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P43263

- NPRE_BREBE

UniProt

P43263 - NPRE_BREBE

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Protein

Bacillolysin

Gene

npr

Organism
Brevibacillus brevis (Bacillus brevis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+CuratedNote: Binds 4 Ca(2+) ions per subunit.Curated
  • Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi276 – 2761Calcium 1Sequence Analysis
Metal bindingi278 – 2781Calcium 1Sequence Analysis
Metal bindingi354 – 3541Calcium 2Sequence Analysis
Metal bindingi358 – 3581Zinc; catalyticPROSITE-ProRule annotation
Active sitei359 – 3591PROSITE-ProRule annotation
Metal bindingi362 – 3621Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi382 – 3821Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi393 – 3931Calcium 2Sequence Analysis
Metal bindingi393 – 3931Calcium 3Sequence Analysis
Metal bindingi394 – 3941Calcium 3; via carbonyl oxygenSequence Analysis
Metal bindingi396 – 3961Calcium 2Sequence Analysis
Metal bindingi396 – 3961Calcium 3Sequence Analysis
Metal bindingi401 – 4011Calcium 2Sequence Analysis
Metal bindingi401 – 4011Calcium 3Sequence Analysis
Metal bindingi404 – 4041Calcium 4; via carbonyl oxygenSequence Analysis
Metal bindingi405 – 4051Calcium 4Sequence Analysis
Metal bindingi411 – 4111Calcium 4Sequence Analysis
Active sitei442 – 4421Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM04.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Neutral protease
Gene namesi
Name:npr
OrganismiBrevibacillus brevis (Bacillus brevis)
Taxonomic identifieri1393 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Propeptidei29 – 223195Activation peptide1 PublicationPRO_0000028596Add
BLAST
Chaini224 – 527304BacillolysinPRO_0000028597Add
BLAST

Keywords - PTMi

Zymogen

Structurei

3D structure databases

ProteinModelPortaliP43263.
SMRiP43263. Positions 226-527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43263-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKSYLATSL TLSIAVGVSG FTSVPAFAKT KIDYHKQWDT PQYIGEVWEP
60 70 80 90 100
EGAKGDDVVW SYLEKYKDEF RIQGNVEDHF EIVNEARNKE TDTKHYRLQE
110 120 130 140 150
VYNGIPIYGF QQTVHIDADG NVTSFLGQFI PDLDSNKQLK KKPKLNEQKA
160 170 180 190 200
VKQAIKDVEG EVGEKPDFIQ DPEAKLYIYV HEDESYLAYA VELNFLDPEP
210 220 230 240 250
GRWMYFIDAH SGDVINKYNM LDHVTATGKG VLGDTKQFET TKQGSTYMLK
260 270 280 290 300
DTTRGKGIET YTANNRTSLP GTLMTDSDNY WTDGAAVDAH AHAQKTYDYF
310 320 330 340 350
RNVHNRNSYD GNGAVIRSTV HYSTRYNNAF WNGSQMVYGD GDGTTFLPLS
360 370 380 390 400
GGLDVVAHEL THAVTERTAG LVYQNESGAL NESMSDIFGA MVDNDDWLMG
410 420 430 440 450
EDIYTPGRSG DALRSLQDPA AYGDPDHYSK RYTGSQDNGG VHTNSGINNK
460 470 480 490 500
AAYLLAEGGT HYGVRVNGIG RTDTAKIYYH ALTHYLTPYS NFSAMRRAAV
510 520
LSATDLFGAN SRQVQAVNAA YDAVGVK
Length:527
Mass (Da):58,646
Last modified:November 1, 1995 - v1
Checksum:i8D3704C3C9D8D756
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61286 Genomic DNA. Translation: CAA43589.1.
PIRiPN0114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61286 Genomic DNA. Translation: CAA43589.1 .
PIRi PN0114.

3D structure databases

ProteinModelPortali P43263.
SMRi P43263. Positions 226-527.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M04.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.170.10. 1 hit.
InterProi IPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view ]
Pfami PF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view ]
PRINTSi PR00730. THERMOLYSIN.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure of the Bacillus brevis metalloprotease gene."
    Avakov A.S., Bolotin A.P., Sorokin A.V.
    Mol. Biol. (Mosk.) 24:1363-1372(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 7882.
  2. "Analysis of the structure of Bacillus brevis neutral proteinase and its biosynthesis in Bacillus subtilis cells."
    Kaidalova N.V., Akimkina T.V., Khodova O.D., Kostrov S.V., Strongin A.Y.
    Mol. Biol. (Mosk.) 24:1381-1392(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 224-228, CHARACTERIZATION.

Entry informationi

Entry nameiNPRE_BREBE
AccessioniPrimary (citable) accession number: P43263
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3