P43317 (GUN5_HYPJE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase-5
EC=3.2.1.4

Alternative name(s):
Cellulase V
Endo-1,4-beta-glucanase V
Short name=EG V
Endoglucanase V

Gene names

Name:

egl5

Organism

Hypocrea jecorina (Trichoderma reesei)

Taxonomic identifier

51453 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Hypocreomycetidae › Hypocreales › Hypocreaceae › Hypocrea › mitosporic Hypocrea › Trichoderma

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 45 (cellulase K) family. Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC cellulose binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 242	225	Endoglucanase-5		PRO_0000008022
Regions
	Domain	205 – 241	37	CBM1
	Region	18 – 182	165	Catalytic
	Compositional bias	183 – 205	23	Pro/Ser-rich (linker)
Sites
	Active site	27	1	Nucleophile By similarity
	Active site	134	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	182	1	N-linked (GlcNAc...) Potential
	Disulfide bond	213 ↔ 230		By similarity
	Disulfide bond	224 ↔ 240		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43317 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: CC033FC51326C71D	FASTA	242	24,411
	10 20 30 40 50 60 MKATLVLGSL IVGAVSAYKA TTTRYYDGQE GACGCGSSSG AFPWQLGIGN GVYTAAGSQA 70 80 90 100 110 120 LFDTAGASWC GAGCGKCYQL TSTGQAPCSS CGTGGAAGQS IIVMVTNLCP NNGNAQWCPV 130 140 150 160 170 180 VGGTNQYGYS YHFDIMAQNE IFGDNVVVDF EPIACPGQAA SDWGTCLCVG QQETDPTPVL 190 200 210 220 230 240 GNDTGSTPPG SSPPATSSSP PSGGGQQTLY GQCGGAGWTG PTTCQAPGTC KVQNQWYSQC LP

References

[1]

"A novel, small endoglucanase gene, egl5, from Trichoderma reesei isolated by expression in yeast." Saloheimo A., Henrissat B., Hoffren A.-M., Teleman O., Penttilae M. Mol. Microbiol. 13:219-228(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26921 / CBS 392.92 / QM9414.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z33381 Genomic DNA. Translation: CAA83846.1.
PIR	S60143.
3D structure databases
ProteinModelPortal	P43317.
SMR	P43317. Positions 207-241.
ModBase	Search...
Protein-protein interaction databases
STRING	51453.JGI49976.
Protein family/group databases
CAZy	CBM1. Carbohydrate-Binding Module Family 1. GH45. Glycoside Hydrolase Family 45.
mycoCLAP	EGL45E_TRIRE.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG321569.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16505.
Family and domain databases
Gene3D	2.40.40.10. 1 hit.
InterPro	IPR014733. Barwin-like_endoglucanase. IPR009009. Barwin-related_endoglucanase. IPR000254. Cellulose-bd_dom_fun. IPR007112. Expansin/allergen_DPBB_dom. IPR000334. Glyco_hydro_45. [Graphical view]
Pfam	PF00734. CBM_1. 1 hit. PF02015. Glyco_hydro_45. 1 hit. [Graphical view]
ProDom	PD001821. CBD_fun. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00236. fCBD. 1 hit. [Graphical view]
SUPFAM	SSF50685. Barwin_like. 1 hit. SSF57180. CBD_fun. 1 hit.
PROSITE	PS00562. CBM1_1. 1 hit. PS51164. CBM1_2. 1 hit. PS50842. EXPANSIN_EG45. 1 hit. PS01140. GLYCOSYL_HYDROL_F45. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN5_HYPJE

Accession

Primary (citable) accession number: P43317

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program