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P43346

- DCK_MOUSE

UniProt

P43346 - DCK_MOUSE

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Protein

Deoxycytidine kinase

Gene

Dck

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA).

Catalytic activityi

NTP + deoxycytidine = NDP + dCMP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531SubstrateBy similarity
Binding sitei86 – 861SubstrateBy similarity
Binding sitei97 – 971SubstrateBy similarity
Active sitei127 – 1271Proton acceptorSequence Analysis
Binding sitei128 – 1281SubstrateBy similarity
Binding sitei133 – 1331SubstrateBy similarity
Binding sitei197 – 1971SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 369ATPBy similarity
Nucleotide bindingi188 – 1925ATPBy similarity
Nucleotide bindingi240 – 2423ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. deoxycytidine kinase activity Source: UniProtKB
  3. drug binding Source: Ensembl

GO - Biological processi

  1. deoxyribonucleoside monophosphate biosynthetic process Source: GOC
  2. pyrimidine nucleotide metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.74. 3474.
SABIO-RKP43346.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxycytidine kinase (EC:2.7.1.74)
Short name:
dCK
Gene namesi
Name:Dck
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:102726. Dck.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 260260Deoxycytidine kinasePRO_0000175091Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111Phosphoserine; by CK1By similarity
Modified residuei15 – 151Phosphoserine; by CK1By similarity
Modified residuei72 – 721Phosphothreonine; by CK1By similarity
Modified residuei74 – 741Phosphoserine; by CK1By similarity

Post-translational modificationi

Phosphorylated and activated in vitro upon phosphorylation at Ser-74 by CSNK1D/CK1.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43346.
PaxDbiP43346.
PRIDEiP43346.

PTM databases

PhosphoSiteiP43346.

Expressioni

Gene expression databases

BgeeiP43346.
CleanExiMM_DCK.
ExpressionAtlasiP43346. baseline and differential.
GenevestigatoriP43346.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP43346.
SMRiP43346. Positions 19-260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DCK/DGK family.Curated

Phylogenomic databases

eggNOGiCOG1428.
HOGENOMiHOG000290165.
HOVERGENiHBG006216.
InParanoidiP43346.
KOiK00893.
OMAiVNEDFKG.
OrthoDBiEOG70PBZM.
PhylomeDBiP43346.
TreeFamiTF324413.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10513. PTHR10513. 1 hit.
PfamiPF01712. dNK. 1 hit.
[Graphical view]
PIRSFiPIRSF000705. DNK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P43346-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATPPKRFCP SPSTSSEGTR IKKISIEGNI AAGKSTFVNI LKQASEDWEV
60 70 80 90 100
VPEPVARWCN VQSTQEEFEE LTTSQKSGGN VLQMMYEKPE RWSFTFQSYA
110 120 130 140 150
CLSRIRAQLA SLNGKLKDAE KPVLFFERSV YSDRYIFASN LYESDCMNET
160 170 180 190 200
EWTIYQDWHD WMNSQFGQSL ELDGIIYLRA TPEKCLNRIY LRGRNEEQGI
210 220 230 240 250
PLEYLEKLHY KHESWLLHRT LKTSFDYLQE VPVLTLDVNE DFKDKHESLV
260
EKVKEFLSTL
Length:260
Mass (Da):30,367
Last modified:November 1, 1995 - v1
Checksum:i778FDDDD07407BDE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77731 mRNA. Translation: CAA54787.1.
AF260315 Genomic DNA. Translation: AAG53743.1.
BC060062 mRNA. Translation: AAH60062.1.
CCDSiCCDS19405.1.
PIRiA55122.
RefSeqiNP_031858.1. NM_007832.4.
UniGeneiMm.298892.

Genome annotation databases

EnsembliENSMUST00000031311; ENSMUSP00000031311; ENSMUSG00000029366.
GeneIDi13178.
KEGGimmu:13178.
UCSCiuc008yah.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77731 mRNA. Translation: CAA54787.1 .
AF260315 Genomic DNA. Translation: AAG53743.1 .
BC060062 mRNA. Translation: AAH60062.1 .
CCDSi CCDS19405.1.
PIRi A55122.
RefSeqi NP_031858.1. NM_007832.4.
UniGenei Mm.298892.

3D structure databases

ProteinModelPortali P43346.
SMRi P43346. Positions 19-260.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P43346.
ChEMBLi CHEMBL2570.

PTM databases

PhosphoSitei P43346.

Proteomic databases

MaxQBi P43346.
PaxDbi P43346.
PRIDEi P43346.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000031311 ; ENSMUSP00000031311 ; ENSMUSG00000029366 .
GeneIDi 13178.
KEGGi mmu:13178.
UCSCi uc008yah.1. mouse.

Organism-specific databases

CTDi 1633.
MGIi MGI:102726. Dck.

Phylogenomic databases

eggNOGi COG1428.
HOGENOMi HOG000290165.
HOVERGENi HBG006216.
InParanoidi P43346.
KOi K00893.
OMAi VNEDFKG.
OrthoDBi EOG70PBZM.
PhylomeDBi P43346.
TreeFami TF324413.

Enzyme and pathway databases

BRENDAi 2.7.1.74. 3474.
SABIO-RK P43346.

Miscellaneous databases

NextBioi 283288.
PROi P43346.
SOURCEi Search...

Gene expression databases

Bgeei P43346.
CleanExi MM_DCK.
ExpressionAtlasi P43346. baseline and differential.
Genevestigatori P43346.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR10513. PTHR10513. 1 hit.
Pfami PF01712. dNK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000705. DNK. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "2 cloning and expression of mouse deoxycytidine kinase. Pure recombinant mouse and human enzymes show differences in substrate specificity."
    Karlsson A., Johansson M., Eriksson S.
    J. Biol. Chem. 269:24374-24378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Conserved gene structure and transcription factor sites in the human and mouse deoxycytidine kinase genes."
    Johansson M., Norda A., Karlsson A.
    FEBS Lett. 487:209-212(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiDCK_MOUSE
AccessioniPrimary (citable) accession number: P43346
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3