P43315 (BFR_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bacterioferritin
Short name=BFR
EC=1.16.3.1

Alternative name(s):
Major membrane protein II
Short name=MMP-II

Gene names

Name:	bfr
Synonyms:	bfrA
Ordered Locus Names:	ML2038

Organism

Mycobacterium leprae (strain TN) [Complete proteome] [HAMAP]

Taxonomic identifier

272631 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium leprae

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Iron-storage protein, whose ferroxidase center binds Fe2+ ions, oxidizes them by dioxygen to Fe3+, and participates in the subsequent Fe3+ oxide mineral core formation within the central cavity of the protein complex By similarity. Probably plays a crucial role in the intracellular existence of this organism by functioning as a temporary depository for iron in iron deprivation.
Catalytic activity	4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity. Binds 2 iron ions per subunit. The catalytic dinuclear iron-binding site within each subunit is known as the ferroxidase center By similarity.
Subunit structure	Homooligomer of 24 subunits, arranged as 12 dimers, that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be deposited By similarity.
Subcellular location	Cytoplasm › cytosol Probable. Membrane; Peripheral membrane protein Probable. Note: Soluble or peripheral membrane protein Probable.
Sequence similarities	Belongs to the bacterioferritin family. Contains 1 ferritin-like diiron domain.

Ontologies

Keywords
Biological process	Iron storage
Cellular component	Cytoplasm Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellular iron ion homeostasis Inferred from electronic annotation. Source: UniProtKB-KW iron ion transport Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ferric iron binding Inferred from electronic annotation. Source: InterPro ferroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 159	159	Bacterioferritin		PRO_0000192599
Regions
	Domain	1 – 145	145	Ferritin-like diiron
Sites
	Metal binding	18	1	Iron 1 By similarity
	Metal binding	51	1	Iron 1 By similarity
	Metal binding	51	1	Iron 2 By similarity
	Metal binding	52	1	Iron (heme axial ligand); shared with dimeric partner By similarity
	Metal binding	54	1	Iron 1 By similarity
	Metal binding	94	1	Iron 2 By similarity
	Metal binding	127	1	Iron 1 By similarity
	Metal binding	127	1	Iron 2 By similarity
	Metal binding	130	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43315 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D07C474A65E3AD64	FASTA	159	18,263
	10 20 30 40 50 60 MQGDPDVLRL LNEQLTSELT AINQYFLHSK MQENWGFTEL AERTRVESFD EMRHAEAITD 70 80 90 100 110 120 RILLLDGLPN YQRIGSLRVG QTLREQFEAD LAIEYEVMSR LKPGIIMCRE KQDSTSAVLL 130 140 150 EKIVADEEEH IDYLETQLAL MGQLGEELYS AQCVSRPPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification, characterization, gene sequence, and significance of a bacterioferritin from Mycobacterium leprae." Pessolani M.C.V., Smith D.R., Rivoire B., McCormick J., Hefta S.A., Cole S.T., Brennan P.J. J. Exp. Med. 180:319-327(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.
[3]	"The major native proteins of the leprosy bacillus." Hunter S.W., Rivoire B., Mehra V., Bloom B.R., Brennan P.J. J. Biol. Chem. 265:14065-14068(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE OF 1-14.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L01095 Genomic DNA. Translation: AAA21339.1. AL583924 Genomic DNA. Translation: CAC30993.1.
PIR	A87164.
RefSeq	NP_302363.1. NC_002677.1.
3D structure databases
ProteinModelPortal	P43315.
SMR	P43315. Positions 1-158.
ModBase	Search...
Protein-protein interaction databases
STRING	272631.ML2038.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC30993; CAC30993; CAC30993.
GeneID	909017.
KEGG	mle:ML2038.
PATRIC	18057832. VBIMycLep78757_3840.
Organism-specific databases
Leproma	ML2038.
CMR	Search...
Phylogenomic databases
eggNOG	COG2193.
HOGENOM	HOG000262383.
KO	K03594.
OMA	HHIDFLE.
ProtClustDB	CLSK791451.
Family and domain databases
Gene3D	1.20.1260.10. 1 hit.
InterPro	IPR002024. Bacterioferritin. IPR009040. Ferritin-like_diiron. IPR009078. Ferritin-like_SF. IPR012347. Ferritin-rel. IPR008331. Ferritin_DPS_dom. [Graphical view]
Pfam	PF00210. Ferritin. 1 hit. [Graphical view]
PIRSF	PIRSF002560. Bacterioferritin. 1 hit.
PRINTS	PR00601. BACFERRITIN.
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
TIGRFAMs	TIGR00754. bfr. 1 hit.
PROSITE	PS00549. BACTERIOFERRITIN. 1 hit. PS50905. FERRITIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BFR_MYCLE

Accession

Primary (citable) accession number: P43315

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program