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Reviewed, UniProtKB/Swiss-Prot P43256 (HMDH2_ARATH)

Last modified November 25, 2008. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase 2
      Short name=HMG-CoA reductase 2
      Short name=HMGR2
    EC=1.1.1.34
Gene names
Name: HMG2
Ordered Locus Names: At2g17370
ORF Names: F5J6.24
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length562 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.

Catalytic activity

(R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.

Pathway

Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Tissue specificity

Restricted to young seedlings, roots, and inflorescences.

Sequence similarities

Belongs to the HMG-CoA reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5625623-hydroxy-3-methylglutaryl-coenzyme A reductase 2
PRO_0000114434

Regions

Transmembrane32 – 5625 Potential
Transmembrane77 – 10024 Potential
Region101 – 14646Linker By similarity
Region147 – 562416Catalytic By similarity

Sites

Active site2401Charge relay system By similarity
Active site3721Charge relay system By similarity
Active site4481Charge relay system By similarity
Active site5441Proton donor By similarity

Amino acid modifications

Glycosylation1181N-linked (GlcNAc...) Potential
Glycosylation3041N-linked (GlcNAc...) Potential
Glycosylation5481N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P43256-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: A4BF6BE6796C940A

FASTA56260,715
        10         20         30         40         50         60 
MEDLRRRFPT KKNGEEISNV AVDPPLRKAS DALPLPLYLT NTFFLSLFFA TVYFLLSRWR 

        70         80         90        100        110        120 
EKIRNSTPLH VVDLSEICAL IGFVASFIYL LGFCGIDLIF RSSSDDDVWV NDGMIPCNQS 

       130        140        150        160        170        180 
LDCREVLPIK PNSVDPPRES ELDSVEDEEI VKLVIDGTIP SYSLETKLGD CKRAAAIRRE 

       190        200        210        220        230        240 
AVQRITGKSL TGLPLEGFDY NSILGQCCEM PVGYVQIPVG IAGPLLLDGV EYSVPMATTE 

       250        260        270        280        290        300 
GCLVASTNRG FKAIHLSGGA FSVLVKDAMT RAPVVRFPSA RRAALVMFYL QDPSNFERLS 

       310        320        330        340        350        360 
LIFNKSSRFA RLQSITCTIA GRNLYPRFAC STGDAMGMNM VSKGVQNVLD FVKSEFPDMD 

       370        380        390        400        410        420 
VIGISGNYCS DKKASAVNWI EGRGKHVVCE AFIKAEIVEK VLKTSVEALV ELNTLKNLVG 

       430        440        450        460        470        480 
SAMAGSLGGF NAHSSNIVSA VFIATGQDPA QNVESSHCMT MILPDGDDLH ISVSMPCIEV 

       490        500        510        520        530        540 
GTVGGGTQLA SQAACLNLLG VKGSNNEKPG SNAQQLARIV AGSVLAGELS LMSAIAAGQL 

       550        560 
VKSHMKYNRS SRDIGPSSQV NR 

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis thaliana contains two differentially expressed 3-hydroxy-3-methylglutaryl-CoA reductase genes, which encode microsomal forms of the enzyme."
Enjuto M., Balcells L., Campos N., Caelles C., Arro M., Boronat A.
Proc. Natl. Acad. Sci. U.S.A. 91:927-931(1994) [PubMed: 8302869] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.

Cross-references

Sequence databases

L19262 Genomic DNA. Translation: AAA67317.1.
AC002329 Genomic DNA. Translation: AAB86514.1.
AY057680 mRNA. Translation: AAL15311.1.
AY143930 mRNA. Translation: AAN28869.1.
PIRD84551.
RefSeqNP_179329.1.
UniGeneAt.432

3D structure databases

HSSPHSSP built from PDB template 1DQA based on UniProtKB P04035.
ModBaseSearch...

Genome annotation databases

GeneID816244.
GenomeReviewsGene locus AT2G17370 in contig CT485783_GR.
KEGGath:AT2G17370.
NMPDRfig|3702.1.peg.8729.

Organism-specific databases

TAIRAt2g17370.

Gene expression databases

ArrayExpressP43256.
GermOnlineAT2G17370. Arabidopsis thaliana.

Family and domain databases

InterProIPR002202. HMG_CoA_Rdtase_cat.
IPR004554. HMG_CoA_Rdtase_I_cat.
[Graphical view]
Gene3DG3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHERPTHR10572. HMG-CoA_red. 1 hit.
PfamPF00368. HMG-CoA_red. 1 hit.
[Graphical view]
PRINTSPR00071. HMGCOARDTASE.
TIGRFAMsTIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITEPS00066. HMG_COA_REDUCTASE_1. 1 hit.
PS00318. HMG_COA_REDUCTASE_2. 1 hit.
PS01192. HMG_COA_REDUCTASE_3. 1 hit.
PS50065. HMG_COA_REDUCTASE_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHMDH2_ARATH
AccessionPrimary (citable) accession number: P43256
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 25, 2008
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents