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Protein

DNA mismatch repair protein spellchecker 1

Gene

spel1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in postreplication mismatch repair. Binds specifically to DNA containing mismatched nucleotides thus providing a target for the excision repair processes characteristic of postreplication mismatch repair (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi667 – 6748ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB
  • mismatched DNA binding Source: InterPro

GO - Biological processi

  • interstrand cross-link repair Source: FlyBase
  • maintenance of DNA repeat elements Source: FlyBase
  • mismatch repair Source: UniProtKB
  • postreplication repair Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein spellchecker 1
Gene namesi
Name:spel1
ORF Names:CG4215
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0015546. spel1.

Subcellular locationi

GO - Cellular componenti

  • mismatch repair complex Source: InterPro
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 917917DNA mismatch repair protein spellchecker 1PRO_0000115186Add
BLAST

Proteomic databases

PaxDbiP43248.

Expressioni

Gene expression databases

BgeeiP43248.
ExpressionAtlasiP43248. differential.
GenevisibleiP43248. DM.

Interactioni

Subunit structurei

Heterodimer of Msh2/Spel and Msh6.By similarity

Protein-protein interaction databases

BioGridi60866. 4 interactions.
DIPiDIP-21499N.
IntActiP43248. 2 interactions.
MINTiMINT-1547583.
STRINGi7227.FBpp0301208.

Structurei

3D structure databases

ProteinModelPortaliP43248.
SMRiP43248. Positions 13-851.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA mismatch repair MutS family.Curated

Phylogenomic databases

eggNOGiKOG0219. Eukaryota.
COG0249. LUCA.
GeneTreeiENSGT00550000074867.
InParanoidiP43248.
KOiK08735.
OMAiNDWYLAY.
OrthoDBiEOG76DTRW.
PhylomeDBiP43248.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR011184. DNA_mismatch_repair_MutS.
IPR007695. DNA_mismatch_repair_MutS-lik_N.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR032642. Msh2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11361:SF35. PTHR11361:SF35. 1 hit.
PfamiPF01624. MutS_I. 1 hit.
PF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
PIRSFiPIRSF005813. MSH2. 1 hit.
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43248-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAKATDSRQ EPTLNMDTNA RRNFIKFHAK LGEKPATTVR FFDHTDRYTV
60 70 80 90 100
HGSDDCELVA KIVYKSTAFI GALLPDDKKE TLQFVSMSKG NFELAVRELL
110 120 130 140 150
LVRNYRVEVY VKNSSDWEIE YRGSPGNLLQ FEDILFSNKE VLVGNSIISL
160 170 180 190 200
LVKLDGGGQR RVGVASVEQN DCKFQLLEFL DDDFFTELEA TVVLLGPKEC
210 220 230 240 250
LLPSIEGEYS AVKTLLDRNG VMITMPKKSG DNDLLQDLNR LLRFAKGQQE
260 270 280 290 300
DATGLKELQL QLASNALKTA IKYLDLVNDA GNLGHYEIKQ LDLNRFVHLD
310 320 330 340 350
SAAVAALNIM PKPGTHPSMP SYRWQSVLGV LDHCRTPQGH RLMGQWVKQP
360 370 380 390 400
LRSRNILNDR HNIVQCLLES PDTMETLSLD YLKRIPDILM LTKKLMRRKA
410 420 430 440 450
NLQDLFRIYQ VILRTPKILK VLHELDNSTI ESVICAPFKS FLKDLTGLKQ
460 470 480 490 500
MVEQVVDFEA IERGEYLVKA SFDSRLMELQ QMMTELYSKM EELQFKCSQE
510 520 530 540 550
LNLDGKNQVK LESVAKLGHH FRITVKDDSV LRKNKNYRIV DVIKGGVRFT
560 570 580 590 600
SDKLEGYADE FASCRTRYEE QQLSIVEEII HVAVGYAAPL TLLNNELAQL
610 620 630 640 650
DCLVSFAIAA RSAPTPYVRP KMLEEGAREL VLEDVRHPCL ELQEHVNFIA
660 670 680 690 700
NSVDFKKEEC NMFIITGPNM GGKSTYIRSV GTAVLMAHIG AFVPCSLATI
710 720 730 740 750
SMVDSILGRV GASDNIIKGL STFMVEMIET SGIIRTATDK SLVIIDELGR
760 770 780 790 800
GTSTYEGCGI AWSIAEHLAK ETKCFTLFAT HFHEITKLAE TLSTVKNCHM
810 820 830 840 850
AAVADADDFT LLYQVRSGVM EKSFGIQVAR LANFPEHVVQ NAQEVYNEFE
860 870 880 890 900
DEHVDKQKKE DKALLEKIQV AIQQLSTAGN NVDINVEDLT QLVTQFTKDI
910
EQLDSDYFKS VLATSEA
Length:917
Mass (Da):103,313
Last modified:December 6, 2005 - v4
Checksum:i42E1EF87A390ED27
GO

Sequence cautioni

The sequence AAA62406.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti817 – 8171S → P in AAA62406 (PubMed:10077620).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17893 Genomic DNA. Translation: AAA62406.1. Sequence problems.
AE014134 Genomic DNA. Translation: AAF53392.2.
RefSeqiNP_001014482.2. NM_001014482.3.
NP_001246031.1. NM_001259102.2.
NP_001285931.1. NM_001299002.1.
NP_523565.2. NM_078841.5.
UniGeneiDm.2676.

Genome annotation databases

EnsemblMetazoaiFBtr0080630; FBpp0080203; FBgn0015546.
FBtr0309281; FBpp0301208; FBgn0015546.
FBtr0344018; FBpp0310469; FBgn0015546.
FBtr0344019; FBpp0310470; FBgn0015546.
GeneIDi34842.
KEGGidme:Dmel_CG4215.
UCSCiCG4215-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17893 Genomic DNA. Translation: AAA62406.1. Sequence problems.
AE014134 Genomic DNA. Translation: AAF53392.2.
RefSeqiNP_001014482.2. NM_001014482.3.
NP_001246031.1. NM_001259102.2.
NP_001285931.1. NM_001299002.1.
NP_523565.2. NM_078841.5.
UniGeneiDm.2676.

3D structure databases

ProteinModelPortaliP43248.
SMRiP43248. Positions 13-851.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60866. 4 interactions.
DIPiDIP-21499N.
IntActiP43248. 2 interactions.
MINTiMINT-1547583.
STRINGi7227.FBpp0301208.

Proteomic databases

PaxDbiP43248.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0080630; FBpp0080203; FBgn0015546.
FBtr0309281; FBpp0301208; FBgn0015546.
FBtr0344018; FBpp0310469; FBgn0015546.
FBtr0344019; FBpp0310470; FBgn0015546.
GeneIDi34842.
KEGGidme:Dmel_CG4215.
UCSCiCG4215-RA. d. melanogaster.

Organism-specific databases

CTDi34842.
FlyBaseiFBgn0015546. spel1.

Phylogenomic databases

eggNOGiKOG0219. Eukaryota.
COG0249. LUCA.
GeneTreeiENSGT00550000074867.
InParanoidiP43248.
KOiK08735.
OMAiNDWYLAY.
OrthoDBiEOG76DTRW.
PhylomeDBiP43248.

Enzyme and pathway databases

ReactomeiR-DME-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).

Miscellaneous databases

GenomeRNAii34842.
PROiP43248.

Gene expression databases

BgeeiP43248.
ExpressionAtlasiP43248. differential.
GenevisibleiP43248. DM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR011184. DNA_mismatch_repair_MutS.
IPR007695. DNA_mismatch_repair_MutS-lik_N.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR032642. Msh2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11361:SF35. PTHR11361:SF35. 1 hit.
PfamiPF01624. MutS_I. 1 hit.
PF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
PIRSFiPIRSF005813. MSH2. 1 hit.
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Microsatellite instability in Drosophila spellchecker1 (MutS homolog) mutants."
    Flores C., Engels W.
    Proc. Natl. Acad. Sci. U.S.A. 96:2964-2969(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: Canton-S.
    Tissue: Head.
  2. "An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster: the Adh region."
    Ashburner M., Misra S., Roote J., Lewis S.E., Blazej R.G., Davis T., Doyle C., Galle R.F., George R.A., Harris N.L., Hartzell G., Harvey D.A., Hong L., Houston K.A., Hoskins R.A., Johnson G., Martin C., Moshrefi A.R.
    , Palazzolo M., Reese M.G., Spradling A.C., Tsang G., Wan K.H., Whitelaw K., Celniker S.E., Rubin G.M.
    Genetics 153:179-219(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.

Entry informationi

Entry nameiMSH2_DROME
AccessioniPrimary (citable) accession number: P43248
Secondary accession number(s): Q86BK6, Q9NKC4, Q9VJT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 6, 2005
Last modified: June 8, 2016
This is version 137 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.