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P43263

- NPRE_BREBE

UniProt

P43263 - NPRE_BREBE

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Protein

Bacillolysin

Gene
npr
Organism
Brevibacillus brevis (Bacillus brevis)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Binds 4 calcium ions per subunit Reviewed prediction.
Binds 1 zinc ion per subunit Reviewed prediction.

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi276 – 2761Calcium 1 Reviewed prediction
Metal bindingi278 – 2781Calcium 1 Reviewed prediction
Metal bindingi354 – 3541Calcium 2 Reviewed prediction
Metal bindingi358 – 3581Zinc; catalytic By similarity
Active sitei359 – 3591 By similarity
Metal bindingi362 – 3621Zinc; catalytic By similarity
Metal bindingi382 – 3821Zinc; catalytic By similarity
Metal bindingi393 – 3931Calcium 2 Reviewed prediction
Metal bindingi393 – 3931Calcium 3 Reviewed prediction
Metal bindingi394 – 3941Calcium 3; via carbonyl oxygen Reviewed prediction
Metal bindingi396 – 3961Calcium 2 Reviewed prediction
Metal bindingi396 – 3961Calcium 3 Reviewed prediction
Metal bindingi401 – 4011Calcium 2 Reviewed prediction
Metal bindingi401 – 4011Calcium 3 Reviewed prediction
Metal bindingi404 – 4041Calcium 4; via carbonyl oxygen Reviewed prediction
Metal bindingi405 – 4051Calcium 4 Reviewed prediction
Metal bindingi411 – 4111Calcium 4 Reviewed prediction
Active sitei442 – 4421Proton donor By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM04.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Neutral protease
Gene namesi
Name:npr
OrganismiBrevibacillus brevis (Bacillus brevis)
Taxonomic identifieri1393 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828 Reviewed predictionAdd
BLAST
Propeptidei29 – 223195Activation peptidePRO_0000028596Add
BLAST
Chaini224 – 527304BacillolysinPRO_0000028597Add
BLAST

Keywords - PTMi

Zymogen

Structurei

3D structure databases

ProteinModelPortaliP43263.
SMRiP43263. Positions 226-527.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43263-1 [UniParc]FASTAAdd to Basket

« Hide

MKKSYLATSL TLSIAVGVSG FTSVPAFAKT KIDYHKQWDT PQYIGEVWEP    50
EGAKGDDVVW SYLEKYKDEF RIQGNVEDHF EIVNEARNKE TDTKHYRLQE 100
VYNGIPIYGF QQTVHIDADG NVTSFLGQFI PDLDSNKQLK KKPKLNEQKA 150
VKQAIKDVEG EVGEKPDFIQ DPEAKLYIYV HEDESYLAYA VELNFLDPEP 200
GRWMYFIDAH SGDVINKYNM LDHVTATGKG VLGDTKQFET TKQGSTYMLK 250
DTTRGKGIET YTANNRTSLP GTLMTDSDNY WTDGAAVDAH AHAQKTYDYF 300
RNVHNRNSYD GNGAVIRSTV HYSTRYNNAF WNGSQMVYGD GDGTTFLPLS 350
GGLDVVAHEL THAVTERTAG LVYQNESGAL NESMSDIFGA MVDNDDWLMG 400
EDIYTPGRSG DALRSLQDPA AYGDPDHYSK RYTGSQDNGG VHTNSGINNK 450
AAYLLAEGGT HYGVRVNGIG RTDTAKIYYH ALTHYLTPYS NFSAMRRAAV 500
LSATDLFGAN SRQVQAVNAA YDAVGVK 527
Length:527
Mass (Da):58,646
Last modified:November 1, 1995 - v1
Checksum:i8D3704C3C9D8D756
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61286 Genomic DNA. Translation: CAA43589.1.
PIRiPN0114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61286 Genomic DNA. Translation: CAA43589.1 .
PIRi PN0114.

3D structure databases

ProteinModelPortali P43263.
SMRi P43263. Positions 226-527.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M04.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.170.10. 1 hit.
InterProi IPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view ]
Pfami PF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view ]
PRINTSi PR00730. THERMOLYSIN.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure of the Bacillus brevis metalloprotease gene."
    Avakov A.S., Bolotin A.P., Sorokin A.V.
    Mol. Biol. (Mosk.) 24:1363-1372(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 7882.
  2. "Analysis of the structure of Bacillus brevis neutral proteinase and its biosynthesis in Bacillus subtilis cells."
    Kaidalova N.V., Akimkina T.V., Khodova O.D., Kostrov S.V., Strongin A.Y.
    Mol. Biol. (Mosk.) 24:1381-1392(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 224-228, CHARACTERIZATION.

Entry informationi

Entry nameiNPRE_BREBE
AccessioniPrimary (citable) accession number: P43263
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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