Reviewed, UniProtKB/Swiss-Prot P43335 (PHS_PSEAE)

Names and origin

Protein names

Recommended name:
    Pterin-4-alpha-carbinolamine dehydratase
      Short name=PHS
    EC=4.2.1.96
Alternative name(s):
    4-alpha-hydroxy-tetrahydropterin dehydratase
    Pterin carbinolamine dehydratase
      Short name=PCD

Gene names

Name:	phhB
Ordered Locus Names:	PA0871

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. May also have a positive regulatory role in the expression of phhA. HAMAP MF_00434
Catalytic activity	(6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin = (6R)-6-(L-erythro-1,2-dihydroxypropyl)-7,8-dihydro-6H-pterin + H2O. HAMAP MF_00434
Subunit structure	Homodimer Probable.
Sequence similarities	Belongs to the pterin-4-alpha-carbinolamine dehydratase family.

Ontologies

Keywords
Biological process	Tetrahydrobiopterin biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tetrahydrobiopterin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-alpha-hydroxytetrahydrobiopterin dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	Pterin-4-alpha-carbinolamine dehydratase HAMAP MF_00434		PRO_0000063065

Sequences

	Sequence		Length	Mass (Da)
	P43335-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 02502CBC1F9ADF94	FASTA	118	13,333
	10 20 30 40 50 60 MTALTQAHCE ACRADAPHVS DEELPVLLRQ IPDWNIEVRD GIMQLEKVYL FKNFKHALAF 70 80 90 100 110 TNAVGEISEA EGHHPGLLTE WGKVTVTWWS HSIKGLHRND FIMAARTDEV AKTAEGRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pseudomonas aeruginosa possesses homologues of mammalian phenylalanine hydroxylase and 4 alpha-carbinolamine dehydratase/DCoH as part of a three-component gene cluster." Zhao G., Xia T., Song J., Roy R.A. Proc. Natl. Acad. Sci. U.S.A. 91:1366-1370(1994) [PubMed: 8108417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M88627 Genomic DNA. Translation: AAA25937.1. AE004091 Genomic DNA. Translation: AAG04260.1.
PIR	E83535.
RefSeq	NP_249562.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	880827.
GenomeReviews	Gene locus PA0871 in contig AE004091_GR.
KEGG	pae:PA0871.
Organism-specific databases
PseudoCAP	PA0871.
CMR	Search...
Phylogenomic databases
HOGENOM	P43335.
OMA	ILTEWGK.
Enzyme and pathway databases
BioCyc	PAER208964:PA0871-MON.
BRENDA	4.2.1.96. 354.
Family and domain databases
HAMAP	MF_00434. [Tree]
InterPro	IPR001533. Trans/pterin_deHydtase. [Graphical view]
Gene3D	G3DSA:3.30.1360.20. Trans_pterinDh. 1 hit.
PANTHER	PTHR12599. Trans_pterinDh. 1 hit.
Pfam	PF01329. Pterin_4a. 1 hit. [Graphical view]
ProDom	PD007262. Trans_pterinDh. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

PHS_PSEAE

Accession

Primary (citable) accession number: P43335

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)