Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Intimin

Gene

eae

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Necessary for the production of attaching and effacing lesions on tissue culture cells.

GO - Biological processi

  • cell adhesion Source: InterPro
  • pathogenesis Source: CACAO

Keywordsi

Biological processVirulence

Protein family/group databases

TCDBi1.B.54.1.1 the intimin/invasin (int/inv) or autotransporter-3 (at-3) family

Names & Taxonomyi

Protein namesi
Recommended name:
Intimin
Alternative name(s):
Attaching and effacing protein
Short name:
Eae protein
Gamma-intimin
Gene namesi
Name:eae
Synonyms:eaeA
Ordered Locus Names:Z5110, ECs4559
ORF Names:L0025
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei20 – 39HelicalSequence analysisAdd BLAST20

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002118281 – 934IntiminAdd BLAST934

Interactioni

Protein-protein interaction databases

STRINGi155864.Z5110

Structurei

Secondary structure

1934
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi212 – 221Combined sources10
Beta strandi225 – 236Combined sources12
Beta strandi238 – 251Combined sources14
Beta strandi254 – 266Combined sources13
Beta strandi271 – 281Combined sources11
Turni282 – 285Combined sources4
Beta strandi286 – 297Combined sources12
Beta strandi300 – 309Combined sources10
Beta strandi317 – 319Combined sources3
Beta strandi323 – 326Combined sources4
Beta strandi329 – 338Combined sources10
Beta strandi341 – 353Combined sources13
Beta strandi355 – 358Combined sources4
Beta strandi363 – 365Combined sources3
Beta strandi367 – 369Combined sources3
Beta strandi371 – 382Combined sources12
Beta strandi385 – 394Combined sources10
Helixi395 – 397Combined sources3
Beta strandi398 – 410Combined sources13
Helixi415 – 418Combined sources4
Helixi423 – 428Combined sources6
Turni430 – 432Combined sources3
Beta strandi446 – 448Combined sources3
Beta strandi760 – 764Combined sources5
Turni765 – 768Combined sources4
Beta strandi769 – 772Combined sources4
Beta strandi775 – 778Combined sources4
Beta strandi782 – 784Combined sources3
Beta strandi787 – 789Combined sources3
Beta strandi794 – 799Combined sources6
Turni801 – 803Combined sources3
Beta strandi804 – 806Combined sources3
Beta strandi810 – 814Combined sources5
Beta strandi816 – 818Combined sources3
Beta strandi820 – 826Combined sources7
Turni827 – 829Combined sources3
Beta strandi831 – 836Combined sources6
Beta strandi842 – 848Combined sources7
Helixi851 – 857Combined sources7
Turni858 – 860Combined sources3
Helixi866 – 876Combined sources11
Helixi879 – 881Combined sources3
Helixi883 – 885Combined sources3
Beta strandi892 – 894Combined sources3
Helixi899 – 904Combined sources6
Beta strandi906 – 911Combined sources6
Turni912 – 914Combined sources3
Beta strandi917 – 922Combined sources6
Beta strandi929 – 933Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZQKX-ray2.80A/B747-934[»]
2ZWKX-ray3.10A/C/E752-934[»]
3NCWX-ray2.80A/B/C/D747-934[»]
3NCXX-ray2.60A/B747-934[»]
4E1SX-ray1.86A208-449[»]
5G26X-ray2.42A208-449[»]
ProteinModelPortaliP43261
SMRiP43261
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43261

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 112LysMPROSITE-ProRule annotationAdd BLAST50
Domaini560 – 653Big-1 1PROSITE-ProRule annotationAdd BLAST94
Domaini660 – 753Big-1 2PROSITE-ProRule annotationAdd BLAST94

Sequence similaritiesi

Belongs to the intimin/invasin family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105TNG Bacteria
ENOG410XQE6 LUCA
HOGENOMiHOG000217915
KOiK12790
OMAiPENMLGY

Family and domain databases

Gene3Di2.40.160.160, 1 hit
2.60.40.10, 2 hits
3.10.100.10, 1 hit
InterProiView protein in InterPro
IPR003344 Big_1_dom
IPR003343 Big_2
IPR016186 C-type_lectin-like/link_sf
IPR016187 CTDL_fold
IPR024519 IAT_beta
IPR038177 IAT_beta_sf
IPR013783 Ig-like_fold
IPR003535 Intimin/invasin_bac
IPR013117 Intimin_C
IPR008964 Invasin/intimin_cell_adhesion
IPR018392 LysM_dom
PfamiView protein in Pfam
PF02369 Big_1, 2 hits
PF02368 Big_2, 1 hit
PF11924 IAT_beta, 1 hit
PF07979 Intimin_C, 1 hit
PF01476 LysM, 1 hit
PRINTSiPR01369 INTIMIN
SMARTiView protein in SMART
SM00634 BID_1, 2 hits
SM00635 BID_2, 1 hit
SM00257 LysM, 1 hit
SUPFAMiSSF49373 SSF49373, 3 hits
SSF56436 SSF56436, 1 hit
PROSITEiView protein in PROSITE
PS51127 BIG1, 2 hits
PS51782 LYSM, 1 hit

Sequencei

Sequence statusi: Complete.

P43261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITHGCYTRT RHKHKLKKTL IMLSAGLGLF FYVNQNSFAN GENYFKLGSD
60 70 80 90 100
SKLLTHDSYQ NRLFYTLKTG ETVADLSKSQ DINLSTIWSL NKHLYSSESE
110 120 130 140 150
MMKAAPGQQI ILPLKKLPFE YSALPLLGSA PLVAAGGVAG HTNKLTKMSP
160 170 180 190 200
DVTKSNMTDD KALNYAAQQA ASLGSQLQSR SLNGDYAKDT ALGIAGNQAS
210 220 230 240 250
SQLQAWLQHY GTAEVNLQSG NNFDGSSLDF LLPFYDSEKM LAFGQVGARY
260 270 280 290 300
IDSRFTANLG AGQRFFLPAN MLGYNVFIDQ DFSGDNTRLG IGGEYWRDYF
310 320 330 340 350
KSSVNGYFRM SGWHESYNKK DYDERPANGF DIRFNGYLPS YPALGAKLIY
360 370 380 390 400
EQYYGDNVAL FNSDKLQSNP GAATVGVNYT PIPLVTMGID YRHGTGNEND
410 420 430 440 450
LLYSMQFRYQ FDKSWSQQIE PQYVNELRTL SGSRYDLVQR NNNIILEYKK
460 470 480 490 500
QDILSLNIPH DINGTEHSTQ KIQLIVKSKY GLDRIVWDDS ALRSQGGQIQ
510 520 530 540 550
HSGSQSAQDY QAILPAYVQG GSNIYKVTAR AYDRNGNSSN NVQLTITVLS
560 570 580 590 600
NGQVVDQVGV TDFTADKTSA KADNADTITY TATVKKNGVA QANVPVSFNI
610 620 630 640 650
VSGTATLGAN SAKTDANGKA TVTLKSSTPG QVVVSAKTAE MTSALNASAV
660 670 680 690 700
IFFDQTKASI TEIKADKTTA VANGKDAIKY TVKVMKNGQP VNNQSVTFST
710 720 730 740 750
NFGMFNGKSQ TQATTGNDGR ATITLTSSSA GKATVSATVS DGAEVKATEV
760 770 780 790 800
TFFDELKIDN KVDIIGNNVR GELPNIWLQY GQFKLKASGG DGTYSWYSEN
810 820 830 840 850
TSIATVDASG KVTLNGKGSV VIKATSGDKQ TVSYTIKAPS YMIKVDKQAY
860 870 880 890 900
YADAMSICKN LLPSTQTVLS DIYDSWGAAN KYSHYSSMNS ITAWIKQTSS
910 920 930
EQRSGVSSTY NLITQNPLPG VNVNTPNVYA VCVE
Length:934
Mass (Da):101,836
Last modified:August 29, 2001 - v2
Checksum:iBE73686D7F79D3C9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti221N → D in CAA77642 (PubMed:1552854).Curated1
Sequence conflicti311 – 312SG → RR in CAA77642 (PubMed:1552854).Curated2
Sequence conflicti318N → H in CAA77642 (PubMed:1552854).Curated1
Sequence conflicti642T → S in CAA77642 (PubMed:1552854).Curated1
Sequence conflicti769V → VK (PubMed:1577255).Curated1
Sequence conflicti771 – 772GE → SM (PubMed:1577255).Curated2
Sequence conflicti903R → S in AAA21468 (PubMed:8005211).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11541 Genomic DNA Translation: CAA77642.1
X60439 Genomic DNA Translation: CAA42967.1
AF071034 Genomic DNA Translation: AAC31504.1
AF081182 Genomic DNA Translation: AAD05498.1
AF081183 Genomic DNA Translation: AAD05499.1
AE005174 Genomic DNA Translation: AAG58823.1
BA000007 Genomic DNA Translation: BAB37982.1
U32312 Genomic DNA Translation: AAB00111.1
L08095 Genomic DNA Translation: AAA21468.1
PIRiC86045
G91198
I41193
RefSeqiNP_312586.1, NC_002695.1
WP_000627885.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG58823; AAG58823; Z5110
BAB37982; BAB37982; BAB37982
GeneIDi915471
KEGGiece:Z5110
ecs:ECs4559
PATRICifig|386585.9.peg.4776

Similar proteinsi

Entry informationi

Entry nameiEAE_ECO57
AccessioniPrimary (citable) accession number: P43261
Secondary accession number(s): O85627, Q47168
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 29, 2001
Last modified: April 25, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health