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P43315

- BFR_MYCLE

UniProt

P43315 - BFR_MYCLE

Protein

Bacterioferritin

Gene

bfr

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Iron-storage protein, whose ferroxidase center binds Fe2+ ions, oxidizes them by dioxygen to Fe3+, and participates in the subsequent Fe3+ oxide mineral core formation within the central cavity of the protein complex By similarity. Probably plays a crucial role in the intracellular existence of this organism by functioning as a temporary depository for iron in iron deprivation.By similarity

    Catalytic activityi

    4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.

    Cofactori

    Binds 1 heme B (iron-protoporphyrin IX) group per dimer.By similarity
    Binds 2 iron ions per subunit. The catalytic dinuclear iron-binding site within each subunit is known as the ferroxidase center By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi18 – 181Iron 1PROSITE-ProRule annotation
    Metal bindingi51 – 511Iron 1PROSITE-ProRule annotation
    Metal bindingi51 – 511Iron 2PROSITE-ProRule annotation
    Metal bindingi52 – 521Iron (heme axial ligand); shared with dimeric partnerPROSITE-ProRule annotation
    Metal bindingi54 – 541Iron 1PROSITE-ProRule annotation
    Metal bindingi94 – 941Iron 2PROSITE-ProRule annotation
    Metal bindingi127 – 1271Iron 1PROSITE-ProRule annotation
    Metal bindingi127 – 1271Iron 2PROSITE-ProRule annotation
    Metal bindingi130 – 1301Iron 2PROSITE-ProRule annotation

    GO - Molecular functioni

    1. ferric iron binding Source: InterPro
    2. ferroxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular iron ion homeostasis Source: UniProtKB-KW
    2. iron ion transport Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Iron storage

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bacterioferritin (EC:1.16.3.1)
    Short name:
    BFR
    Alternative name(s):
    Major membrane protein II
    Short name:
    MMP-II
    Gene namesi
    Name:bfr
    Synonyms:bfrA
    Ordered Locus Names:ML2038
    OrganismiMycobacterium leprae (strain TN)
    Taxonomic identifieri272631 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000806: Chromosome

    Organism-specific databases

    LepromaiML2038.

    Subcellular locationi

    Cytoplasmcytosol Curated. Membrane Curated; Peripheral membrane protein Curated
    Note: Soluble or peripheral membrane protein.Curated

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 159159BacterioferritinPRO_0000192599Add
    BLAST

    Interactioni

    Subunit structurei

    Homooligomer of 24 subunits, arranged as 12 dimers, that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be deposited.By similarity

    Protein-protein interaction databases

    STRINGi272631.ML2038.

    Structurei

    3D structure databases

    ProteinModelPortaliP43315.
    SMRiP43315. Positions 1-158.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 145145Ferritin-like diironPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the bacterioferritin family.Curated
    Contains 1 ferritin-like diiron domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2193.
    HOGENOMiHOG000262383.
    KOiK03594.
    OMAiAGDYVSM.
    OrthoDBiEOG6WDSKP.

    Family and domain databases

    Gene3Di1.20.1260.10. 1 hit.
    InterProiIPR002024. Bacterioferritin.
    IPR009040. Ferritin-like_diiron.
    IPR009078. Ferritin-like_SF.
    IPR012347. Ferritin-rel.
    IPR008331. Ferritin_DPS_dom.
    [Graphical view]
    PfamiPF00210. Ferritin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002560. Bacterioferritin. 1 hit.
    PRINTSiPR00601. BACFERRITIN.
    SUPFAMiSSF47240. SSF47240. 1 hit.
    TIGRFAMsiTIGR00754. bfr. 1 hit.
    PROSITEiPS00549. BACTERIOFERRITIN. 1 hit.
    PS50905. FERRITIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43315-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQGDPDVLRL LNEQLTSELT AINQYFLHSK MQENWGFTEL AERTRVESFD    50
    EMRHAEAITD RILLLDGLPN YQRIGSLRVG QTLREQFEAD LAIEYEVMSR 100
    LKPGIIMCRE KQDSTSAVLL EKIVADEEEH IDYLETQLAL MGQLGEELYS 150
    AQCVSRPPS 159
    Length:159
    Mass (Da):18,263
    Last modified:November 1, 1995 - v1
    Checksum:iD07C474A65E3AD64
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01095 Genomic DNA. Translation: AAA21339.1.
    AL583924 Genomic DNA. Translation: CAC30993.1.
    PIRiA87164.
    RefSeqiNP_302363.1. NC_002677.1.
    WP_010908683.1. NC_002677.1.

    Genome annotation databases

    EnsemblBacteriaiCAC30993; CAC30993; CAC30993.
    GeneIDi909017.
    KEGGimle:ML2038.
    PATRICi18057832. VBIMycLep78757_3840.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01095 Genomic DNA. Translation: AAA21339.1 .
    AL583924 Genomic DNA. Translation: CAC30993.1 .
    PIRi A87164.
    RefSeqi NP_302363.1. NC_002677.1.
    WP_010908683.1. NC_002677.1.

    3D structure databases

    ProteinModelPortali P43315.
    SMRi P43315. Positions 1-158.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272631.ML2038.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC30993 ; CAC30993 ; CAC30993 .
    GeneIDi 909017.
    KEGGi mle:ML2038.
    PATRICi 18057832. VBIMycLep78757_3840.

    Organism-specific databases

    Lepromai ML2038.

    Phylogenomic databases

    eggNOGi COG2193.
    HOGENOMi HOG000262383.
    KOi K03594.
    OMAi AGDYVSM.
    OrthoDBi EOG6WDSKP.

    Family and domain databases

    Gene3Di 1.20.1260.10. 1 hit.
    InterProi IPR002024. Bacterioferritin.
    IPR009040. Ferritin-like_diiron.
    IPR009078. Ferritin-like_SF.
    IPR012347. Ferritin-rel.
    IPR008331. Ferritin_DPS_dom.
    [Graphical view ]
    Pfami PF00210. Ferritin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002560. Bacterioferritin. 1 hit.
    PRINTSi PR00601. BACFERRITIN.
    SUPFAMi SSF47240. SSF47240. 1 hit.
    TIGRFAMsi TIGR00754. bfr. 1 hit.
    PROSITEi PS00549. BACTERIOFERRITIN. 1 hit.
    PS50905. FERRITIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Purification, characterization, gene sequence, and significance of a bacterioferritin from Mycobacterium leprae."
      Pessolani M.C.V., Smith D.R., Rivoire B., McCormick J., Hefta S.A., Cole S.T., Brennan P.J.
      J. Exp. Med. 180:319-327(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TN.
    3. "The major native proteins of the leprosy bacillus."
      Hunter S.W., Rivoire B., Mehra V., Bloom B.R., Brennan P.J.
      J. Biol. Chem. 265:14065-14068(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY PROTEIN SEQUENCE OF 1-14.

    Entry informationi

    Entry nameiBFR_MYCLE
    AccessioniPrimary (citable) accession number: P43315
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3