Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P43346 (DCK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxycytidine kinase

Short name=dCK
EC=2.7.1.74
Gene names
Name:Dck
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length260 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA).

Catalytic activity

NTP + deoxycytidine = NDP + dCMP.

Subunit structure

Homodimer.

Subcellular location

Nucleus By similarity.

Post-translational modification

Phosphorylated and activated in vitro upon phosphorylation at Ser-74 by CSNK1D/CK1 By similarity.

Sequence similarities

Belongs to the DCK/DGK family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 260260Deoxycytidine kinase
PRO_0000175091

Regions

Nucleotide binding28 – 369ATP By similarity
Nucleotide binding188 – 1925ATP By similarity
Nucleotide binding240 – 2423ATP By similarity

Sites

Active site1271Proton acceptor Potential
Binding site531Substrate By similarity
Binding site861Substrate By similarity
Binding site971Substrate By similarity
Binding site1281Substrate By similarity
Binding site1331Substrate By similarity
Binding site1971Substrate By similarity

Amino acid modifications

Modified residue111Phosphoserine; by CK1 By similarity
Modified residue151Phosphoserine; by CK1 By similarity
Modified residue721Phosphothreonine; by CK1 By similarity
Modified residue741Phosphoserine; by CK1 By similarity

Sequences

Sequence LengthMass (Da)Tools
P43346 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 778FDDDD07407BDE

FASTA26030,367
        10         20         30         40         50         60 
MATPPKRFCP SPSTSSEGTR IKKISIEGNI AAGKSTFVNI LKQASEDWEV VPEPVARWCN 

        70         80         90        100        110        120 
VQSTQEEFEE LTTSQKSGGN VLQMMYEKPE RWSFTFQSYA CLSRIRAQLA SLNGKLKDAE 

       130        140        150        160        170        180 
KPVLFFERSV YSDRYIFASN LYESDCMNET EWTIYQDWHD WMNSQFGQSL ELDGIIYLRA 

       190        200        210        220        230        240 
TPEKCLNRIY LRGRNEEQGI PLEYLEKLHY KHESWLLHRT LKTSFDYLQE VPVLTLDVNE 

       250        260 
DFKDKHESLV EKVKEFLSTL 

« Hide

References

« Hide 'large scale' references
[1]"2 cloning and expression of mouse deoxycytidine kinase. Pure recombinant mouse and human enzymes show differences in substrate specificity."
Karlsson A., Johansson M., Eriksson S.
J. Biol. Chem. 269:24374-24378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Conserved gene structure and transcription factor sites in the human and mouse deoxycytidine kinase genes."
Johansson M., Norda A., Karlsson A.
FEBS Lett. 487:209-212(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ.
Tissue: Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77731 mRNA. Translation: CAA54787.1.
AF260315 Genomic DNA. Translation: AAG53743.1.
BC060062 mRNA. Translation: AAH60062.1.
CCDSCCDS19405.1.
PIRA55122.
RefSeqNP_031858.1. NM_007832.4.
UniGeneMm.298892.

3D structure databases

ProteinModelPortalP43346.
SMRP43346. Positions 19-260.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP43346.
ChEMBLCHEMBL2570.

PTM databases

PhosphoSiteP43346.

Proteomic databases

MaxQBP43346.
PaxDbP43346.
PRIDEP43346.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000031311; ENSMUSP00000031311; ENSMUSG00000029366.
GeneID13178.
KEGGmmu:13178.
UCSCuc008yah.1. mouse.

Organism-specific databases

CTD1633.
MGIMGI:102726. Dck.

Phylogenomic databases

eggNOGCOG1428.
HOGENOMHOG000290165.
HOVERGENHBG006216.
InParanoidP43346.
KOK00893.
OMAVNEDFKG.
OrthoDBEOG70PBZM.
PhylomeDBP43346.
TreeFamTF324413.

Enzyme and pathway databases

BRENDA2.7.1.74. 3474.
SABIO-RKP43346.

Gene expression databases

ArrayExpressP43346.
BgeeP43346.
CleanExMM_DCK.
GenevestigatorP43346.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR002624. Deoxynucleoside_kinase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR10513. PTHR10513. 1 hit.
PfamPF01712. dNK. 1 hit.
[Graphical view]
PIRSFPIRSF000705. DNK. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

NextBio283288.
PROP43346.
SOURCESearch...

Entry information

Entry nameDCK_MOUSE
AccessionPrimary (citable) accession number: P43346
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot