Reviewed, UniProtKB/Swiss-Prot P43291 (SRK2A_ARATH)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase SRK2A
    EC=2.7.11.1
Alternative name(s):
    Arabidopsis protein SK1
    OST1-kinase-like 7
    SNF1-related kinase 2.4
      Short name=SnRK2.4

Gene names

Name:	SRK2A
Synonyms:	ASK1, OSKL7, SNRK2.4
Ordered Locus Names:	At1g10940
ORF Names:	T19D16.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Expressed in seedlings.
Induction	By abscisic acid (ABA), salt, and osmotic stress (at protein level).
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ADO3	Q9C9W9	2	EBI-401164,EBI-401228
EBF1	Q9SKK0	1	EBI-401164,EBI-401198
EBF2	Q708Y0	1	EBI-401164,EBI-593623

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Serine/threonine-protein kinase SRK2A		PRO_0000085636
Regions
	Domain	4 – 260	257	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
	Compositional bias	319 – 348	30	Asp/Glu-rich (acidic)
Sites
	Active site	123	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Amino acid modifications
	Modified residue	149	1	Phosphoserine By similarity
	Modified residue	150	1	Phosphoserine By similarity
	Modified residue	154	1	Phosphoserine By similarity
	Modified residue	158	1	Phosphoserine By similarity
	Modified residue	159	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43291-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 89C31C87AB6C6E78	FASTA	363	41,169
	10 20 30 40 50 60 MDKYELVKDI GAGNFGVARL MKVKNSKELV AMKYIERGPK IDENVAREII NHRSLRHPNI 70 80 90 100 110 120 IRFKEVVLTP THLAIAMEYA AGGELFERIC SAGRFSEDEA RYFFQQLISG VSYCHAMQIC 130 140 150 160 170 180 HRDLKLENTL LDGSPAPRLK ICDFGYSKSS LLHSRPKSTV GTPAYIAPEV LSRREYDGKM 190 200 210 220 230 240 ADVWSCGVTL YVMLVGAYPF EDQEDPKNFR KTIQKIMAVQ YKIPDYVHIS QDCKNLLSRI 250 260 270 280 290 300 FVANSLKRIT IAEIKKHSWF LKNLPRELTE TAQAAYFKKE NPTFSLQTVE EIMKIVADAK 310 320 330 340 350 360 TPPPVSRSIG GFGWGGNGDA DGKEEDAEDV EEEEEEVEEE EDDEDEYDKT VKEVHASGEV RIS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two putative protein kinases from Arabidopsis thaliana contain highly acidic domains." Park Y.S., Hong S.W., Oh S.A., Kwak J.M., Lee H.H., Nam H.G. Plant Mol. Biol. 22:615-624(1993) [PubMed: 8393717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The Arabidopsis CDPK-SnRK superfamily of protein kinases." Hrabak E.M., Chan C.W.M., Gribskov M., Harper J.F., Choi J.H., Halford N., Kudla J., Luan S., Nimmo H.G., Sussman M.R., Thomas M., Walker-Simmons K., Zhu J.-K., Harmon A.C. Plant Physiol. 132:666-680(2003) [PubMed: 12805596] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Identification of nine sucrose nonfermenting 1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana." Boudsocq M., Barbier-Brygoo H., Lauriere C. J. Biol. Chem. 279:41758-41766(2004) [PubMed: 15292193] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[7]	"The regulatory domain of SRK2E/OST1/SnRK2.6 interacts with ABI1 and integrates abscisic acid (ABA) and osmotic stress signals controlling stomatal closure in Arabidopsis." Yoshida R., Umezawa T., Mizoguchi T., Takahashi S., Takahashi F., Shinozaki K. J. Biol. Chem. 281:5310-5318(2006) [PubMed: 16365038] [Abstract] Cited for: GENE FAMILY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M91548 mRNA. Translation: AAA02840.1. U95973 Genomic DNA. Translation: AAB65483.1. AY093130 mRNA. Translation: AAM13129.1. BT008850 mRNA. Translation: AAP68289.1. AY084221 mRNA. Translation: AAM60822.1.
PIR	S36944.
RefSeq	NP_172563.1.
UniGene	At.23750
3D structure databases
HSSP	HSSP built from PDB template 1GZK based on UniProtKB P31751.
ModBase	Search...
Protein-protein interaction databases
IntAct	P43291.
Proteomic databases
ProMEX	P43291.
Genome annotation databases
GeneID	837637.
GenomeReviews	Gene locus AT1G10940 in contig CT485782_GR.
KEGG	ath:AT1G10940.
NMPDR	fig|3702.1.peg.1343.
Organism-specific databases
TAIR	At1g10940.
Gene expression databases
ArrayExpress	P43291.
GermOnline	AT1G10940. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR015740. S/T_plantPK. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22982:SF58. S/T_plantPK. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SRK2A_ARATH

Accession

Primary (citable) accession number: P43291

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 25, 2008
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)