P43336 (PHHC_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aromatic-amino-acid aminotransferase
EC=2.6.1.57

Gene names

Name:	phhC
Ordered Locus Names:	PA0870

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	An aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-phenylalanine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC aromatic-amino-acid:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Aromatic-amino-acid aminotransferase		PRO_0000123895
Amino acid modifications
	Modified residue	247	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	382	1	D → H in AAA25938. Ref.1
	Sequence conflict	387	1	D → G in AAA25938. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P43336 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: B6162FE13EBDB6E1	FASTA	399	43,273
	10 20 30 40 50 60 MSHFAKVARV PGDPILGLLD AYRNDPRADK LDLGVGVYKD AQGLTPILRS VKLAEQRLVE 70 80 90 100 110 120 QETTKSYVGG HGDALFAARL AELALGAASP LLLEQRADAT QTPGGTGALR LAGDFIAHCL 130 140 150 160 170 180 PGRGIWLSDP TWPIHETLFA AAGLKVSHYP YVSADNRLDV EAMLAGLERI PQGDVVLLHA 190 200 210 220 230 240 CCHNPTGFDL SHDDWRRVLD VVRRRELLPL IDFAYQGFGD GLEEDAWAVR LFAGELPEVL 250 260 270 280 290 300 VTSSCSKNFG LYRDRVGALI VCAQNAEKLT DLRSQLAFLA RNLWSTPPAH GAEVVAAILG 310 320 330 340 350 360 DSELKGLWQE EVEGMRSRIA SLRIGLVEAL APHGLAERFA HVGAQRGMFS YTGLSPQQVA 370 380 390 RLRDEHAVYL VSSGRANVAG LDARRLDRLA QAIAQVCAD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pseudomonas aeruginosa possesses homologues of mammalian phenylalanine hydroxylase and 4 alpha-carbinolamine dehydratase/DCoH as part of a three-component gene cluster." Zhao G., Xia T., Song J., Roy R.A. Proc. Natl. Acad. Sci. U.S.A. 91:1366-1370(1994) [PubMed: 8108417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M88627 Genomic DNA. Translation: AAA25938.1. AE004091 Genomic DNA. Translation: AAG04259.1.
PIR	D83535.
RefSeq	NP_249561.1. NC_002516.2.
3D structure databases
ProteinModelPortal	P43336.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	880839.
GenomeReviews	Gene locus PA0870 in contig AE004091_GR.
KEGG	pae:PA0870.
NMPDR	fig|208964.1.peg.871.
PATRIC	19836024. VBIPseAer58763_0904.
Organism-specific databases
PseudoCAP	PA0870.
Phylogenomic databases
HOGENOM	HBG446828.
OMA	FLARNLW.
ProtClustDB	PRK09257.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12023. PAER208964:PA0870-MONOMER.
Family and domain databases
InterPro	IPR004839. Aminotransferase_I/II. IPR000796. Asp_trans. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
KO	K00832.
PANTHER	PTHR11879. Asp_trans. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00799. TRANSAMINASE.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHHC_PSEAE

Accession

Primary (citable) accession number: P43336

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	December 8, 2000
Last modified:	January 25, 2012
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program