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P43252

- PI2R_MOUSE

UniProt

P43252 - PI2R_MOUSE

Protein

Prostacyclin receptor

Gene

Ptgir

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Receptor for prostacyclin (prostaglandin I2 or PGI2). The activity of this receptor is mediated by G(s) proteins which activate adenylate cyclase.

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: UniProtKB-KW

    GO - Biological processi

    1. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: BHF-UCL
    2. negative regulation of platelet-derived growth factor receptor signaling pathway Source: BHF-UCL
    3. negative regulation of smooth muscle cell proliferation Source: BHF-UCL
    4. positive regulation of cAMP biosynthetic process Source: BHF-UCL
    5. positive regulation of cAMP-mediated signaling Source: BHF-UCL
    6. response to lipopolysaccharide Source: MGI

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prostacyclin receptor
    Alternative name(s):
    Prostaglandin I2 receptor
    Short name:
    PGI receptor
    Short name:
    PGI2 receptor
    Prostanoid IP receptor
    Gene namesi
    Name:Ptgir
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:99535. Ptgir.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi412 – 4121C → S: Abolishes isoprenylation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 412412Prostacyclin receptorPRO_0000070076Add
    BLAST
    Propeptidei413 – 4153Removed in mature formSequence AnalysisPRO_0000240005

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi33 ↔ 193PROSITE-ProRule annotation
    Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi120 ↔ 198PROSITE-ProRule annotation
    Lipidationi335 – 3351S-palmitoyl cysteineBy similarity
    Lipidationi338 – 3381S-palmitoyl cysteineBy similarity
    Modified residuei412 – 4121Cysteine methyl esterCurated
    Lipidationi412 – 4121S-farnesyl cysteine1 Publication

    Post-translational modificationi

    Isoprenylation does not influence ligand binding but is required for efficient coupling to the effectors adenylyl cyclase and phospholipase C.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Methylation, Palmitate, Prenylation

    Proteomic databases

    PaxDbiP43252.
    PRIDEiP43252.

    PTM databases

    PhosphoSiteiP43252.

    Expressioni

    Gene expression databases

    ArrayExpressiP43252.
    BgeeiP43252.
    CleanExiMM_PTGIR.
    GenevestigatoriP43252.

    Interactioni

    Subunit structurei

    Interacts (non-isoprenylated C-terminus) with PDZK1.1 Publication

    Protein-protein interaction databases

    BioGridi202460. 4 interactions.

    Structurei

    Secondary structure

    1
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi411 – 4155

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4F8KX-ray1.70A/B409-415[»]
    ProteinModelPortaliP43252.
    SMRiP43252. Positions 299-335.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4444ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini67 – 7913CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini105 – 12218ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini144 – 16219CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini187 – 21529ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini237 – 26327CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini289 – 30113ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini323 – 41593CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei45 – 6622Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei80 – 10425Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei123 – 14321Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei163 – 18624Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei216 – 23621Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei264 – 28825Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei302 – 32221Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG305292.
    GeneTreeiENSGT00690000101953.
    HOVERGENiHBG003074.
    InParanoidiQ52KE5.
    KOiK04263.
    OMAiCFLSPAV.
    TreeFamiTF324982.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR008365. Prostanoid_rcpt.
    IPR000370. Prostglndn_IP_rcpt.
    [Graphical view]
    PANTHERiPTHR11866. PTHR11866. 1 hit.
    PTHR11866:SF7. PTHR11866:SF7. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR01788. PROSTANOIDR.
    PR00856. PRSTNOIDIPR.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43252-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMASDGHPGP PSVTPGSPLS AGGREWQGMA GSCWNITYVQ DSVGPATSTL    50
    MFVAGVVGNG LALGILGARR RSHPSAFAVL VTGLAVTDLL GTCFLSPAVF 100
    VAYARNSSLL GLAHGGTMLC DTFAFAMTFF GLASTLILFA MAVERCLALS 150
    HPYLYAQLDG PRCARFALPS IYAFCCLFCS LPLLGLGEHQ QYCPGSWCFI 200
    RMRSAQPGGC AFSLAYASLM ALLVTSIFFC NGSVTLSLYH MYRQQRRHHG 250
    SFVPTSRARE DEVYHLILLA LMTVIMAVCS LPLMIRGFTQ AIAPDSREMG 300
    DLLAFRFNAF NPILDPWVFI LFRKAVFQRL KFWLCCLCAR SVHGDLQAPL 350
    SRPASGRRDP PAPTSLQAKE GSWVPLSSWG TGQVAPLTAV PLTGGDGCSV 400
    GMPSKSEAIA ACSLC 415
    Length:415
    Mass (Da):44,463
    Last modified:November 1, 1995 - v1
    Checksum:i306929582DDDD24C
    GO

    Sequence cautioni

    The sequence BAA05144.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26157 mRNA. Translation: BAA05144.1. Different initiation.
    BC094386 mRNA. Translation: AAH94386.1.
    CCDSiCCDS20857.2.
    PIRiA54416.
    RefSeqiNP_032993.2. NM_008967.3.
    UniGeneiMm.287572.

    Genome annotation databases

    EnsembliENSMUST00000144408; ENSMUSP00000122080; ENSMUSG00000043017.
    GeneIDi19222.
    KEGGimmu:19222.
    UCSCiuc009fim.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26157 mRNA. Translation: BAA05144.1 . Different initiation.
    BC094386 mRNA. Translation: AAH94386.1 .
    CCDSi CCDS20857.2.
    PIRi A54416.
    RefSeqi NP_032993.2. NM_008967.3.
    UniGenei Mm.287572.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4F8K X-ray 1.70 A/B 409-415 [» ]
    ProteinModelPortali P43252.
    SMRi P43252. Positions 299-335.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202460. 4 interactions.

    Chemistry

    BindingDBi P43252.
    GuidetoPHARMACOLOGYi 345.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P43252.

    Proteomic databases

    PaxDbi P43252.
    PRIDEi P43252.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000144408 ; ENSMUSP00000122080 ; ENSMUSG00000043017 .
    GeneIDi 19222.
    KEGGi mmu:19222.
    UCSCi uc009fim.2. mouse.

    Organism-specific databases

    CTDi 5739.
    MGIi MGI:99535. Ptgir.

    Phylogenomic databases

    eggNOGi NOG305292.
    GeneTreei ENSGT00690000101953.
    HOVERGENi HBG003074.
    InParanoidi Q52KE5.
    KOi K04263.
    OMAi CFLSPAV.
    TreeFami TF324982.

    Miscellaneous databases

    NextBioi 296000.
    PROi P43252.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P43252.
    Bgeei P43252.
    CleanExi MM_PTGIR.
    Genevestigatori P43252.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR008365. Prostanoid_rcpt.
    IPR000370. Prostglndn_IP_rcpt.
    [Graphical view ]
    PANTHERi PTHR11866. PTHR11866. 1 hit.
    PTHR11866:SF7. PTHR11866:SF7. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01788. PROSTANOIDR.
    PR00856. PRSTNOIDIPR.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning of a mouse prostacyclin receptor. Multiple signaling pathways and expression in thymic medulla."
      Namba T., Oida H., Sugimoto Y., Negishi M., Kakizuka A., Ichikawa A., Narumiya S.
      J. Biol. Chem. 269:9986-9992(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Eye.
    3. "The prostacyclin receptor is isoprenylated. Isoprenylation is required for efficient receptor-effector coupling."
      Hayes J.S., Lawler O.A., Walsh M.T., Kinsella B.T.
      J. Biol. Chem. 274:23707-23718(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: ISOPRENYLATION AT CYS-412, MUTAGENESIS OF CYS-412.
    4. "Molecular analysis of the prostacyclin receptor's interaction with the PDZ1 domain of its adaptor protein PDZK1."
      Birrane G., Mulvaney E.P., Pal R., Kinsella B.T., Kocher O.
      PLoS ONE 8:E53819-E53819(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 409-415 IN COMPLEX WITH PDZK1, INTERACTION WITH PDZK1.

    Entry informationi

    Entry nameiPI2R_MOUSE
    AccessioniPrimary (citable) accession number: P43252
    Secondary accession number(s): Q52KE5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3