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P43316 (GUN5_HUMIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase-5

EC=3.2.1.4
Alternative name(s):
Cellulase V
Endo-1,4-beta-glucanase V
Short name=EG V
Endoglucanase V
OrganismHumicola insolens (Soft-rot fungus)
Taxonomic identifier34413 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesChaetomiaceaeHumicola

Protein attributes

Sequence length213 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sequence similarities

Belongs to the glycosyl hydrolase 45 (cellulase K) family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   Molecular functionGlycosidase
Hydrolase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 213213Endoglucanase-5
PRO_0000184074

Sites

Active site101Nucleophile
Active site1211Proton donor

Secondary structure

........................................ 213
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P43316 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 24334301BA3BC804

FASTA21322,864
        10         20         30         40         50         60 
ADGRSTRYWD CCKPSCGWAK KAPVNQPVFS CNANFQRITD FDAKSGCEPG GVAYSCADQT 

        70         80         90        100        110        120 
PWAVNDDFAL GFAATSIAGS NEAGWCCACY ELTFTSGPVA GKKMVVQSTS TGGDLGSNHF 

       130        140        150        160        170        180 
DLNIPGGGVG IFDGCTPQFG GLPGQRYGGI SSRNECDRFP DALKPGCYWR FDWFKNADNP 

       190        200        210 
SFSFRQVQCP AELVARTGCR RNDDGNFPAV QIP 

« Hide

References

[1]"A cellulase preparation comprising an endoglucanase enzyme."
Rasmussen G., Mikkelsen J.-M., Schuelein M., Patkar S.A., Hagen F., Hjort C.M., Hastrup S.
Patent number WO9117243, 14-NOV-1991
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Structure and function of endoglucanase V."
Davies G.J., Dodson G.G., Hubbard R.E., Tolley S.P., Dauter Z., Wilson K.S., Hjort C., Mikkelsen J.M., Rasmussen G., Schuelein M.
Nature 365:362-364(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
[3]"Structures of oligosaccharide-bound forms of the endoglucanase V from Humicola insolens at 1.9-A resolution."
Davies G.J., Tolley S.P., Henrissat B., Hjort C., Schuelein M.
Biochemistry 34:16210-16220(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[4]"Structure determination and refinement of the Humicola insolens endoglucanase V at 1.5-A resolution."
Davies G.J., Dodson G.G., Moore M.H., Tolley S.P., Dauter Z., Wilson K.S., Rasmussen G., Schuelein M.
Acta Crystallogr. D 52:7-17(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1HD5X-ray1.70A1-213[»]
2ENGX-ray1.50A1-210[»]
3ENGX-ray1.90A1-213[»]
4ENGX-ray1.90A1-210[»]
ProteinModelPortalP43316.
SMRP43316. Positions 1-213.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP43316.
ChEMBLCHEMBL1795107.

Protein family/group databases

CAZyCBM1. Carbohydrate-Binding Module Family 1.
GH45. Glycoside Hydrolase Family 45.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.40.40.10. 1 hit.
InterProIPR014733. Barwin-like_endoglucanase.
IPR000334. Glyco_hydro_45.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamPF02015. Glyco_hydro_45. 1 hit.
[Graphical view]
SUPFAMSSF50685. SSF50685. 1 hit.
PROSITEPS01140. GLYCOSYL_HYDROL_F45. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP43316.

Entry information

Entry nameGUN5_HUMIN
AccessionPrimary (citable) accession number: P43316
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries