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P43311

- PPO_VITVI

UniProt

P43311 - PPO_VITVI

Protein

Polyphenol oxidase, chloroplastic

Gene
N/A
Organism
Vitis vinifera (Grape)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.1 Publication

    Catalytic activityi

    2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O.1 Publication

    Cofactori

    Binds 2 copper ions per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi190 – 1901Copper A
    Metal bindingi211 – 2111Copper A
    Metal bindingi220 – 2201Copper A
    Metal bindingi342 – 3421Copper B
    Metal bindingi346 – 3461Copper B
    Metal bindingi375 – 3751Copper B

    GO - Molecular functioni

    1. catechol oxidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. pigment biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Copper, Metal-binding

    Enzyme and pathway databases

    BRENDAi1.10.3.1. 6671.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyphenol oxidase, chloroplastic (EC:1.10.3.1)
    Short name:
    PPO
    Alternative name(s):
    Catechol oxidase
    OrganismiVitis vinifera (Grape)
    Taxonomic identifieri29760 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast thylakoid lumen Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid, Thylakoid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 103103ChloroplastSequence AnalysisAdd
    BLAST
    Chaini104 – 607504Polyphenol oxidase, chloroplasticPRO_0000035920Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi114 ↔ 1291 Publication
    Disulfide bondi128 ↔ 1911 Publication
    Cross-linki194 ↔ 2112'-(S-cysteinyl)-histidine (Cys-His)By similarity

    Keywords - PTMi

    Disulfide bond, Thioether bond

    Proteomic databases

    PRIDEiP43311.

    Structurei

    Secondary structure

    1
    607
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi111 – 1133
    Beta strandi136 – 1383
    Beta strandi144 – 1463
    Beta strandi149 – 1513
    Helixi154 – 1563
    Helixi159 – 17315
    Helixi183 – 19412
    Beta strandi211 – 2133
    Helixi216 – 23419
    Helixi250 – 2523
    Helixi257 – 2604
    Beta strandi262 – 2643
    Helixi273 – 2753
    Helixi294 – 30916
    Turni310 – 3123
    Helixi316 – 3205
    Helixi336 – 3394
    Helixi342 – 3498
    Beta strandi352 – 3554
    Turni357 – 3604
    Turni362 – 3643
    Helixi365 – 3673
    Helixi371 – 38515
    Helixi399 – 4024
    Beta strandi405 – 4095
    Beta strandi415 – 4195
    Helixi420 – 4234
    Turni426 – 4305
    Beta strandi431 – 4333

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2P3XX-ray2.20A104-442[»]
    ProteinModelPortaliP43311.
    SMRiP43311. Positions 104-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP43311.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tyrosinase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiNOG254493.

    Family and domain databases

    Gene3Di1.10.1280.10. 1 hit.
    InterProiIPR013788. Hemocyanin/hexamerin.
    IPR016213. Polyphenol_oxidase.
    IPR022740. Polyphenol_oxidase_C.
    IPR022739. Polyphenol_oxidase_cen.
    IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view]
    PfamiPF12142. PPO1_DWL. 1 hit.
    PF12143. PPO1_KFDV. 1 hit.
    PF00264. Tyrosinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000290. PPO_plant. 1 hit.
    PRINTSiPR00092. TYROSINASE.
    SUPFAMiSSF48056. SSF48056. 1 hit.
    PROSITEiPS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43311-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASLPWSLTT STAIANTTNI SAFPPSPLFQ RASHVPVARN RSRRFAPSKV    50
    SCNSANGDPN SDSTSDVRET SSGKLDRRNV LLGIGGLYGA AGGLGATKPL 100
    AFGAPIQAPD ISKCGTATVP DGVTPTNCCP PVTTKIIDFQ LPSSGSPMRT 150
    RPAAHLVSKE YLAKYKKAIE LQKALPDDDP RSFKQQANVH CTYCQGAYDQ 200
    VGYTDLELQV HASWLFLPFH RYYLYFNERI LAKLIDDPTF ALPYWAWDNP 250
    DGMYMPTIYA SSPSSLYDEK RNAKHLPPTV IDLDYDGTEP TIPDDELKTD 300
    NLAIMYKQIV SGATTPKLFL GYPYRAGDAI DPGAGTLEHA PHNIVHKWTG 350
    LADKPSEDMG NFYTAGRDPI FFGHHANVDR MWNIWKTIGG KNRKDFTDTD 400
    WLDATFVFYD ENKQLVKVKV SDCVDTSKLR YQYQDIPIPW LPKNTKAKAK 450
    TTTKSSKSGV AKAAELPKTT ISSIGDFPKA LNSVIRVEVP RPKKSRSKKE 500
    KEDEEEVLLI KGIELDRENF VKFDVYINDE DYSVSRPKNS EFAGSFVNVP 550
    HKHMKEMKTK TNLRFAINEL LEDLGAEDDE SVIVTIVPRA GGDDVTIGGI 600
    EIEFVSD 607
    Length:607
    Mass (Da):67,347
    Last modified:November 1, 1995 - v1
    Checksum:iB9045598E69BC57B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z27411 mRNA. Translation: CAA81798.1.
    UniGeneiVvi.108.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z27411 mRNA. Translation: CAA81798.1 .
    UniGenei Vvi.108.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2P3X X-ray 2.20 A 104-442 [» ]
    ProteinModelPortali P43311.
    SMRi P43311. Positions 104-441.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P43311.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi NOG254493.

    Enzyme and pathway databases

    BRENDAi 1.10.3.1. 6671.

    Miscellaneous databases

    EvolutionaryTracei P43311.

    Family and domain databases

    Gene3Di 1.10.1280.10. 1 hit.
    InterProi IPR013788. Hemocyanin/hexamerin.
    IPR016213. Polyphenol_oxidase.
    IPR022740. Polyphenol_oxidase_C.
    IPR022739. Polyphenol_oxidase_cen.
    IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view ]
    Pfami PF12142. PPO1_DWL. 1 hit.
    PF12143. PPO1_KFDV. 1 hit.
    PF00264. Tyrosinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000290. PPO_plant. 1 hit.
    PRINTSi PR00092. TYROSINASE.
    SUPFAMi SSF48056. SSF48056. 1 hit.
    PROSITEi PS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterisation of grape berry polyphenol oxidase."
      Dry I.B., Robinson S.P.
      Plant Mol. Biol. 26:495-502(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Sultana.
      Tissue: Fruit.
    2. "Cloning, sequencing, purification, and crystal structure of Grenache (Vitis vinifera) polyphenol oxidase."
      Virador V.M., Reyes Grajeda J.P., Blanco-Labra A., Mendiola-Olaya E., Smith G.M., Moreno A., Whitaker J.R.
      J. Agric. Food Chem. 58:1189-1201(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 104-442 IN COMPLEX WITH COPPER IONS, FUNCTION, CATALYTIC ACTIVITY, DISULFIDE BONDS.

    Entry informationi

    Entry nameiPPO_VITVI
    AccessioniPrimary (citable) accession number: P43311
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3