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Protein

Probable cysteine protease RD19B

Gene

RD19B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Probable thiol protease.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei156 – 1561PROSITE-ProRule annotation
Active sitei299 – 2991PROSITE-ProRule annotation
Active sitei326 – 3261PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciARA:AT2G21430-MONOMER.
ReactomeiR-ATH-114608. Platelet degranulation.
R-ATH-2132295. MHC class II antigen presentation.

Protein family/group databases

MEROPSiC01.A04.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cysteine protease RD19BCurated (EC:3.4.22.-)
Alternative name(s):
RD19-like protein 11 Publication
Thiol protease A14941 Publication
Gene namesi
Name:RD19BCurated
Synonyms:RDL11 Publication
Ordered Locus Names:At2g21430
ORF Names:F3K23.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G21430.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Propeptidei25 – 131107Activation peptideCuratedPRO_0000026453Add
BLAST
Chaini132 – 361230Probable cysteine protease RD19BPRO_0000026454Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi153 ↔ 203By similarity
Disulfide bondi187 ↔ 237By similarity
Glycosylationi250 – 2501N-linked (GlcNAc...)PROSITE-ProRule annotation
Disulfide bondi293 ↔ 347By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP43295.
PRIDEiP43295.

Expressioni

Inductioni

By wilting and abscisic acid (ABA) (PubMed:8018874). Induced by drought stress (PubMed:12102506).2 Publications

Gene expression databases

ExpressionAtlasiP43295. baseline and differential.
GenevisibleiP43295. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G21430.1.

Structurei

3D structure databases

ProteinModelPortaliP43295.
SMRiP43295. Positions 43-359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1542. Eukaryota.
COG4870. LUCA.
HOGENOMiHOG000230774.
InParanoidiP43295.
KOiK01373.
OMAiGSIEEHY.
PhylomeDBiP43295.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYHLRVLFS VSLIFVFVSV SVCGDEDVLI RQVVDETEPK VLSSEDHFTL
60 70 80 90 100
FKKKFGKVYG SIEEHYYRFS VFKANLLRAM RHQKMDPSAR HGVTQFSDLT
110 120 130 140 150
RSEFRRKHLG VKGGFKLPKD ANQAPILPTQ NLPEEFDWRD RGAVTPVKNQ
160 170 180 190 200
GSCGSCWSFS TTGALEGAHF LATGKLVSLS EQQLVDCDHE CDPEEEGSCD
210 220 230 240 250
SGCNGGLMNS AFEYTLKTGG LMREKDYPYT GTDGGSCKLD RSKIVASVSN
260 270 280 290 300
FSVVSINEDQ IAANLIKNGP LAVAINAAYM QTYIGGVSCP YICSRRLNHG
310 320 330 340 350
VLLVGYGSAG FSQARLKEKP YWIIKNSWGE SWGENGFYKI CKGRNICGVD
360
SLVSTVAATT S
Length:361
Mass (Da):39,819
Last modified:December 8, 2000 - v2
Checksum:iD09A2CE5654642DD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491T → A in CAA52403 (PubMed:8018874).Curated
Sequence conflicti206 – 2061G → R in BAD43619 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006841 Genomic DNA. Translation: AAD23687.1.
CP002685 Genomic DNA. Translation: AEC07177.1.
AK175856 mRNA. Translation: BAD43619.1.
BT029231 mRNA. Translation: ABJ98563.1.
X74359 mRNA. Translation: CAA52403.1.
PIRiB84601.
RefSeqiNP_565512.1. NM_127715.3.
UniGeneiAt.14069.

Genome annotation databases

EnsemblPlantsiAT2G21430.1; AT2G21430.1; AT2G21430.
GeneIDi816682.
GrameneiAT2G21430.1; AT2G21430.1; AT2G21430.
KEGGiath:AT2G21430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006841 Genomic DNA. Translation: AAD23687.1.
CP002685 Genomic DNA. Translation: AEC07177.1.
AK175856 mRNA. Translation: BAD43619.1.
BT029231 mRNA. Translation: ABJ98563.1.
X74359 mRNA. Translation: CAA52403.1.
PIRiB84601.
RefSeqiNP_565512.1. NM_127715.3.
UniGeneiAt.14069.

3D structure databases

ProteinModelPortaliP43295.
SMRiP43295. Positions 43-359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G21430.1.

Protein family/group databases

MEROPSiC01.A04.

Proteomic databases

PaxDbiP43295.
PRIDEiP43295.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G21430.1; AT2G21430.1; AT2G21430.
GeneIDi816682.
GrameneiAT2G21430.1; AT2G21430.1; AT2G21430.
KEGGiath:AT2G21430.

Organism-specific databases

TAIRiAT2G21430.

Phylogenomic databases

eggNOGiKOG1542. Eukaryota.
COG4870. LUCA.
HOGENOMiHOG000230774.
InParanoidiP43295.
KOiK01373.
OMAiGSIEEHY.
PhylomeDBiP43295.

Enzyme and pathway databases

BioCyciARA:AT2G21430-MONOMER.
ReactomeiR-ATH-114608. Platelet degranulation.
R-ATH-2132295. MHC class II antigen presentation.

Miscellaneous databases

PROiP43295.

Gene expression databases

ExpressionAtlasiP43295. baseline and differential.
GenevisibleiP43295. AT.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Arabidopsis ORF Clones."
    Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Characterization of a cDNA from Arabidopsis thaliana encoding a potential thiol protease whose expression is induced independently by wilting and abscisic acid."
    Williams J., Bulman M., Huttly A.K., Phillips A., Neill S.
    Plant Mol. Biol. 25:259-270(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 49-361, INDUCTION.
    Strain: cv. Landsberg erecta.
  6. "Classification of genes differentially expressed during water-deficit stress in Arabidopsis thaliana: an analysis using microarray and differential expression data."
    Bray E.A.
    Ann. Bot. 89:803-811(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY DROUGHT STRESS.
  7. "RD19, an Arabidopsis cysteine protease required for RRS1-R-mediated resistance, is relocalized to the nucleus by the Ralstonia solanacearum PopP2 effector."
    Bernoux M., Timmers T., Jauneau A., Briere C., de Wit P.J., Marco Y., Deslandes L.
    Plant Cell 20:2252-2264(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiRD19B_ARATH
AccessioniPrimary (citable) accession number: P43295
Secondary accession number(s): Q058I7, Q680L1, Q9SJT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 8, 2000
Last modified: July 6, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.