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Protein

NADP-dependent malic enzyme, chloroplastic

Gene

ME6

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The chloroplastic ME isoform decarboxylates malate shuttled from neighboring mesophyll cells. The CO2 released is then refixed by ribulose-bisphosphate carboxylase. This pathway eliminates the photorespiratory loss of CO2 that occurs in most plants.

Catalytic activityi

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity

Pathway: C4 acid pathway

This protein is involved in the pathway C4 acid pathway, which is part of Photosynthesis.
View all proteins of this organism that are known to be involved in the pathway C4 acid pathway and in Photosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei187 – 1871Proton donorBy similarity
Binding sitei240 – 2401NADBy similarity
Active sitei258 – 2581Proton acceptorBy similarity
Metal bindingi330 – 3301Divalent metal cationBy similarity
Metal bindingi331 – 3311Divalent metal cationBy similarity
Metal bindingi354 – 3541Divalent metal cationBy similarity
Binding sitei354 – 3541NADBy similarity
Sitei354 – 3541Important for activityBy similarity
Binding sitei495 – 4951NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi383 – 39917NADPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00322.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-dependent malic enzyme, chloroplastic (EC:1.1.1.40)
Short name:
NADP-ME
Gene namesi
Name:ME6
Ordered Locus Names:Os01g0188400, LOC_Os01g09320
ORF Names:P0512G09.20, P0695A04.29
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
ProteomesiUP000000763 Componenti: Chromosome 1

Organism-specific databases

GrameneiP43279.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4949ChloroplastSequence AnalysisAdd
BLAST
Chaini50 – 639590NADP-dependent malic enzyme, chloroplasticPRO_0000018548Add
BLAST

Proteomic databases

PRIDEiP43279.

Expressioni

Gene expression databases

ExpressionAtlasiP43279. baseline.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os01g09320.1.

Structurei

3D structure databases

ProteinModelPortaliP43279.
SMRiP43279. Positions 99-639.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0281.
InParanoidiP43279.
KOiK00029.
OMAiEEAYNWT.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43279-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSARAAATA AAAAASPLWK RGEGGSSGSG SGCTSCREVR RRAAAVRVRA
60 70 80 90 100
AAPRRVEAVA MESAAETEKK EEVAAAGGGV EDMATEEVPV TPWAFSVASG
110 120 130 140 150
YTLLRDPHHN KGLAFSEKER DAHYLRGLLP PAVVSQDLQV KKIMHNLRQY
160 170 180 190 200
SVPLQRYMAM MDLQERNERL FYKLLIDNVE ELLPVVYTPT VGEACQKYGS
210 220 230 240 250
IFRQPQGLYV SLKDKGKVLD VLRNWPERNI QVIVVTDGER ILGLGDLGCQ
260 270 280 290 300
GMGIPVGKLS LYTALGGVRP SACLPITIDV GTNNEQLLND EFYIGLRQRR
310 320 330 340 350
ATGKEYHELM EEFMSAVKQI YGEKVLIQFE DFANHNAFDL LAKYSKSHLV
360 370 380 390 400
FNDDIQGTAS VVLAGLLSSL KVVGGTLAEH TYLFLGAGEA GTGIAELIAL
410 420 430 440 450
EISKQTKAPI EECRKKVWLL DSKGLIVNSR KESLQAFKKP WAHEHEPVTT
460 470 480 490 500
LLDAVQSIKP TVLIGTSGVG KTFTKEVIEA MASFNERPVI FSLSNPTSHS
510 520 530 540 550
ECTAEEAYNW SQGRAVFASG SPFDPVEYNG KIHVPGQSNN AYIFPGFGLG
560 570 580 590 600
VVISGAVRVH EDMLLAASET LADQATQENF EKGSIFPPFT NIRKISARIA
610 620 630
ASVAAKAYEL GLATRLPQPR DLEKYAESCM YTPVYRSYR
Length:639
Mass (Da):69,866
Last modified:May 30, 2006 - v2
Checksum:iA12C763D31D8D25D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451Missing in BAA03949 (PubMed:8204833).Curated
Sequence conflicti50 – 523AAA → TPV in BAA03949 (PubMed:8204833).Curated
Sequence conflicti494 – 4941S → A in BAA03949 (PubMed:8204833).Curated
Sequence conflicti602 – 6021S → T in BAA03949 (PubMed:8204833).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16499 mRNA. Translation: BAA03949.1.
AP002816 Genomic DNA. Translation: BAB03427.1.
AP002836 Genomic DNA. Translation: BAB07934.1.
PIRiS46499.
RefSeqiNP_001042257.1. NM_001048792.1.
UniGeneiOs.12492.

Genome annotation databases

EnsemblPlantsiOS01T0188400-01; OS01T0188400-01; OS01G0188400.
GeneIDi4326769.
KEGGiosa:4326769.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16499 mRNA. Translation: BAA03949.1.
AP002816 Genomic DNA. Translation: BAB03427.1.
AP002836 Genomic DNA. Translation: BAB07934.1.
PIRiS46499.
RefSeqiNP_001042257.1. NM_001048792.1.
UniGeneiOs.12492.

3D structure databases

ProteinModelPortaliP43279.
SMRiP43279. Positions 99-639.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os01g09320.1.

Proteomic databases

PRIDEiP43279.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS01T0188400-01; OS01T0188400-01; OS01G0188400.
GeneIDi4326769.
KEGGiosa:4326769.

Organism-specific databases

GrameneiP43279.

Phylogenomic databases

eggNOGiCOG0281.
InParanoidiP43279.
KOiK00029.
OMAiEEAYNWT.

Enzyme and pathway databases

UniPathwayiUPA00322.

Gene expression databases

ExpressionAtlasiP43279. baseline.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a rice cDNA similar to a maize NADP-dependent malic enzyme."
    Fushimi T., Umeda M., Shimazaki T., Kato A., Toriyama K., Uchimiya H.
    Plant Mol. Biol. 24:965-967(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Callus.
  2. "The genome sequence and structure of rice chromosome 1."
    Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
    , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
    Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiMAOC_ORYSJ
AccessioniPrimary (citable) accession number: P43279
Secondary accession number(s): Q9LDH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 30, 2006
Last modified: June 24, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.