P43280 (METK1_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-adenosylmethionine synthase 1
Short name=AdoMet synthase 1
EC=2.5.1.6

Alternative name(s):
Methionine adenosyltransferase 1
Short name=MAT 1

Gene names

Name:

SAM1

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Mostly expressed in stems. Ref.1
Induction	By salt stress, ABA, and mannitol in roots and leaves. Ref.1
Sequence similarities	Belongs to the AdoMet synthase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	S-adenosylmethionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	S-adenosylmethionine synthase 1		PRO_0000174464
Regions
	Nucleotide binding	119 – 124	6	ATP Potential
	Nucleotide binding	267 – 274	8	ATP Potential
Sites
	Metal binding	17	1	Magnesium By similarity
	Metal binding	43	1	Potassium By similarity
	Metal binding	271	1	Potassium By similarity
	Metal binding	279	1	Magnesium By similarity
	Binding site	147	1	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P43280 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 81433A565A68039B	FASTA	393	43,301
	10 20 30 40 50 60 METFLFTSES VNEGHPDKLC DQISDAVLDA CLEQDPESKV ACETCTKTNL VMVFGEITTK 70 80 90 100 110 120 AIVDYEKIVR DTCRNIGFVS DDVGLDADNC KVLVYIEQQS PDIAQGVHGH LTKRPEEIGA 130 140 150 160 170 180 GDQGHMFGYA TDETPELMPL SHVLATKLGA RLTEVRKNGT CAWLRPDGKT QVTVEYSNDN 190 200 210 220 230 240 GAMVPIRVHT VLISTQHDET VTNDEIARDL KEHVIKPVIP EKYLDENTIF HLNPSGRFVI 250 260 270 280 290 300 GGPHGDAGLT GRKIIIDTYG GWGAHGGGAF SGKDPTKVDR SGAYIVRQAA KSIVASGLAR 310 320 330 340 350 360 RCIVQVSYAI GVPEPLSVFV DTYGTGKIPD REILKIVKEN FDFRPGMMSI NLDLKRGGNR 370 380 390 RFLKTAAYGH FGRDDPDFTW EVVKPLKWEK PQD

References

[1]

"Differential accumulation of S-adenosylmethionine synthetase transcripts in response to salt stress." Espartero J., Pintor-Toro J.A., Pardo J.M. Plant Mol. Biol. 25:217-227(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Rutgers. Tissue: Seedling.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z24741 mRNA. Translation: CAA80865.1.
PIR	S46538.
RefSeq	NP_001234425.1. NM_001247496.1.
UniGene	Les.226.
3D structure databases
ProteinModelPortal	P43280.
SMR	P43280. Positions 3-388.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	Solyc01g101060.2.1; Solyc01g101060.2.1; Solyc01g101060.2.
GeneID	544155.
Enzyme and pathway databases
UniPathway	UPA00315; UER00080.
Family and domain databases
InterPro	IPR022631. ADOMET_SYNTHASE_CS. IPR022630. S-AdoMet_synt_C. IPR022629. S-AdoMet_synt_central. IPR022628. S-AdoMet_synt_N. IPR002133. S-AdoMet_synthetase. IPR022636. S-AdoMet_synthetase_sfam. [Graphical view]
PANTHER	PTHR11964. PTHR11964. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
SUPFAM	SSF55973. S-AdoMet_synt. 3 hits.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHASE_1. 1 hit. PS00377. ADOMET_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK1_SOLLC

Accession

Primary (citable) accession number: P43280

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program