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P43279 (MAOC_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADP-dependent malic enzyme, chloroplastic

Short name=NADP-ME
EC=1.1.1.40
Gene names
Name:ME6
Ordered Locus Names:Os01g0188400, LOC_Os01g09320
ORF Names:P0512G09.20, P0695A04.29
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length639 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The chloroplastic ME isoform decarboxylatesmalate shuttled from neighboring mesophyll cells. The CO2 released is then refixed by ribulose-bisphosphate carboxylase. This pathway eliminates the photorespiratory loss of CO2 that occurs in most plants.

Catalytic activity

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.

Oxaloacetate = pyruvate + CO2.

Cofactor

Divalent metal cations. Prefers magnesium or manganese By similarity.

Pathway

Photosynthesis; C4 acid pathway.

Subunit structure

Homotetramer By similarity.

Subcellular location

Plastidchloroplast.

Sequence similarities

Belongs to the malic enzymes family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4949Chloroplast Potential
Chain50 – 639590NADP-dependent malic enzyme, chloroplastic
PRO_0000018548

Regions

Nucleotide binding383 – 39917NADP By similarity

Sites

Active site1871Proton donor By similarity
Active site2581Proton acceptor By similarity
Metal binding3301Divalent metal cation By similarity
Metal binding3311Divalent metal cation By similarity
Metal binding3541Divalent metal cation By similarity
Binding site2401NAD By similarity
Binding site3541NAD By similarity
Binding site4951NAD By similarity
Site3541Important for activity By similarity

Experimental info

Sequence conflict451Missing in BAA03949. Ref.1
Sequence conflict50 – 523AAA → TPV in BAA03949. Ref.1
Sequence conflict4941S → A in BAA03949. Ref.1
Sequence conflict6021S → T in BAA03949. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P43279 [UniParc].

Last modified May 30, 2006. Version 2.
Checksum: A12C763D31D8D25D

FASTA63969,866
        10         20         30         40         50         60 
MLSARAAATA AAAAASPLWK RGEGGSSGSG SGCTSCREVR RRAAAVRVRA AAPRRVEAVA 

        70         80         90        100        110        120 
MESAAETEKK EEVAAAGGGV EDMATEEVPV TPWAFSVASG YTLLRDPHHN KGLAFSEKER 

       130        140        150        160        170        180 
DAHYLRGLLP PAVVSQDLQV KKIMHNLRQY SVPLQRYMAM MDLQERNERL FYKLLIDNVE 

       190        200        210        220        230        240 
ELLPVVYTPT VGEACQKYGS IFRQPQGLYV SLKDKGKVLD VLRNWPERNI QVIVVTDGER 

       250        260        270        280        290        300 
ILGLGDLGCQ GMGIPVGKLS LYTALGGVRP SACLPITIDV GTNNEQLLND EFYIGLRQRR 

       310        320        330        340        350        360 
ATGKEYHELM EEFMSAVKQI YGEKVLIQFE DFANHNAFDL LAKYSKSHLV FNDDIQGTAS 

       370        380        390        400        410        420 
VVLAGLLSSL KVVGGTLAEH TYLFLGAGEA GTGIAELIAL EISKQTKAPI EECRKKVWLL 

       430        440        450        460        470        480 
DSKGLIVNSR KESLQAFKKP WAHEHEPVTT LLDAVQSIKP TVLIGTSGVG KTFTKEVIEA 

       490        500        510        520        530        540 
MASFNERPVI FSLSNPTSHS ECTAEEAYNW SQGRAVFASG SPFDPVEYNG KIHVPGQSNN 

       550        560        570        580        590        600 
AYIFPGFGLG VVISGAVRVH EDMLLAASET LADQATQENF EKGSIFPPFT NIRKISARIA 

       610        620        630 
ASVAAKAYEL GLATRLPQPR DLEKYAESCM YTPVYRSYR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D16499 mRNA. Translation: BAA03949.1.
AP002816 Genomic DNA. Translation: BAB03427.1.
AP002836 Genomic DNA. Translation: BAB07934.1.
PIRS46499.
RefSeqNP_001042257.1. NM_001048792.1.
UniGeneOs.12492.

3D structure databases

ProteinModelPortalP43279.
SMRP43279. Positions 99-639.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP43279.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS01T0188400-01; OS01T0188400-01; OS01G0188400.
GeneID4326769.
KEGGosa:4326769.

Organism-specific databases

GrameneP43279.

Phylogenomic databases

eggNOGCOG0281.
KOK00029.
OMAEEAYTHT.

Enzyme and pathway databases

UniPathwayUPA00322.

Family and domain databases

Gene3D3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFPIRSF000106. ME. 1 hit.
PRINTSPR00072. MALOXRDTASE.
SMARTSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMAOC_ORYSJ
AccessionPrimary (citable) accession number: P43279
Secondary accession number(s): Q9LDH7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 30, 2006
Last modified: May 14, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways