Reviewed, UniProtKB/Swiss-Prot P43334 (PH4H_PSEAE)

Names and origin

Protein names

Recommended name:
    Phenylalanine-4-hydroxylase
      Short name=PAH
    EC=1.14.16.1
Alternative name(s):
    Phe-4-monooxygenase

Gene names

Name:	phhA
Ordered Locus Names:	PA0872

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-phenylalanine + tetrahydrobiopterin + O(2) = L-tyrosine + 4a-hydroxytetrahydrobiopterin.
Cofactor	Binds 1 Fe(2+) ion.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 1/6.
Subunit structure	Monomer.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: InterPro phenylalanine 4-monooxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Phenylalanine-4-hydroxylase		PRO_0000205555
Sites
	Metal binding	121	1	Iron By similarity
	Metal binding	126	1	Iron By similarity
	Metal binding	166	1	Iron Potential
Experimental info
	Sequence conflict	135	1	F → L in AAA25936. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P43334-1 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: A5665839C5961A45	FASTA	262	30,322
	10 20 30 40 50 60 MKTTQYVARQ PDDNGFIHYP ETEHQVWNTL ITRQLKVIEG RACQEYLDGI EQLGLPHERI 70 80 90 100 110 120 PQLDEINRVL QATTGWRVAR VPALIPFQTF FELLASQQFP VATFIRTPEE LDYLQEPDIF 130 140 150 160 170 180 HEIFGHCPLL TNPWFAEFTH TYGKLGLKAS KEERVFLARL YWMTIEFGLV ETDQGKRIYG 190 200 210 220 230 240 GGILSSPKET VYSLSDEPLH QAFNPLEAMR TPYRIDILQP LYFVLPDLKR LFQLAQEDIM 250 260 ALVHEAMRLG LHAPLFPPKQ AA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pseudomonas aeruginosa possesses homologues of mammalian phenylalanine hydroxylase and 4 alpha-carbinolamine dehydratase/DCoH as part of a three-component gene cluster." Zhao G., Xia T., Song J., Roy R.A. Proc. Natl. Acad. Sci. U.S.A. 91:1366-1370(1994) [PubMed: 8108417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M88627 Genomic DNA. Translation: AAA25936.1. AE004091 Genomic DNA. Translation: AAG04261.1.
PIR	A53452. F83535.
RefSeq	NP_249563.1.
3D structure databases
HSSP	HSSP built from PDB template 1LTZ based on UniProtKB P30967.
ModBase	Search...
Genome annotation databases
GeneID	879709.
GenomeReviews	Gene locus PA0872 in contig AE004091_GR.
KEGG	pae:PA0872.
Organism-specific databases
PseudoCAP	PA0872.
CMR	Search...
Phylogenomic databases
HOGENOM	P43334.
Enzyme and pathway databases
BioCyc	PAER208964:PA0872-MON.
Family and domain databases
InterPro	IPR001273. Aaa_hydroxylase. IPR005960. Phe-4-hydroxylase_mono. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF00351. Biopterin_H. 1 hit. [Graphical view]
PRINTS	PR00372. FYWHYDRXLASE.
ProDom	PD002559. Aaa_hydroxylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01267. Phe4hydrox_mono. 1 hit.
PROSITE	PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PH4H_PSEAE

Accession

Primary (citable) accession number: P43334

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	December 8, 2000
Last modified:	November 25, 2008
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)