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Protein

U1 small nuclear ribonucleoprotein A

Gene

snf

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP (By similarity). Plays a role in regulating sex-lethal splicing.By similarity1 Publication

GO - Molecular functioni

  • identical protein binding Source: FlyBase
  • mRNA binding Source: FlyBase
  • nucleotide binding Source: InterPro
  • poly(U) RNA binding Source: FlyBase
  • pre-mRNA intronic pyrimidine-rich binding Source: FlyBase
  • snRNA stem-loop binding Source: FlyBase
  • U1 snRNA binding Source: FlyBase
  • U2 snRNA binding Source: FlyBase

GO - Biological processi

  • female germ-line sex determination Source: FlyBase
  • mRNA splicing, via spliceosome Source: FlyBase
  • oogenesis Source: FlyBase
  • primary sex determination, soma Source: FlyBase
  • regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
  • RNA splicing Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-DME-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
U1 small nuclear ribonucleoprotein A
Short name:
U1 snRNP A
Short name:
U1-A
Short name:
U1A
Alternative name(s):
Sex determination protein snf
Gene namesi
Name:snf
Synonyms:D25, fs(1)1621, liz
ORF Names:CG4528
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0003449. snf.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: FlyBase
  • intracellular ribonucleoprotein complex Source: FlyBase
  • nucleus Source: FlyBase
  • precatalytic spliceosome Source: FlyBase
  • protein complex Source: FlyBase
  • small nuclear ribonucleoprotein complex Source: FlyBase
  • spliceosomal complex Source: FlyBase
  • U1 snRNP Source: FlyBase
  • U2 snRNP Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000818901 – 216U1 small nuclear ribonucleoprotein AAdd BLAST216

Proteomic databases

PaxDbiP43332.
PRIDEiP43332.

Expressioni

Gene expression databases

BgeeiFBgn0003449.
GenevisibleiP43332. DM.

Interactioni

Subunit structurei

Belongs to the spliceosome where it is associated with snRNP U1. Interacts with the SMN complex.1 Publication

GO - Molecular functioni

  • identical protein binding Source: FlyBase

Protein-protein interaction databases

BioGridi57952. 9 interactors.
DIPiDIP-23676N.
IntActiP43332. 2 interactors.
MINTiMINT-1634151.
STRINGi7227.FBpp0070716.

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Helixi20 – 30Combined sources11
Helixi31 – 34Combined sources4
Beta strandi37 – 41Combined sources5
Helixi46 – 48Combined sources3
Beta strandi52 – 58Combined sources7
Helixi59 – 69Combined sources11
Beta strandi73 – 78Combined sources6
Beta strandi81 – 87Combined sources7
Helixi88 – 95Combined sources8
Beta strandi142 – 148Combined sources7
Helixi155 – 162Combined sources8
Beta strandi168 – 173Combined sources6
Turni175 – 177Combined sources3
Beta strandi179 – 186Combined sources8
Helixi188 – 198Combined sources11
Beta strandi201 – 204Combined sources4
Beta strandi208 – 213Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AYMNMR-A134-216[»]
2B0GNMR-A134-216[»]
2K3KNMR-A1-98[»]
ProteinModelPortaliP43332.
SMRiP43332.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43332.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 86RRM 1PROSITE-ProRule annotationAdd BLAST80
Domaini142 – 216RRM 2PROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Belongs to the RRM U1 A/B'' family.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4206. Eukaryota.
ENOG410XPZI. LUCA.
GeneTreeiENSGT00390000007046.
InParanoidiP43332.
OMAiESANEAR.
OrthoDBiEOG091G0NFU.
PhylomeDBiP43332.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR024888. RRM_U1_A/B''.
[Graphical view]
PANTHERiPTHR10501. PTHR10501. 1 hit.
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43332-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMLPNQTIY INNLNEKIKK EELKKSLYAI FSQFGQILDI VALKTLKMRG
60 70 80 90 100
QAFVIFKEIG SASNALRTMQ GFPFYDKPMQ IAYSKSDSDI VAKIKGTFKE
110 120 130 140 150
RPKKVKPPKP APGTDEKKDK KKKPSSAENS NPNAQTEQPP NQILFLTNLP
160 170 180 190 200
EETNEMMLSM LFNQFPGFKE VRLVPNRHDI AFVEFTTELQ SNAAKEALQG
210
FKITPTHAMK ITFAKK
Length:216
Mass (Da):24,547
Last modified:November 1, 1995 - v1
Checksum:i5B736FFE36523373
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti49R → H in allele SNF1621; sterile. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89775 mRNA. Translation: AAA28441.1.
L29521 Genomic DNA. Translation: AAA28903.1.
AE014298 Genomic DNA. Translation: AAF46017.1.
AY061491 mRNA. Translation: AAL29039.1.
PIRiA54279.
RefSeqiNP_511045.1. NM_078490.4.
UniGeneiDm.2625.

Genome annotation databases

EnsemblMetazoaiFBtr0070748; FBpp0070716; FBgn0003449.
GeneIDi31442.
KEGGidme:Dmel_CG4528.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89775 mRNA. Translation: AAA28441.1.
L29521 Genomic DNA. Translation: AAA28903.1.
AE014298 Genomic DNA. Translation: AAF46017.1.
AY061491 mRNA. Translation: AAL29039.1.
PIRiA54279.
RefSeqiNP_511045.1. NM_078490.4.
UniGeneiDm.2625.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AYMNMR-A134-216[»]
2B0GNMR-A134-216[»]
2K3KNMR-A1-98[»]
ProteinModelPortaliP43332.
SMRiP43332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi57952. 9 interactors.
DIPiDIP-23676N.
IntActiP43332. 2 interactors.
MINTiMINT-1634151.
STRINGi7227.FBpp0070716.

Proteomic databases

PaxDbiP43332.
PRIDEiP43332.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0070748; FBpp0070716; FBgn0003449.
GeneIDi31442.
KEGGidme:Dmel_CG4528.

Organism-specific databases

CTDi31442.
FlyBaseiFBgn0003449. snf.

Phylogenomic databases

eggNOGiKOG4206. Eukaryota.
ENOG410XPZI. LUCA.
GeneTreeiENSGT00390000007046.
InParanoidiP43332.
OMAiESANEAR.
OrthoDBiEOG091G0NFU.
PhylomeDBiP43332.

Enzyme and pathway databases

ReactomeiR-DME-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSisnf. fly.
EvolutionaryTraceiP43332.
GenomeRNAii31442.
PROiP43332.

Gene expression databases

BgeeiFBgn0003449.
GenevisibleiP43332. DM.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR024888. RRM_U1_A/B''.
[Graphical view]
PANTHERiPTHR10501. PTHR10501. 1 hit.
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSNRPA_DROME
AccessioniPrimary (citable) accession number: P43332
Secondary accession number(s): Q9W4D7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.