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Protein

ETS translocation variant 4

Gene

ETV4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi341 – 421ETSPROSITE-ProRule annotationAdd BLAST81

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-5687128 MAPK6/MAPK4 signaling
SignaLinkiP43268
SIGNORiP43268

Names & Taxonomyi

Protein namesi
Recommended name:
ETS translocation variant 4
Alternative name(s):
Adenovirus E1A enhancer-binding protein
E1A-F
Polyomavirus enhancer activator 3 homolog
Short name:
Protein PEA3
Gene namesi
Name:ETV4
Synonyms:E1AF, PEA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000175832.12
HGNCiHGNC:3493 ETV4
MIMi600711 gene
neXtProtiNX_P43268

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi96K → R: Altered sumoylation pattern. Loss of sumoylation, ubiquitination and transcriptional activator function; when associated with R-226 and R-260. 1 Publication1
Mutagenesisi98E → A: Loss of polysumoylation and ubiquitination; when associated with A-228; A-262; A-324 and A-443. 1 Publication1
Mutagenesisi101S → A: Loss of sumoylation at K-96. 1 Publication1
Mutagenesisi101S → E: Normal sumoylation at K-96. 1 Publication1
Mutagenesisi102P → A: Loss of sumoylation at K-96. 1 Publication1
Mutagenesisi226K → R: Altered sumoylation pattern. Loss of sumoylation, ubiquitination and transcriptional activator function; when associated with R-96 and R-260. 1 Publication1
Mutagenesisi228E → A: Loss of polysumoylation and ubiquitination; when associated with A-98; A-262; A-324 and A-443. 1 Publication1
Mutagenesisi260K → R: Altered sumoylation pattern. Loss of sumoylation, ubiquitination and transcriptional activator function; when associated with R-96 and R-226. 1 Publication1
Mutagenesisi262E → A: Loss of polysumoylation and ubiquitination; when associated with A-98; A-228; A-324 and A-443. 1 Publication1
Mutagenesisi324E → A: Loss of polysumoylation and ubiquitination; when associated with A-98; A-228; A-262 and A-443. 1 Publication1
Mutagenesisi443E → A: Loss of polysumoylation and ubiquitination; when associated with A-98; A-228; A-262 and A-324. 1 Publication1

Organism-specific databases

DisGeNETi2118
MalaCardsiETV4
OpenTargetsiENSG00000175832
Orphaneti319 Ewing sarcoma
PharmGKBiPA27907

Polymorphism and mutation databases

BioMutaiETV4
DMDMi62512145

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041161 – 484ETS translocation variant 4Add BLAST484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki6Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki96Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Modified residuei101Phosphoserine1 Publication1
Cross-linki139Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei140PhosphoserineCombined sources1
Modified residuei149PhosphoserineCombined sources1
Modified residuei214PhosphoserineBy similarity1
Cross-linki226Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Cross-linki260Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Cross-linki322Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP43268
MaxQBiP43268
PaxDbiP43268
PeptideAtlasiP43268
PRIDEiP43268
ProteomicsDBi55606

PTM databases

iPTMnetiP43268
PhosphoSitePlusiP43268

Expressioni

Gene expression databases

BgeeiENSG00000175832
CleanExiHS_ETV4
ExpressionAtlasiP43268 baseline and differential
GenevisibleiP43268 HS

Organism-specific databases

HPAiHPA005768

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
taxP140793EBI-6447147,EBI-9675698From Human T-cell leukemia virus 1 (isolate Caribbea HS-35 subtype A).

Protein-protein interaction databases

BioGridi108419, 13 interactors
DIPiDIP-748N
IntActiP43268, 6 interactors
MINTiP43268
STRINGi9606.ENSP00000321835

Structurei

Secondary structure

1484
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi343 – 352Combined sources10
Helixi354 – 356Combined sources3
Turni357 – 359Combined sources3
Beta strandi360 – 362Combined sources3
Beta strandi368 – 373Combined sources6
Helixi374 – 385Combined sources12
Helixi392 – 404Combined sources13
Beta strandi407 – 410Combined sources4
Beta strandi415 – 420Combined sources6
Helixi424 – 431Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CO8X-ray1.05A338-470[»]
4UUVX-ray2.80A/D/G/J/M/P/S/V338-435[»]
5ILUX-ray1.10A340-436[»]
ProteinModelPortaliP43268
SMRiP43268
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi49 – 75Asp/Glu-rich (acidic)Add BLAST27
Compositional biasi148 – 244Gln-richAdd BLAST97

Sequence similaritiesi

Belongs to the ETS family.Curated

Phylogenomic databases

eggNOGiKOG3806 Eukaryota
ENOG410Z0ZF LUCA
GeneTreeiENSGT00760000118907
HOGENOMiHOG000230986
HOVERGENiHBG000231
InParanoidiP43268
KOiK15592
OMAiDFHSENL
OrthoDBiEOG091G04YU
PhylomeDBiP43268
TreeFamiTF316214

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000418 Ets_dom
IPR006715 ETS_PEA3_N
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00178 Ets, 1 hit
PF04621 ETS_PEA3_N, 1 hit
PRINTSiPR00454 ETSDOMAIN
SMARTiView protein in SMART
SM00413 ETS, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00345 ETS_DOMAIN_1, 1 hit
PS00346 ETS_DOMAIN_2, 1 hit
PS50061 ETS_DOMAIN_3, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P43268-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERRMKAGYL DQQVPYTFSS KSPGNGSLRE ALIGPLGKLM DPGSLPPLDS
60 70 80 90 100
EDLFQDLSHF QETWLAEAQV PDSDEQFVPD FHSENLAFHS PTTRIKKEPQ
110 120 130 140 150
SPRTDPALSC SRKPPLPYHH GEQCLYSSAY DPPRQIAIKS PAPGALGQSP
160 170 180 190 200
LQPFPRAEQR NFLRSSGTSQ PHPGHGYLGE HSSVFQQPLD ICHSFTSQGG
210 220 230 240 250
GREPLPAPYQ HQLSEPCPPY PQQSFKQEYH DPLYEQAGQP AVDQGGVNGH
260 270 280 290 300
RYPGAGVVIK QEQTDFAYDS DVTGCASMYL HTEGFSGPSP GDGAMGYGYE
310 320 330 340 350
KPLRPFPDDV CVVPEKFEGD IKQEGVGAFR EGPPYQRRGA LQLWQFLVAL
360 370 380 390 400
LDDPTNAHFI AWTGRGMEFK LIEPEEVARL WGIQKNRPAM NYDKLSRSLR
410 420 430 440 450
YYYEKGIMQK VAGERYVYKF VCEPEALFSL AFPDNQRPAL KAEFDRPVSE
460 470 480
EDTVPLSHLD ESPAYLPELA GPAQPFGPKG GYSY
Length:484
Mass (Da):53,938
Last modified:April 12, 2005 - v3
Checksum:iBA9864F3C690A8C1
GO
Isoform 2 (identifier: P43268-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-39: Missing.

Note: No experimental confirmation available.
Show »
Length:445
Mass (Da):49,659
Checksum:i391745C2ADC1C064
GO
Isoform 3 (identifier: P43268-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-277: Missing.

Note: No experimental confirmation available.
Show »
Length:207
Mass (Da):23,422
Checksum:iF06C459C28B09DF5
GO

Sequence cautioni

The sequence AAA95991 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24 – 27GNGS → EMSD in BAA02234 (PubMed:8441666).Curated4

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_069110195F → I1 PublicationCorresponds to variant dbSNP:rs150119757Ensembl.1
Natural variantiVAR_048950437R → C. Corresponds to variant dbSNP:rs34260468Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0553141 – 277Missing in isoform 3. 1 PublicationAdd BLAST277
Alternative sequenceiVSP_0460361 – 39Missing in isoform 2. 1 PublicationAdd BLAST39

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18018 mRNA Translation: AAA95991.1 Different initiation.
AF095890
, AF095887, AF095888, AF095889 Genomic DNA Translation: AAD09186.1
AK290429 mRNA Translation: BAF83118.1
AK299019 mRNA Translation: BAH12929.1
AK315961 mRNA Translation: BAH14332.1
AC068675 Genomic DNA No translation available.
BC007242 mRNA No translation available.
BC016623 mRNA Translation: AAH16623.1
D12765 mRNA Translation: BAA02234.1
CCDSiCCDS11465.1 [P43268-1]
CCDS58553.1 [P43268-2]
CCDS59292.1 [P43268-3]
PIRiS35534
RefSeqiNP_001073143.1, NM_001079675.2 [P43268-1]
NP_001248366.1, NM_001261437.1 [P43268-2]
NP_001248367.1, NM_001261438.1 [P43268-2]
NP_001248368.1, NM_001261439.1 [P43268-3]
NP_001977.1, NM_001986.2 [P43268-1]
UniGeneiHs.434059

Genome annotation databases

EnsembliENST00000319349; ENSP00000321835; ENSG00000175832 [P43268-1]
ENST00000393664; ENSP00000377273; ENSG00000175832 [P43268-1]
ENST00000538265; ENSP00000443846; ENSG00000175832 [P43268-2]
ENST00000545954; ENSP00000440023; ENSG00000175832 [P43268-2]
ENST00000586826; ENSP00000468636; ENSG00000175832 [P43268-3]
ENST00000591713; ENSP00000465718; ENSG00000175832 [P43268-1]
GeneIDi2118
KEGGihsa:2118
UCSCiuc002idv.5 human [P43268-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiETV4_HUMAN
AccessioniPrimary (citable) accession number: P43268
Secondary accession number(s): A8K314
, B7Z5J3, B7Z9J6, Q96AW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 12, 2005
Last modified: June 20, 2018
This is version 159 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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