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Protein

Translocon-associated protein subunit alpha

Gene

SSR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.

GO - Biological processi

  • cotranslational protein targeting to membrane Source: ProtInc
  • IRE1-mediated unfolded protein response Source: Reactome
  • positive regulation of cell proliferation Source: ProtInc
Complete GO annotation...

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BioCyciZFISH:ENSG00000124783-MONOMER.
ReactomeiR-HSA-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-HSA-381038. XBP1(S) activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Translocon-associated protein subunit alpha
Short name:
TRAP-alpha
Alternative name(s):
Signal sequence receptor subunit alpha
Short name:
SSR-alpha
Gene namesi
Name:SSR1
Synonyms:TRAPA
ORF Names:PSEC0262
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:11323. SSR1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 207LumenalSequence analysisAdd BLAST189
Transmembranei208 – 228HelicalSequence analysisAdd BLAST21
Topological domaini229 – 286CytoplasmicSequence analysisAdd BLAST58

GO - Cellular componenti

  • endoplasmic reticulum Source: HPA
  • endoplasmic reticulum membrane Source: Reactome
  • integral component of membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6745.
OpenTargetsiENSG00000124783.
PharmGKBiPA36147.

Polymorphism and mutation databases

BioMutaiSSR1.
DMDMi22261821.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 181 PublicationAdd BLAST18
ChainiPRO_000003328119 – 286Translocon-associated protein subunit alphaAdd BLAST268

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi136N-linked (GlcNAc...)2 Publications1
Glycosylationi191N-linked (GlcNAc...)1 Publication1
Modified residuei247PhosphoserineCombined sources1
Modified residuei260PhosphothreonineCombined sources1
Modified residuei268PhosphoserineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP43307.
MaxQBiP43307.
PaxDbiP43307.
PeptideAtlasiP43307.
PRIDEiP43307.
TopDownProteomicsiP43307-1. [P43307-1]
P43307-2. [P43307-2]

PTM databases

iPTMnetiP43307.
PhosphoSitePlusiP43307.

Expressioni

Gene expression databases

BgeeiENSG00000124783.
ExpressionAtlasiP43307. baseline and differential.
GenevisibleiP43307. HS.

Organism-specific databases

HPAiHPA011276.
HPA017062.

Interactioni

Subunit structurei

Heterotetramer of TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma. Interacts with palmitoylated calnexin (CALX), the interaction is required for efficient folding of glycosylated proteins.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CANXP278245EBI-714168,EBI-355947
SERPINA1P010094EBI-714168,EBI-986224

Protein-protein interaction databases

BioGridi112623. 68 interactors.
IntActiP43307. 31 interactors.
MINTiMINT-1379984.
STRINGi9606.ENSP00000244763.

Structurei

3D structure databases

ProteinModelPortaliP43307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Shows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged.

Sequence similaritiesi

Belongs to the TRAP-alpha family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1631. Eukaryota.
ENOG410XNTM. LUCA.
GeneTreeiENSGT00400000022103.
HOVERGENiHBG009736.
InParanoidiP43307.
KOiK13249.
PhylomeDBiP43307.
TreeFamiTF321074.

Family and domain databases

InterProiIPR005595. TRAP_alpha.
[Graphical view]
PfamiPF03896. TRAP_alpha. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P43307-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLLPRLLLL LLLVFPATVL FRGGPRGLLA VAQDLTEDEE TVEDSIIEDE
60 70 80 90 100
DDEAEVEEDE PTDLVEDKEE EDVSGEPEAS PSADTTILFV KGEDFPANNI
110 120 130 140 150
VKFLVGFTNK GTEDFIVESL DASFRYPQDY QFYIQNFTAL PLNTVVPPQR
160 170 180 190 200
QATFEYSFIP AEPMGGRPFG LVINLNYKDL NGNVFQDAVF NQTVTVIERE
210 220 230 240 250
DGLDGETIFM YMFLAGLGLL VIVGLHQLLE SRKRKRPIQK VEMGTSSQND
260 270 280
VDMSWIPQET LNQINKASPR RLPRKRAQKR SVGSDE
Length:286
Mass (Da):32,235
Last modified:August 13, 2002 - v3
Checksum:i2C631DC0CC3EB489
GO
Isoform 2 (identifier: P43307-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     68-94: Missing.

Note: No experimental confirmation available.
Show »
Length:259
Mass (Da):29,373
Checksum:iD79F40EE756B1CB5
GO

Sequence cautioni

The sequence CAI16444 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6R → G in BAC11701 (PubMed:16303743).Curated1
Sequence conflicti37E → A in BAD96529 (Ref. 6) Curated1
Sequence conflicti130Y → H in CAA78290 (PubMed:8050590).Curated1
Sequence conflicti190F → Y in BAF84239 (PubMed:14702039).Curated1
Sequence conflicti220L → P in BAD96529 (Ref. 6) Curated1
Sequence conflicti263Q → R in BAF84239 (PubMed:14702039).Curated1
Sequence conflicti286E → D in CAG33242 (Ref. 5) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02242728L → S.2 PublicationsCorresponds to variant rs10004dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01362168 – 94Missing in isoform 2. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12830 mRNA. Translation: CAA78290.1.
AF156965 mRNA. Translation: AAD48778.1.
BT007387 mRNA. Translation: AAP36051.1.
AK291550 mRNA. Translation: BAF84239.1.
CR456961 mRNA. Translation: CAG33242.1.
AK222762 mRNA. Translation: BAD96482.1.
AK222809 mRNA. Translation: BAD96529.1.
AK075562 mRNA. Translation: BAC11701.1.
AL139095 Genomic DNA. Translation: CAI16444.1. Sequence problems.
BC007710 mRNA. Translation: AAH07710.1.
CCDSiCCDS4499.1. [P43307-1]
PIRiI38246.
RefSeqiNP_001278937.1. NM_001292008.1.
NP_003135.2. NM_003144.4. [P43307-1]
UniGeneiHs.114033.

Genome annotation databases

EnsembliENST00000244763; ENSP00000244763; ENSG00000124783. [P43307-1]
GeneIDi6745.
KEGGihsa:6745.
UCSCiuc003mxf.6. human. [P43307-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12830 mRNA. Translation: CAA78290.1.
AF156965 mRNA. Translation: AAD48778.1.
BT007387 mRNA. Translation: AAP36051.1.
AK291550 mRNA. Translation: BAF84239.1.
CR456961 mRNA. Translation: CAG33242.1.
AK222762 mRNA. Translation: BAD96482.1.
AK222809 mRNA. Translation: BAD96529.1.
AK075562 mRNA. Translation: BAC11701.1.
AL139095 Genomic DNA. Translation: CAI16444.1. Sequence problems.
BC007710 mRNA. Translation: AAH07710.1.
CCDSiCCDS4499.1. [P43307-1]
PIRiI38246.
RefSeqiNP_001278937.1. NM_001292008.1.
NP_003135.2. NM_003144.4. [P43307-1]
UniGeneiHs.114033.

3D structure databases

ProteinModelPortaliP43307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112623. 68 interactors.
IntActiP43307. 31 interactors.
MINTiMINT-1379984.
STRINGi9606.ENSP00000244763.

PTM databases

iPTMnetiP43307.
PhosphoSitePlusiP43307.

Polymorphism and mutation databases

BioMutaiSSR1.
DMDMi22261821.

Proteomic databases

EPDiP43307.
MaxQBiP43307.
PaxDbiP43307.
PeptideAtlasiP43307.
PRIDEiP43307.
TopDownProteomicsiP43307-1. [P43307-1]
P43307-2. [P43307-2]

Protocols and materials databases

DNASUi6745.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000244763; ENSP00000244763; ENSG00000124783. [P43307-1]
GeneIDi6745.
KEGGihsa:6745.
UCSCiuc003mxf.6. human. [P43307-1]

Organism-specific databases

CTDi6745.
DisGeNETi6745.
GeneCardsiSSR1.
HGNCiHGNC:11323. SSR1.
HPAiHPA011276.
HPA017062.
MIMi600868. gene.
neXtProtiNX_P43307.
OpenTargetsiENSG00000124783.
PharmGKBiPA36147.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1631. Eukaryota.
ENOG410XNTM. LUCA.
GeneTreeiENSGT00400000022103.
HOVERGENiHBG009736.
InParanoidiP43307.
KOiK13249.
PhylomeDBiP43307.
TreeFamiTF321074.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000124783-MONOMER.
ReactomeiR-HSA-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-HSA-381038. XBP1(S) activates chaperone genes.

Miscellaneous databases

ChiTaRSiSSR1. human.
GeneWikiiSSR1.
GenomeRNAii6745.
PROiP43307.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000124783.
ExpressionAtlasiP43307. baseline and differential.
GenevisibleiP43307. HS.

Family and domain databases

InterProiIPR005595. TRAP_alpha.
[Graphical view]
PfamiPF03896. TRAP_alpha. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSSRA_HUMAN
AccessioniPrimary (citable) accession number: P43307
Secondary accession number(s): A8K685
, Q53GX2, Q53H19, Q5TAM3, Q6IB43, Q8NBH9, Q96IA2, Q9TNQ8, Q9UN49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 13, 2002
Last modified: November 30, 2016
This is version 154 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Seems to bind calcium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.