P43280 (METK1_SOLLC) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 85.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: S-adenosylmethionine synthase 1 Short name=AdoMet synthase 1 EC=2.5.1.6 Alternative name(s): Methionine adenosyltransferase 1 Short name=MAT 1 | ||
| Gene names |
| ||
| Organism | Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome] | ||
| Taxonomic identifier | 4081 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon![]() |
Protein attributes
| Sequence length | 393 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity. |
| Catalytic activity | ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine. |
| Cofactor | Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity. |
| Pathway | |
| Subunit structure | Homotetramer By similarity. |
| Subcellular location | Cytoplasm By similarity. |
| Tissue specificity | Mostly expressed in stems. Ref.1 |
| Induction | By salt stress, ABA, and mannitol in roots and leaves. Ref.1 |
| Sequence similarities | Belongs to the AdoMet synthase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | One-carbon metabolism |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium |
| Molecular function | Transferase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | S-adenosylmethionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway one-carbon metabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular_component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 393 | 393 | S-adenosylmethionine synthase 1 | PRO_0000174464 | |||||
Regions | |||||||||
| Nucleotide binding | 119 – 124 | 6 | ATP Potential | ||||||
| Nucleotide binding | 267 – 274 | 8 | ATP Potential | ||||||
Sites | |||||||||
| Metal binding | 17 | 1 | Magnesium By similarity | ||||||
| Metal binding | 43 | 1 | Potassium By similarity | ||||||
| Metal binding | 271 | 1 | Potassium By similarity | ||||||
| Metal binding | 279 | 1 | Magnesium By similarity | ||||||
| Binding site | 147 | 1 | ATP Potential | ||||||
Sequences
| ||||||||||||||||||
References
| [1] | "Differential accumulation of S-adenosylmethionine synthetase transcripts in response to salt stress." Espartero J., Pintor-Toro J.A., Pardo J.M. Plant Mol. Biol. 25:217-227(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Rutgers. Tissue: Seedling. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Z24741 mRNA. Translation: CAA80865.1. |
| PIR | S46538. |
| RefSeq | NP_001234425.1. NM_001247496.1. |
| UniGene | Les.226. |
3D structure databases | |
| ProteinModelPortal | P43280. |
| SMR | P43280. Positions 3-388. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | Solyc01g101060.2.1; Solyc01g101060.2.1; Solyc01g101060.2. |
| GeneID | 544155. |
Enzyme and pathway databases | |
| UniPathway | UPA00315; UER00080. |
Family and domain databases | |
| InterPro | IPR022631. ADOMET_SYNTHASE_CS. IPR022630. S-AdoMet_synt_C. IPR022629. S-AdoMet_synt_central. IPR022628. S-AdoMet_synt_N. IPR002133. S-AdoMet_synthetase. IPR022636. S-AdoMet_synthetase_sfam. [Graphical view] |
| PANTHER | PTHR11964. PTHR11964. 1 hit. |
| Pfam | PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF000497. MAT. 1 hit. |
| SUPFAM | SSF55973. S-AdoMet_synt. 3 hits. |
| TIGRFAMs | TIGR01034. metK. 1 hit. |
| PROSITE | PS00376. ADOMET_SYNTHASE_1. 1 hit. PS00377. ADOMET_SYNTHASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | METK1_SOLLC | ||||||||
| Accession | Primary (citable) accession number: P43280 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with
