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Protein

DNA replication licensing factor MCM7

Gene

MCM7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable component of the MCM2-7 complex (MCM complex) that may function as a DNA helicase and which is essential to undergo a single round of replication initiation and elongation per cell cycle in eukaryotic cells (By similarity). Required for megagametophyte and embryo development.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri178 – 205C4-typeBy similarityAdd BLAST28
Nucleotide bindingi376 – 383ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • DNA replication initiation Source: InterPro
  • DNA unwinding involved in DNA replication Source: TAIR
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • sugar mediated signaling pathway Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-68867. Assembly of the pre-replicative complex.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-68962. Activation of the pre-replicative complex.
R-ATH-69052. Switching of origins to a post-replicative state.
R-ATH-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM7 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance protein 7
Short name:
AtMCM7
Protein PROLIFERA
Gene namesi
Name:MCM7
Synonyms:PRL
Ordered Locus Names:At4g02060
ORF Names:AGAA.2, T10M13.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G02060.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • MCM complex Source: InterPro
  • nucleus Source: TAIR
  • THO complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941221 – 716DNA replication licensing factor MCM7Add BLAST716

Proteomic databases

PaxDbiP43299.
PRIDEiP43299.

PTM databases

iPTMnetiP43299.

Expressioni

Tissue specificityi

Expressed in shoot apex and flower buds.1 Publication

Developmental stagei

Accumulates in nucleus during the G1 phase of the cell cycle. During mitosis, the protein rapidly disappears, returning to daughter nuclei during G1.

Inductioni

By both rootknot and cyst nematode infections.1 Publication

Gene expression databases

GenevisibleiP43299. AT.

Interactioni

Subunit structurei

Component of the minichromosome maintenance (MCM) complex, a heterotetramer composed of MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7. Interacts with ETG1.1 Publication

Protein-protein interaction databases

BioGridi13442. 5 interactors.
IntActiP43299. 5 interactors.
MINTiMINT-6799339.
STRINGi3702.AT4G02060.1.

Structurei

3D structure databases

ProteinModelPortaliP43299.
SMRiP43299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini326 – 531MCMAdd BLAST206

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi508 – 511Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri178 – 205C4-typeBy similarityAdd BLAST28

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0482. Eukaryota.
COG1241. LUCA.
HOGENOMiHOG000224125.
InParanoidiP43299.
KOiK02210.
OMAiTCDRCGC.
OrthoDBiEOG093603EB.
PhylomeDBiP43299.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR008050. MCM7.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR033762. MCM_OB.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11630:SF26. PTHR11630:SF26. 2 hits.
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
PF17207. MCM_OB. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01663. MCMPROTEIN7.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDHDFDGDK GLAKGFLENF ADANGRSKYM EILQEVSNRK IRAIQVDLDD
60 70 80 90 100
LFNYKDESEE FLGRLTENTR RYVSIFSAAV DELLPEPTEA FPDDDHDILM
110 120 130 140 150
TQRADDGTDN PDVSDPHQQI PSEIKRYYEV YFKAPSKGRP STIREVKASH
160 170 180 190 200
IGQLVRISGI VTRCSDVKPL MAVAVYTCED CGHEIYQEVT SRVFMPLFKC
210 220 230 240 250
PSSRCRLNSK AGNPILQLRA SKFLKFQEAK MQELAEHVPK GHIPRSMTVH
260 270 280 290 300
LRGELTRKVS PGDVVEFSGI FLPIPYTGFK ALRAGLVADT YLEATSVTHF
310 320 330 340 350
KKKYEEYEFQ KDEEEQIARL AEDGDIYNKL SRSLAPEIYG HEDIKKALLL
360 370 380 390 400
LLVGAPHRQL KDGMKIRGDV HICLMGDPGV AKSQLLKHII NVAPRGVYTT
410 420 430 440 450
GKGSSGVGLT AAVMRDQVTN EMVLEGGALV LADMGICAID EFDKMDESDR
460 470 480 490 500
TAIHEVMEQQ TVSIAKAGIT TSLNARTAVL AAANPAWGRY DLRRTPAENI
510 520 530 540 550
NLPPALLSRF DLLWLILDRA DMDSDLELAK HVLHVHQTEE SPALGFEPLE
560 570 580 590 600
PNILRAYISA ARRLSPYVPA ELEEYIATAY SSIRQEEAKS NTPHSYTTVR
610 620 630 640 650
TLLSILRISA ALARLRFSES VAQSDVDEAL RLMQMSKISL YADDRQKAGL
660 670 680 690 700
DAISDTYSII RDEAARSKKT HVSYANALNW ISRKGYSEAQ LKECLEEYAA
710
LNVWQIDPHT FDIRFI
Length:716
Mass (Da):80,333
Last modified:January 23, 2002 - v2
Checksum:i102DE5445E0C32D5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti707D → E in AAC37429 (PubMed:7754372).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39954 mRNA. Translation: AAC37429.1.
AF001535 Genomic DNA. Translation: AAB57797.1.
AF001308 Genomic DNA. Translation: AAC78698.1.
AL161493 Genomic DNA. Translation: CAB80699.1.
CP002687 Genomic DNA. Translation: AEE82117.1.
CP002687 Genomic DNA. Translation: AEE82118.1.
PIRiT01507.
RefSeqiNP_001190655.1. NM_001203726.1.
NP_192115.1. NM_116437.3.
UniGeneiAt.3861.

Genome annotation databases

EnsemblPlantsiAT4G02060.1; AT4G02060.1; AT4G02060.
AT4G02060.2; AT4G02060.2; AT4G02060.
GeneIDi828153.
GrameneiAT4G02060.1; AT4G02060.1; AT4G02060.
AT4G02060.2; AT4G02060.2; AT4G02060.
KEGGiath:AT4G02060.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39954 mRNA. Translation: AAC37429.1.
AF001535 Genomic DNA. Translation: AAB57797.1.
AF001308 Genomic DNA. Translation: AAC78698.1.
AL161493 Genomic DNA. Translation: CAB80699.1.
CP002687 Genomic DNA. Translation: AEE82117.1.
CP002687 Genomic DNA. Translation: AEE82118.1.
PIRiT01507.
RefSeqiNP_001190655.1. NM_001203726.1.
NP_192115.1. NM_116437.3.
UniGeneiAt.3861.

3D structure databases

ProteinModelPortaliP43299.
SMRiP43299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13442. 5 interactors.
IntActiP43299. 5 interactors.
MINTiMINT-6799339.
STRINGi3702.AT4G02060.1.

PTM databases

iPTMnetiP43299.

Proteomic databases

PaxDbiP43299.
PRIDEiP43299.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G02060.1; AT4G02060.1; AT4G02060.
AT4G02060.2; AT4G02060.2; AT4G02060.
GeneIDi828153.
GrameneiAT4G02060.1; AT4G02060.1; AT4G02060.
AT4G02060.2; AT4G02060.2; AT4G02060.
KEGGiath:AT4G02060.

Organism-specific databases

TAIRiAT4G02060.

Phylogenomic databases

eggNOGiKOG0482. Eukaryota.
COG1241. LUCA.
HOGENOMiHOG000224125.
InParanoidiP43299.
KOiK02210.
OMAiTCDRCGC.
OrthoDBiEOG093603EB.
PhylomeDBiP43299.

Enzyme and pathway databases

ReactomeiR-ATH-68867. Assembly of the pre-replicative complex.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-68962. Activation of the pre-replicative complex.
R-ATH-69052. Switching of origins to a post-replicative state.
R-ATH-69300. Removal of licensing factors from origins.

Miscellaneous databases

PROiP43299.

Gene expression databases

GenevisibleiP43299. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR008050. MCM7.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR033762. MCM_OB.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11630:SF26. PTHR11630:SF26. 2 hits.
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
PF17207. MCM_OB. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01663. MCMPROTEIN7.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCM7_ARATH
AccessioniPrimary (citable) accession number: P43299
Secondary accession number(s): O04721
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2002
Last modified: November 30, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.