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P43310

- PPO_SPIOL

UniProt

P43310 - PPO_SPIOL

Protein

Polyphenol oxidase, chloroplastic

Gene
N/A
Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.

    Catalytic activityi

    2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O.

    Cofactori

    Binds 2 copper ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi193 – 1931Copper ABy similarity
    Metal bindingi214 – 2141Copper ABy similarity
    Metal bindingi223 – 2231Copper ABy similarity
    Metal bindingi354 – 3541Copper BBy similarity
    Metal bindingi358 – 3581Copper BBy similarity
    Metal bindingi388 – 3881Copper BBy similarity

    GO - Molecular functioni

    1. catechol oxidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. pigment biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyphenol oxidase, chloroplastic (EC:1.10.3.1)
    Short name:
    PPO
    Alternative name(s):
    Catechol oxidase
    OrganismiSpinacia oleracea (Spinach)
    Taxonomic identifieri3562 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast thylakoid lumen Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid, Thylakoid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 101101ChloroplastSequence AnalysisAdd
    BLAST
    Chaini102 – 639538Polyphenol oxidase, chloroplasticPRO_0000035918Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi111 ↔ 127By similarity
    Disulfide bondi126 ↔ 194By similarity
    Cross-linki197 ↔ 2142'-(S-cysteinyl)-histidine (Cys-His)By similarity

    Keywords - PTMi

    Disulfide bond, Thioether bond

    Structurei

    3D structure databases

    ProteinModelPortaliP43310.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tyrosinase family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di1.10.1280.10. 1 hit.
    InterProiIPR016213. Polyphenol_oxidase.
    IPR022740. Polyphenol_oxidase_C.
    IPR022739. Polyphenol_oxidase_cen.
    IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view]
    PfamiPF12142. PPO1_DWL. 1 hit.
    PF12143. PPO1_KFDV. 1 hit.
    PF00264. Tyrosinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000290. PPO_plant. 1 hit.
    PRINTSiPR00092. TYROSINASE.
    SUPFAMiSSF48056. SSF48056. 1 hit.
    PROSITEiPS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43310-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATLSSPTII TTTSILLNNP FLPKTPQLSA HHHRGVRSVN GKVSCQTKNN    50
    NGNDENNQFQ LIQNPNTNTP YLLDRRNILL GLGGMYAALG SEGANYYNTL 100
    AAPILPDVEK CTLSDALWDG SVGDHCCPPP FDLNITKDFE FKNYHNHVKK 150
    VRRPAHKAYE DQEWLNDYKR AIAIMKSLPM SDPRSHMQQA RVHCAYCDGS 200
    YPVLGHNDTR LEVHASWLFP SFHRWYLYFY ERILGKLINK PDFALPYWNW 250
    DHRDGMRIPE IFKEMDSPLF DPNRNTNHLD KMMNLSFVSD EEGSDVNEDD 300
    QYEENILLMR KAMVYPSVSD DPNKAELFLG SPYRAGDKME GDVSGAGILE 350
    RMPHNSVHVW TRSNTIKGNQ DMGAFWSAGR DPLFYCHHSN VDRMWSLWTD 400
    VLHGGNFPKT PEYDDYRNAY FYFYDENANP VRVYVRDSFD TERLGYKYED 450
    QELPWMSITQ QQQQQQRQQQ RQPLLGGRLK TRTFSLVKKV LTELKVMLPL 500
    PLKYSVIKTK VDRPKKSRTK EDKLEHEEVL VINFKLGKSK DFIKFDVYIN 550
    DGTDYKPEDK TKINLEYAGS FTSLTHGGGG GGGDMSHMAE EDMGKNTVLK 600
    LALNQLLEDL DATDDDSIQV TIVPKSGTDS IVITGIDIE 639
    Length:639
    Mass (Da):73,237
    Last modified:November 1, 1995 - v1
    Checksum:i56917BBF12F5162A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19270 mRNA. Translation: AAC49041.1.
    Z66559 mRNA. Translation: CAA91448.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19270 mRNA. Translation: AAC49041.1 .
    Z66559 mRNA. Translation: CAA91448.1 .

    3D structure databases

    ProteinModelPortali P43310.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.1280.10. 1 hit.
    InterProi IPR016213. Polyphenol_oxidase.
    IPR022740. Polyphenol_oxidase_C.
    IPR022739. Polyphenol_oxidase_cen.
    IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view ]
    Pfami PF12142. PPO1_DWL. 1 hit.
    PF12143. PPO1_KFDV. 1 hit.
    PF00264. Tyrosinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000290. PPO_plant. 1 hit.
    PRINTSi PR00092. TYROSINASE.
    SUPFAMi SSF48056. SSF48056. 1 hit.
    PROSITEi PS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Spinach thylakoid polyphenol oxidase: cloning, characterization, and relation to a putative protein kinase."
      Hind G., Marshak D.R., Coughlan S.J.
      Biochemistry 34:8157-8164(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Hybrid 424.
      Tissue: Leaf.

    Entry informationi

    Entry nameiPPO_SPIOL
    AccessioniPrimary (citable) accession number: P43310
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3