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P43279

- MAOC_ORYSJ

UniProt

P43279 - MAOC_ORYSJ

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Protein
NADP-dependent malic enzyme, chloroplastic
Gene
ME6, Os01g0188400, LOC_Os01g09320, P0512G09.20, P0695A04.29
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

The chloroplastic ME isoform decarboxylates malate shuttled from neighboring mesophyll cells. The CO2 released is then refixed by ribulose-bisphosphate carboxylase. This pathway eliminates the photorespiratory loss of CO2 that occurs in most plants.

Catalytic activityi

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Divalent metal cations. Prefers magnesium or manganese By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei187 – 1871Proton donor By similarity
Binding sitei240 – 2401NAD By similarity
Active sitei258 – 2581Proton acceptor By similarity
Metal bindingi330 – 3301Divalent metal cation By similarity
Metal bindingi331 – 3311Divalent metal cation By similarity
Metal bindingi354 – 3541Divalent metal cation By similarity
Binding sitei354 – 3541NAD By similarity
Sitei354 – 3541Important for activity By similarity
Binding sitei495 – 4951NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi383 – 39917NADP By similarity
Add
BLAST

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. malate dehydrogenase (decarboxylating) (NAD+) activity Source: InterPro
  3. malate dehydrogenase (decarboxylating) (NADP+) activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW
  5. oxaloacetate decarboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. malate metabolic process Source: InterPro
  2. protein homooligomerization Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, NADP

Enzyme and pathway databases

ReactomeiREACT_206405. PPARA activates gene expression.
UniPathwayiUPA00322.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-dependent malic enzyme, chloroplastic (EC:1.1.1.40)
Short name:
NADP-ME
Gene namesi
Name:ME6
Ordered Locus Names:Os01g0188400, LOC_Os01g09320
ORF Names:P0512G09.20, P0695A04.29
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 1

Organism-specific databases

GrameneiP43279.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
  2. cytosol Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4949Chloroplast Reviewed prediction
Add
BLAST
Chaini50 – 639590NADP-dependent malic enzyme, chloroplastic
PRO_0000018548Add
BLAST

Proteomic databases

PRIDEiP43279.

Interactioni

Subunit structurei

Homotetramer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP43279.
SMRiP43279. Positions 99-639.

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0281.
KOiK00029.
OMAiEEAYTHT.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43279-1 [UniParc]FASTAAdd to Basket

« Hide

MLSARAAATA AAAAASPLWK RGEGGSSGSG SGCTSCREVR RRAAAVRVRA    50
AAPRRVEAVA MESAAETEKK EEVAAAGGGV EDMATEEVPV TPWAFSVASG 100
YTLLRDPHHN KGLAFSEKER DAHYLRGLLP PAVVSQDLQV KKIMHNLRQY 150
SVPLQRYMAM MDLQERNERL FYKLLIDNVE ELLPVVYTPT VGEACQKYGS 200
IFRQPQGLYV SLKDKGKVLD VLRNWPERNI QVIVVTDGER ILGLGDLGCQ 250
GMGIPVGKLS LYTALGGVRP SACLPITIDV GTNNEQLLND EFYIGLRQRR 300
ATGKEYHELM EEFMSAVKQI YGEKVLIQFE DFANHNAFDL LAKYSKSHLV 350
FNDDIQGTAS VVLAGLLSSL KVVGGTLAEH TYLFLGAGEA GTGIAELIAL 400
EISKQTKAPI EECRKKVWLL DSKGLIVNSR KESLQAFKKP WAHEHEPVTT 450
LLDAVQSIKP TVLIGTSGVG KTFTKEVIEA MASFNERPVI FSLSNPTSHS 500
ECTAEEAYNW SQGRAVFASG SPFDPVEYNG KIHVPGQSNN AYIFPGFGLG 550
VVISGAVRVH EDMLLAASET LADQATQENF EKGSIFPPFT NIRKISARIA 600
ASVAAKAYEL GLATRLPQPR DLEKYAESCM YTPVYRSYR 639
Length:639
Mass (Da):69,866
Last modified:May 30, 2006 - v2
Checksum:iA12C763D31D8D25D
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451Missing in BAA03949. 1 Publication
Sequence conflicti50 – 523AAA → TPV in BAA03949. 1 Publication
Sequence conflicti494 – 4941S → A in BAA03949. 1 Publication
Sequence conflicti602 – 6021S → T in BAA03949. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16499 mRNA. Translation: BAA03949.1.
AP002816 Genomic DNA. Translation: BAB03427.1.
AP002836 Genomic DNA. Translation: BAB07934.1.
PIRiS46499.
RefSeqiNP_001042257.1. NM_001048792.1.
UniGeneiOs.12492.

Genome annotation databases

EnsemblPlantsiOS01T0188400-01; OS01T0188400-01; OS01G0188400.
GeneIDi4326769.
KEGGiosa:4326769.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16499 mRNA. Translation: BAA03949.1 .
AP002816 Genomic DNA. Translation: BAB03427.1 .
AP002836 Genomic DNA. Translation: BAB07934.1 .
PIRi S46499.
RefSeqi NP_001042257.1. NM_001048792.1.
UniGenei Os.12492.

3D structure databases

ProteinModelPortali P43279.
SMRi P43279. Positions 99-639.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P43279.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS01T0188400-01 ; OS01T0188400-01 ; OS01G0188400 .
GeneIDi 4326769.
KEGGi osa:4326769.

Organism-specific databases

Gramenei P43279.

Phylogenomic databases

eggNOGi COG0281.
KOi K00029.
OMAi EEAYTHT.

Enzyme and pathway databases

UniPathwayi UPA00322 .
Reactomei REACT_206405. PPARA activates gene expression.

Family and domain databases

Gene3Di 3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view ]
PIRSFi PIRSF000106. ME. 1 hit.
PRINTSi PR00072. MALOXRDTASE.
SMARTi SM00919. Malic_M. 1 hit.
[Graphical view ]
PROSITEi PS00331. MALIC_ENZYMES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a rice cDNA similar to a maize NADP-dependent malic enzyme."
    Fushimi T., Umeda M., Shimazaki T., Kato A., Toriyama K., Uchimiya H.
    Plant Mol. Biol. 24:965-967(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Callus.
  2. "The genome sequence and structure of rice chromosome 1."
    Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
    , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
    Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiMAOC_ORYSJ
AccessioniPrimary (citable) accession number: P43279
Secondary accession number(s): Q9LDH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 30, 2006
Last modified: September 3, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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