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P43263 (NPRE_BREBE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bacillolysin

EC=3.4.24.28
Alternative name(s):
Neutral protease
Gene names
Name:npr
OrganismBrevibacillus brevis (Bacillus brevis)
Taxonomic identifier1393 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus

Protein attributes

Sequence length527 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Extracellular zinc metalloprotease.

Catalytic activity

Similar, but not identical, to that of thermolysin.

Cofactor

Binds 4 calcium ions per subunit Potential.

Binds 1 zinc ion per subunit Potential.

Subcellular location

Secreted.

Sequence similarities

Belongs to the peptidase M4 family.

Biophysicochemical properties

Temperature dependence:

Thermolabile.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMZymogen
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Propeptide29 – 223195Activation peptide
PRO_0000028596
Chain224 – 527304Bacillolysin
PRO_0000028597

Sites

Active site3591 By similarity
Active site4421Proton donor By similarity
Metal binding2761Calcium 1 Potential
Metal binding2781Calcium 1 Potential
Metal binding3541Calcium 2 Potential
Metal binding3581Zinc; catalytic By similarity
Metal binding3621Zinc; catalytic By similarity
Metal binding3821Zinc; catalytic By similarity
Metal binding3931Calcium 2 Potential
Metal binding3931Calcium 3 Potential
Metal binding3941Calcium 3; via carbonyl oxygen Potential
Metal binding3961Calcium 2 Potential
Metal binding3961Calcium 3 Potential
Metal binding4011Calcium 2 Potential
Metal binding4011Calcium 3 Potential
Metal binding4041Calcium 4; via carbonyl oxygen Potential
Metal binding4051Calcium 4 Potential
Metal binding4111Calcium 4 Potential

Sequences

Sequence LengthMass (Da)Tools
P43263 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 8D3704C3C9D8D756

FASTA52758,646
        10         20         30         40         50         60 
MKKSYLATSL TLSIAVGVSG FTSVPAFAKT KIDYHKQWDT PQYIGEVWEP EGAKGDDVVW 

        70         80         90        100        110        120 
SYLEKYKDEF RIQGNVEDHF EIVNEARNKE TDTKHYRLQE VYNGIPIYGF QQTVHIDADG 

       130        140        150        160        170        180 
NVTSFLGQFI PDLDSNKQLK KKPKLNEQKA VKQAIKDVEG EVGEKPDFIQ DPEAKLYIYV 

       190        200        210        220        230        240 
HEDESYLAYA VELNFLDPEP GRWMYFIDAH SGDVINKYNM LDHVTATGKG VLGDTKQFET 

       250        260        270        280        290        300 
TKQGSTYMLK DTTRGKGIET YTANNRTSLP GTLMTDSDNY WTDGAAVDAH AHAQKTYDYF 

       310        320        330        340        350        360 
RNVHNRNSYD GNGAVIRSTV HYSTRYNNAF WNGSQMVYGD GDGTTFLPLS GGLDVVAHEL 

       370        380        390        400        410        420 
THAVTERTAG LVYQNESGAL NESMSDIFGA MVDNDDWLMG EDIYTPGRSG DALRSLQDPA 

       430        440        450        460        470        480 
AYGDPDHYSK RYTGSQDNGG VHTNSGINNK AAYLLAEGGT HYGVRVNGIG RTDTAKIYYH 

       490        500        510        520 
ALTHYLTPYS NFSAMRRAAV LSATDLFGAN SRQVQAVNAA YDAVGVK 

« Hide

References

[1]"Structure of the Bacillus brevis metalloprotease gene."
Avakov A.S., Bolotin A.P., Sorokin A.V.
Mol. Biol. (Mosk.) 24:1363-1372(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 7882.
[2]"Analysis of the structure of Bacillus brevis neutral proteinase and its biosynthesis in Bacillus subtilis cells."
Kaidalova N.V., Akimkina T.V., Khodova O.D., Kostrov S.V., Strongin A.Y.
Mol. Biol. (Mosk.) 24:1381-1392(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 224-228, CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X61286 Genomic DNA. Translation: CAA43589.1.
PIRPN0114.

3D structure databases

ProteinModelPortalP43263.
SMRP43263. Positions 226-527.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSM04.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.10.170.10. 1 hit.
InterProIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSPR00730. THERMOLYSIN.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNPRE_BREBE
AccessionPrimary (citable) accession number: P43263
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries