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P43294

- MHK_ARATH

UniProt

P43294 - MHK_ARATH

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Protein
Serine/threonine-protein kinase MHK
Gene
MHK, At4g13020, F25G13.110
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

May play a role in the regulation of plant growth and development.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATP By similarity
Active sitei133 – 1331Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G13020-MONOMER.
ARA:GQT-448-MONOMER.
ARA:GQT-597-MONOMER.
ARA:GQT-598-MONOMER.
ARA:GQT-599-MONOMER.
BRENDAi2.7.11.22. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase MHK (EC:2.7.11.22)
Gene namesi
Name:MHK
Ordered Locus Names:At4g13020
ORF Names:F25G13.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G13020.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443Serine/threonine-protein kinase MHK
PRO_0000086325Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei164 – 1641Phosphothreonine By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Roots, leaves and stems.

Gene expression databases

ArrayExpressiP43294.
GenevestigatoriP43294.

Interactioni

Protein-protein interaction databases

BioGridi12212. 1 interaction.
IntActiP43294. 9 interactions.

Structurei

3D structure databases

ProteinModelPortaliP43294.
SMRiP43294. Positions 11-341.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 291280Protein kinase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiP43294.
PhylomeDBiP43294.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P43294-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MMVFVVFVMC RYKILEELGD GTCGSVYKAV NLETYEVVAV KKMKRKFYYW    50
EECVNLREVK ALRKLNHPHI IKLKEIVREH NELFFIFECM DHNLYHIMKE 100
RERPFSEGEI RSFMSQMLQG LAHMHKNGYF HRDLKPENLL VTNNILKIAD 150
FGLAREVASM PPYTEYVSTR WYRAPEVLLQ SSLYTPAVDM WAVGAILAEL 200
YALTPLFPGE SEIDQLYKIC CVLGKPDWTT FPEAKSISRI MSISHTEFPQ 250
TRIADLLPNA APEAIDLINR LCSWDPLKRP TADEALNHPF FSMATQASYP 300
IHDLELRLDN MAALPNLELN LWDFNREPEE CFLGLTLAVK PSAPKLEMLR 350
NVSQDMSENF LFCPGVNNDR EPSVFWSLLS PDENGLHAPV ESSPLSLSFS 400
PMQQHTVGPP QSSGFTMTSS MQPNMLDRPW MAVSAPFQQS HYL 443
Length:443
Mass (Da):50,896
Last modified:June 6, 2002 - v2
Checksum:iAF92A2233B673F0F
GO
Isoform Short (identifier: P43294-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MMVFVVFVMC → ME

Show »
Length:435
Mass (Da):49,968
Checksum:iE4837811577EB337
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1010MMVFVVFVMC → ME in isoform Short.
VSP_004861

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661N → K in AAA18854. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07249 mRNA. Translation: AAA18854.1.
AL079349 Genomic DNA. Translation: CAB45501.1.
AL161535 Genomic DNA. Translation: CAB78344.1.
CP002687 Genomic DNA. Translation: AEE83214.1.
CP002687 Genomic DNA. Translation: AEE83215.1.
AF360324 mRNA. Translation: AAK26034.1.
AY056332 mRNA. Translation: AAL07181.1.
PIRiC85140.
S38327.
RefSeqiNP_193038.1. NM_117371.1. [P43294-1]
NP_849370.1. NM_179039.3. [P43294-2]
UniGeneiAt.236.

Genome annotation databases

EnsemblPlantsiAT4G13020.2; AT4G13020.2; AT4G13020. [P43294-1]
GeneIDi826915.
KEGGiath:AT4G13020.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07249 mRNA. Translation: AAA18854.1 .
AL079349 Genomic DNA. Translation: CAB45501.1 .
AL161535 Genomic DNA. Translation: CAB78344.1 .
CP002687 Genomic DNA. Translation: AEE83214.1 .
CP002687 Genomic DNA. Translation: AEE83215.1 .
AF360324 mRNA. Translation: AAK26034.1 .
AY056332 mRNA. Translation: AAL07181.1 .
PIRi C85140.
S38327.
RefSeqi NP_193038.1. NM_117371.1. [P43294-1 ]
NP_849370.1. NM_179039.3. [P43294-2 ]
UniGenei At.236.

3D structure databases

ProteinModelPortali P43294.
SMRi P43294. Positions 11-341.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 12212. 1 interaction.
IntActi P43294. 9 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G13020.2 ; AT4G13020.2 ; AT4G13020 . [P43294-1 ]
GeneIDi 826915.
KEGGi ath:AT4G13020.

Organism-specific databases

TAIRi AT4G13020.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi P43294.
PhylomeDBi P43294.

Enzyme and pathway databases

BioCyci ARA:AT4G13020-MONOMER.
ARA:GQT-448-MONOMER.
ARA:GQT-597-MONOMER.
ARA:GQT-598-MONOMER.
ARA:GQT-599-MONOMER.
BRENDAi 2.7.11.22. 399.

Gene expression databases

ArrayExpressi P43294.
Genevestigatori P43294.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of two novel protein kinase genes from Arabidopsis thaliana."
    Moran T.V., Walker J.C.
    Biochim. Biophys. Acta 1216:9-14(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], ALTERNATIVE SPLICING.
    Strain: cv. Columbia.

Entry informationi

Entry nameiMHK_ARATH
AccessioniPrimary (citable) accession number: P43294
Secondary accession number(s): Q9C5D4, Q9SV67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 6, 2002
Last modified: May 14, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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