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P43294

- MHK_ARATH

UniProt

P43294 - MHK_ARATH

Protein

Serine/threonine-protein kinase MHK

Gene

MHK

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (06 Jun 2002)
      Previous versions | rss
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    Functioni

    May play a role in the regulation of plant growth and development.

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei41 – 411ATPPROSITE-ProRule annotation
    Active sitei133 – 1331Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi18 – 269ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT4G13020-MONOMER.
    ARA:GQT-448-MONOMER.
    ARA:GQT-597-MONOMER.
    ARA:GQT-598-MONOMER.
    ARA:GQT-599-MONOMER.
    BRENDAi2.7.11.22. 399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase MHK (EC:2.7.11.22)
    Gene namesi
    Name:MHK
    Ordered Locus Names:At4g13020
    ORF Names:F25G13.110
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G13020.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 443443Serine/threonine-protein kinase MHKPRO_0000086325Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei164 – 1641PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Tissue specificityi

    Roots, leaves and stems.

    Gene expression databases

    ArrayExpressiP43294.
    GenevestigatoriP43294.

    Interactioni

    Protein-protein interaction databases

    BioGridi12212. 1 interaction.
    IntActiP43294. 9 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP43294.
    SMRiP43294. Positions 11-341.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 291280Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    InParanoidiP43294.
    PhylomeDBiP43294.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P43294-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMVFVVFVMC RYKILEELGD GTCGSVYKAV NLETYEVVAV KKMKRKFYYW    50
    EECVNLREVK ALRKLNHPHI IKLKEIVREH NELFFIFECM DHNLYHIMKE 100
    RERPFSEGEI RSFMSQMLQG LAHMHKNGYF HRDLKPENLL VTNNILKIAD 150
    FGLAREVASM PPYTEYVSTR WYRAPEVLLQ SSLYTPAVDM WAVGAILAEL 200
    YALTPLFPGE SEIDQLYKIC CVLGKPDWTT FPEAKSISRI MSISHTEFPQ 250
    TRIADLLPNA APEAIDLINR LCSWDPLKRP TADEALNHPF FSMATQASYP 300
    IHDLELRLDN MAALPNLELN LWDFNREPEE CFLGLTLAVK PSAPKLEMLR 350
    NVSQDMSENF LFCPGVNNDR EPSVFWSLLS PDENGLHAPV ESSPLSLSFS 400
    PMQQHTVGPP QSSGFTMTSS MQPNMLDRPW MAVSAPFQQS HYL 443
    Length:443
    Mass (Da):50,896
    Last modified:June 6, 2002 - v2
    Checksum:iAF92A2233B673F0F
    GO
    Isoform Short (identifier: P43294-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-10: MMVFVVFVMC → ME

    Show »
    Length:435
    Mass (Da):49,968
    Checksum:iE4837811577EB337
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti66 – 661N → K in AAA18854. (PubMed:8218420)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1010MMVFVVFVMC → ME in isoform Short. CuratedVSP_004861

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07249 mRNA. Translation: AAA18854.1.
    AL079349 Genomic DNA. Translation: CAB45501.1.
    AL161535 Genomic DNA. Translation: CAB78344.1.
    CP002687 Genomic DNA. Translation: AEE83214.1.
    CP002687 Genomic DNA. Translation: AEE83215.1.
    AF360324 mRNA. Translation: AAK26034.1.
    AY056332 mRNA. Translation: AAL07181.1.
    PIRiC85140.
    S38327.
    RefSeqiNP_193038.1. NM_117371.1. [P43294-1]
    NP_849370.1. NM_179039.3. [P43294-2]
    UniGeneiAt.236.

    Genome annotation databases

    EnsemblPlantsiAT4G13020.2; AT4G13020.2; AT4G13020. [P43294-1]
    GeneIDi826915.
    KEGGiath:AT4G13020.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07249 mRNA. Translation: AAA18854.1 .
    AL079349 Genomic DNA. Translation: CAB45501.1 .
    AL161535 Genomic DNA. Translation: CAB78344.1 .
    CP002687 Genomic DNA. Translation: AEE83214.1 .
    CP002687 Genomic DNA. Translation: AEE83215.1 .
    AF360324 mRNA. Translation: AAK26034.1 .
    AY056332 mRNA. Translation: AAL07181.1 .
    PIRi C85140.
    S38327.
    RefSeqi NP_193038.1. NM_117371.1. [P43294-1 ]
    NP_849370.1. NM_179039.3. [P43294-2 ]
    UniGenei At.236.

    3D structure databases

    ProteinModelPortali P43294.
    SMRi P43294. Positions 11-341.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 12212. 1 interaction.
    IntActi P43294. 9 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G13020.2 ; AT4G13020.2 ; AT4G13020 . [P43294-1 ]
    GeneIDi 826915.
    KEGGi ath:AT4G13020.

    Organism-specific databases

    TAIRi AT4G13020.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    InParanoidi P43294.
    PhylomeDBi P43294.

    Enzyme and pathway databases

    BioCyci ARA:AT4G13020-MONOMER.
    ARA:GQT-448-MONOMER.
    ARA:GQT-597-MONOMER.
    ARA:GQT-598-MONOMER.
    ARA:GQT-599-MONOMER.
    BRENDAi 2.7.11.22. 399.

    Gene expression databases

    ArrayExpressi P43294.
    Genevestigatori P43294.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of two novel protein kinase genes from Arabidopsis thaliana."
      Moran T.V., Walker J.C.
      Biochim. Biophys. Acta 1216:9-14(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], ALTERNATIVE SPLICING.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiMHK_ARATH
    AccessioniPrimary (citable) accession number: P43294
    Secondary accession number(s): Q9C5D4, Q9SV67
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: June 6, 2002
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3