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Protein

Intimin

Gene

eae

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for the production of attaching and effacing lesions on tissue culture cells.

GO - Biological processi

  • cell adhesion Source: InterPro
  • pathogenesis Source: CACAO
Complete GO annotation...

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciECOL386585:GJFA-4525-MONOMER.
ECOO157:EAE-MONOMER.

Protein family/group databases

TCDBi1.B.54.1.1. the intimin/invasin (int/inv) or autotransporter-3 (at-3) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Intimin
Alternative name(s):
Attaching and effacing protein
Short name:
Eae protein
Gamma-intimin
Gene namesi
Name:eae
Synonyms:eaeA
Ordered Locus Names:Z5110, ECs4559
ORF Names:L0025
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei20 – 3920HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 934934IntiminPRO_0000211828Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi155864.Z5110.

Structurei

Secondary structure

1
934
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi212 – 22110Combined sources
Beta strandi225 – 23612Combined sources
Beta strandi238 – 25114Combined sources
Beta strandi254 – 26613Combined sources
Beta strandi271 – 28111Combined sources
Turni282 – 2854Combined sources
Beta strandi286 – 29712Combined sources
Beta strandi300 – 30910Combined sources
Beta strandi317 – 3193Combined sources
Beta strandi323 – 3264Combined sources
Beta strandi329 – 33810Combined sources
Beta strandi341 – 35313Combined sources
Beta strandi355 – 3584Combined sources
Beta strandi363 – 3653Combined sources
Beta strandi367 – 3693Combined sources
Beta strandi371 – 38212Combined sources
Beta strandi385 – 39410Combined sources
Helixi395 – 3973Combined sources
Beta strandi398 – 41013Combined sources
Helixi415 – 4184Combined sources
Helixi423 – 4286Combined sources
Turni430 – 4323Combined sources
Beta strandi446 – 4483Combined sources
Beta strandi760 – 7645Combined sources
Turni765 – 7684Combined sources
Beta strandi769 – 7724Combined sources
Beta strandi775 – 7784Combined sources
Beta strandi782 – 7843Combined sources
Beta strandi787 – 7893Combined sources
Beta strandi794 – 7996Combined sources
Turni801 – 8033Combined sources
Beta strandi804 – 8063Combined sources
Beta strandi810 – 8145Combined sources
Beta strandi816 – 8183Combined sources
Beta strandi820 – 8267Combined sources
Turni827 – 8293Combined sources
Beta strandi831 – 8366Combined sources
Beta strandi842 – 8487Combined sources
Helixi851 – 8577Combined sources
Turni858 – 8603Combined sources
Helixi866 – 87611Combined sources
Helixi879 – 8813Combined sources
Helixi883 – 8853Combined sources
Beta strandi892 – 8943Combined sources
Helixi899 – 9046Combined sources
Beta strandi906 – 9116Combined sources
Turni912 – 9143Combined sources
Beta strandi917 – 9226Combined sources
Beta strandi929 – 9335Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZQKX-ray2.80A/B747-934[»]
2ZWKX-ray3.10A/C/E752-934[»]
3NCWX-ray2.80A/B/C/D747-934[»]
3NCXX-ray2.60A/B747-934[»]
4E1SX-ray1.86A208-449[»]
ProteinModelPortaliP43261.
SMRiP43261. Positions 61-116, 559-934.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43261.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 11250LysMPROSITE-ProRule annotationAdd
BLAST
Domaini560 – 65394Big-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini660 – 75394Big-1 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the intimin/invasin family.Curated
Contains 1 LysM domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105TNG. Bacteria.
ENOG410XQE6. LUCA.
HOGENOMiHOG000217915.
KOiK12790.
OMAiPENMLGY.

Family and domain databases

Gene3Di2.60.40.920. 2 hits.
3.10.100.10. 1 hit.
InterProiIPR003344. Big_1_dom.
IPR003343. Big_2.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
IPR003535. Intimin/invasin_bac.
IPR013117. Intimin_C.
IPR008964. Invasin/intimin_cell_adhesion.
IPR024519. Invasin_beta.
IPR018392. LysM_dom.
[Graphical view]
PfamiPF02369. Big_1. 2 hits.
PF02368. Big_2. 1 hit.
PF07979. Intimin_C. 1 hit.
PF11924. Invasin_beta. 1 hit.
PF01476. LysM. 1 hit.
[Graphical view]
PRINTSiPR01369. INTIMIN.
SMARTiSM00634. BID_1. 2 hits.
SM00635. BID_2. 1 hit.
SM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF49373. SSF49373. 3 hits.
SSF56436. SSF56436. 1 hit.
PROSITEiPS51127. BIG1. 2 hits.
PS51782. LYSM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITHGCYTRT RHKHKLKKTL IMLSAGLGLF FYVNQNSFAN GENYFKLGSD
60 70 80 90 100
SKLLTHDSYQ NRLFYTLKTG ETVADLSKSQ DINLSTIWSL NKHLYSSESE
110 120 130 140 150
MMKAAPGQQI ILPLKKLPFE YSALPLLGSA PLVAAGGVAG HTNKLTKMSP
160 170 180 190 200
DVTKSNMTDD KALNYAAQQA ASLGSQLQSR SLNGDYAKDT ALGIAGNQAS
210 220 230 240 250
SQLQAWLQHY GTAEVNLQSG NNFDGSSLDF LLPFYDSEKM LAFGQVGARY
260 270 280 290 300
IDSRFTANLG AGQRFFLPAN MLGYNVFIDQ DFSGDNTRLG IGGEYWRDYF
310 320 330 340 350
KSSVNGYFRM SGWHESYNKK DYDERPANGF DIRFNGYLPS YPALGAKLIY
360 370 380 390 400
EQYYGDNVAL FNSDKLQSNP GAATVGVNYT PIPLVTMGID YRHGTGNEND
410 420 430 440 450
LLYSMQFRYQ FDKSWSQQIE PQYVNELRTL SGSRYDLVQR NNNIILEYKK
460 470 480 490 500
QDILSLNIPH DINGTEHSTQ KIQLIVKSKY GLDRIVWDDS ALRSQGGQIQ
510 520 530 540 550
HSGSQSAQDY QAILPAYVQG GSNIYKVTAR AYDRNGNSSN NVQLTITVLS
560 570 580 590 600
NGQVVDQVGV TDFTADKTSA KADNADTITY TATVKKNGVA QANVPVSFNI
610 620 630 640 650
VSGTATLGAN SAKTDANGKA TVTLKSSTPG QVVVSAKTAE MTSALNASAV
660 670 680 690 700
IFFDQTKASI TEIKADKTTA VANGKDAIKY TVKVMKNGQP VNNQSVTFST
710 720 730 740 750
NFGMFNGKSQ TQATTGNDGR ATITLTSSSA GKATVSATVS DGAEVKATEV
760 770 780 790 800
TFFDELKIDN KVDIIGNNVR GELPNIWLQY GQFKLKASGG DGTYSWYSEN
810 820 830 840 850
TSIATVDASG KVTLNGKGSV VIKATSGDKQ TVSYTIKAPS YMIKVDKQAY
860 870 880 890 900
YADAMSICKN LLPSTQTVLS DIYDSWGAAN KYSHYSSMNS ITAWIKQTSS
910 920 930
EQRSGVSSTY NLITQNPLPG VNVNTPNVYA VCVE
Length:934
Mass (Da):101,836
Last modified:August 29, 2001 - v2
Checksum:iBE73686D7F79D3C9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti221 – 2211N → D in CAA77642 (PubMed:1552854).Curated
Sequence conflicti311 – 3122SG → RR in CAA77642 (PubMed:1552854).Curated
Sequence conflicti318 – 3181N → H in CAA77642 (PubMed:1552854).Curated
Sequence conflicti642 – 6421T → S in CAA77642 (PubMed:1552854).Curated
Sequence conflicti769 – 7691V → VK (PubMed:1577255).Curated
Sequence conflicti771 – 7722GE → SM (PubMed:1577255).Curated
Sequence conflicti903 – 9031R → S in AAA21468 (PubMed:8005211).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11541 Genomic DNA. Translation: CAA77642.1.
X60439 Genomic DNA. Translation: CAA42967.1.
AF071034 Genomic DNA. Translation: AAC31504.1.
AF081182 Genomic DNA. Translation: AAD05498.1.
AF081183 Genomic DNA. Translation: AAD05499.1.
AE005174 Genomic DNA. Translation: AAG58823.1.
BA000007 Genomic DNA. Translation: BAB37982.1.
U32312 Genomic DNA. Translation: AAB00111.1.
L08095 Genomic DNA. Translation: AAA21468.1.
PIRiC86045.
G91198.
I41193.
RefSeqiNP_312586.1. NC_002695.1.
WP_000627885.1. NZ_LMXL01000095.1.

Genome annotation databases

EnsemblBacteriaiAAG58823; AAG58823; Z5110.
BAB37982; BAB37982; BAB37982.
GeneIDi915471.
KEGGiece:Z5110.
ecs:ECs4559.
PATRICi18358741. VBIEscCol44059_4535.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11541 Genomic DNA. Translation: CAA77642.1.
X60439 Genomic DNA. Translation: CAA42967.1.
AF071034 Genomic DNA. Translation: AAC31504.1.
AF081182 Genomic DNA. Translation: AAD05498.1.
AF081183 Genomic DNA. Translation: AAD05499.1.
AE005174 Genomic DNA. Translation: AAG58823.1.
BA000007 Genomic DNA. Translation: BAB37982.1.
U32312 Genomic DNA. Translation: AAB00111.1.
L08095 Genomic DNA. Translation: AAA21468.1.
PIRiC86045.
G91198.
I41193.
RefSeqiNP_312586.1. NC_002695.1.
WP_000627885.1. NZ_LMXL01000095.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZQKX-ray2.80A/B747-934[»]
2ZWKX-ray3.10A/C/E752-934[»]
3NCWX-ray2.80A/B/C/D747-934[»]
3NCXX-ray2.60A/B747-934[»]
4E1SX-ray1.86A208-449[»]
ProteinModelPortaliP43261.
SMRiP43261. Positions 61-116, 559-934.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z5110.

Protein family/group databases

TCDBi1.B.54.1.1. the intimin/invasin (int/inv) or autotransporter-3 (at-3) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG58823; AAG58823; Z5110.
BAB37982; BAB37982; BAB37982.
GeneIDi915471.
KEGGiece:Z5110.
ecs:ECs4559.
PATRICi18358741. VBIEscCol44059_4535.

Phylogenomic databases

eggNOGiENOG4105TNG. Bacteria.
ENOG410XQE6. LUCA.
HOGENOMiHOG000217915.
KOiK12790.
OMAiPENMLGY.

Enzyme and pathway databases

BioCyciECOL386585:GJFA-4525-MONOMER.
ECOO157:EAE-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP43261.

Family and domain databases

Gene3Di2.60.40.920. 2 hits.
3.10.100.10. 1 hit.
InterProiIPR003344. Big_1_dom.
IPR003343. Big_2.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
IPR003535. Intimin/invasin_bac.
IPR013117. Intimin_C.
IPR008964. Invasin/intimin_cell_adhesion.
IPR024519. Invasin_beta.
IPR018392. LysM_dom.
[Graphical view]
PfamiPF02369. Big_1. 2 hits.
PF02368. Big_2. 1 hit.
PF07979. Intimin_C. 1 hit.
PF11924. Invasin_beta. 1 hit.
PF01476. LysM. 1 hit.
[Graphical view]
PRINTSiPR01369. INTIMIN.
SMARTiSM00634. BID_1. 2 hits.
SM00635. BID_2. 1 hit.
SM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF49373. SSF49373. 3 hits.
SSF56436. SSF56436. 1 hit.
PROSITEiPS51127. BIG1. 2 hits.
PS51782. LYSM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEAE_ECO57
AccessioniPrimary (citable) accession number: P43261
Secondary accession number(s): O85627, Q47168
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 29, 2001
Last modified: September 7, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.