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Protein

Intimin

Gene

eae

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for the production of attaching and effacing lesions on tissue culture cells.

GO - Biological processi

  • cell adhesion Source: InterPro
  • pathogenesis Source: CACAO
Complete GO annotation...

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciECOO157:EAE-MONOMER.

Protein family/group databases

TCDBi1.B.54.1.1. the intimin/invasin (int/inv) or autotransporter-3 (at-3) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Intimin
Alternative name(s):
Attaching and effacing protein
Short name:
Eae protein
Gamma-intimin
Gene namesi
Name:eae
Synonyms:eaeA
Ordered Locus Names:Z5110, ECs4559
ORF Names:L0025
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei20 – 39HelicalSequence analysisAdd BLAST20

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002118281 – 934IntiminAdd BLAST934

Interactioni

Protein-protein interaction databases

STRINGi155864.Z5110.

Structurei

Secondary structure

1934
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi212 – 221Combined sources10
Beta strandi225 – 236Combined sources12
Beta strandi238 – 251Combined sources14
Beta strandi254 – 266Combined sources13
Beta strandi271 – 281Combined sources11
Turni282 – 285Combined sources4
Beta strandi286 – 297Combined sources12
Beta strandi300 – 309Combined sources10
Beta strandi317 – 319Combined sources3
Beta strandi323 – 326Combined sources4
Beta strandi329 – 338Combined sources10
Beta strandi341 – 353Combined sources13
Beta strandi355 – 358Combined sources4
Beta strandi363 – 365Combined sources3
Beta strandi367 – 369Combined sources3
Beta strandi371 – 382Combined sources12
Beta strandi385 – 394Combined sources10
Helixi395 – 397Combined sources3
Beta strandi398 – 410Combined sources13
Helixi415 – 418Combined sources4
Helixi423 – 428Combined sources6
Turni430 – 432Combined sources3
Beta strandi446 – 448Combined sources3
Beta strandi760 – 764Combined sources5
Turni765 – 768Combined sources4
Beta strandi769 – 772Combined sources4
Beta strandi775 – 778Combined sources4
Beta strandi782 – 784Combined sources3
Beta strandi787 – 789Combined sources3
Beta strandi794 – 799Combined sources6
Turni801 – 803Combined sources3
Beta strandi804 – 806Combined sources3
Beta strandi810 – 814Combined sources5
Beta strandi816 – 818Combined sources3
Beta strandi820 – 826Combined sources7
Turni827 – 829Combined sources3
Beta strandi831 – 836Combined sources6
Beta strandi842 – 848Combined sources7
Helixi851 – 857Combined sources7
Turni858 – 860Combined sources3
Helixi866 – 876Combined sources11
Helixi879 – 881Combined sources3
Helixi883 – 885Combined sources3
Beta strandi892 – 894Combined sources3
Helixi899 – 904Combined sources6
Beta strandi906 – 911Combined sources6
Turni912 – 914Combined sources3
Beta strandi917 – 922Combined sources6
Beta strandi929 – 933Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZQKX-ray2.80A/B747-934[»]
2ZWKX-ray3.10A/C/E752-934[»]
3NCWX-ray2.80A/B/C/D747-934[»]
3NCXX-ray2.60A/B747-934[»]
4E1SX-ray1.86A208-449[»]
ProteinModelPortaliP43261.
SMRiP43261.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43261.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 112LysMPROSITE-ProRule annotationAdd BLAST50
Domaini560 – 653Big-1 1PROSITE-ProRule annotationAdd BLAST94
Domaini660 – 753Big-1 2PROSITE-ProRule annotationAdd BLAST94

Sequence similaritiesi

Belongs to the intimin/invasin family.Curated
Contains 1 LysM domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105TNG. Bacteria.
ENOG410XQE6. LUCA.
HOGENOMiHOG000217915.
KOiK12790.
OMAiPENMLGY.

Family and domain databases

Gene3Di2.60.40.920. 2 hits.
3.10.100.10. 1 hit.
InterProiIPR003344. Big_1_dom.
IPR003343. Big_2.
IPR016186. C-type_lectin-like/link.
IPR016187. CTDL_fold.
IPR024519. IAT_beta.
IPR003535. Intimin/invasin_bac.
IPR013117. Intimin_C.
IPR008964. Invasin/intimin_cell_adhesion.
IPR018392. LysM_dom.
[Graphical view]
PfamiPF02369. Big_1. 2 hits.
PF02368. Big_2. 1 hit.
PF11924. IAT_beta. 1 hit.
PF07979. Intimin_C. 1 hit.
PF01476. LysM. 1 hit.
[Graphical view]
PRINTSiPR01369. INTIMIN.
SMARTiSM00634. BID_1. 2 hits.
SM00635. BID_2. 1 hit.
SM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF49373. SSF49373. 3 hits.
SSF56436. SSF56436. 1 hit.
PROSITEiPS51127. BIG1. 2 hits.
PS51782. LYSM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITHGCYTRT RHKHKLKKTL IMLSAGLGLF FYVNQNSFAN GENYFKLGSD
60 70 80 90 100
SKLLTHDSYQ NRLFYTLKTG ETVADLSKSQ DINLSTIWSL NKHLYSSESE
110 120 130 140 150
MMKAAPGQQI ILPLKKLPFE YSALPLLGSA PLVAAGGVAG HTNKLTKMSP
160 170 180 190 200
DVTKSNMTDD KALNYAAQQA ASLGSQLQSR SLNGDYAKDT ALGIAGNQAS
210 220 230 240 250
SQLQAWLQHY GTAEVNLQSG NNFDGSSLDF LLPFYDSEKM LAFGQVGARY
260 270 280 290 300
IDSRFTANLG AGQRFFLPAN MLGYNVFIDQ DFSGDNTRLG IGGEYWRDYF
310 320 330 340 350
KSSVNGYFRM SGWHESYNKK DYDERPANGF DIRFNGYLPS YPALGAKLIY
360 370 380 390 400
EQYYGDNVAL FNSDKLQSNP GAATVGVNYT PIPLVTMGID YRHGTGNEND
410 420 430 440 450
LLYSMQFRYQ FDKSWSQQIE PQYVNELRTL SGSRYDLVQR NNNIILEYKK
460 470 480 490 500
QDILSLNIPH DINGTEHSTQ KIQLIVKSKY GLDRIVWDDS ALRSQGGQIQ
510 520 530 540 550
HSGSQSAQDY QAILPAYVQG GSNIYKVTAR AYDRNGNSSN NVQLTITVLS
560 570 580 590 600
NGQVVDQVGV TDFTADKTSA KADNADTITY TATVKKNGVA QANVPVSFNI
610 620 630 640 650
VSGTATLGAN SAKTDANGKA TVTLKSSTPG QVVVSAKTAE MTSALNASAV
660 670 680 690 700
IFFDQTKASI TEIKADKTTA VANGKDAIKY TVKVMKNGQP VNNQSVTFST
710 720 730 740 750
NFGMFNGKSQ TQATTGNDGR ATITLTSSSA GKATVSATVS DGAEVKATEV
760 770 780 790 800
TFFDELKIDN KVDIIGNNVR GELPNIWLQY GQFKLKASGG DGTYSWYSEN
810 820 830 840 850
TSIATVDASG KVTLNGKGSV VIKATSGDKQ TVSYTIKAPS YMIKVDKQAY
860 870 880 890 900
YADAMSICKN LLPSTQTVLS DIYDSWGAAN KYSHYSSMNS ITAWIKQTSS
910 920 930
EQRSGVSSTY NLITQNPLPG VNVNTPNVYA VCVE
Length:934
Mass (Da):101,836
Last modified:August 29, 2001 - v2
Checksum:iBE73686D7F79D3C9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti221N → D in CAA77642 (PubMed:1552854).Curated1
Sequence conflicti311 – 312SG → RR in CAA77642 (PubMed:1552854).Curated2
Sequence conflicti318N → H in CAA77642 (PubMed:1552854).Curated1
Sequence conflicti642T → S in CAA77642 (PubMed:1552854).Curated1
Sequence conflicti769V → VK (PubMed:1577255).Curated1
Sequence conflicti771 – 772GE → SM (PubMed:1577255).Curated2
Sequence conflicti903R → S in AAA21468 (PubMed:8005211).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11541 Genomic DNA. Translation: CAA77642.1.
X60439 Genomic DNA. Translation: CAA42967.1.
AF071034 Genomic DNA. Translation: AAC31504.1.
AF081182 Genomic DNA. Translation: AAD05498.1.
AF081183 Genomic DNA. Translation: AAD05499.1.
AE005174 Genomic DNA. Translation: AAG58823.1.
BA000007 Genomic DNA. Translation: BAB37982.1.
U32312 Genomic DNA. Translation: AAB00111.1.
L08095 Genomic DNA. Translation: AAA21468.1.
PIRiC86045.
G91198.
I41193.
RefSeqiNP_312586.1. NC_002695.1.
WP_000627885.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG58823; AAG58823; Z5110.
BAB37982; BAB37982; BAB37982.
GeneIDi915471.
KEGGiece:Z5110.
ecs:ECs4559.
PATRICi18358741. VBIEscCol44059_4535.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11541 Genomic DNA. Translation: CAA77642.1.
X60439 Genomic DNA. Translation: CAA42967.1.
AF071034 Genomic DNA. Translation: AAC31504.1.
AF081182 Genomic DNA. Translation: AAD05498.1.
AF081183 Genomic DNA. Translation: AAD05499.1.
AE005174 Genomic DNA. Translation: AAG58823.1.
BA000007 Genomic DNA. Translation: BAB37982.1.
U32312 Genomic DNA. Translation: AAB00111.1.
L08095 Genomic DNA. Translation: AAA21468.1.
PIRiC86045.
G91198.
I41193.
RefSeqiNP_312586.1. NC_002695.1.
WP_000627885.1. NZ_LPWC02000002.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZQKX-ray2.80A/B747-934[»]
2ZWKX-ray3.10A/C/E752-934[»]
3NCWX-ray2.80A/B/C/D747-934[»]
3NCXX-ray2.60A/B747-934[»]
4E1SX-ray1.86A208-449[»]
ProteinModelPortaliP43261.
SMRiP43261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z5110.

Protein family/group databases

TCDBi1.B.54.1.1. the intimin/invasin (int/inv) or autotransporter-3 (at-3) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG58823; AAG58823; Z5110.
BAB37982; BAB37982; BAB37982.
GeneIDi915471.
KEGGiece:Z5110.
ecs:ECs4559.
PATRICi18358741. VBIEscCol44059_4535.

Phylogenomic databases

eggNOGiENOG4105TNG. Bacteria.
ENOG410XQE6. LUCA.
HOGENOMiHOG000217915.
KOiK12790.
OMAiPENMLGY.

Enzyme and pathway databases

BioCyciECOO157:EAE-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP43261.

Family and domain databases

Gene3Di2.60.40.920. 2 hits.
3.10.100.10. 1 hit.
InterProiIPR003344. Big_1_dom.
IPR003343. Big_2.
IPR016186. C-type_lectin-like/link.
IPR016187. CTDL_fold.
IPR024519. IAT_beta.
IPR003535. Intimin/invasin_bac.
IPR013117. Intimin_C.
IPR008964. Invasin/intimin_cell_adhesion.
IPR018392. LysM_dom.
[Graphical view]
PfamiPF02369. Big_1. 2 hits.
PF02368. Big_2. 1 hit.
PF11924. IAT_beta. 1 hit.
PF07979. Intimin_C. 1 hit.
PF01476. LysM. 1 hit.
[Graphical view]
PRINTSiPR01369. INTIMIN.
SMARTiSM00634. BID_1. 2 hits.
SM00635. BID_2. 1 hit.
SM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF49373. SSF49373. 3 hits.
SSF56436. SSF56436. 1 hit.
PROSITEiPS51127. BIG1. 2 hits.
PS51782. LYSM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEAE_ECO57
AccessioniPrimary (citable) accession number: P43261
Secondary accession number(s): O85627, Q47168
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 29, 2001
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.