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P43317 (GUN5_HYPJE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase-5

EC=3.2.1.4
Alternative name(s):
Cellulase V
Endo-1,4-beta-glucanase V
Short name=EG V
Endoglucanase V
Gene names
Name:egl5
OrganismHypocrea jecorina (Trichoderma reesei)
Taxonomic identifier51453 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

Protein attributes

Sequence length242 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sequence similarities

Belongs to the glycosyl hydrolase 45 (cellulase K) family.

Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

cellulose binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 242225Endoglucanase-5
PRO_0000008022

Regions

Domain205 – 24137CBM1
Region18 – 182165Catalytic
Compositional bias183 – 20523Pro/Ser-rich (linker)

Sites

Active site271Nucleophile By similarity
Active site1341Proton donor By similarity

Amino acid modifications

Glycosylation1821N-linked (GlcNAc...) Potential
Disulfide bond213 ↔ 230 By similarity
Disulfide bond224 ↔ 240 By similarity

Sequences

Sequence LengthMass (Da)Tools
P43317 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: CC033FC51326C71D

FASTA24224,411
        10         20         30         40         50         60 
MKATLVLGSL IVGAVSAYKA TTTRYYDGQE GACGCGSSSG AFPWQLGIGN GVYTAAGSQA 

        70         80         90        100        110        120 
LFDTAGASWC GAGCGKCYQL TSTGQAPCSS CGTGGAAGQS IIVMVTNLCP NNGNAQWCPV 

       130        140        150        160        170        180 
VGGTNQYGYS YHFDIMAQNE IFGDNVVVDF EPIACPGQAA SDWGTCLCVG QQETDPTPVL 

       190        200        210        220        230        240 
GNDTGSTPPG SSPPATSSSP PSGGGQQTLY GQCGGAGWTG PTTCQAPGTC KVQNQWYSQC 


LP 

« Hide

References

[1]"A novel, small endoglucanase gene, egl5, from Trichoderma reesei isolated by expression in yeast."
Saloheimo A., Henrissat B., Hoffren A.-M., Teleman O., Penttilae M.
Mol. Microbiol. 13:219-228(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 26921 / CBS 392.92 / QM9414.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z33381 Genomic DNA. Translation: CAA83846.1.
PIRS60143.

3D structure databases

ProteinModelPortalP43317.
SMRP43317. Positions 207-241.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING51453.JGI49976.

Protein family/group databases

CAZyCBM1. Carbohydrate-Binding Module Family 1.
GH45. Glycoside Hydrolase Family 45.
mycoCLAPEGL45E_TRIRE.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGNOG321569.
OMACPGQATS.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16505.

Family and domain databases

Gene3D2.40.40.10. 1 hit.
InterProIPR014733. Barwin-like_endoglucanase.
IPR000254. Cellulose-bd_dom_fun.
IPR007112. Expansin/allergen_DPBB_dom.
IPR000334. Glyco_hydro_45.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamPF00734. CBM_1. 1 hit.
PF02015. Glyco_hydro_45. 1 hit.
[Graphical view]
ProDomPD001821. CBD_fun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00236. fCBD. 1 hit.
[Graphical view]
SUPFAMSSF50685. SSF50685. 1 hit.
SSF57180. SSF57180. 1 hit.
PROSITEPS00562. CBM1_1. 1 hit.
PS51164. CBM1_2. 1 hit.
PS50842. EXPANSIN_EG45. 1 hit.
PS01140. GLYCOSYL_HYDROL_F45. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN5_HYPJE
AccessionPrimary (citable) accession number: P43317
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 16, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries