Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P43279

- MAOC_ORYSJ

UniProt

P43279 - MAOC_ORYSJ

Protein

NADP-dependent malic enzyme, chloroplastic

Gene

ME6

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (30 May 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    The chloroplastic ME isoform decarboxylates malate shuttled from neighboring mesophyll cells. The CO2 released is then refixed by ribulose-bisphosphate carboxylase. This pathway eliminates the photorespiratory loss of CO2 that occurs in most plants.

    Catalytic activityi

    (S)-malate + NADP+ = pyruvate + CO2 + NADPH.
    Oxaloacetate = pyruvate + CO2.

    Cofactori

    Divalent metal cations. Prefers magnesium or manganese By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei187 – 1871Proton donorBy similarity
    Binding sitei240 – 2401NADBy similarity
    Active sitei258 – 2581Proton acceptorBy similarity
    Metal bindingi330 – 3301Divalent metal cationBy similarity
    Metal bindingi331 – 3311Divalent metal cationBy similarity
    Metal bindingi354 – 3541Divalent metal cationBy similarity
    Binding sitei354 – 3541NADBy similarity
    Sitei354 – 3541Important for activityBy similarity
    Binding sitei495 – 4951NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi383 – 39917NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. malate dehydrogenase (decarboxylating) (NAD+) activity Source: InterPro
    2. malate dehydrogenase (decarboxylating) (NADP+) activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. NAD binding Source: InterPro
    5. oxaloacetate decarboxylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. malate metabolic process Source: InterPro
    2. protein homooligomerization Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Metal-binding, NAD, NADP

    Enzyme and pathway databases

    ReactomeiREACT_206405. PPARA activates gene expression.
    UniPathwayiUPA00322.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADP-dependent malic enzyme, chloroplastic (EC:1.1.1.40)
    Short name:
    NADP-ME
    Gene namesi
    Name:ME6
    Ordered Locus Names:Os01g0188400, LOC_Os01g09320
    ORF Names:P0512G09.20, P0695A04.29
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 1

    Organism-specific databases

    GrameneiP43279.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell
    2. cytosol Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4949ChloroplastSequence AnalysisAdd
    BLAST
    Chaini50 – 639590NADP-dependent malic enzyme, chloroplasticPRO_0000018548Add
    BLAST

    Proteomic databases

    PRIDEiP43279.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP43279.
    SMRiP43279. Positions 99-639.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the malic enzymes family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0281.
    KOiK00029.
    OMAiEEAYTHT.

    Family and domain databases

    Gene3Di3.40.50.10380. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR015884. Malic_enzyme_CS.
    IPR012301. Malic_N_dom.
    IPR012302. Malic_NAD-bd.
    IPR001891. Malic_OxRdtase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00390. malic. 1 hit.
    PF03949. Malic_M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000106. ME. 1 hit.
    PRINTSiPR00072. MALOXRDTASE.
    SMARTiSM00919. Malic_M. 1 hit.
    [Graphical view]
    PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43279-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSARAAATA AAAAASPLWK RGEGGSSGSG SGCTSCREVR RRAAAVRVRA    50
    AAPRRVEAVA MESAAETEKK EEVAAAGGGV EDMATEEVPV TPWAFSVASG 100
    YTLLRDPHHN KGLAFSEKER DAHYLRGLLP PAVVSQDLQV KKIMHNLRQY 150
    SVPLQRYMAM MDLQERNERL FYKLLIDNVE ELLPVVYTPT VGEACQKYGS 200
    IFRQPQGLYV SLKDKGKVLD VLRNWPERNI QVIVVTDGER ILGLGDLGCQ 250
    GMGIPVGKLS LYTALGGVRP SACLPITIDV GTNNEQLLND EFYIGLRQRR 300
    ATGKEYHELM EEFMSAVKQI YGEKVLIQFE DFANHNAFDL LAKYSKSHLV 350
    FNDDIQGTAS VVLAGLLSSL KVVGGTLAEH TYLFLGAGEA GTGIAELIAL 400
    EISKQTKAPI EECRKKVWLL DSKGLIVNSR KESLQAFKKP WAHEHEPVTT 450
    LLDAVQSIKP TVLIGTSGVG KTFTKEVIEA MASFNERPVI FSLSNPTSHS 500
    ECTAEEAYNW SQGRAVFASG SPFDPVEYNG KIHVPGQSNN AYIFPGFGLG 550
    VVISGAVRVH EDMLLAASET LADQATQENF EKGSIFPPFT NIRKISARIA 600
    ASVAAKAYEL GLATRLPQPR DLEKYAESCM YTPVYRSYR 639
    Length:639
    Mass (Da):69,866
    Last modified:May 30, 2006 - v2
    Checksum:iA12C763D31D8D25D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti45 – 451Missing in BAA03949. (PubMed:8204833)Curated
    Sequence conflicti50 – 523AAA → TPV in BAA03949. (PubMed:8204833)Curated
    Sequence conflicti494 – 4941S → A in BAA03949. (PubMed:8204833)Curated
    Sequence conflicti602 – 6021S → T in BAA03949. (PubMed:8204833)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16499 mRNA. Translation: BAA03949.1.
    AP002816 Genomic DNA. Translation: BAB03427.1.
    AP002836 Genomic DNA. Translation: BAB07934.1.
    PIRiS46499.
    RefSeqiNP_001042257.1. NM_001048792.1.
    UniGeneiOs.12492.

    Genome annotation databases

    EnsemblPlantsiOS01T0188400-01; OS01T0188400-01; OS01G0188400.
    GeneIDi4326769.
    KEGGiosa:4326769.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16499 mRNA. Translation: BAA03949.1 .
    AP002816 Genomic DNA. Translation: BAB03427.1 .
    AP002836 Genomic DNA. Translation: BAB07934.1 .
    PIRi S46499.
    RefSeqi NP_001042257.1. NM_001048792.1.
    UniGenei Os.12492.

    3D structure databases

    ProteinModelPortali P43279.
    SMRi P43279. Positions 99-639.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P43279.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS01T0188400-01 ; OS01T0188400-01 ; OS01G0188400 .
    GeneIDi 4326769.
    KEGGi osa:4326769.

    Organism-specific databases

    Gramenei P43279.

    Phylogenomic databases

    eggNOGi COG0281.
    KOi K00029.
    OMAi EEAYTHT.

    Enzyme and pathway databases

    UniPathwayi UPA00322 .
    Reactomei REACT_206405. PPARA activates gene expression.

    Family and domain databases

    Gene3Di 3.40.50.10380. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR015884. Malic_enzyme_CS.
    IPR012301. Malic_N_dom.
    IPR012302. Malic_NAD-bd.
    IPR001891. Malic_OxRdtase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00390. malic. 1 hit.
    PF03949. Malic_M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000106. ME. 1 hit.
    PRINTSi PR00072. MALOXRDTASE.
    SMARTi SM00919. Malic_M. 1 hit.
    [Graphical view ]
    PROSITEi PS00331. MALIC_ENZYMES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of a rice cDNA similar to a maize NADP-dependent malic enzyme."
      Fushimi T., Umeda M., Shimazaki T., Kato A., Toriyama K., Uchimiya H.
      Plant Mol. Biol. 24:965-967(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Callus.
    2. "The genome sequence and structure of rice chromosome 1."
      Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
      , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
      Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiMAOC_ORYSJ
    AccessioniPrimary (citable) accession number: P43279
    Secondary accession number(s): Q9LDH7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: May 30, 2006
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3