P43291 (SRK2A_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 104.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Serine/threonine-protein kinase SRK2A EC=2.7.11.1 Alternative name(s): Arabidopsis protein SK1 OST1-kinase-like 7 SNF1-related kinase 2.4 Short name=SnRK2.4 | ||||||||
| Gene names |
| ||||||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 363 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Tissue specificity | Expressed in seedlings. Ref.7 |
| Induction | By abscisic acid (ABA), salt, and osmotic stress (at protein level). Ref.7 |
| Sequence similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | response to abscisic acid stimulus Inferred from direct assay. Source: TAIR response to salt stressInferred from direct assay Ref.7. Source: TAIR |
| Cellular component | cytosol Inferred from direct assay. Source: TAIR nucleusInferred from direct assay. Source: TAIR |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein bindingInferred from physical interaction. Source: IntAct protein serine/threonine kinase activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| EBF1 | Q9SKK0 | 2 | EBI-401164,EBI-401198 | |
| EBF2 | Q708Y0 | 2 | EBI-401164,EBI-593623 | |
| FBL17 | Q8W104 | 2 | EBI-401164,EBI-2026732 | |
| UFO | Q39090 | 3 | EBI-401164,EBI-590758 |
Alternative products
| This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences. | ||||||
| Isoform 1 (identifier: P43291-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 363 | 363 | Serine/threonine-protein kinase SRK2A | PRO_0000085636 | |||||
Regions | |||||||||
| Domain | 4 – 260 | 257 | Protein kinase | ||||||
| Nucleotide binding | 10 – 18 | 9 | ATP By similarity | ||||||
| Compositional bias | 319 – 348 | 30 | Asp/Glu-rich (acidic) | ||||||
Sites | |||||||||
| Active site | 123 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 33 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 149 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 150 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 154 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 158 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 159 | 1 | Phosphothreonine By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Two putative protein kinases from Arabidopsis thaliana contain highly acidic domains." Park Y.S., Hong S.W., Oh S.A., Kwak J.M., Lee H.H., Nam H.G. Plant Mol. Biol. 22:615-624(1993) [PubMed: 8393717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf. |
| [2] | "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W.Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [4] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [5] | "Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [6] | "The Arabidopsis CDPK-SnRK superfamily of protein kinases." Hrabak E.M., Chan C.W.M., Gribskov M., Harper J.F., Choi J.H., Halford N., Kudla J., Luan S., Nimmo H.G., Sussman M.R., Thomas M., Walker-Simmons K., Zhu J.-K., Harmon A.C. Plant Physiol. 132:666-680(2003) [PubMed: 12805596] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE. |
| [7] | "Identification of nine sucrose nonfermenting 1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana." Boudsocq M., Barbier-Brygoo H., Lauriere C. J. Biol. Chem. 279:41758-41766(2004) [PubMed: 15292193] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION. |
| [8] | "The regulatory domain of SRK2E/OST1/SnRK2.6 interacts with ABI1 and integrates abscisic acid (ABA) and osmotic stress signals controlling stomatal closure in Arabidopsis." Yoshida R., Umezawa T., Mizoguchi T., Takahashi S., Takahashi F., Shinozaki K. J. Biol. Chem. 281:5310-5318(2006) [PubMed: 16365038] [Abstract] Cited for: GENE FAMILY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M91548 mRNA. Translation: AAA02840.1. U95973 Genomic DNA. Translation: AAB65483.1. CP002684 Genomic DNA. Translation: AEE28666.1. AY093130 mRNA. Translation: AAM13129.1. BT008850 mRNA. Translation: AAP68289.1. AY084221 mRNA. Translation: AAM60822.1. |
| IPI | IPI00522193. |
| PIR | S36944. |
| RefSeq | NP_172563.1. NM_100969.3. |
| UniGene | At.23750. |
3D structure databases | |
| ProteinModelPortal | P43291. |
| SMR | P43291. Positions 1-284. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P43291. 14 interactions. |
| STRING | P43291. |
Proteomic databases | |
| PRIDE | P43291. |
| ProMEX | P43291. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT1G10940.1; AT1G10940.1; AT1G10940. |
| GeneID | 837637. |
| GenomeReviews | Gene locus AT1G10940 in contig CT485782_GR. |
| KEGG | ath:AT1G10940. |
| NMPDR | fig|3702.1.peg.1343. |
Organism-specific databases | |
| TAIR | At1g10940. |
Phylogenomic databases | |
| eggNOG | KOG0583. |
| HOGENOM | HBG755340. |
| InParanoid | P43291. |
| OMA | LAIAMEY. |
| PhylomeDB | P43291. |
| ProtClustDB | CLSN2681826. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.1. 399. |
Gene expression databases | |
| ArrayExpress | P43291. |
| Genevestigator | P43291. |
| GermOnline | AT1G10940. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view] |
| KO | K14498. |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| SUPFAM | SSF56112. Kinase_like. 1 hit. |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | SRK2A_ARATH | ||||||||
| Accession | Primary (citable) accession number: P43291 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with