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Protein

U1 small nuclear ribonucleoprotein A

Gene

snf

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP (By similarity). Plays a role in regulating sex-lethal splicing.By similarity1 Publication

GO - Molecular functioni

  • identical protein binding Source: FlyBase
  • mRNA binding Source: FlyBase
  • poly(U) RNA binding Source: FlyBase
  • pre-mRNA intronic pyrimidine-rich binding Source: FlyBase
  • snRNA stem-loop binding Source: FlyBase
  • U1 snRNA binding Source: FlyBase
  • U2 snRNA binding Source: FlyBase

GO - Biological processi

  • female germ-line sex determination Source: FlyBase
  • heterochromatin organization involved in chromatin silencing Source: FlyBase
  • mRNA splicing, via spliceosome Source: FlyBase
  • oogenesis Source: FlyBase
  • primary sex determination, soma Source: FlyBase
  • regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
  • RNA splicing Source: FlyBase

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-DME-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
U1 small nuclear ribonucleoprotein A
Short name:
U1 snRNP A
Short name:
U1-A
Short name:
U1A
Alternative name(s):
Sex determination protein snf
Gene namesi
Name:snf
Synonyms:D25, fs(1)1621, liz
ORF Names:CG4528
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0003449. snf.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000818901 – 216U1 small nuclear ribonucleoprotein AAdd BLAST216

Proteomic databases

PaxDbiP43332.
PRIDEiP43332.

Expressioni

Gene expression databases

BgeeiFBgn0003449.
ExpressionAtlasiP43332. differential.
GenevisibleiP43332. DM.

Interactioni

Subunit structurei

Belongs to the spliceosome where it is associated with snRNP U1. Interacts with the SMN complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
l(2)35BcQ9V3N83EBI-174177,EBI-131989

GO - Molecular functioni

  • identical protein binding Source: FlyBase

Protein-protein interaction databases

BioGridi57952. 39 interactors.
DIPiDIP-23676N.
IntActiP43332. 5 interactors.
MINTiMINT-1634151.
STRINGi7227.FBpp0070716.

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Helixi20 – 30Combined sources11
Helixi31 – 34Combined sources4
Beta strandi37 – 41Combined sources5
Helixi46 – 48Combined sources3
Beta strandi52 – 58Combined sources7
Helixi59 – 69Combined sources11
Beta strandi73 – 78Combined sources6
Beta strandi81 – 87Combined sources7
Helixi88 – 95Combined sources8
Beta strandi142 – 148Combined sources7
Helixi155 – 162Combined sources8
Beta strandi168 – 173Combined sources6
Turni175 – 177Combined sources3
Beta strandi179 – 186Combined sources8
Helixi188 – 198Combined sources11
Beta strandi201 – 204Combined sources4
Beta strandi208 – 213Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AYMNMR-A134-216[»]
2B0GNMR-A134-216[»]
2K3KNMR-A1-98[»]
ProteinModelPortaliP43332.
SMRiP43332.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43332.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 86RRM 1PROSITE-ProRule annotationAdd BLAST80
Domaini142 – 216RRM 2PROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Belongs to the RRM U1 A/B'' family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4206. Eukaryota.
ENOG410XPZI. LUCA.
GeneTreeiENSGT00390000007046.
InParanoidiP43332.
OMAiALHNYKL.
OrthoDBiEOG091G0NFU.
PhylomeDBiP43332.

Family and domain databases

InterProiView protein in InterPro
IPR000504. RRM_dom.
PfamiView protein in Pfam
PF00076. RRM_1. 2 hits.
SMARTiView protein in SMART
SM00360. RRM. 2 hits.
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiView protein in PROSITE
PS50102. RRM. 2 hits.

Sequencei

Sequence statusi: Complete.

P43332-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMLPNQTIY INNLNEKIKK EELKKSLYAI FSQFGQILDI VALKTLKMRG
60 70 80 90 100
QAFVIFKEIG SASNALRTMQ GFPFYDKPMQ IAYSKSDSDI VAKIKGTFKE
110 120 130 140 150
RPKKVKPPKP APGTDEKKDK KKKPSSAENS NPNAQTEQPP NQILFLTNLP
160 170 180 190 200
EETNEMMLSM LFNQFPGFKE VRLVPNRHDI AFVEFTTELQ SNAAKEALQG
210
FKITPTHAMK ITFAKK
Length:216
Mass (Da):24,547
Last modified:November 1, 1995 - v1
Checksum:i5B736FFE36523373
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti49R → H in allele SNF1621; sterile. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89775 mRNA. Translation: AAA28441.1.
L29521 Genomic DNA. Translation: AAA28903.1.
AE014298 Genomic DNA. Translation: AAF46017.1.
AY061491 mRNA. Translation: AAL29039.1.
PIRiA54279.
RefSeqiNP_511045.1. NM_078490.4.
UniGeneiDm.2625.

Genome annotation databases

EnsemblMetazoaiFBtr0070748; FBpp0070716; FBgn0003449.
GeneIDi31442.
KEGGidme:Dmel_CG4528.

Similar proteinsi

Entry informationi

Entry nameiSNRPA_DROME
AccessioniPrimary (citable) accession number: P43332
Secondary accession number(s): Q9W4D7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 27, 2017
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families