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Protein

DNA replication licensing factor MCM7

Gene

MCM7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable component of the MCM2-7 complex (MCM complex) that may function as a DNA helicase and which is essential to undergo a single round of replication initiation and elongation per cell cycle in eukaryotic cells (By similarity). Required for megagametophyte and embryo development.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri178 – 205C4-typeBy similarityAdd BLAST28
Nucleotide bindingi376 – 383ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • DNA replication initiation Source: InterPro
  • DNA unwinding involved in DNA replication Source: TAIR
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • sugar mediated signaling pathway Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication, Transcription, Transcription regulation
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-68867 Assembly of the pre-replicative complex
R-ATH-68949 Orc1 removal from chromatin
R-ATH-68962 Activation of the pre-replicative complex
R-ATH-69052 Switching of origins to a post-replicative state

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM7 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance protein 7
Short name:
AtMCM7
Protein PROLIFERA
Gene namesi
Name:MCM7
Synonyms:PRL
Ordered Locus Names:At4g02060
ORF Names:AGAA.2, T10M13.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G02060
TAIRilocus:2132223 AT4G02060

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941221 – 716DNA replication licensing factor MCM7Add BLAST716

Proteomic databases

PaxDbiP43299

PTM databases

iPTMnetiP43299

Expressioni

Tissue specificityi

Expressed in shoot apex and flower buds.1 Publication

Developmental stagei

Accumulates in nucleus during the G1 phase of the cell cycle. During mitosis, the protein rapidly disappears, returning to daughter nuclei during G1.

Inductioni

By both rootknot and cyst nematode infections.1 Publication

Gene expression databases

ExpressionAtlasiP43299 baseline and differential
GenevisibleiP43299 AT

Interactioni

Subunit structurei

Component of the minichromosome maintenance (MCM) complex, a heterotetramer composed of MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7. Interacts with ETG1.1 Publication

Protein-protein interaction databases

BioGridi13442, 5 interactors
IntActiP43299, 5 interactors
MINTiP43299
STRINGi3702.AT4G02060.1

Structurei

3D structure databases

ProteinModelPortaliP43299
SMRiP43299
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini326 – 531MCMAdd BLAST206

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi508 – 511Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri178 – 205C4-typeBy similarityAdd BLAST28

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0482 Eukaryota
COG1241 LUCA
HOGENOMiHOG000224125
InParanoidiP43299
KOiK02210
OMAiVKIQEMA
OrthoDBiEOG093603EB
PhylomeDBiP43299

Family and domain databases

InterProiView protein in InterPro
IPR031327 MCM
IPR008050 MCM7
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PTHR11630:SF26 PTHR11630:SF26, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01663 MCMPROTEIN7
SMARTiView protein in SMART
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 2 hits
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

P43299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDHDFDGDK GLAKGFLENF ADANGRSKYM EILQEVSNRK IRAIQVDLDD
60 70 80 90 100
LFNYKDESEE FLGRLTENTR RYVSIFSAAV DELLPEPTEA FPDDDHDILM
110 120 130 140 150
TQRADDGTDN PDVSDPHQQI PSEIKRYYEV YFKAPSKGRP STIREVKASH
160 170 180 190 200
IGQLVRISGI VTRCSDVKPL MAVAVYTCED CGHEIYQEVT SRVFMPLFKC
210 220 230 240 250
PSSRCRLNSK AGNPILQLRA SKFLKFQEAK MQELAEHVPK GHIPRSMTVH
260 270 280 290 300
LRGELTRKVS PGDVVEFSGI FLPIPYTGFK ALRAGLVADT YLEATSVTHF
310 320 330 340 350
KKKYEEYEFQ KDEEEQIARL AEDGDIYNKL SRSLAPEIYG HEDIKKALLL
360 370 380 390 400
LLVGAPHRQL KDGMKIRGDV HICLMGDPGV AKSQLLKHII NVAPRGVYTT
410 420 430 440 450
GKGSSGVGLT AAVMRDQVTN EMVLEGGALV LADMGICAID EFDKMDESDR
460 470 480 490 500
TAIHEVMEQQ TVSIAKAGIT TSLNARTAVL AAANPAWGRY DLRRTPAENI
510 520 530 540 550
NLPPALLSRF DLLWLILDRA DMDSDLELAK HVLHVHQTEE SPALGFEPLE
560 570 580 590 600
PNILRAYISA ARRLSPYVPA ELEEYIATAY SSIRQEEAKS NTPHSYTTVR
610 620 630 640 650
TLLSILRISA ALARLRFSES VAQSDVDEAL RLMQMSKISL YADDRQKAGL
660 670 680 690 700
DAISDTYSII RDEAARSKKT HVSYANALNW ISRKGYSEAQ LKECLEEYAA
710
LNVWQIDPHT FDIRFI
Length:716
Mass (Da):80,333
Last modified:January 23, 2002 - v2
Checksum:i102DE5445E0C32D5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti707D → E in AAC37429 (PubMed:7754372).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39954 mRNA Translation: AAC37429.1
AF001535 Genomic DNA Translation: AAB57797.1
AF001308 Genomic DNA Translation: AAC78698.1
AL161493 Genomic DNA Translation: CAB80699.1
CP002687 Genomic DNA Translation: AEE82117.1
CP002687 Genomic DNA Translation: AEE82118.1
PIRiT01507
RefSeqiNP_001190655.1, NM_001203726.1
NP_192115.1, NM_116437.3
UniGeneiAt.3861

Genome annotation databases

EnsemblPlantsiAT4G02060.1; AT4G02060.1; AT4G02060
AT4G02060.2; AT4G02060.2; AT4G02060
GeneIDi828153
GrameneiAT4G02060.1; AT4G02060.1; AT4G02060
AT4G02060.2; AT4G02060.2; AT4G02060
KEGGiath:AT4G02060

Similar proteinsi

Entry informationi

Entry nameiMCM7_ARATH
AccessioniPrimary (citable) accession number: P43299
Secondary accession number(s): O04721
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2002
Last modified: May 23, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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