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P43252 (PI2R_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prostacyclin receptor
Alternative name(s):
Prostaglandin I2 receptor
Short name=PGI receptor
Short name=PGI2 receptor
Prostanoid IP receptor
Gene names
Name:Ptgir
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for prostacyclin (prostaglandin I2 or PGI2). The activity of this receptor is mediated by G(s) proteins which activate adenylate cyclase.

Subunit structure

Interacts (non-isoprenylated C-terminus) with PDZK1. Ref.4

Subcellular location

Cell membrane; Multi-pass membrane protein.

Post-translational modification

Isoprenylation does not influence ligand binding but is required for efficient coupling to the effectors adenylyl cyclase and phospholipase C.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence caution

The sequence BAA05144.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412Prostacyclin receptor
PRO_0000070076
Propeptide413 – 4153Removed in mature form Potential
PRO_0000240005

Regions

Topological domain1 – 4444Extracellular Potential
Transmembrane45 – 6622Helical; Name=1; Potential
Topological domain67 – 7913Cytoplasmic Potential
Transmembrane80 – 10425Helical; Name=2; Potential
Topological domain105 – 12218Extracellular Potential
Transmembrane123 – 14321Helical; Name=3; Potential
Topological domain144 – 16219Cytoplasmic Potential
Transmembrane163 – 18624Helical; Name=4; Potential
Topological domain187 – 21529Extracellular Potential
Transmembrane216 – 23621Helical; Name=5; Potential
Topological domain237 – 26327Cytoplasmic Potential
Transmembrane264 – 28825Helical; Name=6; Potential
Topological domain289 – 30113Extracellular Potential
Transmembrane302 – 32221Helical; Name=7; Potential
Topological domain323 – 41593Cytoplasmic Potential

Amino acid modifications

Modified residue4121Cysteine methyl ester Probable
Lipidation3351S-palmitoyl cysteine By similarity
Lipidation3381S-palmitoyl cysteine By similarity
Lipidation4121S-farnesyl cysteine Ref.3
Glycosylation351N-linked (GlcNAc...) Potential
Disulfide bond33 ↔ 193 By similarity
Disulfide bond120 ↔ 198 By similarity

Experimental info

Mutagenesis4121C → S: Abolishes isoprenylation. Ref.3

Secondary structure

.. 415
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P43252 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 306929582DDDD24C

FASTA41544,463
        10         20         30         40         50         60 
MMASDGHPGP PSVTPGSPLS AGGREWQGMA GSCWNITYVQ DSVGPATSTL MFVAGVVGNG 

        70         80         90        100        110        120 
LALGILGARR RSHPSAFAVL VTGLAVTDLL GTCFLSPAVF VAYARNSSLL GLAHGGTMLC 

       130        140        150        160        170        180 
DTFAFAMTFF GLASTLILFA MAVERCLALS HPYLYAQLDG PRCARFALPS IYAFCCLFCS 

       190        200        210        220        230        240 
LPLLGLGEHQ QYCPGSWCFI RMRSAQPGGC AFSLAYASLM ALLVTSIFFC NGSVTLSLYH 

       250        260        270        280        290        300 
MYRQQRRHHG SFVPTSRARE DEVYHLILLA LMTVIMAVCS LPLMIRGFTQ AIAPDSREMG 

       310        320        330        340        350        360 
DLLAFRFNAF NPILDPWVFI LFRKAVFQRL KFWLCCLCAR SVHGDLQAPL SRPASGRRDP 

       370        380        390        400        410 
PAPTSLQAKE GSWVPLSSWG TGQVAPLTAV PLTGGDGCSV GMPSKSEAIA ACSLC 

« Hide

References

« Hide 'large scale' references
[1]"cDNA cloning of a mouse prostacyclin receptor. Multiple signaling pathways and expression in thymic medulla."
Namba T., Oida H., Sugimoto Y., Negishi M., Kakizuka A., Ichikawa A., Narumiya S.
J. Biol. Chem. 269:9986-9992(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Eye.
[3]"The prostacyclin receptor is isoprenylated. Isoprenylation is required for efficient receptor-effector coupling."
Hayes J.S., Lawler O.A., Walsh M.T., Kinsella B.T.
J. Biol. Chem. 274:23707-23718(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: ISOPRENYLATION AT CYS-412, MUTAGENESIS OF CYS-412.
[4]"Molecular analysis of the prostacyclin receptor's interaction with the PDZ1 domain of its adaptor protein PDZK1."
Birrane G., Mulvaney E.P., Pal R., Kinsella B.T., Kocher O.
PLoS ONE 8:E53819-E53819(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 409-415 IN COMPLEX WITH PDZK1, INTERACTION WITH PDZK1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D26157 mRNA. Translation: BAA05144.1. Different initiation.
BC094386 mRNA. Translation: AAH94386.1.
CCDSCCDS20857.2.
PIRA54416.
RefSeqNP_032993.2. NM_008967.3.
UniGeneMm.287572.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4F8KX-ray1.70A/B409-415[»]
ProteinModelPortalP43252.
SMRP43252. Positions 299-335.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202460. 4 interactions.

Chemistry

BindingDBP43252.
GuidetoPHARMACOLOGY345.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP43252.

Proteomic databases

PaxDbP43252.
PRIDEP43252.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000144408; ENSMUSP00000122080; ENSMUSG00000043017.
GeneID19222.
KEGGmmu:19222.
UCSCuc009fim.2. mouse.

Organism-specific databases

CTD5739.
MGIMGI:99535. Ptgir.

Phylogenomic databases

eggNOGNOG305292.
GeneTreeENSGT00690000101953.
HOVERGENHBG003074.
InParanoidQ52KE5.
KOK04263.
OMACFLSPAV.
TreeFamTF324982.

Gene expression databases

ArrayExpressP43252.
BgeeP43252.
CleanExMM_PTGIR.
GenevestigatorP43252.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR008365. Prostanoid_rcpt.
IPR000370. Prostglndn_IP_rcpt.
[Graphical view]
PANTHERPTHR11866. PTHR11866. 1 hit.
PTHR11866:SF7. PTHR11866:SF7. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR01788. PROSTANOIDR.
PR00856. PRSTNOIDIPR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio296000.
PROP43252.
SOURCESearch...

Entry information

Entry namePI2R_MOUSE
AccessionPrimary (citable) accession number: P43252
Secondary accession number(s): Q52KE5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries