P43316 (GUN5_HUMIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Endoglucanase-5 EC=3.2.1.4 Alternative name(s): Cellulase V Endo-1,4-beta-glucanase V Short name=EG V Endoglucanase V
Organism	Humicola insolens (Soft-rot fungus)
Taxonomic identifier	34413 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › mitosporic Ascomycota › Humicola

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 45 (cellulase K) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 213

213

Endoglucanase-5

PRO_0000184074

Sites

Active site

10

1

Nucleophile

Active site

121

1

Proton donor

Secondary structure

1

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213

Helix Strand Turn

Details...

Beta strand

2 – 8

7

Helix

15 – 17

3

Beta strand

21 – 26

6

Beta strand

62 – 65

4

Beta strand

68 – 76

9

Helix

82 – 85

4

Beta strand

89 – 94

6

Helix

97 – 99

3

Beta strand

103 – 110

8

Beta strand

119 – 123

5

Turn

130 – 132

3

Helix

135 – 139

5

Beta strand

143 – 145

3

Turn

146 – 148

3

Helix

153 – 158

6

Helix

161 – 163

3

Helix

164 – 171

8

Turn

172 – 176

5

Beta strand

181 – 187

7

Helix

191 – 197

7

Helix

204 – 206

3

Sequences

	Sequence		Length	Mass (Da)
	P43316 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 24334301BA3BC804	FASTA	213	22,864
	10 20 30 40 50 60 ADGRSTRYWD CCKPSCGWAK KAPVNQPVFS CNANFQRITD FDAKSGCEPG GVAYSCADQT 70 80 90 100 110 120 PWAVNDDFAL GFAATSIAGS NEAGWCCACY ELTFTSGPVA GKKMVVQSTS TGGDLGSNHF 130 140 150 160 170 180 DLNIPGGGVG IFDGCTPQFG GLPGQRYGGI SSRNECDRFP DALKPGCYWR FDWFKNADNP 190 200 210 SFSFRQVQCP AELVARTGCR RNDDGNFPAV QIP

References

[1]	"A cellulase preparation comprising an endoglucanase enzyme." Rasmussen G., Mikkelsen J.-M., Schuelein M., Patkar S.A., Hagen F., Hjort C.M., Hastrup S. Patent number WO9117243, 14-NOV-1991 Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Structure and function of endoglucanase V." Davies G.J., Dodson G.G., Hubbard R.E., Tolley S.P., Dauter Z., Wilson K.S., Hjort C., Mikkelsen J.M., Rasmussen G., Schuelein M. Nature 365:362-364(1993) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
[3]	"Structures of oligosaccharide-bound forms of the endoglucanase V from Humicola insolens at 1.9-A resolution." Davies G.J., Tolley S.P., Henrissat B., Hjort C., Schuelein M. Biochemistry 34:16210-16220(1995) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[4]	"Structure determination and refinement of the Humicola insolens endoglucanase V at 1.5-A resolution." Davies G.J., Dodson G.G., Moore M.H., Tolley S.P., Dauter Z., Wilson K.S., Rasmussen G., Schuelein M. Acta Crystallogr. D 52:7-17(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HD5	X-ray	1.70	A	1-213	[»]
2ENG	X-ray	1.50	A	1-210	[»]
3ENG	X-ray	1.90	A	1-213	[»]
4ENG	X-ray	1.90	A	1-210	[»]

ProteinModelPortal

P43316.

SMR

P43316. Positions 1-213.

ModBase

Search...

Protein family/group databases

CAZy

CBM1. Carbohydrate-Binding Module Family 1.
GH45. Glycoside Hydrolase Family 45.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.40.40.10. 1 hit.

InterPro

IPR014733. Barwin-like_endoglucanase.
IPR009009. Barwin-related_endoglucanase.
IPR000334. Glyco_hydro_45.
[Graphical view]

Pfam

PF02015. Glyco_hydro_45. 1 hit.
[Graphical view]

SUPFAM

SSF50685. Barwin_like. 1 hit.

PROSITE

PS01140. GLYCOSYL_HYDROL_F45. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P43316.

ChEMBL

CHEMBL1795107.

EvolutionaryTrace

P43316.

Entry information

Entry name

GUN5_HUMIN

Accession

Primary (citable) accession number: P43316

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program