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Protein

Prostacyclin receptor

Gene

Ptgir

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for prostacyclin (prostaglandin I2 or PGI2). The activity of this receptor is mediated by G(s) proteins which activate adenylate cyclase.

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: UniProtKB-KW

GO - Biological processi

  1. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: BHF-UCL
  2. negative regulation of platelet-derived growth factor receptor signaling pathway Source: BHF-UCL
  3. negative regulation of smooth muscle cell proliferation Source: BHF-UCL
  4. positive regulation of cAMP biosynthetic process Source: BHF-UCL
  5. positive regulation of cAMP-mediated signaling Source: BHF-UCL
  6. response to lipopolysaccharide Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_230556. Prostanoid ligand receptors.
REACT_242871. G alpha (s) signalling events.
REACT_262579. Prostacyclin signalling through prostacyclin receptor.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostacyclin receptor
Alternative name(s):
Prostaglandin I2 receptor
Short name:
PGI receptor
Short name:
PGI2 receptor
Prostanoid IP receptor
Gene namesi
Name:Ptgir
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:99535. Ptgir.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4444ExtracellularSequence AnalysisAdd
BLAST
Transmembranei45 – 6622Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini67 – 7913CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei80 – 10425Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini105 – 12218ExtracellularSequence AnalysisAdd
BLAST
Transmembranei123 – 14321Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini144 – 16219CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei163 – 18624Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini187 – 21529ExtracellularSequence AnalysisAdd
BLAST
Transmembranei216 – 23621Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini237 – 26327CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei264 – 28825Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini289 – 30113ExtracellularSequence AnalysisAdd
BLAST
Transmembranei302 – 32221Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini323 – 41593CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytosol Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi412 – 4121C → S: Abolishes isoprenylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412Prostacyclin receptorPRO_0000070076Add
BLAST
Propeptidei413 – 4153Removed in mature formSequence AnalysisPRO_0000240005

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 193PROSITE-ProRule annotation
Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi120 ↔ 198PROSITE-ProRule annotation
Lipidationi335 – 3351S-palmitoyl cysteineBy similarity
Lipidationi338 – 3381S-palmitoyl cysteineBy similarity
Modified residuei412 – 4121Cysteine methyl esterCurated
Lipidationi412 – 4121S-farnesyl cysteine1 Publication

Post-translational modificationi

Isoprenylation does not influence ligand binding but is required for efficient coupling to the effectors adenylyl cyclase and phospholipase C.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

MaxQBiP43252.
PaxDbiP43252.
PRIDEiP43252.

PTM databases

PhosphoSiteiP43252.

Expressioni

Gene expression databases

BgeeiP43252.
CleanExiMM_PTGIR.
ExpressionAtlasiP43252. baseline and differential.
GenevestigatoriP43252.

Interactioni

Subunit structurei

Interacts (non-isoprenylated C-terminus) with PDZK1.1 Publication

Protein-protein interaction databases

BioGridi202460. 4 interactions.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi411 – 4155Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4F8KX-ray1.70A/B409-415[»]
ProteinModelPortaliP43252.
SMRiP43252. Positions 300-330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG305292.
GeneTreeiENSGT00760000119188.
HOVERGENiHBG003074.
InParanoidiP43252.
KOiK04263.
OMAiCFLSPAV.
TreeFamiTF324982.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR008365. Prostanoid_rcpt.
IPR000370. Prostglndn_IP_rcpt.
[Graphical view]
PANTHERiPTHR11866. PTHR11866. 1 hit.
PTHR11866:SF7. PTHR11866:SF7. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01788. PROSTANOIDR.
PR00856. PRSTNOIDIPR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43252-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMASDGHPGP PSVTPGSPLS AGGREWQGMA GSCWNITYVQ DSVGPATSTL
60 70 80 90 100
MFVAGVVGNG LALGILGARR RSHPSAFAVL VTGLAVTDLL GTCFLSPAVF
110 120 130 140 150
VAYARNSSLL GLAHGGTMLC DTFAFAMTFF GLASTLILFA MAVERCLALS
160 170 180 190 200
HPYLYAQLDG PRCARFALPS IYAFCCLFCS LPLLGLGEHQ QYCPGSWCFI
210 220 230 240 250
RMRSAQPGGC AFSLAYASLM ALLVTSIFFC NGSVTLSLYH MYRQQRRHHG
260 270 280 290 300
SFVPTSRARE DEVYHLILLA LMTVIMAVCS LPLMIRGFTQ AIAPDSREMG
310 320 330 340 350
DLLAFRFNAF NPILDPWVFI LFRKAVFQRL KFWLCCLCAR SVHGDLQAPL
360 370 380 390 400
SRPASGRRDP PAPTSLQAKE GSWVPLSSWG TGQVAPLTAV PLTGGDGCSV
410
GMPSKSEAIA ACSLC
Length:415
Mass (Da):44,463
Last modified:November 1, 1995 - v1
Checksum:i306929582DDDD24C
GO

Sequence cautioni

The sequence BAA05144.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26157 mRNA. Translation: BAA05144.1. Different initiation.
BC094386 mRNA. Translation: AAH94386.1.
CCDSiCCDS20857.2.
PIRiA54416.
RefSeqiNP_032993.2. NM_008967.3.
UniGeneiMm.287572.

Genome annotation databases

EnsembliENSMUST00000144408; ENSMUSP00000122080; ENSMUSG00000043017.
GeneIDi19222.
KEGGimmu:19222.
UCSCiuc009fim.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26157 mRNA. Translation: BAA05144.1. Different initiation.
BC094386 mRNA. Translation: AAH94386.1.
CCDSiCCDS20857.2.
PIRiA54416.
RefSeqiNP_032993.2. NM_008967.3.
UniGeneiMm.287572.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4F8KX-ray1.70A/B409-415[»]
ProteinModelPortaliP43252.
SMRiP43252. Positions 300-330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202460. 4 interactions.

Chemistry

GuidetoPHARMACOLOGYi345.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP43252.

Proteomic databases

MaxQBiP43252.
PaxDbiP43252.
PRIDEiP43252.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000144408; ENSMUSP00000122080; ENSMUSG00000043017.
GeneIDi19222.
KEGGimmu:19222.
UCSCiuc009fim.2. mouse.

Organism-specific databases

CTDi5739.
MGIiMGI:99535. Ptgir.

Phylogenomic databases

eggNOGiNOG305292.
GeneTreeiENSGT00760000119188.
HOVERGENiHBG003074.
InParanoidiP43252.
KOiK04263.
OMAiCFLSPAV.
TreeFamiTF324982.

Enzyme and pathway databases

ReactomeiREACT_230556. Prostanoid ligand receptors.
REACT_242871. G alpha (s) signalling events.
REACT_262579. Prostacyclin signalling through prostacyclin receptor.

Miscellaneous databases

NextBioi296000.
PROiP43252.
SOURCEiSearch...

Gene expression databases

BgeeiP43252.
CleanExiMM_PTGIR.
ExpressionAtlasiP43252. baseline and differential.
GenevestigatoriP43252.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR008365. Prostanoid_rcpt.
IPR000370. Prostglndn_IP_rcpt.
[Graphical view]
PANTHERiPTHR11866. PTHR11866. 1 hit.
PTHR11866:SF7. PTHR11866:SF7. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01788. PROSTANOIDR.
PR00856. PRSTNOIDIPR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning of a mouse prostacyclin receptor. Multiple signaling pathways and expression in thymic medulla."
    Namba T., Oida H., Sugimoto Y., Negishi M., Kakizuka A., Ichikawa A., Narumiya S.
    J. Biol. Chem. 269:9986-9992(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Eye.
  3. "The prostacyclin receptor is isoprenylated. Isoprenylation is required for efficient receptor-effector coupling."
    Hayes J.S., Lawler O.A., Walsh M.T., Kinsella B.T.
    J. Biol. Chem. 274:23707-23718(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ISOPRENYLATION AT CYS-412, MUTAGENESIS OF CYS-412.
  4. "Molecular analysis of the prostacyclin receptor's interaction with the PDZ1 domain of its adaptor protein PDZK1."
    Birrane G., Mulvaney E.P., Pal R., Kinsella B.T., Kocher O.
    PLoS ONE 8:E53819-E53819(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 409-415 IN COMPLEX WITH PDZK1, INTERACTION WITH PDZK1.

Entry informationi

Entry nameiPI2R_MOUSE
AccessioniPrimary (citable) accession number: P43252
Secondary accession number(s): Q52KE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 4, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.