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Protein

ETS translocation variant 4

Gene

ETV4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi341 – 421ETSPROSITE-ProRule annotationAdd BLAST81

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000175832-MONOMER.
ReactomeiR-HSA-5687128. MAPK6/MAPK4 signaling.
SignaLinkiP43268.
SIGNORiP43268.

Names & Taxonomyi

Protein namesi
Recommended name:
ETS translocation variant 4
Alternative name(s):
Adenovirus E1A enhancer-binding protein
E1A-F
Polyomavirus enhancer activator 3 homolog
Short name:
Protein PEA3
Gene namesi
Name:ETV4
Synonyms:E1AF, PEA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:3493. ETV4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi96K → R: Altered sumoylation pattern. Loss of sumoylation, ubiquitination and transcriptional activator function; when associated with R-226 and R-260. 1 Publication1
Mutagenesisi98E → A: Loss of polysumoylation and ubiquitination; when associated with A-228; A-262; A-324 and A-443. 1 Publication1
Mutagenesisi101S → A: Loss of sumoylation at K-96. 1 Publication1
Mutagenesisi101S → E: Normal sumoylation at K-96. 1 Publication1
Mutagenesisi102P → A: Loss of sumoylation at K-96. 1 Publication1
Mutagenesisi226K → R: Altered sumoylation pattern. Loss of sumoylation, ubiquitination and transcriptional activator function; when associated with R-96 and R-260. 1 Publication1
Mutagenesisi228E → A: Loss of polysumoylation and ubiquitination; when associated with A-98; A-262; A-324 and A-443. 1 Publication1
Mutagenesisi260K → R: Altered sumoylation pattern. Loss of sumoylation, ubiquitination and transcriptional activator function; when associated with R-96 and R-226. 1 Publication1
Mutagenesisi262E → A: Loss of polysumoylation and ubiquitination; when associated with A-98; A-228; A-324 and A-443. 1 Publication1
Mutagenesisi324E → A: Loss of polysumoylation and ubiquitination; when associated with A-98; A-228; A-262 and A-443. 1 Publication1
Mutagenesisi443E → A: Loss of polysumoylation and ubiquitination; when associated with A-98; A-228; A-262 and A-324. 1 Publication1

Organism-specific databases

DisGeNETi2118.
MalaCardsiETV4.
OpenTargetsiENSG00000175832.
Orphaneti319. Ewing sarcoma.
PharmGKBiPA27907.

Polymorphism and mutation databases

BioMutaiETV4.
DMDMi62512145.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041161 – 484ETS translocation variant 4Add BLAST484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki96Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Modified residuei101Phosphoserine1 Publication1
Modified residuei140PhosphoserineCombined sources1
Modified residuei149PhosphoserineCombined sources1
Modified residuei214PhosphoserineBy similarity1
Cross-linki226Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Cross-linki260Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Cross-linki322Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP43268.
MaxQBiP43268.
PaxDbiP43268.
PeptideAtlasiP43268.
PRIDEiP43268.

PTM databases

iPTMnetiP43268.
PhosphoSitePlusiP43268.

Expressioni

Gene expression databases

BgeeiENSG00000175832.
CleanExiHS_ETV4.
ExpressionAtlasiP43268. baseline and differential.
GenevisibleiP43268. HS.

Organism-specific databases

HPAiHPA005768.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
taxP140793EBI-6447147,EBI-9675698From a different organism.

Protein-protein interaction databases

BioGridi108419. 12 interactors.
DIPiDIP-748N.
IntActiP43268. 6 interactors.
STRINGi9606.ENSP00000321835.

Structurei

Secondary structure

1484
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi343 – 352Combined sources10
Helixi354 – 356Combined sources3
Turni357 – 359Combined sources3
Beta strandi360 – 362Combined sources3
Beta strandi368 – 373Combined sources6
Helixi374 – 385Combined sources12
Helixi392 – 404Combined sources13
Beta strandi407 – 410Combined sources4
Beta strandi415 – 420Combined sources6
Helixi424 – 431Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CO8X-ray1.05A338-470[»]
4UUVX-ray2.80A/D/G/J/M/P/S/V338-435[»]
ProteinModelPortaliP43268.
SMRiP43268.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi49 – 75Asp/Glu-rich (acidic)Add BLAST27
Compositional biasi148 – 244Gln-richAdd BLAST97

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
GeneTreeiENSGT00760000118907.
HOGENOMiHOG000230986.
HOVERGENiHBG000231.
InParanoidiP43268.
KOiK15592.
OMAiPYPQQSF.
OrthoDBiEOG091G04YU.
PhylomeDBiP43268.
TreeFamiTF316214.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR006715. ETS_PEA3_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF04621. ETS_PEA3_N. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P43268-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERRMKAGYL DQQVPYTFSS KSPGNGSLRE ALIGPLGKLM DPGSLPPLDS
60 70 80 90 100
EDLFQDLSHF QETWLAEAQV PDSDEQFVPD FHSENLAFHS PTTRIKKEPQ
110 120 130 140 150
SPRTDPALSC SRKPPLPYHH GEQCLYSSAY DPPRQIAIKS PAPGALGQSP
160 170 180 190 200
LQPFPRAEQR NFLRSSGTSQ PHPGHGYLGE HSSVFQQPLD ICHSFTSQGG
210 220 230 240 250
GREPLPAPYQ HQLSEPCPPY PQQSFKQEYH DPLYEQAGQP AVDQGGVNGH
260 270 280 290 300
RYPGAGVVIK QEQTDFAYDS DVTGCASMYL HTEGFSGPSP GDGAMGYGYE
310 320 330 340 350
KPLRPFPDDV CVVPEKFEGD IKQEGVGAFR EGPPYQRRGA LQLWQFLVAL
360 370 380 390 400
LDDPTNAHFI AWTGRGMEFK LIEPEEVARL WGIQKNRPAM NYDKLSRSLR
410 420 430 440 450
YYYEKGIMQK VAGERYVYKF VCEPEALFSL AFPDNQRPAL KAEFDRPVSE
460 470 480
EDTVPLSHLD ESPAYLPELA GPAQPFGPKG GYSY
Length:484
Mass (Da):53,938
Last modified:April 12, 2005 - v3
Checksum:iBA9864F3C690A8C1
GO
Isoform 2 (identifier: P43268-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-39: Missing.

Note: No experimental confirmation available.
Show »
Length:445
Mass (Da):49,659
Checksum:i391745C2ADC1C064
GO
Isoform 3 (identifier: P43268-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-277: Missing.

Note: No experimental confirmation available.
Show »
Length:207
Mass (Da):23,422
Checksum:iF06C459C28B09DF5
GO

Sequence cautioni

The sequence AAA95991 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24 – 27GNGS → EMSD in BAA02234 (PubMed:8441666).Curated4

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_069110195F → I.1 PublicationCorresponds to variant rs150119757dbSNPEnsembl.1
Natural variantiVAR_048950437R → C.Corresponds to variant rs34260468dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0553141 – 277Missing in isoform 3. 1 PublicationAdd BLAST277
Alternative sequenceiVSP_0460361 – 39Missing in isoform 2. 1 PublicationAdd BLAST39

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18018 mRNA. Translation: AAA95991.1. Different initiation.
AF095890
, AF095887, AF095888, AF095889 Genomic DNA. Translation: AAD09186.1.
AK290429 mRNA. Translation: BAF83118.1.
AK299019 mRNA. Translation: BAH12929.1.
AK315961 mRNA. Translation: BAH14332.1.
AC068675 Genomic DNA. No translation available.
BC007242 mRNA. No translation available.
BC016623 mRNA. Translation: AAH16623.1.
D12765 mRNA. Translation: BAA02234.1.
CCDSiCCDS11465.1. [P43268-1]
CCDS58553.1. [P43268-2]
CCDS59292.1. [P43268-3]
PIRiS35534.
RefSeqiNP_001073143.1. NM_001079675.2. [P43268-1]
NP_001248366.1. NM_001261437.1. [P43268-2]
NP_001248367.1. NM_001261438.1. [P43268-2]
NP_001248368.1. NM_001261439.1. [P43268-3]
NP_001977.1. NM_001986.2. [P43268-1]
UniGeneiHs.434059.

Genome annotation databases

EnsembliENST00000319349; ENSP00000321835; ENSG00000175832. [P43268-1]
ENST00000393664; ENSP00000377273; ENSG00000175832. [P43268-1]
ENST00000538265; ENSP00000443846; ENSG00000175832. [P43268-2]
ENST00000545954; ENSP00000440023; ENSG00000175832. [P43268-2]
ENST00000586826; ENSP00000468636; ENSG00000175832. [P43268-3]
ENST00000591713; ENSP00000465718; ENSG00000175832. [P43268-1]
GeneIDi2118.
KEGGihsa:2118.
UCSCiuc002idv.5. human. [P43268-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18018 mRNA. Translation: AAA95991.1. Different initiation.
AF095890
, AF095887, AF095888, AF095889 Genomic DNA. Translation: AAD09186.1.
AK290429 mRNA. Translation: BAF83118.1.
AK299019 mRNA. Translation: BAH12929.1.
AK315961 mRNA. Translation: BAH14332.1.
AC068675 Genomic DNA. No translation available.
BC007242 mRNA. No translation available.
BC016623 mRNA. Translation: AAH16623.1.
D12765 mRNA. Translation: BAA02234.1.
CCDSiCCDS11465.1. [P43268-1]
CCDS58553.1. [P43268-2]
CCDS59292.1. [P43268-3]
PIRiS35534.
RefSeqiNP_001073143.1. NM_001079675.2. [P43268-1]
NP_001248366.1. NM_001261437.1. [P43268-2]
NP_001248367.1. NM_001261438.1. [P43268-2]
NP_001248368.1. NM_001261439.1. [P43268-3]
NP_001977.1. NM_001986.2. [P43268-1]
UniGeneiHs.434059.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CO8X-ray1.05A338-470[»]
4UUVX-ray2.80A/D/G/J/M/P/S/V338-435[»]
ProteinModelPortaliP43268.
SMRiP43268.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108419. 12 interactors.
DIPiDIP-748N.
IntActiP43268. 6 interactors.
STRINGi9606.ENSP00000321835.

PTM databases

iPTMnetiP43268.
PhosphoSitePlusiP43268.

Polymorphism and mutation databases

BioMutaiETV4.
DMDMi62512145.

Proteomic databases

EPDiP43268.
MaxQBiP43268.
PaxDbiP43268.
PeptideAtlasiP43268.
PRIDEiP43268.

Protocols and materials databases

DNASUi2118.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000319349; ENSP00000321835; ENSG00000175832. [P43268-1]
ENST00000393664; ENSP00000377273; ENSG00000175832. [P43268-1]
ENST00000538265; ENSP00000443846; ENSG00000175832. [P43268-2]
ENST00000545954; ENSP00000440023; ENSG00000175832. [P43268-2]
ENST00000586826; ENSP00000468636; ENSG00000175832. [P43268-3]
ENST00000591713; ENSP00000465718; ENSG00000175832. [P43268-1]
GeneIDi2118.
KEGGihsa:2118.
UCSCiuc002idv.5. human. [P43268-1]

Organism-specific databases

CTDi2118.
DisGeNETi2118.
GeneCardsiETV4.
HGNCiHGNC:3493. ETV4.
HPAiHPA005768.
MalaCardsiETV4.
MIMi600711. gene.
neXtProtiNX_P43268.
OpenTargetsiENSG00000175832.
Orphaneti319. Ewing sarcoma.
PharmGKBiPA27907.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
GeneTreeiENSGT00760000118907.
HOGENOMiHOG000230986.
HOVERGENiHBG000231.
InParanoidiP43268.
KOiK15592.
OMAiPYPQQSF.
OrthoDBiEOG091G04YU.
PhylomeDBiP43268.
TreeFamiTF316214.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000175832-MONOMER.
ReactomeiR-HSA-5687128. MAPK6/MAPK4 signaling.
SignaLinkiP43268.
SIGNORiP43268.

Miscellaneous databases

ChiTaRSiETV4. human.
GeneWikiiETV4.
GenomeRNAii2118.
PROiP43268.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000175832.
CleanExiHS_ETV4.
ExpressionAtlasiP43268. baseline and differential.
GenevisibleiP43268. HS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR006715. ETS_PEA3_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF04621. ETS_PEA3_N. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiETV4_HUMAN
AccessioniPrimary (citable) accession number: P43268
Secondary accession number(s): A8K314
, B7Z5J3, B7Z9J6, Q96AW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 12, 2005
Last modified: November 30, 2016
This is version 146 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.