P43279 (MAOC_ORYSJ) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 93.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: NADP-dependent malic enzyme, chloroplastic Short name=NADP-ME EC=1.1.1.40 | ||||||
| Gene names |
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| Organism | Oryza sativa subsp. japonica (Rice) [Reference proteome] | ||||||
| Taxonomic identifier | 39947 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza ›  |
Protein attributes
| Sequence length | 639 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | The chloroplastic ME isoform decarboxylates malate shuttled from neighboring mesophyll cells. The CO2 released is then refixed by ribulose-bisphosphate carboxylase. This pathway eliminates the photorespiratory loss of CO2 that occurs in most plants. |
| Catalytic activity | (S)-malate + NADP+ = pyruvate + CO2 + NADPH. Oxaloacetate = pyruvate + CO2. |
| Cofactor | Divalent metal cations. Prefers magnesium or manganese By similarity. |
| Pathway | |
| Subunit structure | Homotetramer By similarity. |
| Subcellular location | |
| Sequence similarities | Belongs to the malic enzymes family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 49 | 49 | Chloroplast Potential | ||||||
| Chain | 50 – 639 | 590 | NADP-dependent malic enzyme, chloroplastic | PRO_0000018548 | |||||
Regions | |||||||||
| Nucleotide binding | 383 – 399 | 17 | NADP By similarity | ||||||
Sites | |||||||||
| Active site | 187 | 1 | Proton donor By similarity | ||||||
| Active site | 258 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 330 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 331 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 354 | 1 | Divalent metal cation By similarity | ||||||
| Binding site | 240 | 1 | NAD By similarity | ||||||
| Binding site | 354 | 1 | NAD By similarity | ||||||
| Binding site | 495 | 1 | NAD By similarity | ||||||
| Site | 354 | 1 | Important for activity By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 45 | 1 | Missing in BAA03949. Ref.1 | ||||||
| Sequence conflict | 50 – 52 | 3 | AAA → TPV in BAA03949. Ref.1 | ||||||
| Sequence conflict | 494 | 1 | S → A in BAA03949. Ref.1 | ||||||
| Sequence conflict | 602 | 1 | S → T in BAA03949. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Nucleotide sequence of a rice cDNA similar to a maize NADP-dependent malic enzyme." Fushimi T., Umeda M., Shimazaki T., Kato A., Toriyama K., Uchimiya H. Plant Mol. Biol. 24:965-967(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Callus. |
| [2] | "The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.  Gojobori T.Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | D16499 mRNA. Translation: BAA03949.1. AP002816 Genomic DNA. Translation: BAB03427.1. AP002836 Genomic DNA. Translation: BAB07934.1. |
| PIR | S46499. |
| RefSeq | NP_001042257.1. NM_001048792.1. |
| UniGene | Os.12492. |
3D structure databases | |
| ProteinModelPortal | P43279. |
| SMR | P43279. Positions 99-639. |
| ModBase | Search... |
Proteomic databases | |
| PRIDE | P43279. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | LOC_Os01g09320.1; LOC_Os01g09320.1; LOC_Os01g09320. |
| GeneID | 4326769. |
| KEGG | osa:4326769. |
Organism-specific databases | |
| Gramene | P43279. |
Phylogenomic databases | |
| eggNOG | COG0281. |
| KO | K00029. |
| OMA | KLAVKIM. |
| ProtClustDB | PLN03129. |
Enzyme and pathway databases | |
| UniPathway | UPA00322. |
Gene expression databases | |
| ArrayExpress | P43279. |
Family and domain databases | |
| Gene3D | 3.40.50.10380. 1 hit. 3.40.50.720. 1 hit. |
| InterPro | IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD-bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Pfam | PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view] |
| PIRSF | PIRSF000106. ME. 1 hit. |
| PRINTS | PR00072. MALOXRDTASE. |
| SMART | SM00919. Malic_M. 1 hit. [Graphical view] |
| PROSITE | PS00331. MALIC_ENZYMES. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MAOC_ORYSJ | ||||||||
| Accession | Primary (citable) accession number: P43279 Secondary accession number(s): Q9LDH7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Oryza sativa (rice) Index of Oryza sativa entries and their corresponding gene designations |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |