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Protein

Histone H1.5

Gene

Hist1h1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).By similarity

GO - Molecular functioni

  • DNA binding Source: MGI

GO - Biological processi

  • muscle organ development Source: MGI
  • nucleosome assembly Source: InterPro
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H1.5
Alternative name(s):
H1 VAR.5
H1b
Gene namesi
Name:Hist1h1b
Synonyms:H1f5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1861461. Hist1h1b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001959182 – 223Histone H1.5Add BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineBy similarity1
Modified residuei17N6-acetyllysine; alternateCombined sources1
Cross-linki17Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei18PhosphoserineCombined sources1
Modified residuei26N6-acetyllysine; alternateBy similarity1
Modified residuei26N6-methyllysine; alternateBy similarity1
Modified residuei34N6-methyllysine; alternateBy similarity1
Modified residuei34N6-succinyllysine; alternateCombined sources1
Modified residuei36PhosphothreonineCombined sources1
Modified residuei41PhosphoserineBy similarity1
Modified residuei46N6-acetyllysineCombined sources1
Modified residuei54Citrulline1 Publication1
Modified residuei75N6-acetyllysineCombined sources1
Modified residuei90N6-acetyllysineBy similarity1
Modified residuei97N6-succinyllysineBy similarity1
Modified residuei104PhosphoserineBy similarity1
Modified residuei152PhosphothreonineBy similarity1
Modified residuei165N6-acetyllysineBy similarity1
Modified residuei170PhosphoserineBy similarity1
Modified residuei185N6-methyllysineBy similarity1
Modified residuei186PhosphoserineBy similarity1
Cross-linki216Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter.By similarity
Citrullination at Arg-54 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.1 Publication

Keywords - PTMi

Acetylation, Citrullination, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP43276.
PaxDbiP43276.
PeptideAtlasiP43276.
PRIDEiP43276.

PTM databases

iPTMnetiP43276.
PhosphoSitePlusiP43276.

Expressioni

Gene expression databases

BgeeiENSMUSG00000058773.
CleanExiMM_HIST1H1B.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Msx1P132973EBI-903960,EBI-903969

Protein-protein interaction databases

BioGridi208131. 3 interactors.
IntActiP43276. 5 interactors.
MINTiMINT-1868570.
STRINGi10090.ENSMUSP00000079356.

Structurei

3D structure databases

ProteinModelPortaliP43276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 109H15PROSITE-ProRule annotationAdd BLAST74

Domaini

The C-terminal domain is required for high-affinity binding to chromatin.By similarity

Sequence similaritiesi

Belongs to the histone H1/H5 family.PROSITE-ProRule annotation
Contains 1 H15 (linker histone H1/H5 globular) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4012. Eukaryota.
ENOG4112541. LUCA.
GeneTreeiENSGT00670000097781.
HOGENOMiHOG000251627.
HOVERGENiHBG009035.
InParanoidiP43276.
KOiK11275.
OrthoDBiEOG091G0XGD.
TreeFamiTF313664.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005818. Histone_H1/H5_H15.
IPR005819. Histone_H5.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00538. Linker_histone. 1 hit.
[Graphical view]
PRINTSiPR00624. HISTONEH5.
SMARTiSM00526. H15. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS51504. H15. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43276-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETAPAETA APAPVEKSPA KKKTTKKAGA AKRKATGPPV SELITKAVSA
60 70 80 90 100
SKERGGVSLP ALKKALAAGG YDVEKNNSRI KLGLKSLVSK GTLVQTKGTG
110 120 130 140 150
ASGSFKLNKK AASGEAKPKA KKTGAAKAKK PAGATPKKPK KTAGAKKTVK
160 170 180 190 200
KTPKKAKKPA AAGVKKVAKS PKKAKAAAKP KKAAKSPAKP KAVKSKASKP
210 220
KVTKPKTAKP KAAKAKKAVS KKK
Length:223
Mass (Da):22,576
Last modified:January 23, 2007 - v2
Checksum:iB9C26AC31C2716B6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61A → T in BAB32001 (PubMed:12408966).Curated1
Sequence conflicti120A → V in BAB32001 (PubMed:12408966).Curated1
Sequence conflicti137K → N in BAB32001 (PubMed:12408966).Curated1
Sequence conflicti195S → F in BAB32001 (PubMed:12408966).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46227 Genomic DNA. Translation: CAA86299.1.
U62922 Genomic DNA. Translation: AAB05798.1.
AY158904 Genomic DNA. Translation: AAO06215.1.
AK020117 mRNA. Translation: BAB32001.1.
CCDSiCCDS26295.1.
PIRiA35245.
S49492.
RefSeqiNP_064418.1. NM_020034.2.
UniGeneiMm.221314.

Genome annotation databases

EnsembliENSMUST00000080511; ENSMUSP00000079356; ENSMUSG00000058773.
GeneIDi56702.
KEGGimmu:56702.
UCSCiuc007pri.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46227 Genomic DNA. Translation: CAA86299.1.
U62922 Genomic DNA. Translation: AAB05798.1.
AY158904 Genomic DNA. Translation: AAO06215.1.
AK020117 mRNA. Translation: BAB32001.1.
CCDSiCCDS26295.1.
PIRiA35245.
S49492.
RefSeqiNP_064418.1. NM_020034.2.
UniGeneiMm.221314.

3D structure databases

ProteinModelPortaliP43276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208131. 3 interactors.
IntActiP43276. 5 interactors.
MINTiMINT-1868570.
STRINGi10090.ENSMUSP00000079356.

PTM databases

iPTMnetiP43276.
PhosphoSitePlusiP43276.

Proteomic databases

EPDiP43276.
PaxDbiP43276.
PeptideAtlasiP43276.
PRIDEiP43276.

Protocols and materials databases

DNASUi56702.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080511; ENSMUSP00000079356; ENSMUSG00000058773.
GeneIDi56702.
KEGGimmu:56702.
UCSCiuc007pri.2. mouse.

Organism-specific databases

CTDi3009.
MGIiMGI:1861461. Hist1h1b.

Phylogenomic databases

eggNOGiKOG4012. Eukaryota.
ENOG4112541. LUCA.
GeneTreeiENSGT00670000097781.
HOGENOMiHOG000251627.
HOVERGENiHBG009035.
InParanoidiP43276.
KOiK11275.
OrthoDBiEOG091G0XGD.
TreeFamiTF313664.

Miscellaneous databases

PROiP43276.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000058773.
CleanExiMM_HIST1H1B.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005818. Histone_H1/H5_H15.
IPR005819. Histone_H5.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00538. Linker_histone. 1 hit.
[Graphical view]
PRINTSiPR00624. HISTONEH5.
SMARTiSM00526. H15. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS51504. H15. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiH15_MOUSE
AccessioniPrimary (citable) accession number: P43276
Secondary accession number(s): Q9CRM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.