P43294 (MHK_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase MHK
EC=2.7.11.22

Gene names

Name:	MHK
Ordered Locus Names:	At4g13020
ORF Names:	F25G13.110

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in the regulation of plant growth and development.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Roots, leaves and stems.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P43294-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P43294-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MMVFVVFVMC → ME

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Serine/threonine-protein kinase MHK		PRO_0000086325
Regions
	Domain	12 – 291	280	Protein kinase
	Nucleotide binding	18 – 26	9	ATP By similarity
Sites
	Active site	133	1	Proton acceptor By similarity
	Binding site	41	1	ATP By similarity
Natural variations
	Alternative sequence	1 – 10	10	MMVFVVFVMC → ME in isoform Short.		VSP_004861
Experimental info
	Sequence conflict	66	1	N → K in AAA18854. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified June 6, 2002. Version 2. Checksum: AF92A2233B673F0F	FASTA	443	50,896
	10 20 30 40 50 60 MMVFVVFVMC RYKILEELGD GTCGSVYKAV NLETYEVVAV KKMKRKFYYW EECVNLREVK 70 80 90 100 110 120 ALRKLNHPHI IKLKEIVREH NELFFIFECM DHNLYHIMKE RERPFSEGEI RSFMSQMLQG 130 140 150 160 170 180 LAHMHKNGYF HRDLKPENLL VTNNILKIAD FGLAREVASM PPYTEYVSTR WYRAPEVLLQ 190 200 210 220 230 240 SSLYTPAVDM WAVGAILAEL YALTPLFPGE SEIDQLYKIC CVLGKPDWTT FPEAKSISRI 250 260 270 280 290 300 MSISHTEFPQ TRIADLLPNA APEAIDLINR LCSWDPLKRP TADEALNHPF FSMATQASYP 310 320 330 340 350 360 IHDLELRLDN MAALPNLELN LWDFNREPEE CFLGLTLAVK PSAPKLEMLR NVSQDMSENF 370 380 390 400 410 420 LFCPGVNNDR EPSVFWSLLS PDENGLHAPV ESSPLSLSFS PMQQHTVGPP QSSGFTMTSS 430 440 MQPNMLDRPW MAVSAPFQQS HYL
	Isoform Short [UniParc]. Checksum: E4837811577EB337	FASTA	435	49,968
	10 20 30 40 50 60 MERYKILEEL GDGTCGSVYK AVNLETYEVV AVKKMKRKFY YWEECVNLRE VKALRKLNHP 70 80 90 100 110 120 HIIKLKEIVR EHNELFFIFE CMDHNLYHIM KERERPFSEG EIRSFMSQML QGLAHMHKNG 130 140 150 160 170 180 YFHRDLKPEN LLVTNNILKI ADFGLAREVA SMPPYTEYVS TRWYRAPEVL LQSSLYTPAV 190 200 210 220 230 240 DMWAVGAILA ELYALTPLFP GESEIDQLYK ICCVLGKPDW TTFPEAKSIS RIMSISHTEF 250 260 270 280 290 300 PQTRIADLLP NAAPEAIDLI NRLCSWDPLK RPTADEALNH PFFSMATQAS YPIHDLELRL 310 320 330 340 350 360 DNMAALPNLE LNLWDFNREP EECFLGLTLA VKPSAPKLEM LRNVSQDMSE NFLFCPGVNN 370 380 390 400 410 420 DREPSVFWSL LSPDENGLHA PVESSPLSLS FSPMQQHTVG PPQSSGFTMT SSMQPNMLDR 430 PWMAVSAPFQ QSHYL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of two novel protein kinase genes from Arabidopsis thaliana." Moran T.V., Walker J.C. Biochim. Biophys. Acta 1216:9-14(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], ALTERNATIVE SPLICING. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07249 mRNA. Translation: AAA18854.1. AL079349 Genomic DNA. Translation: CAB45501.1. AL161535 Genomic DNA. Translation: CAB78344.1. CP002687 Genomic DNA. Translation: AEE83214.1. CP002687 Genomic DNA. Translation: AEE83215.1. AF360324 mRNA. Translation: AAK26034.1. AY056332 mRNA. Translation: AAL07181.1.
IPI	IPI00538802. IPI00547299.
PIR	C85140. S38327.
RefSeq	NP_193038.1. NM_117371.1. NP_849370.1. NM_179039.3.
UniGene	At.236.
3D structure databases
ProteinModelPortal	P43294.
SMR	P43294. Positions 11-309.
ModBase	Search...
Protein-protein interaction databases
IntAct	P43294. 9 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G13020.2; AT4G13020.2; AT4G13020.
GeneID	826915.
KEGG	ath:AT4G13020.
Organism-specific databases
TAIR	At4g13020.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
InParanoid	P43294.
PhylomeDB	P43294.
ProtClustDB	CLSN2685794.
Enzyme and pathway databases
BRENDA	2.7.11.22. 399.
Gene expression databases
ArrayExpress	P43294.
Genevestigator	P43294.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MHK_ARATH

Accession

Primary (citable) accession number: P43294
Secondary accession number(s): Q9C5D4, Q9SV67

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	June 6, 2002
Last modified:	May 29, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program