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P43316

- GUN5_HUMIN

UniProt

P43316 - GUN5_HUMIN

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Protein
Endoglucanase-5
Gene
N/A
Organism
Humicola insolens (Soft-rot fungus)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei10 – 101Nucleophile
Active sitei121 – 1211Proton donor

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM1. Carbohydrate-Binding Module Family 1.
GH45. Glycoside Hydrolase Family 45.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase-5 (EC:3.2.1.4)
Alternative name(s):
Cellulase V
Endo-1,4-beta-glucanase V
Short name:
EG V
Endoglucanase V
OrganismiHumicola insolens (Soft-rot fungus)
Taxonomic identifieri34413 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesChaetomiaceaeHumicola

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213Endoglucanase-5
PRO_0000184074Add
BLAST

Interactioni

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87
Helixi15 – 173
Beta strandi21 – 266
Beta strandi62 – 654
Beta strandi68 – 769
Helixi82 – 854
Beta strandi89 – 946
Helixi97 – 993
Beta strandi103 – 1108
Beta strandi119 – 1235
Turni130 – 1323
Helixi135 – 1395
Beta strandi143 – 1453
Turni146 – 1483
Helixi153 – 1586
Helixi161 – 1633
Helixi164 – 1718
Turni172 – 1765
Beta strandi181 – 1877
Helixi191 – 1977
Helixi204 – 2063

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HD5X-ray1.66A5-213[»]
2ENGX-ray1.50A1-210[»]
3ENGX-ray1.90A1-213[»]
4ENGX-ray1.90A1-210[»]
ProteinModelPortaliP43316.
SMRiP43316. Positions 1-213.

Miscellaneous databases

EvolutionaryTraceiP43316.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.40.40.10. 1 hit.
InterProiIPR014733. Barwin-like_endoglucanase.
IPR000334. Glyco_hydro_45.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamiPF02015. Glyco_hydro_45. 1 hit.
[Graphical view]
SUPFAMiSSF50685. SSF50685. 1 hit.
PROSITEiPS01140. GLYCOSYL_HYDROL_F45. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43316-1 [UniParc]FASTAAdd to Basket

« Hide

ADGRSTRYWD CCKPSCGWAK KAPVNQPVFS CNANFQRITD FDAKSGCEPG    50
GVAYSCADQT PWAVNDDFAL GFAATSIAGS NEAGWCCACY ELTFTSGPVA 100
GKKMVVQSTS TGGDLGSNHF DLNIPGGGVG IFDGCTPQFG GLPGQRYGGI 150
SSRNECDRFP DALKPGCYWR FDWFKNADNP SFSFRQVQCP AELVARTGCR 200
RNDDGNFPAV QIP 213
Length:213
Mass (Da):22,864
Last modified:November 1, 1995 - v1
Checksum:i24334301BA3BC804
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HD5 X-ray 1.66 A 5-213 [» ]
2ENG X-ray 1.50 A 1-210 [» ]
3ENG X-ray 1.90 A 1-213 [» ]
4ENG X-ray 1.90 A 1-210 [» ]
ProteinModelPortali P43316.
SMRi P43316. Positions 1-213.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P43316.
ChEMBLi CHEMBL1795107.

Protein family/group databases

CAZyi CBM1. Carbohydrate-Binding Module Family 1.
GH45. Glycoside Hydrolase Family 45.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P43316.

Family and domain databases

Gene3Di 2.40.40.10. 1 hit.
InterProi IPR014733. Barwin-like_endoglucanase.
IPR000334. Glyco_hydro_45.
IPR009009. RlpA-like_DPBB.
[Graphical view ]
Pfami PF02015. Glyco_hydro_45. 1 hit.
[Graphical view ]
SUPFAMi SSF50685. SSF50685. 1 hit.
PROSITEi PS01140. GLYCOSYL_HYDROL_F45. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
  3. "Structures of oligosaccharide-bound forms of the endoglucanase V from Humicola insolens at 1.9-A resolution."
    Davies G.J., Tolley S.P., Henrissat B., Hjort C., Schuelein M.
    Biochemistry 34:16210-16220(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
  4. "Structure determination and refinement of the Humicola insolens endoglucanase V at 1.5-A resolution."
    Davies G.J., Dodson G.G., Moore M.H., Tolley S.P., Dauter Z., Wilson K.S., Rasmussen G., Schuelein M.
    Acta Crystallogr. D 52:7-17(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).

Entry informationi

Entry nameiGUN5_HUMIN
AccessioniPrimary (citable) accession number: P43316
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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