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Protein

Cysteine proteinase RD21A

Gene

RD21A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cysteine protease that plays a role in immunity, senescence, and biotic and abiotic stresses (Probable). Involved in immunity against the necrotrophic fungal pathogen Botrytis cinerea (PubMed:22238602). Involved in elicitor-stimulated programmed cell death (PCD). During infection by the necrotrophic fungal pathogen Botrytis cinerea, functions as PCD-promoting protease that is released from the ER body or vacuole to the cytoplasm (PubMed:23398119). Accumulates in endoplasmic reticulum-derived bodies in epidermal cells and may participate in cell death in stressed or injured cells (PubMed:11577182). Involved in water stress-induced cell death through its protease activity that is released to the cytoplasm after vacuolar collapse (PubMed:26884487). Possesses protease activity in vitro and is involved in cell death in the transmitting tract and septum epidermis during flower development (PubMed:26160583). Possesses peptide ligase activity. Can ligate peptides to unmodified N-termini of acceptor proteins. Probably ligates through a thioester intermediate (PubMed:18660805).1 Publication6 Publications

Enzyme regulationi

Inhibited by the cysteine protease inhibitor E-64.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei161PROSITE-ProRule annotation1 Publication1
Active sitei297PROSITE-ProRule annotation1 Publication1
Active sitei317PROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

  • cysteine-type endopeptidase activity Source: GO_Central
  • cysteine-type peptidase activity Source: TAIR
  • peptidase activity Source: TAIR

GO - Biological processi

  • defense response to fungus Source: TAIR
  • proteolysis Source: TAIR
  • proteolysis involved in cellular protein catabolic process Source: GO_Central

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Protein family/group databases

MEROPSiC01.064

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine proteinase RD21ACurated (EC:3.4.22.-Curated)
Alternative name(s):
Protein RESPONSIVE TO DEHYDRATION 211 Publication
Short name:
RD211 Publication
Gene namesi
Name:RD21A1 Publication
Ordered Locus Names:At1g47128
ORF Names:F2G19.31
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G47128
TAIRilocus:2825832 AT1G47128

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Vacuole

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions (PubMed:22238602, PubMed:22396764). Mutant plants show increased susceptibility to infection by the necrotrophic fungal pathogen Botrytis cinerea (PubMed:22238602).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi161C → A: Loss of protease activity. 1 Publication1
Mutagenesisi297H → A: Loss of protease activity. 1 Publication1
Mutagenesisi317N → A: Reduces protease activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000002645722 – 136Activation peptide1 PublicationAdd BLAST115
ChainiPRO_0000026458137 – 352Cysteine proteinase RD21AAdd BLAST216
PropeptideiPRO_0000046018353 – 462Removed in mature form1 PublicationAdd BLAST110

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi90N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi158 ↔ 200By similarity
Disulfide bondi192 ↔ 233By similarity
Disulfide bondi291 ↔ 342By similarity
Disulfide bondi375 ↔ 387By similarity
Disulfide bondi381 ↔ 402By similarity
Glycosylationi414N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP43297
PRIDEiP43297

Expressioni

Inductioni

By high salt conditions and drought stress.1 Publication

Gene expression databases

ExpressionAtlasiP43297 baseline and differential
GenevisibleiP43297 AT

Interactioni

Subunit structurei

Interacts with SERPIN1 (PubMed:23398119, PubMed:20181955). Interacts with PRN2 (PubMed:24947605). Interacts with WSCP (PubMed:26160583, PubMed:26016527).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PDIL1-1Q9XI014EBI-1993101,EBI-449394

Protein-protein interaction databases

BioGridi26347, 11 interactors
IntActiP43297, 1 interactor
STRINGi3702.AT1G47128.1

Structurei

3D structure databases

ProteinModelPortaliP43297
SMRiP43297
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543 Eukaryota
KOG4296 Eukaryota
COG4870 LUCA
HOGENOMiHOG000230773
InParanoidiP43297
OMAiNANDMFY
OrthoDBiEOG093609HI
PhylomeDBiP43297

Family and domain databases

Gene3Di3.10.20.500, 1 hit
InterProiView protein in InterPro
IPR000118 Granulin
IPR037277 Granulin_sf
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF00396 Granulin, 1 hit
PF08246 Inhibitor_I29, 1 hit
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00277 GRAN, 1 hit
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43297-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFLKPTMAI LFLAMVAVSS AVDMSIISYD EKHGVSTTGG RSEAEVMSIY
60 70 80 90 100
EAWLVKHGKA QSQNSLVEKD RRFEIFKDNL RFVDEHNEKN LSYRLGLTRF
110 120 130 140 150
ADLTNDEYRS KYLGAKMEKK GERRTSLRYE ARVGDELPES IDWRKKGAVA
160 170 180 190 200
EVKDQGGCGS CWAFSTIGAV EGINQIVTGD LITLSEQELV DCDTSYNEGC
210 220 230 240 250
NGGLMDYAFE FIIKNGGIDT DKDYPYKGVD GTCDQIRKNA KVVTIDSYED
260 270 280 290 300
VPTYSEESLK KAVAHQPISI AIEAGGRAFQ LYDSGIFDGS CGTQLDHGVV
310 320 330 340 350
AVGYGTENGK DYWIVRNSWG KSWGESGYLR MARNIASSSG KCGIAIEPSY
360 370 380 390 400
PIKNGENPPN PGPSPPSPIK PPTQCDSYYT CPESNTCCCL FEYGKYCFAW
410 420 430 440 450
GCCPLEAATC CDDNYSCCPH EYPVCDLDQG TCLLSKNSPF SVKALKRKPA
460
TPFWSQGRKN IA
Length:462
Mass (Da):50,966
Last modified:November 1, 1995 - v1
Checksum:i4C59955CCB95AA58
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13043 Genomic DNA Translation: BAA02374.1
AC083835 Genomic DNA Translation: AAG50628.1
CP002684 Genomic DNA Translation: AEE32135.1
AY072130 mRNA Translation: AAL59952.1
AY133781 mRNA Translation: AAM91715.1
PIRiJN0719
RefSeqiNP_564497.1, NM_103612.3
UniGeneiAt.43549
At.47599
At.71705

Genome annotation databases

EnsemblPlantsiAT1G47128.1; AT1G47128.1; AT1G47128
GeneIDi841122
GrameneiAT1G47128.1; AT1G47128.1; AT1G47128
KEGGiath:AT1G47128

Similar proteinsi

Entry informationi

Entry nameiRD21A_ARATH
AccessioniPrimary (citable) accession number: P43297
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 25, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health