NADP-dependent malic enzyme, chloroplastic precursor - Oryza sativa subsp. japonica (Rice)

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Reviewed, UniProtKB/Swiss-Prot P43279 (MAOC_ORYSJ)

Last modified June 16, 2009. Version 66. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    NADP-dependent malic enzyme, chloroplastic
      Short name=NADP-ME
    EC=1.1.1.40

Gene names

Name:	ME6
Ordered Locus Names:	Os01g0188400, LOC_Os01g09320
ORF Names:	P0512G09.20, P0695A04.29

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

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Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	The chloroplastic ME isoform decarboxylates malate shuttled from neighboring mesophyll cells. The CO₂ released is then refixed by ribulose-bisphosphate carboxylase. This pathway eliminates the photorespiratory loss of CO₂ that occurs in most plants.
Catalytic activity	(S)-malate + NADP⁺ = pyruvate + CO₂ + NADPH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Pathway	Photosynthesis; C4 acid pathway.
Subunit structure	Homotetramer By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the malic enzymes family.

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Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Metal-binding NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 49

Chloroplast Potential

Chain

50 – 639

590

NADP-dependent malic enzyme, chloroplastic

PRO_0000018548

Regions

Nucleotide binding

383 – 399

NADP By similarity

Sites

Active site

187

Proton donor By similarity

Active site

258

Proton acceptor By similarity

Metal binding

330

Divalent metal cation By similarity

Metal binding

331

Divalent metal cation By similarity

Metal binding

354

Divalent metal cation By similarity

Binding site

240

NAD By similarity

Binding site

354

NAD By similarity

Binding site

495

NAD By similarity

Site

354

Important for activity By similarity

Experimental info

Sequence conflict

Missing in BAA03949. Ref.1

Sequence conflict

50 – 52

AAA → TPV in BAA03949. Ref.1

Sequence conflict

494

S → A in BAA03949. Ref.1

Sequence conflict

602

S → T in BAA03949. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P43279-1 [UniParc].

Last modified May 30, 2006. Version 2.
Checksum: A12C763D31D8D25D
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FASTA

639

69,866

        10         20         30         40         50         60 
MLSARAAATA AAAAASPLWK RGEGGSSGSG SGCTSCREVR RRAAAVRVRA AAPRRVEAVA 

        70         80         90        100        110        120 
MESAAETEKK EEVAAAGGGV EDMATEEVPV TPWAFSVASG YTLLRDPHHN KGLAFSEKER 

       130        140        150        160        170        180 
DAHYLRGLLP PAVVSQDLQV KKIMHNLRQY SVPLQRYMAM MDLQERNERL FYKLLIDNVE 

       190        200        210        220        230        240 
ELLPVVYTPT VGEACQKYGS IFRQPQGLYV SLKDKGKVLD VLRNWPERNI QVIVVTDGER 

       250        260        270        280        290        300 
ILGLGDLGCQ GMGIPVGKLS LYTALGGVRP SACLPITIDV GTNNEQLLND EFYIGLRQRR 

       310        320        330        340        350        360 
ATGKEYHELM EEFMSAVKQI YGEKVLIQFE DFANHNAFDL LAKYSKSHLV FNDDIQGTAS 

       370        380        390        400        410        420 
VVLAGLLSSL KVVGGTLAEH TYLFLGAGEA GTGIAELIAL EISKQTKAPI EECRKKVWLL 

       430        440        450        460        470        480 
DSKGLIVNSR KESLQAFKKP WAHEHEPVTT LLDAVQSIKP TVLIGTSGVG KTFTKEVIEA 

       490        500        510        520        530        540 
MASFNERPVI FSLSNPTSHS ECTAEEAYNW SQGRAVFASG SPFDPVEYNG KIHVPGQSNN 

       550        560        570        580        590        600 
AYIFPGFGLG VVISGAVRVH EDMLLAASET LADQATQENF EKGSIFPPFT NIRKISARIA 

       610        620        630 
ASVAAKAYEL GLATRLPQPR DLEKYAESCM YTPVYRSYR

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a rice cDNA similar to a maize NADP-dependent malic enzyme." Fushimi T., Umeda M., Shimazaki T., Kato A., Toriyama K., Uchimiya H. Plant Mol. Biol. 24:965-967(1994) [PubMed: 8204833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Callus.
[2]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

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Cross-references

Sequence databases
	D16499 mRNA. Translation: BAA03949.1. AP002816 Genomic DNA. Translation: BAB03427.1. AP002836 Genomic DNA. Translation: BAB07934.1.
PIR	S46499.
RefSeq	NP_001042257.1.
UniGene	Os.12492
3D structure databases
HSSP	HSSP built from PDB template 1GQ2 based on UniProtKB P40927.
ModBase	Search...
Genome annotation databases
GeneID	4326769.
KEGG	osa:4326769.
Organism-specific databases
Gramene	P43279.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

MAOC_ORYSJ

Accession

Primary (citable) accession number: P43279
Secondary accession number(s): Q9LDH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	May 30, 2006
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Family and domain databases

Entry information

Relevant documents