Serine/threonine-protein kinase SRK2A - Arabidopsis thaliana (Mouse-ear cress)

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P43291 (SRK2A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase SRK2A
EC=2.7.11.1

Alternative name(s):
Arabidopsis protein SK1
OST1-kinase-like 7
SNF1-related kinase 2.4
Short name=SnRK2.4

Gene names

Name:	SRK2A
Synonyms:	ASK1, OSKL7, SNRK2.4
Ordered Locus Names:	At1g10940
ORF Names:	T19D16.14

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Expressed in seedlings. Ref.7
Induction	By abscisic acid (ABA), salt, and osmotic stress (at protein level). Ref.7
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to abscisic acid stimulus Inferred from direct assay. Source: TAIR response to salt stress Inferred from direct assay Ref.7. Source: TAIR
Cellular component	cytosol Inferred from direct assay. Source: TAIR nucleus Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
EBF1	Q9SKK0	2	EBI-401164,EBI-401198
EBF2	Q708Y0	2	EBI-401164,EBI-593623
FBL17	Q8W104	2	EBI-401164,EBI-2026732
UFO	Q39090	3	EBI-401164,EBI-590758

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P43291-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 363

363

Serine/threonine-protein kinase SRK2A

PRO_0000085636

Regions

Domain

4 – 260

257

Protein kinase

Nucleotide binding

10 – 18

ATP By similarity

Compositional bias

319 – 348

Asp/Glu-rich (acidic)

Sites

Active site

123

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

149

Phosphoserine By similarity

Modified residue

150

Phosphoserine By similarity

Modified residue

154

Phosphoserine By similarity

Modified residue

158

Phosphoserine By similarity

Modified residue

159

Phosphothreonine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 89C31C87AB6C6E78
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FASTA

363

41,169

        10         20         30         40         50         60 
MDKYELVKDI GAGNFGVARL MKVKNSKELV AMKYIERGPK IDENVAREII NHRSLRHPNI 

        70         80         90        100        110        120 
IRFKEVVLTP THLAIAMEYA AGGELFERIC SAGRFSEDEA RYFFQQLISG VSYCHAMQIC 

       130        140        150        160        170        180 
HRDLKLENTL LDGSPAPRLK ICDFGYSKSS LLHSRPKSTV GTPAYIAPEV LSRREYDGKM 

       190        200        210        220        230        240 
ADVWSCGVTL YVMLVGAYPF EDQEDPKNFR KTIQKIMAVQ YKIPDYVHIS QDCKNLLSRI 

       250        260        270        280        290        300 
FVANSLKRIT IAEIKKHSWF LKNLPRELTE TAQAAYFKKE NPTFSLQTVE EIMKIVADAK 

       310        320        330        340        350        360 
TPPPVSRSIG GFGWGGNGDA DGKEEDAEDV EEEEEEVEEE EDDEDEYDKT VKEVHASGEV 


RIS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two putative protein kinases from Arabidopsis thaliana contain highly acidic domains." Park Y.S., Hong S.W., Oh S.A., Kwak J.M., Lee H.H., Nam H.G. Plant Mol. Biol. 22:615-624(1993) [PubMed: 8393717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The Arabidopsis CDPK-SnRK superfamily of protein kinases." Hrabak E.M., Chan C.W.M., Gribskov M., Harper J.F., Choi J.H., Halford N., Kudla J., Luan S., Nimmo H.G., Sussman M.R., Thomas M., Walker-Simmons K., Zhu J.-K., Harmon A.C. Plant Physiol. 132:666-680(2003) [PubMed: 12805596] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[7]	"Identification of nine sucrose nonfermenting 1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana." Boudsocq M., Barbier-Brygoo H., Lauriere C. J. Biol. Chem. 279:41758-41766(2004) [PubMed: 15292193] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[8]	"The regulatory domain of SRK2E/OST1/SnRK2.6 interacts with ABI1 and integrates abscisic acid (ABA) and osmotic stress signals controlling stomatal closure in Arabidopsis." Yoshida R., Umezawa T., Mizoguchi T., Takahashi S., Takahashi F., Shinozaki K. J. Biol. Chem. 281:5310-5318(2006) [PubMed: 16365038] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M91548 mRNA. Translation: AAA02840.1. U95973 Genomic DNA. Translation: AAB65483.1. CP002684 Genomic DNA. Translation: AEE28666.1. AY093130 mRNA. Translation: AAM13129.1. BT008850 mRNA. Translation: AAP68289.1. AY084221 mRNA. Translation: AAM60822.1.
IPI	IPI00522193.
PIR	S36944.
RefSeq	NP_172563.1. NM_100969.3.
UniGene	At.23750.
3D structure databases
ProteinModelPortal	P43291.
SMR	P43291. Positions 1-284.
ModBase	Search...
Protein-protein interaction databases
IntAct	P43291. 14 interactions.
STRING	P43291.
Proteomic databases
PRIDE	P43291.
ProMEX	P43291.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G10940.1; AT1G10940.1; AT1G10940.
GeneID	837637.
GenomeReviews	Gene locus AT1G10940 in contig CT485782_GR.
KEGG	ath:AT1G10940.
NMPDR	fig\|3702.1.peg.1343.
Organism-specific databases
TAIR	At1g10940.
Phylogenomic databases
eggNOG	KOG0583.
HOGENOM	HBG755340.
InParanoid	P43291.
OMA	LAIAMEY.
PhylomeDB	P43291.
ProtClustDB	CLSN2681826.
Enzyme and pathway databases
BRENDA	2.7.11.1. 399.
Gene expression databases
ArrayExpress	P43291.
Genevestigator	P43291.
GermOnline	AT1G10940. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K14498.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SRK2A_ARATH

Accession

Primary (citable) accession number: P43291

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents