2.40A/B/C/D/E/F/G/H/I/J/K1-212[»]
DisProtiDP00437.
ProteinModelPortaliP43351.
SMRiP43351.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43351.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni221 – 280Mediates interaction with RPA21 PublicationAdd BLAST60

Sequence similaritiesi

Belongs to the RAD52 family.Curated

Phylogenomic databases

eggNOGiKOG4141. Eukaryota.
COG5055. LUCA.
GeneTreeiENSGT00390000008766.
HOGENOMiHOG000237354.
HOVERGENiHBG054676.
InParanoidiP43351.
KOiK10873.
OMAiQARYSSC.
OrthoDBiEOG091G0OIP.
PhylomeDBiP43351.
TreeFamiTF101221.

Family and domain databases

InterProiIPR004585. DNA_recomb/repair_Rad52.
IPR007232. Rad52_Rad59_Rad22.
[Graphical view]
PANTHERiPTHR12132. PTHR12132. 1 hit.
PfamiPF04098. Rad52_Rad22. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00607. rad52. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform alpha (identifier: P43351-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGTEEAILG GRDSHPAAGG GSVLCFGQCQ YTAEEYQAIQ KALRQRLGPE
60 70 80 90 100
YISSRMAGGG QKVCYIEGHR VINLANEMFG YNGWAHSITQ QNVDFVDLNN
110 120 130 140 150
GKFYVGVCAF VRVQLKDGSY HEDVGYGVSE GLKSKALSLE KARKEAVTDG
160 170 180 190 200
LKRALRSFGN ALGNCILDKD YLRSLNKLPR QLPLEVDLTK AKRQDLEPSV
210 220 230 240 250
EEARYNSCRP NMALGHPQLQ QVTSPSRPSH AVIPADQDCS SRSLSSSAVE
260 270 280 290 300
SEATHQRKLR QKQLQQQFRE RMEKQQVRVS TPSAEKSEAA PPAPPVTHST
310 320 330 340 350
PVTVSEPLLE KDFLAGVTQE LIKTLEDNSE KWAVTPDAGD GVVKPSSRAD
360 370 380 390 400
PAQTSDTLAL NNQMVTQNRT PHSVCHQKPQ AKSGSWDLQT YSADQRTTGN
410
WESHRKSQDM KKRKYDPS
Length:418
Mass (Da):46,169
Last modified:November 1, 1995 - v1
Checksum:i7E1BBCAF0B9CD4AF
GO
Isoform beta (identifier: P43351-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     157-226: SFGNALGNCI...PQLQQVTSPS → LPLLGVSGRI...SGQRLPEITK
     227-418: Missing.

Note: Unable to interact with isoform alpha, may act as dominant negative.
Show »
Length:226
Mass (Da):24,540
Checksum:i8B0E3FC5773B46FE
GO
Isoform gamma (identifier: P43351-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     117-139: DGSYHEDVGYGVSEGLKSKALSL → VRGWSRPAARKDQWVVGEGWFIS
     140-418: Missing.

Note: Unable to interact with isoform alpha, may act as dominant negative.
Show »
Length:139
Mass (Da):15,350
Checksum:iDFA2F7994CACC1DF
GO
Isoform delta (identifier: P43351-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     94-118: DFVDLNNGKFYVGVCAFVRVQLKDG → GEYALQQWGLLHCPAPAESLLWVRR
     119-418: Missing.

Note: Unable to interact with isoform alpha, may act as dominant negative.
Show »
Length:118
Mass (Da):12,943
Checksum:iC3341B828BE835A5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100N → K in AAA87554 (PubMed:7797537).Curated1
Sequence conflicti418S → SY in AAA87554 (PubMed:7797537).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01921870R → W.1 PublicationCorresponds to variant rs11571421dbSNPEnsembl.1
Natural variantiVAR_019219221Q → E.1 PublicationCorresponds to variant rs4987206dbSNPEnsembl.1
Natural variantiVAR_019220287S → N.1 PublicationCorresponds to variant rs11571463dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04774994 – 118DFVDL…QLKDG → GEYALQQWGLLHCPAPAESL LWVRR in isoform delta. 2 PublicationsAdd BLAST25
Alternative sequenceiVSP_047750117 – 139DGSYH…KALSL → VRGWSRPAARKDQWVVGEGW FIS in isoform gamma. 2 PublicationsAdd BLAST23
Alternative sequenceiVSP_047751119 – 418Missing in isoform delta. 2 PublicationsAdd BLAST300
Alternative sequenceiVSP_047752140 – 418Missing in isoform gamma. 2 PublicationsAdd BLAST279
Alternative sequenceiVSP_047753157 – 226SFGNA…VTSPS → LPLLGVSGRILYSLFSVHSV MCAGGLPTPTASAQTAPSSP CSSAVLRYAQEFWECTWKLY SGQRLPEITK in isoform beta. 1 PublicationAdd BLAST70
Alternative sequenceiVSP_047754227 – 418Missing in isoform beta. 1 PublicationAdd BLAST192

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12134 mRNA. Translation: AAA85793.1.
L33262 mRNA. Translation: AAB05203.1.
U27516 mRNA. Translation: AAA87554.1.
AF125948 mRNA. Translation: AAD24575.1.
AF125949 mRNA. Translation: AAD24576.1.
AF125950 mRNA. Translation: AAD24577.1.
AK292160 mRNA. Translation: BAF84849.1.
AK312026 mRNA. Translation: BAG34963.1.
AY527412 Genomic DNA. Translation: AAS00097.1.
AC004803 Genomic DNA. No translation available.
CH471116 Genomic DNA. Translation: EAW88943.1.
CH471116 Genomic DNA. Translation: EAW88945.1.
CH471116 Genomic DNA. Translation: EAW88946.1.
CH471116 Genomic DNA. Translation: EAW88949.1.
CCDSiCCDS76501.1. [P43351-2]
CCDS8507.2. [P43351-1]
PIRiA57518.
RefSeqiNP_001284348.1. NM_001297419.1. [P43351-1]
NP_001284349.1. NM_001297420.1. [P43351-2]
NP_602296.2. NM_134424.3. [P43351-1]
XP_005253777.1. XM_005253720.4. [P43351-1]
XP_005253778.1. XM_005253721.2. [P43351-1]
XP_011519292.1. XM_011520990.2. [P43351-1]
XP_011519293.1. XM_011520991.2. [P43351-1]
XP_016875258.1. XM_017019769.1. [P43351-1]
UniGeneiHs.410355.

Genome annotation databases

EnsembliENST00000358495; ENSP00000351284; ENSG00000002016. [P43351-1]
ENST00000430095; ENSP00000387901; ENSG00000002016. [P43351-1]
ENST00000461568; ENSP00000436008; ENSG00000002016. [P43351-3]
ENST00000468231; ENSP00000434703; ENSG00000002016. [P43351-3]
ENST00000541619; ENSP00000438965; ENSG00000002016. [P43351-4]
ENST00000544742; ENSP00000443254; ENSG00000002016. [P43351-4]
ENST00000545564; ENSP00000440268; ENSG00000002016. [P43351-2]
GeneIDi5893.
KEGGihsa:5893.
UCSCiuc001qiv.2. human. [P43351-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12134 mRNA. Translation: AAA85793.1.
L33262 mRNA. Translation: AAB05203.1.
U27516 mRNA. Translation: AAA87554.1.
AF125948 mRNA. Translation: AAD24575.1.
AF125949 mRNA. Translation: AAD24576.1.
AF125950 mRNA. Translation: AAD24577.1.
AK292160 mRNA. Translation: BAF84849.1.
AK312026 mRNA. Translation: BAG34963.1.
AY527412 Genomic DNA. Translation: AAS00097.1.
AC004803 Genomic DNA. No translation available.
CH471116 Genomic DNA. Translation: EAW88943.1.
CH471116 Genomic DNA. Translation: EAW88945.1.
CH471116 Genomic DNA. Translation: EAW88946.1.
CH471116 Genomic DNA. Translation: EAW88949.1.
CCDSiCCDS76501.1. [P43351-2]
CCDS8507.2. [P43351-1]
PIRiA57518.
RefSeqiNP_001284348.1. NM_001297419.1. [P43351-1]
NP_001284349.1. NM_001297420.1. [P43351-2]
NP_602296.2. NM_134424.3. [P43351-1]
XP_005253777.1. XM_005253720.4. [P43351-1]
XP_005253778.1. XM_005253721.2. [P43351-1]
XP_011519292.1. XM_011520990.2. [P43351-1]
XP_011519293.1. XM_011520991.2. [P43351-1]
XP_016875258.1. XM_017019769.1. [P43351-1]
UniGeneiHs.410355.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H2IX-ray2.70A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V1-209[»]
1KN0X-ray2.85A/B/C/D/E/F/G/H/I/J/K1-212[»]
5JRBX-ray2.40A/B/C/D/E/F/G/H/I/J/K1-212[»]
DisProtiDP00437.
ProteinModelPortaliP43351.
SMRiP43351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111830. 40 interactors.
DIPiDIP-333N.
IntActiP43351. 36 interactors.
MINTiMINT-2803258.
STRINGi9606.ENSP00000351284.

Chemistry databases

ChEMBLiCHEMBL2362978.

PTM databases

iPTMnetiP43351.
PhosphoSitePlusiP43351.

Polymorphism and mutation databases

BioMutaiRAD52.
DMDMi1172823.

Proteomic databases

EPDiP43351.
PaxDbiP43351.
PeptideAtlasiP43351.
PRIDEiP43351.

Protocols and materials databases

DNASUi5893.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358495; ENSP00000351284; ENSG00000002016. [P43351-1]
ENST00000430095; ENSP00000387901; ENSG00000002016. [P43351-1]
ENST00000461568; ENSP00000436008; ENSG00000002016. [P43351-3]
ENST00000468231; ENSP00000434703; ENSG00000002016. [P43351-3]
ENST00000541619; ENSP00000438965; ENSG00000002016. [P43351-4]
ENST00000544742; ENSP00000443254; ENSG00000002016. [P43351-4]
ENST00000545564; ENSP00000440268; ENSG00000002016. [P43351-2]
GeneIDi5893.
KEGGihsa:5893.
UCSCiuc001qiv.2. human. [P43351-1]

Organism-specific databases

CTDi5893.
DisGeNETi5893.
GeneCardsiRAD52.
HGNCiHGNC:9824. RAD52.
HPAiCAB072847.
CAB072848.
HPA046628.
MIMi600392. gene.
neXtProtiNX_P43351.
OpenTargetsiENSG00000002016.
PharmGKBiPA34180.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4141. Eukaryota.
COG5055. LUCA.
GeneTreeiENSGT00390000008766.
HOGENOMiHOG000237354.
HOVERGENiHBG054676.
InParanoidiP43351.
KOiK10873.
OMAiQARYSSC.
OrthoDBiEOG091G0OIP.
PhylomeDBiP43351.
TreeFamiTF101221.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000002016-MONOMER.
ReactomeiR-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-5685938. HDR through Single Strand Annealing (SSA).
SIGNORiP43351.

Miscellaneous databases

ChiTaRSiRAD52. human.
EvolutionaryTraceiP43351.
GeneWikiiRAD52.
GenomeRNAii5893.
PROiP43351.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000002016.
CleanExiHS_RAD52.
ExpressionAtlasiP43351. baseline and differential.
GenevisibleiP43351. HS.

Family and domain databases

InterProiIPR004585. DNA_recomb/repair_Rad52.
IPR007232. Rad52_Rad59_Rad22.
[Graphical view]
PANTHERiPTHR12132. PTHR12132. 1 hit.
PfamiPF04098. Rad52_Rad22. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00607. rad52. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRAD52_HUMAN
AccessioniPrimary (citable) accession number: P43351
Secondary accession number(s): Q13205
, Q9Y5T7, Q9Y5T8, Q9Y5T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.