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Protein

Prostacyclin receptor

Gene

Ptgir

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for prostacyclin (prostaglandin I2 or PGI2). The activity of this receptor is mediated by G(s) proteins which activate adenylate cyclase.

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: BHF-UCL
  • cellular response to prostaglandin D stimulus Source: GO_Central
  • inflammatory response Source: GO_Central
  • negative regulation of platelet-derived growth factor receptor signaling pathway Source: BHF-UCL
  • negative regulation of smooth muscle cell proliferation Source: BHF-UCL
  • positive regulation of cAMP biosynthetic process Source: BHF-UCL
  • positive regulation of cAMP-mediated signaling Source: BHF-UCL
  • positive regulation of cytosolic calcium ion concentration Source: GO_Central
  • response to lipopolysaccharide Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-391908. Prostanoid ligand receptors.
R-MMU-392851. Prostacyclin signalling through prostacyclin receptor.
R-MMU-418555. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostacyclin receptor
Alternative name(s):
Prostaglandin I2 receptor
Short name:
PGI receptor
Short name:
PGI2 receptor
Prostanoid IP receptor
Gene namesi
Name:Ptgir
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:99535. Ptgir.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 44ExtracellularSequence analysisAdd BLAST44
Transmembranei45 – 66Helical; Name=1Sequence analysisAdd BLAST22
Topological domaini67 – 79CytoplasmicSequence analysisAdd BLAST13
Transmembranei80 – 104Helical; Name=2Sequence analysisAdd BLAST25
Topological domaini105 – 122ExtracellularSequence analysisAdd BLAST18
Transmembranei123 – 143Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini144 – 162CytoplasmicSequence analysisAdd BLAST19
Transmembranei163 – 186Helical; Name=4Sequence analysisAdd BLAST24
Topological domaini187 – 215ExtracellularSequence analysisAdd BLAST29
Transmembranei216 – 236Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini237 – 263CytoplasmicSequence analysisAdd BLAST27
Transmembranei264 – 288Helical; Name=6Sequence analysisAdd BLAST25
Topological domaini289 – 301ExtracellularSequence analysisAdd BLAST13
Transmembranei302 – 322Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini323 – 415CytoplasmicSequence analysisAdd BLAST93

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi412C → S: Abolishes isoprenylation. 1 Publication1

Chemistry databases

GuidetoPHARMACOLOGYi345.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000700761 – 412Prostacyclin receptorAdd BLAST412
PropeptideiPRO_0000240005413 – 415Removed in mature formSequence analysis3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 193PROSITE-ProRule annotation
Glycosylationi35N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi120 ↔ 198PROSITE-ProRule annotation
Lipidationi335S-palmitoyl cysteineBy similarity1
Lipidationi338S-palmitoyl cysteineBy similarity1
Modified residuei365PhosphoserineBy similarity1
Modified residuei412Cysteine methyl esterCurated1
Lipidationi412S-farnesyl cysteine1 Publication1

Post-translational modificationi

Isoprenylation does not influence ligand binding but is required for efficient coupling to the effectors adenylyl cyclase and phospholipase C.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Methylation, Palmitate, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiP43252.
PaxDbiP43252.
PRIDEiP43252.

PTM databases

iPTMnetiP43252.
PhosphoSitePlusiP43252.

Expressioni

Gene expression databases

BgeeiENSMUSG00000043017.
CleanExiMM_PTGIR.
ExpressionAtlasiP43252. baseline and differential.
GenevisibleiP43252. MM.

Interactioni

Subunit structurei

Interacts (non-isoprenylated C-terminus) with PDZK1.1 Publication

Protein-protein interaction databases

BioGridi202460. 4 interactors.
STRINGi10090.ENSMUSP00000122080.

Structurei

Secondary structure

1415
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi411 – 415Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F8KX-ray1.70A/B409-415[»]
ProteinModelPortaliP43252.
SMRiP43252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000119188.
HOVERGENiHBG003074.
InParanoidiP43252.
KOiK04263.
OMAiFTQAIAP.
OrthoDBiEOG091G0F07.
TreeFamiTF324982.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR008365. Prostanoid_rcpt.
IPR000370. Prostglndn_IP_rcpt.
[Graphical view]
PANTHERiPTHR11866. PTHR11866. 1 hit.
PTHR11866:SF7. PTHR11866:SF7. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01788. PROSTANOIDR.
PR00856. PRSTNOIDIPR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43252-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMASDGHPGP PSVTPGSPLS AGGREWQGMA GSCWNITYVQ DSVGPATSTL
60 70 80 90 100
MFVAGVVGNG LALGILGARR RSHPSAFAVL VTGLAVTDLL GTCFLSPAVF
110 120 130 140 150
VAYARNSSLL GLAHGGTMLC DTFAFAMTFF GLASTLILFA MAVERCLALS
160 170 180 190 200
HPYLYAQLDG PRCARFALPS IYAFCCLFCS LPLLGLGEHQ QYCPGSWCFI
210 220 230 240 250
RMRSAQPGGC AFSLAYASLM ALLVTSIFFC NGSVTLSLYH MYRQQRRHHG
260 270 280 290 300
SFVPTSRARE DEVYHLILLA LMTVIMAVCS LPLMIRGFTQ AIAPDSREMG
310 320 330 340 350
DLLAFRFNAF NPILDPWVFI LFRKAVFQRL KFWLCCLCAR SVHGDLQAPL
360 370 380 390 400
SRPASGRRDP PAPTSLQAKE GSWVPLSSWG TGQVAPLTAV PLTGGDGCSV
410
GMPSKSEAIA ACSLC
Length:415
Mass (Da):44,463
Last modified:November 1, 1995 - v1
Checksum:i306929582DDDD24C
GO

Sequence cautioni

The sequence BAA05144 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26157 mRNA. Translation: BAA05144.1. Different initiation.
BC094386 mRNA. Translation: AAH94386.1.
CCDSiCCDS20857.2.
PIRiA54416.
RefSeqiNP_032993.2. NM_008967.3.
UniGeneiMm.287572.

Genome annotation databases

EnsembliENSMUST00000144408; ENSMUSP00000122080; ENSMUSG00000043017.
GeneIDi19222.
KEGGimmu:19222.
UCSCiuc009fim.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26157 mRNA. Translation: BAA05144.1. Different initiation.
BC094386 mRNA. Translation: AAH94386.1.
CCDSiCCDS20857.2.
PIRiA54416.
RefSeqiNP_032993.2. NM_008967.3.
UniGeneiMm.287572.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F8KX-ray1.70A/B409-415[»]
ProteinModelPortaliP43252.
SMRiP43252.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202460. 4 interactors.
STRINGi10090.ENSMUSP00000122080.

Chemistry databases

GuidetoPHARMACOLOGYi345.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP43252.
PhosphoSitePlusiP43252.

Proteomic databases

MaxQBiP43252.
PaxDbiP43252.
PRIDEiP43252.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000144408; ENSMUSP00000122080; ENSMUSG00000043017.
GeneIDi19222.
KEGGimmu:19222.
UCSCiuc009fim.2. mouse.

Organism-specific databases

CTDi5739.
MGIiMGI:99535. Ptgir.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000119188.
HOVERGENiHBG003074.
InParanoidiP43252.
KOiK04263.
OMAiFTQAIAP.
OrthoDBiEOG091G0F07.
TreeFamiTF324982.

Enzyme and pathway databases

ReactomeiR-MMU-391908. Prostanoid ligand receptors.
R-MMU-392851. Prostacyclin signalling through prostacyclin receptor.
R-MMU-418555. G alpha (s) signalling events.

Miscellaneous databases

PROiP43252.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000043017.
CleanExiMM_PTGIR.
ExpressionAtlasiP43252. baseline and differential.
GenevisibleiP43252. MM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR008365. Prostanoid_rcpt.
IPR000370. Prostglndn_IP_rcpt.
[Graphical view]
PANTHERiPTHR11866. PTHR11866. 1 hit.
PTHR11866:SF7. PTHR11866:SF7. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01788. PROSTANOIDR.
PR00856. PRSTNOIDIPR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPI2R_MOUSE
AccessioniPrimary (citable) accession number: P43252
Secondary accession number(s): Q52KE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.