Reviewed, UniProtKB/Swiss-Prot P43292 (SRK2G_ARATH)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase SRK2G
    EC=2.7.11.1
Alternative name(s):
    Arabidopsis protein SK1
    OST1-kinase-like 8
    SNF1-related kinase 2.1
      Short name=SnRK2.1

Gene names

Name:	SRK2G
Synonyms:	ASK2, OSKL8, SNRK2.1
Ordered Locus Names:	At5g08590
ORF Names:	MAH20.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Nucleus.
Tissue specificity	Expressed in seedlings.
Induction	By abscisic acid (ABA), salt, and osmotic stress.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ADO3	Q9C9W9	2	EBI-401174,EBI-401228

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	Serine/threonine-protein kinase SRK2G		PRO_0000085637
Regions
	Domain	4 – 260	257	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
	Compositional bias	319 – 343	25	Asp/Glu-rich (acidic)
Sites
	Active site	123	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Amino acid modifications
	Modified residue	149	1	Phosphoserine
	Modified residue	150	1	Phosphoserine
	Modified residue	154	1	Phosphoserine
	Modified residue	158	1	Phosphoserine
	Modified residue	159	1	Phosphothreonine
Experimental info
	Sequence conflict	55	1	L → F in CAA78106. Ref.1
	Sequence conflict	134	1	S → A in CAA78106. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P43292-1 [UniParc]. Last modified December 6, 2002. Version 2. Checksum: 7176CE43659FCCA4	FASTA	353	40,198
	10 20 30 40 50 60 MDKYDVVKDL GAGNFGVARL LRHKDTKELV AMKYIERGRK IDENVAREII NHRSLKHPNI 70 80 90 100 110 120 IRFKEVILTP THLAIVMEYA SGGELFDRIC TAGRFSEAEA RYFFQQLICG VDYCHSLQIC 130 140 150 160 170 180 HRDLKLENTL LDGSPAPLLK ICDFGYSKSS ILHSRPKSTV GTPAYIAPEV LSRREYDGKH 190 200 210 220 230 240 ADVWSCGVTL YVMLVGAYPF EDPNDPKNFR KTIQRIMAVQ YKIPDYVHIS QECKHLLSRI 250 260 270 280 290 300 FVTNSAKRIT LKEIKNHPWY LKNLPKELLE SAQAAYYKRD TSFSLQSVED IMKIVGEARN 310 320 330 340 350 PAPSTSAVKS SGSGADEEEE EDVEAEVEEE EDDEDEYEKH VKEAQSCQES DKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two putative protein kinases from Arabidopsis thaliana contain highly acidic domains." Park Y.S., Hong S.W., Oh S.A., Kwak J.M., Lee H.H., Nam H.G. Plant Mol. Biol. 22:615-624(1993) [PubMed: 8393717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones." Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:291-300(1997) [PubMed: 9405937] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Shinn P., Chen H., Kim C.J., Ecker J.R. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Site-specific phosphorylation profiling of Arabidopsis proteins by mass spectrometry and peptide chip analysis." de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E., Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J., Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H. J. Proteome Res. 7:2458-2470(2008) [PubMed: 18433157] [Abstract] Cited for: PROTEIN SEQUENCE OF 148-174, PHOSPHORYLATION, SUBCELLULAR LOCALIZATION, IDENTIFICATION BY MASS-SPECTROMETRY.
[5]	"The Arabidopsis CDPK-SnRK superfamily of protein kinases." Hrabak E.M., Chan C.W.M., Gribskov M., Harper J.F., Choi J.H., Halford N., Kudla J., Luan S., Nimmo H.G., Sussman M.R., Thomas M., Walker-Simmons K., Zhu J.-K., Harmon A.C. Plant Physiol. 132:666-680(2003) [PubMed: 12805596] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Identification of nine sucrose nonfermenting 1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana." Boudsocq M., Barbier-Brygoo H., Lauriere C. J. Biol. Chem. 279:41758-41766(2004) [PubMed: 15292193] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[7]	"The regulatory domain of SRK2E/OST1/SnRK2.6 interacts with ABI1 and integrates abscisic acid (ABA) and osmotic stress signals controlling stomatal closure in Arabidopsis." Yoshida R., Umezawa T., Mizoguchi T., Takahashi S., Takahashi F., Shinozaki K. J. Biol. Chem. 281:5310-5318(2006) [PubMed: 16365038] [Abstract] Cited for: GENE FAMILY, INDUCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z12120 mRNA. Translation: CAA78106.1. AB006697 Genomic DNA. Translation: BAB10008.1. BT024886 mRNA. Translation: ABD85157.1.
PIR	S24586.
RefSeq	NP_196476.1.
UniGene	At.23718
3D structure databases
HSSP	HSSP built from PDB template 1IA8 based on UniProtKB O14757.
ModBase	Search...
Protein-protein interaction databases
IntAct	P43292.
Genome annotation databases
GeneID	830760.
GenomeReviews	Gene locus AT5G08590 in contig BA000015_GR.
KEGG	ath:AT5G08590.
NMPDR	fig|3702.1.peg.23012.
Organism-specific databases
TAIR	At5g08590.
Gene expression databases
ArrayExpress	P43292.
GermOnline	AT5G08590. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR015740. S/T_plantPK. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22982:SF58. S/T_plantPK. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SRK2G_ARATH

Accession

Primary (citable) accession number: P43292
Secondary accession number(s): Q24JK3, Q9FNM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	December 6, 2002
Last modified:	November 25, 2008
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)