P43346 (DCK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxycytidine kinase
Short name=dCK
EC=2.7.1.74

Gene names

Name:

Dck

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA).
Catalytic activity	NTP + deoxycytidine = NDP + dCMP.
Subunit structure	Homodimer.
Subcellular location	Nucleus By similarity.
Post-translational modification	Phosphorylated and activated in vitro upon phosphorylation at Ser-74 by CSNK1D/CK1 By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.4 Ref.5
Sequence similarities	Belongs to the DCK/DGK family.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW deoxycytidine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB phosphotransferase activity, alcohol group as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Deoxycytidine kinase		PRO_0000175091
Regions
	Nucleotide binding	28 – 36	9	ATP By similarity
	Nucleotide binding	188 – 192	5	ATP By similarity
	Nucleotide binding	240 – 242	3	ATP By similarity
Sites
	Active site	127	1	Proton acceptor Potential
	Binding site	53	1	Substrate By similarity
	Binding site	86	1	Substrate By similarity
	Binding site	97	1	Substrate By similarity
	Binding site	128	1	Substrate By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	197	1	Substrate By similarity
Amino acid modifications
	Modified residue	3	1	Phosphothreonine By similarity
	Modified residue	11	1	Phosphoserine; by CK1 By similarity
	Modified residue	13	1	Phosphoserine By similarity
	Modified residue	15	1	Phosphoserine; by CK1 By similarity
	Modified residue	16	1	Phosphoserine By similarity
	Modified residue	35	1	Phosphoserine By similarity
	Modified residue	63	1	Phosphoserine By similarity
	Modified residue	72	1	Phosphothreonine; by CK1 By similarity
	Modified residue	74	1	Phosphoserine; by CK1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43346 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 778FDDDD07407BDE	FASTA	260	30,367
	10 20 30 40 50 60 MATPPKRFCP SPSTSSEGTR IKKISIEGNI AAGKSTFVNI LKQASEDWEV VPEPVARWCN 70 80 90 100 110 120 VQSTQEEFEE LTTSQKSGGN VLQMMYEKPE RWSFTFQSYA CLSRIRAQLA SLNGKLKDAE 130 140 150 160 170 180 KPVLFFERSV YSDRYIFASN LYESDCMNET EWTIYQDWHD WMNSQFGQSL ELDGIIYLRA 190 200 210 220 230 240 TPEKCLNRIY LRGRNEEQGI PLEYLEKLHY KHESWLLHRT LKTSFDYLQE VPVLTLDVNE 250 260 DFKDKHESLV EKVKEFLSTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"2 cloning and expression of mouse deoxycytidine kinase. Pure recombinant mouse and human enzymes show differences in substrate specificity." Karlsson A., Johansson M., Eriksson S. J. Biol. Chem. 269:24374-24378(1994) [PubMed: 7929097] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Conserved gene structure and transcription factor sites in the human and mouse deoxycytidine kinase genes." Johansson M., Norda A., Karlsson A. FEBS Lett. 487:209-212(2000) [PubMed: 11150511] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, MASS SPECTROMETRY. Tissue: Liver.
[5]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-74, MASS SPECTROMETRY. Tissue: Embryonic kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77731 mRNA. Translation: CAA54787.1. AF260315 Genomic DNA. Translation: AAG53743.1. BC060062 mRNA. Translation: AAH60062.1.
IPI	IPI00119261.
PIR	A55122.
RefSeq	NP_031858.1. NM_007832.4.
UniGene	Mm.298892.
3D structure databases
ProteinModelPortal	P43346.
SMR	P43346. Positions 19-260.
ModBase	Search...
Protein-protein interaction databases
STRING	P43346.
PTM databases
PhosphoSite	P43346.
Proteomic databases
PRIDE	P43346.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000031311; ENSMUSP00000031311; ENSMUSG00000029366.
GeneID	13178.
KEGG	mmu:13178.
Organism-specific databases
CTD	1633.
MGI	MGI:102726. Dck.
Phylogenomic databases
eggNOG	roNOG14466.
HOGENOM	HBG716740.
HOVERGEN	HBG006216.
InParanoid	P43346.
OMA	HRTLKTN.
OrthoDB	EOG48WC2T.
PhylomeDB	P43346.
Enzyme and pathway databases
BRENDA	2.7.1.74. 3474.
Gene expression databases
ArrayExpress	P43346.
Bgee	P43346.
CleanEx	MM_DCK.
Genevestigator	P43346.
GermOnline	ENSMUSG00000029366. Mus musculus.
Family and domain databases
InterPro	IPR002624. Deoxynucleoside_kinase. [Graphical view]
KO	K00893.
PANTHER	PTHR10513. dNK. 1 hit.
Pfam	PF01712. dNK. 1 hit. [Graphical view]
PIRSF	PIRSF000705. DNK. 1 hit.
ProtoNet	Search...
Other
NextBio	283288.
SOURCE	Search...

Entry information

Entry name

DCK_MOUSE

Accession

Primary (citable) accession number: P43346

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program