P43332 (SNRPA_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
U1 small nuclear ribonucleoprotein A
Short name=U1 snRNP A
Short name=U1-A
Short name=U1A

Alternative name(s):
Sex determination protein snf

Gene names

Name:	snf
Synonyms:	D25, fs(1)1621, liz
ORF Names:	CG4528

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP By similarity. Plays a role in regulating sex-lethal splicing.
Subunit structure	Belongs to the spliceosome where it is associated with snRNP U1.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the RRM U1 A/B'' family. Contains 2 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Domain	Repeat
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	female germ-line sex determination Non-traceable author statement. Source: FlyBase nuclear mRNA splicing, via spliceosome Inferred by curator. Source: FlyBase oogenesis Inferred from mutant phenotype. Source: FlyBase primary sex determination, soma Non-traceable author statement. Source: FlyBase regulation of alternative nuclear mRNA splicing, via spliceosome Inferred from mutant phenotype. Source: FlyBase
Cellular component	catalytic step 2 spliceosome Inferred from direct assay. Source: FlyBase precatalytic spliceosome Inferred from direct assay. Source: FlyBase protein complex Inferred from physical interaction. Source: FlyBase small nuclear ribonucleoprotein complex Inferred from direct assay. Source: FlyBase
Molecular function	U1 snRNA binding Inferred from direct assay. Source: FlyBase U2 snRNA binding Inferred from direct assay. Source: FlyBase nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: FlyBase snRNA stem-loop binding Inferred from direct assay. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 216

216

U1 small nuclear ribonucleoprotein A

PRO_0000081890

Regions

Domain

7 – 86

80

RRM 1

Domain

142 – 216

75

RRM 2

Natural variations

Natural variant

49

1

R → H in allele SNF1621; sterile.

Secondary structure

1

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216

Helix Strand Turn

Details...

Beta strand

7 – 11

5

Helix

20 – 30

11

Helix

31 – 34

4

Beta strand

37 – 41

5

Helix

46 – 48

3

Beta strand

52 – 58

7

Helix

59 – 69

11

Beta strand

73 – 78

6

Beta strand

81 – 87

7

Helix

88 – 95

8

Turn

97 – 99

3

Beta strand

142 – 148

7

Helix

155 – 162

8

Beta strand

168 – 173

6

Turn

175 – 177

3

Beta strand

179 – 186

8

Helix

188 – 198

11

Beta strand

201 – 204

4

Beta strand

208 – 213

6

Sequences

	Sequence		Length	Mass (Da)
	P43332 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 5B736FFE36523373	FASTA	216	24,547
	10 20 30 40 50 60 MEMLPNQTIY INNLNEKIKK EELKKSLYAI FSQFGQILDI VALKTLKMRG QAFVIFKEIG 70 80 90 100 110 120 SASNALRTMQ GFPFYDKPMQ IAYSKSDSDI VAKIKGTFKE RPKKVKPPKP APGTDEKKDK 130 140 150 160 170 180 KKKPSSAENS NPNAQTEQPP NQILFLTNLP EETNEMMLSM LFNQFPGFKE VRLVPNRHDI 190 200 210 AFVEFTTELQ SNAAKEALQG FKITPTHAMK ITFAKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RNA binding specificity of a Drosophila snRNP protein that shares sequence homology with mammalian U1-A and U2-B' proteins." Harper D.S., Fresco L.D., Keene J.D. Nucleic Acids Res. 20:3645-3650(1992) [PubMed: 1386424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The Drosophila sex determination gene snf encodes a nuclear protein with sequence and functional similarity to the mammalian U1A snRNP protein." Flickinger T.W., Salz H.K. Genes Dev. 8:914-925(1994) [PubMed: 7926776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: Oregon-R.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M89775 mRNA. Translation: AAA28441.1.
L29521 Genomic DNA. Translation: AAA28903.1.
AE014298 Genomic DNA. Translation: AAF46017.1.
AY061491 mRNA. Translation: AAL29039.1.

PIR

A54279.

RefSeq

NP_511045.1. NM_078490.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2AYM	NMR	-	A	134-216	[»]
2B0G	NMR	-	A	134-216	[»]
2K3K	NMR	-	A	1-104	[»]

ProteinModelPortal

P43332.

SMR

P43332. Positions 1-104, 134-216.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-23676N.

IntAct

P43332. 1 interaction.

MINT

MINT-1634151.

STRING

P43332.

Proteomic databases

PRIDE

P43332.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0070748; FBpp0070716; FBgn0003449.

GeneID

31442.

KEGG

dme:Dmel_CG4528.

NMPDR

fig|7227.3.peg.16553.

Organism-specific databases

CTD

31442.

FlyBase

FBgn0003449. snf.

Phylogenomic databases

eggNOG

inNOG04689.

GeneTree

EMGT00050000010959.

InParanoid

P43332.

OMA

APPNKIL.

OrthoDB

EOG4TMPJ8.

PhylomeDB

P43332.

Gene expression databases

Bgee

P43332.

GermOnline

CG4528. Drosophila melanogaster.

Family and domain databases

InterPro

IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR024888. U1_snRNP_A/U2_snRNP_B''.
[Graphical view]

Gene3D

G3DSA:3.30.70.330. a_b_plait_nuc_bd. 2 hits.

PANTHER

PTHR10501. PTHR10501. 1 hit.

Pfam

PF00076. RRM_1. 2 hits.
[Graphical view]

SMART

SM00360. RRM. 2 hits.
[Graphical view]

PROSITE

PS50102. RRM. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

773664.

Entry information

Entry name

SNRPA_DROME

Accession

Primary (citable) accession number: P43332
Secondary accession number(s): Q9W4D7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project