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P43294

- MHK_ARATH

UniProt

P43294 - MHK_ARATH

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Protein

Serine/threonine-protein kinase MHK

Gene

MHK

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

May play a role in the regulation of plant growth and development.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATPPROSITE-ProRule annotation
Active sitei133 – 1331Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G13020-MONOMER.
ARA:GQT-448-MONOMER.
ARA:GQT-597-MONOMER.
ARA:GQT-598-MONOMER.
ARA:GQT-599-MONOMER.
BRENDAi2.7.11.22. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase MHK (EC:2.7.11.22)
Gene namesi
Name:MHK
Ordered Locus Names:At4g13020
ORF Names:F25G13.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G13020.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443Serine/threonine-protein kinase MHKPRO_0000086325Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei164 – 1641PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Roots, leaves and stems.

Gene expression databases

ExpressionAtlasiP43294. baseline and differential.
GenevestigatoriP43294.

Interactioni

Protein-protein interaction databases

BioGridi12212. 1 interaction.
IntActiP43294. 9 interactions.

Structurei

3D structure databases

ProteinModelPortaliP43294.
SMRiP43294. Positions 9-306.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 291280Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiP43294.
PhylomeDBiP43294.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P43294-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMVFVVFVMC RYKILEELGD GTCGSVYKAV NLETYEVVAV KKMKRKFYYW
60 70 80 90 100
EECVNLREVK ALRKLNHPHI IKLKEIVREH NELFFIFECM DHNLYHIMKE
110 120 130 140 150
RERPFSEGEI RSFMSQMLQG LAHMHKNGYF HRDLKPENLL VTNNILKIAD
160 170 180 190 200
FGLAREVASM PPYTEYVSTR WYRAPEVLLQ SSLYTPAVDM WAVGAILAEL
210 220 230 240 250
YALTPLFPGE SEIDQLYKIC CVLGKPDWTT FPEAKSISRI MSISHTEFPQ
260 270 280 290 300
TRIADLLPNA APEAIDLINR LCSWDPLKRP TADEALNHPF FSMATQASYP
310 320 330 340 350
IHDLELRLDN MAALPNLELN LWDFNREPEE CFLGLTLAVK PSAPKLEMLR
360 370 380 390 400
NVSQDMSENF LFCPGVNNDR EPSVFWSLLS PDENGLHAPV ESSPLSLSFS
410 420 430 440
PMQQHTVGPP QSSGFTMTSS MQPNMLDRPW MAVSAPFQQS HYL
Length:443
Mass (Da):50,896
Last modified:June 6, 2002 - v2
Checksum:iAF92A2233B673F0F
GO
Isoform Short (identifier: P43294-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MMVFVVFVMC → ME

Show »
Length:435
Mass (Da):49,968
Checksum:iE4837811577EB337
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661N → K in AAA18854. (PubMed:8218420)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1010MMVFVVFVMC → ME in isoform Short. CuratedVSP_004861

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07249 mRNA. Translation: AAA18854.1.
AL079349 Genomic DNA. Translation: CAB45501.1.
AL161535 Genomic DNA. Translation: CAB78344.1.
CP002687 Genomic DNA. Translation: AEE83214.1.
CP002687 Genomic DNA. Translation: AEE83215.1.
AF360324 mRNA. Translation: AAK26034.1.
AY056332 mRNA. Translation: AAL07181.1.
PIRiC85140.
S38327.
RefSeqiNP_193038.1. NM_117371.1. [P43294-1]
NP_849370.1. NM_179039.3. [P43294-2]
UniGeneiAt.236.

Genome annotation databases

EnsemblPlantsiAT4G13020.2; AT4G13020.2; AT4G13020. [P43294-1]
GeneIDi826915.
KEGGiath:AT4G13020.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07249 mRNA. Translation: AAA18854.1 .
AL079349 Genomic DNA. Translation: CAB45501.1 .
AL161535 Genomic DNA. Translation: CAB78344.1 .
CP002687 Genomic DNA. Translation: AEE83214.1 .
CP002687 Genomic DNA. Translation: AEE83215.1 .
AF360324 mRNA. Translation: AAK26034.1 .
AY056332 mRNA. Translation: AAL07181.1 .
PIRi C85140.
S38327.
RefSeqi NP_193038.1. NM_117371.1. [P43294-1 ]
NP_849370.1. NM_179039.3. [P43294-2 ]
UniGenei At.236.

3D structure databases

ProteinModelPortali P43294.
SMRi P43294. Positions 9-306.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 12212. 1 interaction.
IntActi P43294. 9 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G13020.2 ; AT4G13020.2 ; AT4G13020 . [P43294-1 ]
GeneIDi 826915.
KEGGi ath:AT4G13020.

Organism-specific databases

TAIRi AT4G13020.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi P43294.
PhylomeDBi P43294.

Enzyme and pathway databases

BioCyci ARA:AT4G13020-MONOMER.
ARA:GQT-448-MONOMER.
ARA:GQT-597-MONOMER.
ARA:GQT-598-MONOMER.
ARA:GQT-599-MONOMER.
BRENDAi 2.7.11.22. 399.

Gene expression databases

ExpressionAtlasi P43294. baseline and differential.
Genevestigatori P43294.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of two novel protein kinase genes from Arabidopsis thaliana."
    Moran T.V., Walker J.C.
    Biochim. Biophys. Acta 1216:9-14(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], ALTERNATIVE SPLICING.
    Strain: cv. Columbia.

Entry informationi

Entry nameiMHK_ARATH
AccessioniPrimary (citable) accession number: P43294
Secondary accession number(s): Q9C5D4, Q9SV67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 6, 2002
Last modified: October 29, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3