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P43252

- PI2R_MOUSE

UniProt

P43252 - PI2R_MOUSE

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Protein

Prostacyclin receptor

Gene
Ptgir
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Receptor for prostacyclin (prostaglandin I2 or PGI2). The activity of this receptor is mediated by G(s) proteins which activate adenylate cyclase.

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: UniProtKB-KW

GO - Biological processi

  1. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: BHF-UCL
  2. negative regulation of platelet-derived growth factor receptor signaling pathway Source: BHF-UCL
  3. negative regulation of smooth muscle cell proliferation Source: BHF-UCL
  4. positive regulation of cAMP biosynthetic process Source: BHF-UCL
  5. positive regulation of cAMP-mediated signaling Source: BHF-UCL
  6. response to lipopolysaccharide Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Prostacyclin receptor
Alternative name(s):
Prostaglandin I2 receptor
Short name:
PGI receptor
Short name:
PGI2 receptor
Prostanoid IP receptor
Gene namesi
Name:Ptgir
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:99535. Ptgir.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4444Extracellular Reviewed predictionAdd
BLAST
Transmembranei45 – 6622Helical; Name=1; Reviewed predictionAdd
BLAST
Topological domaini67 – 7913Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei80 – 10425Helical; Name=2; Reviewed predictionAdd
BLAST
Topological domaini105 – 12218Extracellular Reviewed predictionAdd
BLAST
Transmembranei123 – 14321Helical; Name=3; Reviewed predictionAdd
BLAST
Topological domaini144 – 16219Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei163 – 18624Helical; Name=4; Reviewed predictionAdd
BLAST
Topological domaini187 – 21529Extracellular Reviewed predictionAdd
BLAST
Transmembranei216 – 23621Helical; Name=5; Reviewed predictionAdd
BLAST
Topological domaini237 – 26327Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei264 – 28825Helical; Name=6; Reviewed predictionAdd
BLAST
Topological domaini289 – 30113Extracellular Reviewed predictionAdd
BLAST
Transmembranei302 – 32221Helical; Name=7; Reviewed predictionAdd
BLAST
Topological domaini323 – 41593Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi412 – 4121C → S: Abolishes isoprenylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412Prostacyclin receptorPRO_0000070076Add
BLAST
Propeptidei413 – 4153Removed in mature form Reviewed predictionPRO_0000240005

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 193 By similarity
Glycosylationi35 – 351N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi120 ↔ 198 By similarity
Lipidationi335 – 3351S-palmitoyl cysteine By similarity
Lipidationi338 – 3381S-palmitoyl cysteine By similarity
Modified residuei412 – 4121Cysteine methyl ester Inferred
Lipidationi412 – 4121S-farnesyl cysteine1 Publication

Post-translational modificationi

Isoprenylation does not influence ligand binding but is required for efficient coupling to the effectors adenylyl cyclase and phospholipase C.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

PaxDbiP43252.
PRIDEiP43252.

PTM databases

PhosphoSiteiP43252.

Expressioni

Gene expression databases

ArrayExpressiP43252.
BgeeiP43252.
CleanExiMM_PTGIR.
GenevestigatoriP43252.

Interactioni

Subunit structurei

Interacts (non-isoprenylated C-terminus) with PDZK1.1 Publication

Protein-protein interaction databases

BioGridi202460. 4 interactions.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi411 – 4155

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4F8KX-ray1.70A/B409-415[»]
ProteinModelPortaliP43252.
SMRiP43252. Positions 299-335.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG305292.
GeneTreeiENSGT00690000101953.
HOVERGENiHBG003074.
InParanoidiQ52KE5.
KOiK04263.
OMAiCFLSPAV.
TreeFamiTF324982.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR008365. Prostanoid_rcpt.
IPR000370. Prostglndn_IP_rcpt.
[Graphical view]
PANTHERiPTHR11866. PTHR11866. 1 hit.
PTHR11866:SF7. PTHR11866:SF7. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01788. PROSTANOIDR.
PR00856. PRSTNOIDIPR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43252-1 [UniParc]FASTAAdd to Basket

« Hide

MMASDGHPGP PSVTPGSPLS AGGREWQGMA GSCWNITYVQ DSVGPATSTL    50
MFVAGVVGNG LALGILGARR RSHPSAFAVL VTGLAVTDLL GTCFLSPAVF 100
VAYARNSSLL GLAHGGTMLC DTFAFAMTFF GLASTLILFA MAVERCLALS 150
HPYLYAQLDG PRCARFALPS IYAFCCLFCS LPLLGLGEHQ QYCPGSWCFI 200
RMRSAQPGGC AFSLAYASLM ALLVTSIFFC NGSVTLSLYH MYRQQRRHHG 250
SFVPTSRARE DEVYHLILLA LMTVIMAVCS LPLMIRGFTQ AIAPDSREMG 300
DLLAFRFNAF NPILDPWVFI LFRKAVFQRL KFWLCCLCAR SVHGDLQAPL 350
SRPASGRRDP PAPTSLQAKE GSWVPLSSWG TGQVAPLTAV PLTGGDGCSV 400
GMPSKSEAIA ACSLC 415
Length:415
Mass (Da):44,463
Last modified:November 1, 1995 - v1
Checksum:i306929582DDDD24C
GO

Sequence cautioni

The sequence BAA05144.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26157 mRNA. Translation: BAA05144.1. Different initiation.
BC094386 mRNA. Translation: AAH94386.1.
CCDSiCCDS20857.2.
PIRiA54416.
RefSeqiNP_032993.2. NM_008967.3.
UniGeneiMm.287572.

Genome annotation databases

EnsembliENSMUST00000144408; ENSMUSP00000122080; ENSMUSG00000043017.
GeneIDi19222.
KEGGimmu:19222.
UCSCiuc009fim.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26157 mRNA. Translation: BAA05144.1 . Different initiation.
BC094386 mRNA. Translation: AAH94386.1 .
CCDSi CCDS20857.2.
PIRi A54416.
RefSeqi NP_032993.2. NM_008967.3.
UniGenei Mm.287572.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4F8K X-ray 1.70 A/B 409-415 [» ]
ProteinModelPortali P43252.
SMRi P43252. Positions 299-335.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202460. 4 interactions.

Chemistry

BindingDBi P43252.
GuidetoPHARMACOLOGYi 345.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P43252.

Proteomic databases

PaxDbi P43252.
PRIDEi P43252.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000144408 ; ENSMUSP00000122080 ; ENSMUSG00000043017 .
GeneIDi 19222.
KEGGi mmu:19222.
UCSCi uc009fim.2. mouse.

Organism-specific databases

CTDi 5739.
MGIi MGI:99535. Ptgir.

Phylogenomic databases

eggNOGi NOG305292.
GeneTreei ENSGT00690000101953.
HOVERGENi HBG003074.
InParanoidi Q52KE5.
KOi K04263.
OMAi CFLSPAV.
TreeFami TF324982.

Miscellaneous databases

NextBioi 296000.
PROi P43252.
SOURCEi Search...

Gene expression databases

ArrayExpressi P43252.
Bgeei P43252.
CleanExi MM_PTGIR.
Genevestigatori P43252.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR008365. Prostanoid_rcpt.
IPR000370. Prostglndn_IP_rcpt.
[Graphical view ]
PANTHERi PTHR11866. PTHR11866. 1 hit.
PTHR11866:SF7. PTHR11866:SF7. 1 hit.
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR01788. PROSTANOIDR.
PR00856. PRSTNOIDIPR.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning of a mouse prostacyclin receptor. Multiple signaling pathways and expression in thymic medulla."
    Namba T., Oida H., Sugimoto Y., Negishi M., Kakizuka A., Ichikawa A., Narumiya S.
    J. Biol. Chem. 269:9986-9992(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Eye.
  3. "The prostacyclin receptor is isoprenylated. Isoprenylation is required for efficient receptor-effector coupling."
    Hayes J.S., Lawler O.A., Walsh M.T., Kinsella B.T.
    J. Biol. Chem. 274:23707-23718(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ISOPRENYLATION AT CYS-412, MUTAGENESIS OF CYS-412.
  4. "Molecular analysis of the prostacyclin receptor's interaction with the PDZ1 domain of its adaptor protein PDZK1."
    Birrane G., Mulvaney E.P., Pal R., Kinsella B.T., Kocher O.
    PLoS ONE 8:E53819-E53819(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 409-415 IN COMPLEX WITH PDZK1, INTERACTION WITH PDZK1.

Entry informationi

Entry nameiPI2R_MOUSE
AccessioniPrimary (citable) accession number: P43252
Secondary accession number(s): Q52KE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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