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Protein

ATP-dependent RNA helicase HrpA

Gene

hrpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Not yet known.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi100 – 1078ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • helicase activity Source: EcoliWiki
  • poly(A) RNA binding Source: GO_Central

GO - Biological processi

  • mRNA processing Source: EcoCyc
  • RNA modification Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6732-MONOMER.
ECOL316407:JW5905-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase HrpA (EC:3.6.4.13)
Gene namesi
Name:hrpA
Ordered Locus Names:b1413, JW5905
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12936. hrpA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13001300ATP-dependent RNA helicase HrpAPRO_0000055178Add
BLAST

Proteomic databases

PaxDbiP43329.
PRIDEiP43329.

Interactioni

Protein-protein interaction databases

BioGridi4261507. 104 interactions.
DIPiDIP-9937N.
IntActiP43329. 26 interactions.
MINTiMINT-1229564.
STRINGi511145.b1413.

Structurei

3D structure databases

ProteinModelPortaliP43329.
SMRiP43329. Positions 77-696.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini87 – 250164Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini274 – 444171Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi197 – 2004DEAH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105CQ7. Bacteria.
COG1643. LUCA.
HOGENOMiHOG000247272.
InParanoidiP43329.
KOiK03578.
OMAiYIKLMTD.
OrthoDBiEOG6PP9H5.
PhylomeDBiP43329.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR024590. HrpA_C.
IPR027417. P-loop_NTPase.
IPR010222. RNA_helicase_HrpA.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF11898. DUF3418. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01967. DEAH_box_HrpA. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43329-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEQQKLTFT ALQQRLDSLM LRDRLRFSRR LHGVKKVKNP DAQQAIFQEM
60 70 80 90 100
AKEIDQAAGK VLLREAARPE ITYPDNLPVS QKKQDILEAI RDHQVVIVAG
110 120 130 140 150
ETGSGKTTQL PKICMELGRG IKGLIGHTQP RRLAARTVAN RIAEELKTEP
160 170 180 190 200
GGCIGYKVRF SDHVSDNTMV KLMTDGILLA EIQQDRLLMQ YDTIIIDEAH
210 220 230 240 250
ERSLNIDFLL GYLKELLPRR PDLKIIITSA TIDPERFSRH FNNAPIIEVS
260 270 280 290 300
GRTYPVEVRY RPIVEEADDT ERDQLQAIFD AVDELSQESH GDILIFMSGE
310 320 330 340 350
REIRDTADAL NKLNLRHTEI LPLYARLSNS EQNRVFQSHS GRRIVLATNV
360 370 380 390 400
AETSLTVPGI KYVIDPGTAR ISRYSYRTKV QRLPIEPISQ ASANQRKGRC
410 420 430 440 450
GRVSEGICIR LYSEDDFLSR PEFTDPEILR TNLASVILQM TALGLGDIAA
460 470 480 490 500
FPFVEAPDKR NIQDGVRLLE ELGAITTDEQ ASAYKLTPLG RQLSQLPVDP
510 520 530 540 550
RLARMVLEAQ KHGCVREAMI ITSALSIQDP RERPMDKQQA SDEKHRRFHD
560 570 580 590 600
KESDFLAFVN LWNYLGEQQK ALSSNAFRRL CRTDYLNYLR VREWQDIYTQ
610 620 630 640 650
LRQVVKELGI PVNSEPAEYR EIHIALLTGL LSHIGMKDAD KQEYTGARNA
660 670 680 690 700
RFSIFPGSGL FKKPPKWVMV AELVETSRLW GRIAARIDPE WVEPVAQHLI
710 720 730 740 750
KRTYSEPHWE RAQGAVMATE KVTVYGLPIV AARKVNYSQI DPALCRELFI
760 770 780 790 800
RHALVEGDWQ TRHAFFRENL KLRAEVEELE HKSRRRDILV DDETLFEFYD
810 820 830 840 850
QRISHDVISA RHFDSWWKKV SRETPDLLNF EKSMLIKEGA EKISKLDYPN
860 870 880 890 900
FWHQGNLKLR LSYQFEPGAD ADGVTVHIPL PLLNQVEESG FEWQIPGLRR
910 920 930 940 950
ELVIALIKSL PKPVRRNFVP APNYAEAFLG RVKPLELPLL DSLERELRRM
960 970 980 990 1000
TGVTVDREDW HWDQVPDHLK ITFRVVDDKN KKLKEGRSLQ DLKDALKGKV
1010 1020 1030 1040 1050
QETLSAVADD GIEQSGLHIW SFGQLPESYE QKRGNYKVKA WPALVDERDS
1060 1070 1080 1090 1100
VAIKLFDNPL EQKQAMWNGL RRLLLLNIPS PIKYLHEKLP NKAKLGLYFN
1110 1120 1130 1140 1150
PYGKVLELID DCISCGVDKL IDANGGPVWT EEGFAALHEK VRAELNDTVV
1160 1170 1180 1190 1200
DIAKQVEQIL TAVFNINKRL KGRVDMTMAL GLSDIKAQMG GLVYRGFVTG
1210 1220 1230 1240 1250
NGFKRLGDTL RYLQAIEKRL EKLAVDPHRD RAQMLKVENV QQAWQQWINK
1260 1270 1280 1290 1300
LPPARREDED VKEIRWMIEE LRVSYFAQQL GTPYPISDKR ILQAMEQISG
Length:1,300
Mass (Da):149,028
Last modified:April 27, 2001 - v3
Checksum:iA26601266D771638
GO

Sequence cautioni

The sequence BAA07685.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAA15029.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 1521G → V in BAA07685 (PubMed:7899078).Curated
Sequence conflicti202 – 2021R → H in BAA07685 (PubMed:7899078).Curated
Sequence conflicti301 – 3011Missing in BAA07685 (PubMed:7899078).Curated
Sequence conflicti304 – 3041R → P in BAA07685 (PubMed:7899078).Curated
Sequence conflicti681 – 69414GRIAA…EWVEP → AHCCVSTRNGGA (PubMed:7899078).CuratedAdd
BLAST
Sequence conflicti705 – 7051S → T in BAA07685 (PubMed:7899078).Curated
Sequence conflicti712 – 7132AQ → E in BAA07685 (PubMed:7899078).Curated
Sequence conflicti869 – 8691A → P in BAA07685 (PubMed:7899078).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42105 Genomic DNA. Translation: BAA07685.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74495.2.
AP009048 Genomic DNA. Translation: BAA15029.1. Different initiation.
PIRiH64892.
RefSeqiNP_415931.4. NC_000913.3.
WP_000139543.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74495; AAC74495; b1413.
BAA15029; BAA15029; BAA15029.
GeneIDi948444.
KEGGiecj:JW5905.
eco:b1413.
PATRICi32118112. VBIEscCol129921_1476.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42105 Genomic DNA. Translation: BAA07685.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74495.2.
AP009048 Genomic DNA. Translation: BAA15029.1. Different initiation.
PIRiH64892.
RefSeqiNP_415931.4. NC_000913.3.
WP_000139543.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP43329.
SMRiP43329. Positions 77-696.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261507. 104 interactions.
DIPiDIP-9937N.
IntActiP43329. 26 interactions.
MINTiMINT-1229564.
STRINGi511145.b1413.

Proteomic databases

PaxDbiP43329.
PRIDEiP43329.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74495; AAC74495; b1413.
BAA15029; BAA15029; BAA15029.
GeneIDi948444.
KEGGiecj:JW5905.
eco:b1413.
PATRICi32118112. VBIEscCol129921_1476.

Organism-specific databases

EchoBASEiEB2772.
EcoGeneiEG12936. hrpA.

Phylogenomic databases

eggNOGiENOG4105CQ7. Bacteria.
COG1643. LUCA.
HOGENOMiHOG000247272.
InParanoidiP43329.
KOiK03578.
OMAiYIKLMTD.
OrthoDBiEOG6PP9H5.
PhylomeDBiP43329.

Enzyme and pathway databases

BioCyciEcoCyc:G6732-MONOMER.
ECOL316407:JW5905-MONOMER.

Miscellaneous databases

PROiP43329.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR024590. HrpA_C.
IPR027417. P-loop_NTPase.
IPR010222. RNA_helicase_HrpA.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF11898. DUF3418. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01967. DEAH_box_HrpA. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the hrpA gene in the terC region of Escherichia coli that is highly similar to the DEAH family RNA helicase genes of Saccharomyces cerevisiae."
    Moriya H., Kasai H., Isono K.
    Nucleic Acids Res. 23:595-598(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiHRPA_ECOLI
AccessioniPrimary (citable) accession number: P43329
Secondary accession number(s): P76861, P76863, P77479
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 27, 2001
Last modified: April 13, 2016
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.