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P43316

- GUN5_HUMIN

UniProt

P43316 - GUN5_HUMIN

Protein

Endoglucanase-5

Gene
N/A
Organism
Humicola insolens (Soft-rot fungus)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei10 – 101Nucleophile
    Active sitei121 – 1211Proton donor

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Protein family/group databases

    CAZyiCBM1. Carbohydrate-Binding Module Family 1.
    GH45. Glycoside Hydrolase Family 45.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase-5 (EC:3.2.1.4)
    Alternative name(s):
    Cellulase V
    Endo-1,4-beta-glucanase V
    Short name:
    EG V
    Endoglucanase V
    OrganismiHumicola insolens (Soft-rot fungus)
    Taxonomic identifieri34413 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesChaetomiaceaeHumicola

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 213213Endoglucanase-5PRO_0000184074Add
    BLAST

    Interactioni

    Structurei

    Secondary structure

    1
    213
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 87
    Helixi15 – 173
    Beta strandi21 – 266
    Beta strandi62 – 654
    Beta strandi68 – 769
    Helixi82 – 854
    Beta strandi89 – 946
    Helixi97 – 993
    Beta strandi103 – 1108
    Beta strandi119 – 1235
    Turni130 – 1323
    Helixi135 – 1395
    Beta strandi143 – 1453
    Turni146 – 1483
    Helixi153 – 1586
    Helixi161 – 1633
    Helixi164 – 1718
    Turni172 – 1765
    Beta strandi181 – 1877
    Helixi191 – 1977
    Helixi204 – 2063

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HD5X-ray1.66A5-213[»]
    2ENGX-ray1.50A1-210[»]
    3ENGX-ray1.90A1-213[»]
    4ENGX-ray1.90A1-210[»]
    ProteinModelPortaliP43316.
    SMRiP43316. Positions 1-213.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP43316.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di2.40.40.10. 1 hit.
    InterProiIPR014733. Barwin-like_endoglucanase.
    IPR000334. Glyco_hydro_45.
    IPR009009. RlpA-like_DPBB.
    [Graphical view]
    PfamiPF02015. Glyco_hydro_45. 1 hit.
    [Graphical view]
    SUPFAMiSSF50685. SSF50685. 1 hit.
    PROSITEiPS01140. GLYCOSYL_HYDROL_F45. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43316-1 [UniParc]FASTAAdd to Basket

    « Hide

    ADGRSTRYWD CCKPSCGWAK KAPVNQPVFS CNANFQRITD FDAKSGCEPG    50
    GVAYSCADQT PWAVNDDFAL GFAATSIAGS NEAGWCCACY ELTFTSGPVA 100
    GKKMVVQSTS TGGDLGSNHF DLNIPGGGVG IFDGCTPQFG GLPGQRYGGI 150
    SSRNECDRFP DALKPGCYWR FDWFKNADNP SFSFRQVQCP AELVARTGCR 200
    RNDDGNFPAV QIP 213
    Length:213
    Mass (Da):22,864
    Last modified:November 1, 1995 - v1
    Checksum:i24334301BA3BC804
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HD5 X-ray 1.66 A 5-213 [» ]
    2ENG X-ray 1.50 A 1-210 [» ]
    3ENG X-ray 1.90 A 1-213 [» ]
    4ENG X-ray 1.90 A 1-210 [» ]
    ProteinModelPortali P43316.
    SMRi P43316. Positions 1-213.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P43316.
    ChEMBLi CHEMBL1795107.

    Protein family/group databases

    CAZyi CBM1. Carbohydrate-Binding Module Family 1.
    GH45. Glycoside Hydrolase Family 45.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P43316.

    Family and domain databases

    Gene3Di 2.40.40.10. 1 hit.
    InterProi IPR014733. Barwin-like_endoglucanase.
    IPR000334. Glyco_hydro_45.
    IPR009009. RlpA-like_DPBB.
    [Graphical view ]
    Pfami PF02015. Glyco_hydro_45. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50685. SSF50685. 1 hit.
    PROSITEi PS01140. GLYCOSYL_HYDROL_F45. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE.
    2. Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
    3. "Structures of oligosaccharide-bound forms of the endoglucanase V from Humicola insolens at 1.9-A resolution."
      Davies G.J., Tolley S.P., Henrissat B., Hjort C., Schuelein M.
      Biochemistry 34:16210-16220(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
    4. "Structure determination and refinement of the Humicola insolens endoglucanase V at 1.5-A resolution."
      Davies G.J., Dodson G.G., Moore M.H., Tolley S.P., Dauter Z., Wilson K.S., Rasmussen G., Schuelein M.
      Acta Crystallogr. D 52:7-17(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).

    Entry informationi

    Entry nameiGUN5_HUMIN
    AccessioniPrimary (citable) accession number: P43316
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3