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Protein

Polyphenol oxidase, chloroplastic

Gene
N/A
Organism
Vitis vinifera (Grape)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.1 Publication

Catalytic activityi

2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O.1 Publication

Cofactori

Cu2+1 PublicationNote: Binds 2 copper ions per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi190Copper A1 Publication1
Metal bindingi211Copper A1 Publication1
Metal bindingi220Copper A1 Publication1
Metal bindingi342Copper B1 Publication1
Metal bindingi346Copper B1 Publication1
Metal bindingi375Copper B1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BRENDAi1.10.3.1. 6671.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyphenol oxidase, chloroplastic (EC:1.10.3.1)
Short name:
PPO
Alternative name(s):
Catechol oxidase
OrganismiVitis vinifera (Grape)
Taxonomic identifieri29760 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 103ChloroplastSequence analysisAdd BLAST103
ChainiPRO_0000035920104 – 607Polyphenol oxidase, chloroplasticAdd BLAST504

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi114 ↔ 1291 Publication
Disulfide bondi128 ↔ 1911 Publication
Cross-linki194 ↔ 2112'-(S-cysteinyl)-histidine (Cys-His)By similarity

Keywords - PTMi

Disulfide bond, Thioether bond

Proteomic databases

PRIDEiP43311.

Interactioni

Protein-protein interaction databases

STRINGi29760.VIT_10s0116g00560.t01.

Structurei

Secondary structure

1607
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi111 – 113Combined sources3
Beta strandi136 – 138Combined sources3
Beta strandi144 – 146Combined sources3
Beta strandi149 – 151Combined sources3
Helixi154 – 156Combined sources3
Helixi159 – 173Combined sources15
Helixi183 – 194Combined sources12
Beta strandi211 – 213Combined sources3
Helixi216 – 234Combined sources19
Helixi250 – 252Combined sources3
Helixi257 – 260Combined sources4
Beta strandi262 – 264Combined sources3
Helixi273 – 275Combined sources3
Helixi294 – 309Combined sources16
Turni310 – 312Combined sources3
Helixi316 – 320Combined sources5
Helixi336 – 339Combined sources4
Helixi342 – 349Combined sources8
Beta strandi352 – 355Combined sources4
Turni357 – 360Combined sources4
Turni362 – 364Combined sources3
Helixi365 – 367Combined sources3
Helixi371 – 385Combined sources15
Helixi399 – 402Combined sources4
Beta strandi405 – 409Combined sources5
Beta strandi415 – 419Combined sources5
Helixi420 – 423Combined sources4
Turni426 – 430Combined sources5
Beta strandi431 – 433Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P3XX-ray2.20A104-442[»]
ProteinModelPortaliP43311.
SMRiP43311.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43311.

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR013788. Hemocyanin/hexamerin.
IPR016213. Polyphenol_oxidase.
IPR022740. Polyphenol_oxidase_C.
IPR022739. Polyphenol_oxidase_cen.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF12142. PPO1_DWL. 1 hit.
PF12143. PPO1_KFDV. 1 hit.
PF00264. Tyrosinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000290. PPO_plant. 1 hit.
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43311-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLPWSLTT STAIANTTNI SAFPPSPLFQ RASHVPVARN RSRRFAPSKV
60 70 80 90 100
SCNSANGDPN SDSTSDVRET SSGKLDRRNV LLGIGGLYGA AGGLGATKPL
110 120 130 140 150
AFGAPIQAPD ISKCGTATVP DGVTPTNCCP PVTTKIIDFQ LPSSGSPMRT
160 170 180 190 200
RPAAHLVSKE YLAKYKKAIE LQKALPDDDP RSFKQQANVH CTYCQGAYDQ
210 220 230 240 250
VGYTDLELQV HASWLFLPFH RYYLYFNERI LAKLIDDPTF ALPYWAWDNP
260 270 280 290 300
DGMYMPTIYA SSPSSLYDEK RNAKHLPPTV IDLDYDGTEP TIPDDELKTD
310 320 330 340 350
NLAIMYKQIV SGATTPKLFL GYPYRAGDAI DPGAGTLEHA PHNIVHKWTG
360 370 380 390 400
LADKPSEDMG NFYTAGRDPI FFGHHANVDR MWNIWKTIGG KNRKDFTDTD
410 420 430 440 450
WLDATFVFYD ENKQLVKVKV SDCVDTSKLR YQYQDIPIPW LPKNTKAKAK
460 470 480 490 500
TTTKSSKSGV AKAAELPKTT ISSIGDFPKA LNSVIRVEVP RPKKSRSKKE
510 520 530 540 550
KEDEEEVLLI KGIELDRENF VKFDVYINDE DYSVSRPKNS EFAGSFVNVP
560 570 580 590 600
HKHMKEMKTK TNLRFAINEL LEDLGAEDDE SVIVTIVPRA GGDDVTIGGI

EIEFVSD
Length:607
Mass (Da):67,347
Last modified:November 1, 1995 - v1
Checksum:iB9045598E69BC57B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z27411 mRNA. Translation: CAA81798.1.
UniGeneiVvi.108.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z27411 mRNA. Translation: CAA81798.1.
UniGeneiVvi.108.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P3XX-ray2.20A104-442[»]
ProteinModelPortaliP43311.
SMRiP43311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi29760.VIT_10s0116g00560.t01.

Proteomic databases

PRIDEiP43311.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi1.10.3.1. 6671.

Miscellaneous databases

EvolutionaryTraceiP43311.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR013788. Hemocyanin/hexamerin.
IPR016213. Polyphenol_oxidase.
IPR022740. Polyphenol_oxidase_C.
IPR022739. Polyphenol_oxidase_cen.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF12142. PPO1_DWL. 1 hit.
PF12143. PPO1_KFDV. 1 hit.
PF00264. Tyrosinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000290. PPO_plant. 1 hit.
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPO_VITVI
AccessioniPrimary (citable) accession number: P43311
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.