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P43346

- DCK_MOUSE

UniProt

P43346 - DCK_MOUSE

Protein

Deoxycytidine kinase

Gene

Dck

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA).

    Catalytic activityi

    NTP + deoxycytidine = NDP + dCMP.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei53 – 531SubstrateBy similarity
    Binding sitei86 – 861SubstrateBy similarity
    Binding sitei97 – 971SubstrateBy similarity
    Active sitei127 – 1271Proton acceptorSequence Analysis
    Binding sitei128 – 1281SubstrateBy similarity
    Binding sitei133 – 1331SubstrateBy similarity
    Binding sitei197 – 1971SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi28 – 369ATPBy similarity
    Nucleotide bindingi188 – 1925ATPBy similarity
    Nucleotide bindingi240 – 2423ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. deoxycytidine kinase activity Source: UniProtKB
    3. drug binding Source: Ensembl
    4. phosphotransferase activity, alcohol group as acceptor Source: InterPro

    GO - Biological processi

    1. deoxyribonucleoside monophosphate biosynthetic process Source: GOC
    2. pyrimidine nucleotide metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.1.74. 3474.
    SABIO-RKP43346.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxycytidine kinase (EC:2.7.1.74)
    Short name:
    dCK
    Gene namesi
    Name:Dck
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:102726. Dck.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 260260Deoxycytidine kinasePRO_0000175091Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei11 – 111Phosphoserine; by CK1By similarity
    Modified residuei15 – 151Phosphoserine; by CK1By similarity
    Modified residuei72 – 721Phosphothreonine; by CK1By similarity
    Modified residuei74 – 741Phosphoserine; by CK1By similarity

    Post-translational modificationi

    Phosphorylated and activated in vitro upon phosphorylation at Ser-74 by CSNK1D/CK1.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP43346.
    PaxDbiP43346.
    PRIDEiP43346.

    PTM databases

    PhosphoSiteiP43346.

    Expressioni

    Gene expression databases

    ArrayExpressiP43346.
    BgeeiP43346.
    CleanExiMM_DCK.
    GenevestigatoriP43346.

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP43346.
    SMRiP43346. Positions 19-260.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DCK/DGK family.Curated

    Phylogenomic databases

    eggNOGiCOG1428.
    HOGENOMiHOG000290165.
    HOVERGENiHBG006216.
    InParanoidiP43346.
    KOiK00893.
    OMAiVNEDFKG.
    OrthoDBiEOG70PBZM.
    PhylomeDBiP43346.
    TreeFamiTF324413.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR002624. DNK.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10513. PTHR10513. 1 hit.
    PfamiPF01712. dNK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000705. DNK. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P43346-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATPPKRFCP SPSTSSEGTR IKKISIEGNI AAGKSTFVNI LKQASEDWEV    50
    VPEPVARWCN VQSTQEEFEE LTTSQKSGGN VLQMMYEKPE RWSFTFQSYA 100
    CLSRIRAQLA SLNGKLKDAE KPVLFFERSV YSDRYIFASN LYESDCMNET 150
    EWTIYQDWHD WMNSQFGQSL ELDGIIYLRA TPEKCLNRIY LRGRNEEQGI 200
    PLEYLEKLHY KHESWLLHRT LKTSFDYLQE VPVLTLDVNE DFKDKHESLV 250
    EKVKEFLSTL 260
    Length:260
    Mass (Da):30,367
    Last modified:November 1, 1995 - v1
    Checksum:i778FDDDD07407BDE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77731 mRNA. Translation: CAA54787.1.
    AF260315 Genomic DNA. Translation: AAG53743.1.
    BC060062 mRNA. Translation: AAH60062.1.
    CCDSiCCDS19405.1.
    PIRiA55122.
    RefSeqiNP_031858.1. NM_007832.4.
    UniGeneiMm.298892.

    Genome annotation databases

    EnsembliENSMUST00000031311; ENSMUSP00000031311; ENSMUSG00000029366.
    GeneIDi13178.
    KEGGimmu:13178.
    UCSCiuc008yah.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77731 mRNA. Translation: CAA54787.1 .
    AF260315 Genomic DNA. Translation: AAG53743.1 .
    BC060062 mRNA. Translation: AAH60062.1 .
    CCDSi CCDS19405.1.
    PIRi A55122.
    RefSeqi NP_031858.1. NM_007832.4.
    UniGenei Mm.298892.

    3D structure databases

    ProteinModelPortali P43346.
    SMRi P43346. Positions 19-260.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P43346.
    ChEMBLi CHEMBL2570.

    PTM databases

    PhosphoSitei P43346.

    Proteomic databases

    MaxQBi P43346.
    PaxDbi P43346.
    PRIDEi P43346.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031311 ; ENSMUSP00000031311 ; ENSMUSG00000029366 .
    GeneIDi 13178.
    KEGGi mmu:13178.
    UCSCi uc008yah.1. mouse.

    Organism-specific databases

    CTDi 1633.
    MGIi MGI:102726. Dck.

    Phylogenomic databases

    eggNOGi COG1428.
    HOGENOMi HOG000290165.
    HOVERGENi HBG006216.
    InParanoidi P43346.
    KOi K00893.
    OMAi VNEDFKG.
    OrthoDBi EOG70PBZM.
    PhylomeDBi P43346.
    TreeFami TF324413.

    Enzyme and pathway databases

    BRENDAi 2.7.1.74. 3474.
    SABIO-RK P43346.

    Miscellaneous databases

    NextBioi 283288.
    PROi P43346.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P43346.
    Bgeei P43346.
    CleanExi MM_DCK.
    Genevestigatori P43346.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR002624. DNK.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10513. PTHR10513. 1 hit.
    Pfami PF01712. dNK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000705. DNK. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "2 cloning and expression of mouse deoxycytidine kinase. Pure recombinant mouse and human enzymes show differences in substrate specificity."
      Karlsson A., Johansson M., Eriksson S.
      J. Biol. Chem. 269:24374-24378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Conserved gene structure and transcription factor sites in the human and mouse deoxycytidine kinase genes."
      Johansson M., Norda A., Karlsson A.
      FEBS Lett. 487:209-212(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ.
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiDCK_MOUSE
    AccessioniPrimary (citable) accession number: P43346
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3