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P43315 (BFR_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bacterioferritin

Short name=BFR
EC=1.16.3.1
Alternative name(s):
Major membrane protein II
Short name=MMP-II
Gene names
Name:bfr
Synonyms:bfrA
Ordered Locus Names:ML2038
OrganismMycobacterium leprae (strain TN) [Complete proteome] [HAMAP]
Taxonomic identifier272631 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length159 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Iron-storage protein, whose ferroxidase center binds Fe2+ ions, oxidizes them by dioxygen to Fe3+, and participates in the subsequent Fe3+ oxide mineral core formation within the central cavity of the protein complex By similarity. Probably plays a crucial role in the intracellular existence of this organism by functioning as a temporary depository for iron in iron deprivation.

Catalytic activity

4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.

Cofactor

Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.

Binds 2 iron ions per subunit. The catalytic dinuclear iron-binding site within each subunit is known as the ferroxidase center By similarity.

Subunit structure

Homooligomer of 24 subunits, arranged as 12 dimers, that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be deposited By similarity.

Subcellular location

Cytoplasmcytosol Probable. Membrane; Peripheral membrane protein Probable. Note: Soluble or peripheral membrane protein Probable.

Sequence similarities

Belongs to the bacterioferritin family.

Contains 1 ferritin-like diiron domain.

Ontologies

Keywords
   Biological processIron storage
   Cellular componentCytoplasm
Membrane
   LigandHeme
Iron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processcellular iron ion homeostasis

Inferred from electronic annotation. Source: UniProtKB-KW

iron ion transport

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionferric iron binding

Inferred from electronic annotation. Source: InterPro

ferroxidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 159159Bacterioferritin
PRO_0000192599

Regions

Domain1 – 145145Ferritin-like diiron

Sites

Metal binding181Iron 1 By similarity
Metal binding511Iron 1 By similarity
Metal binding511Iron 2 By similarity
Metal binding521Iron (heme axial ligand); shared with dimeric partner By similarity
Metal binding541Iron 1 By similarity
Metal binding941Iron 2 By similarity
Metal binding1271Iron 1 By similarity
Metal binding1271Iron 2 By similarity
Metal binding1301Iron 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P43315 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: D07C474A65E3AD64

FASTA15918,263
        10         20         30         40         50         60 
MQGDPDVLRL LNEQLTSELT AINQYFLHSK MQENWGFTEL AERTRVESFD EMRHAEAITD 

        70         80         90        100        110        120 
RILLLDGLPN YQRIGSLRVG QTLREQFEAD LAIEYEVMSR LKPGIIMCRE KQDSTSAVLL 

       130        140        150 
EKIVADEEEH IDYLETQLAL MGQLGEELYS AQCVSRPPS 

« Hide

References

« Hide 'large scale' references
[1]"Purification, characterization, gene sequence, and significance of a bacterioferritin from Mycobacterium leprae."
Pessolani M.C.V., Smith D.R., Rivoire B., McCormick J., Hefta S.A., Cole S.T., Brennan P.J.
J. Exp. Med. 180:319-327(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"Massive gene decay in the leprosy bacillus."
Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. expand/collapse author list , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G.
Nature 409:1007-1011(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TN.
[3]"The major native proteins of the leprosy bacillus."
Hunter S.W., Rivoire B., Mehra V., Bloom B.R., Brennan P.J.
J. Biol. Chem. 265:14065-14068(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY PROTEIN SEQUENCE OF 1-14.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L01095 Genomic DNA. Translation: AAA21339.1.
AL583924 Genomic DNA. Translation: CAC30993.1.
PIRA87164.
RefSeqNP_302363.1. NC_002677.1.

3D structure databases

ProteinModelPortalP43315.
SMRP43315. Positions 1-158.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272631.ML2038.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC30993; CAC30993; CAC30993.
GeneID909017.
KEGGmle:ML2038.
PATRIC18057832. VBIMycLep78757_3840.

Organism-specific databases

LepromaML2038.
CMRSearch...

Phylogenomic databases

eggNOGCOG2193.
HOGENOMHOG000262383.
KOK03594.
OMAEMKHADQ.
OrthoDBEOG6WDSKP.
ProtClustDBCLSK791451.

Family and domain databases

Gene3D1.20.1260.10. 1 hit.
InterProIPR002024. Bacterioferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PfamPF00210. Ferritin. 1 hit.
[Graphical view]
PIRSFPIRSF002560. Bacterioferritin. 1 hit.
PRINTSPR00601. BACFERRITIN.
SUPFAMSSF47240. SSF47240. 1 hit.
TIGRFAMsTIGR00754. bfr. 1 hit.
PROSITEPS00549. BACTERIOFERRITIN. 1 hit.
PS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBFR_MYCLE
AccessionPrimary (citable) accession number: P43315
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 13, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families