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Protein

Bacillolysin

Gene

npr

Organism
Brevibacillus brevis (Bacillus brevis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+CuratedNote: Binds 4 Ca2+ ions per subunit.Curated
  • Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi276Calcium 1Sequence analysis1
Metal bindingi278Calcium 1Sequence analysis1
Metal bindingi354Calcium 2Sequence analysis1
Metal bindingi358Zinc; catalyticPROSITE-ProRule annotation1
Active sitei359PROSITE-ProRule annotation1
Metal bindingi362Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi382Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi393Calcium 2Sequence analysis1
Metal bindingi393Calcium 3Sequence analysis1
Metal bindingi394Calcium 3; via carbonyl oxygenSequence analysis1
Metal bindingi396Calcium 2Sequence analysis1
Metal bindingi396Calcium 3Sequence analysis1
Metal bindingi401Calcium 2Sequence analysis1
Metal bindingi401Calcium 3Sequence analysis1
Metal bindingi404Calcium 4; via carbonyl oxygenSequence analysis1
Metal bindingi405Calcium 4Sequence analysis1
Metal bindingi411Calcium 4Sequence analysis1
Active sitei442Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM04.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Neutral protease
Gene namesi
Name:npr
OrganismiBrevibacillus brevis (Bacillus brevis)
Taxonomic identifieri1393 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
PropeptideiPRO_000002859629 – 223Activation peptide1 PublicationAdd BLAST195
ChainiPRO_0000028597224 – 527BacillolysinAdd BLAST304

Keywords - PTMi

Zymogen

Structurei

3D structure databases

ProteinModelPortaliP43263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43263-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKSYLATSL TLSIAVGVSG FTSVPAFAKT KIDYHKQWDT PQYIGEVWEP
60 70 80 90 100
EGAKGDDVVW SYLEKYKDEF RIQGNVEDHF EIVNEARNKE TDTKHYRLQE
110 120 130 140 150
VYNGIPIYGF QQTVHIDADG NVTSFLGQFI PDLDSNKQLK KKPKLNEQKA
160 170 180 190 200
VKQAIKDVEG EVGEKPDFIQ DPEAKLYIYV HEDESYLAYA VELNFLDPEP
210 220 230 240 250
GRWMYFIDAH SGDVINKYNM LDHVTATGKG VLGDTKQFET TKQGSTYMLK
260 270 280 290 300
DTTRGKGIET YTANNRTSLP GTLMTDSDNY WTDGAAVDAH AHAQKTYDYF
310 320 330 340 350
RNVHNRNSYD GNGAVIRSTV HYSTRYNNAF WNGSQMVYGD GDGTTFLPLS
360 370 380 390 400
GGLDVVAHEL THAVTERTAG LVYQNESGAL NESMSDIFGA MVDNDDWLMG
410 420 430 440 450
EDIYTPGRSG DALRSLQDPA AYGDPDHYSK RYTGSQDNGG VHTNSGINNK
460 470 480 490 500
AAYLLAEGGT HYGVRVNGIG RTDTAKIYYH ALTHYLTPYS NFSAMRRAAV
510 520
LSATDLFGAN SRQVQAVNAA YDAVGVK
Length:527
Mass (Da):58,646
Last modified:November 1, 1995 - v1
Checksum:i8D3704C3C9D8D756
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61286 Genomic DNA. Translation: CAA43589.1.
PIRiPN0114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61286 Genomic DNA. Translation: CAA43589.1.
PIRiPN0114.

3D structure databases

ProteinModelPortaliP43263.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM04.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNPRE_BREBE
AccessioniPrimary (citable) accession number: P43263
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.