Reviewed, UniProtKB/Swiss-Prot P43249 (GRK5_BOVIN)

Names and origin

Protein names

Recommended name:
    G protein-coupled receptor kinase 5
    EC=2.7.11.16
Alternative name(s):
    G protein-coupled receptor kinase GRK5

Gene names

Name:	GRK5
Synonyms:	GPRK5

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically phosphorylates the activated forms of G protein-coupled receptors.
Catalytic activity	ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.
Tissue specificity	Highest levels in lung, heart, retina, lingual epithelium. Very little in brain, liver, kidney.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	G protein-coupled receptor kinase 5		PRO_0000085970
Regions
	Domain	53 – 171	119	RGS
	Domain	186 – 448	263	Protein kinase
	Domain	449 – 514	66	AGC-kinase C-terminal
	Nucleotide binding	192 – 200	9	ATP By similarity
	Region	1 – 185	185	N-terminal
Sites
	Active site	311	1	Proton acceptor By similarity
	Binding site	215	1	ATP By similarity
Amino acid modifications
	Modified residue	484	1	Phosphoserine; by autocatalysis By similarity
	Modified residue	485	1	Phosphothreonine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43249-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: E8F353697D8BA6E2	FASTA	590	67,889
	10 20 30 40 50 60 MELENIVANT VLLKAREGGG GKRKGKSKKW KEILKFPHIN QCEDLRRTID RDYCSLCDKQ 70 80 90 100 110 120 PVGRLLFRQF CETRPGLESY IQFLDSVAEY EVTPDEKLGE KGKEIMTKYL TPKSPVFITQ 130 140 150 160 170 180 VGRDLVSQTE EKLLQKPCKE LFSACVQSVH DYLRGEPFHE YLDSMYFDRF LQWKWLERQP 190 200 210 220 230 240 VTKNTFRQYR VLGKGGFGEV CACQVRATGK MYACKRLEKK RIKKRKGESM ALNEKQILEK 250 260 270 280 290 300 VNSRFVVNLA YAYETKDALC LVLTIMNGGD LKFHIYNMGN PGFEEERALF YAAEILCGLE 310 320 330 340 350 360 DLHHENIVYR DLKPENILLD DYGHIRISDL GLAVKIPEGD LIRGRVGTVG YMAPEVLNNQ 370 380 390 400 410 420 RYGLSPDYWG LGCLIYEMIE GQSPFRGRKE KVKREEVDRR VLETEEVYSH KFSEEAKSIC 430 440 450 460 470 480 KMLLTKDAKQ RLGCQEEGAA EVKRHPFFRN MNFKRLEAGM LDPPFVPDPR AVYCKDVLDI 490 500 510 520 530 540 EQFSTVKGVN LDHTDDDFYS KFSTGSVPIP WQSEMIETEC FKELNVFGPH GTLSPDLNRS 550 560 570 580 590 HPPEPPKKGL LQRLFKRQHQ NNSKSSPNSK TSFNHHINSN HVSSNSTGSS

References

[1]

"Identification, purification, and characterization of GRK5, a member of the family of G protein-coupled receptor kinases." Premont R.T., Koch W.J., Inglese J., Lefkowitz R.J. J. Biol. Chem. 269:6832-6841(1994) [PubMed: 8120045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Tongue epithelium.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U01206 mRNA. Translation: AAA17561.1.
IPI	IPI00698786.
PIR	A54372.
RefSeq	NP_776756.1.
UniGene	Bt.4263
3D structure databases
HSSP	HSSP built from PDB template 1GZK based on UniProtKB P31751.
SMR	P43249. Positions 23-532.
ModBase	Search...
Protein-protein interaction databases
STRING	P43249.
Genome annotation databases
Ensembl	ENSBTAT00000010492; ENSBTAP00000010492; ENSBTAG00000007981; Bos taurus. [Genome view]
GeneID	281801.
KEGG	bta:281801.
Organism-specific databases
CTD	281801.
Phylogenomic databases
HOVERGEN	P43249.
Enzyme and pathway databases
BRENDA	2.7.11.16. 251.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000239. GPCR_kinase. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRK5_BOVIN

Accession

Primary (citable) accession number: P43249

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 1, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)