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Protein

Multidrug resistance protein 1

Gene

Abcb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi426 – 433ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1070 – 1077ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of substances Source: GO_Central
  • ATP binding Source: UniProtKB-KW
  • drug transmembrane transporter activity Source: RGD
  • transporter activity Source: RGD
  • xenobiotic-transporting ATPase activity Source: UniProtKB-EC

GO - Biological processi

  • antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent Source: GO_Central
  • antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent Source: GO_Central
  • antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent Source: GO_Central
  • brain development Source: RGD
  • cellular response to external biotic stimulus Source: RGD
  • cellular response to tumor necrosis factor Source: RGD
  • drug transport Source: RGD
  • establishment of endothelial blood-brain barrier Source: RGD
  • lactation Source: RGD
  • placenta development Source: RGD
  • positive regulation of antigen processing and presentation of peptide antigen via MHC class I Source: GO_Central
  • response to antineoplastic agent Source: RGD
  • response to arsenic-containing substance Source: RGD
  • response to drug Source: RGD
  • response to genistein Source: RGD
  • response to hypoxia Source: RGD
  • response to ionizing radiation Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to mycotoxin Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to steroid hormone Source: RGD
  • response to vitamin A Source: RGD
  • transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein 1 (EC:3.6.3.44)
Alternative name(s):
ATP-binding cassette sub-family B member 1
P-glycoprotein 1
CD_antigen: CD243
Gene namesi
Name:Abcb1
Synonyms:Mdr1, Mdr1b, Pgy1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3318. Abcb1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 42CytoplasmicBy similarityAdd BLAST42
Transmembranei43 – 65HelicalPROSITE-ProRule annotationAdd BLAST23
Topological domaini66 – 115ExtracellularBy similarityAdd BLAST50
Transmembranei116 – 136HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini137 – 185CytoplasmicBy similarityAdd BLAST49
Transmembranei186 – 207HelicalPROSITE-ProRule annotationAdd BLAST22
Topological domaini208 – 214ExtracellularBy similarity7
Transmembranei215 – 235HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini236 – 293CytoplasmicBy similarityAdd BLAST58
Transmembranei294 – 315HelicalPROSITE-ProRule annotationAdd BLAST22
Topological domaini316 – 329ExtracellularBy similarityAdd BLAST14
Transmembranei330 – 351HelicalPROSITE-ProRule annotationAdd BLAST22
Topological domaini352 – 709CytoplasmicBy similarityAdd BLAST358
Transmembranei710 – 730HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini731 – 754ExtracellularBy similarityAdd BLAST24
Transmembranei755 – 775HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini776 – 830CytoplasmicBy similarityAdd BLAST55
Transmembranei831 – 853HelicalPROSITE-ProRule annotationAdd BLAST23
Topological domaini854ExtracellularBy similarity1
Transmembranei855 – 874HelicalPROSITE-ProRule annotationAdd BLAST20
Topological domaini875 – 934CytoplasmicBy similarityAdd BLAST60
Transmembranei935 – 957HelicalPROSITE-ProRule annotationAdd BLAST23
Topological domaini958 – 973ExtracellularBy similarityAdd BLAST16
Transmembranei974 – 995HelicalPROSITE-ProRule annotationAdd BLAST22
Topological domaini996 – 1277CytoplasmicBy similarityAdd BLAST282

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • Golgi membrane Source: RGD
  • integral component of membrane Source: GO_Central
  • membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075229.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933351 – 1277Multidrug resistance protein 1Add BLAST1277

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi91N-linked (GlcNAc...)Sequence analysis1
Glycosylationi96N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP43245.
PRIDEiP43245.

Interactioni

Subunit structurei

Interacts with PSMB5. Finds in a complex with ABCB1, TFPI2 and PPP2R3C; leading to the dephosphorylation of ABCB1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049952.

Structurei

3D structure databases

ProteinModelPortaliP43245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 356ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST308
Domaini391 – 627ABC transporter 1PROSITE-ProRule annotationAdd BLAST237
Domaini709 – 1000ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST292
Domaini1035 – 1272ABC transporter 2PROSITE-ProRule annotationAdd BLAST238

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
HOVERGENiHBG080809.
InParanoidiP43245.
PhylomeDBiP43245.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43245-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFEEGLNGR ADKNFSKMGK KSKKEKEKKP AVGIFGMFRY ADWLDKLCMA
60 70 80 90 100
LGTLAAIIHG TLLPLLMLVF GYMTDSFTPS RDPHSDRAIT NQSEINSTHT
110 120 130 140 150
VSDTSLEEDM AMYAYYYTGI GAGVLIVAYI QVSLWCLAAG RQIHKIRQKF
160 170 180 190 200
FHAIMNQEIG WFDVNDAGEL NTRLTDDVSK INDGIGDKLG MFFQSITTFS
210 220 230 240 250
AGFIIGFISG WKLTLVILAV SPLIGLSSAM WAKVLTSFTN KELQAYAKAG
260 270 280 290 300
AVAEEVLAAI RTVIAFGGQK KELERYNKNL EEAKRVGIKK AITANISIGI
310 320 330 340 350
AYLLVYASYA LAFWYGTSLV LSNEYSIGQV LTVFFSILLG TFSIGHLAPN
360 370 380 390 400
IEAFANARGA AYEIFKIIDN EPSIDSFSTK GHKPDSIMGN LEFKNVYFNY
410 420 430 440 450
PSRSEVKILK GLNLKVKSGQ TVALVGNSGC GKSTTVQLLQ RLYDPIEGEV
460 470 480 490 500
SIDGQDIRTI NVRYLREIIG VVSQEPVLFA TTIAENIRYG RENVTMDEIE
510 520 530 540 550
KAVKEANAYD FIMKLPHKFD TLVGERGAQL SGGQKQRIAI ARALVRNPKI
560 570 580 590 600
LLLDEATSAL DTESEAVVQA ALDKAREGRT TIVIAHRLST VRNADVIAGF
610 620 630 640 650
DGGVIVEQGN HEELMKEKGI YFKLVMTQTR GNEIEPGNNA YESQSDTGAS
660 670 680 690 700
ELTSEESKSP LIRRSIRRSI HRRQDQERRL SSKEDVDEDV PMVSFWQILK
710 720 730 740 750
LNISEWPYLV VGVLCAVING CIQPVFAIVF SKIVGVFSRD DDHETKQRNC
760 770 780 790 800
NLFSLLFLVM GMISFVTYFF QGFTFGKAGE ILTKRLRYMV FKSMLRQDIS
810 820 830 840 850
WFDDHKNTTG SLTTRLASDA SNVKGAMGSR LAVVTQNVAN LGTGIILSLV
860 870 880 890 900
LVYGWQLTLL LVVIIPLIVL GGIIEMKLLS GQALKDKKEL EISGKIATEA
910 920 930 940 950
IENFRTVVSL TREQKFETMY AQSLQIPYRN ALKKAHVFGI TFAFTQAMIY
960 970 980 990 1000
FSYAACFRFG AYLVARELMT FENVMLVFSA VVFGAMAAGN TSSFAPDYAK
1010 1020 1030 1040 1050
AKVSASHIIG IIEKIPEIDS YSTEGLKPNW LEGNVKFNGV KFNYPTRPNI
1060 1070 1080 1090 1100
PVLQGLSFEV KKGQTLRLVG SSGCGKSTVV QLLERFYNPM AGTVFLDGKE
1110 1120 1130 1140 1150
IKQLNVQCVR ALGIVSQEPI LFDCSIAENI AYGDNSRVVS HEEIVRAARE
1160 1170 1180 1190 1200
ANIHQFIDSL PEKYNTRVGD KGTQLSGGQK QRIAIARALV RQPHILLLDE
1210 1220 1230 1240 1250
ATSALDTESE KVVQEALDKA REGRTCVVIA HRLSTIQNAD LIVVIQNGQV
1260 1270
KEHGTHQQLL AQKGIYFSMV QAGAKRS
Length:1,277
Mass (Da):141,387
Last modified:November 1, 1995 - v1
Checksum:i8AFDDD619D2934C1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1227V → I in CAA43415 (PubMed:1348630).Curated1
Sequence conflicti1227V → I in CAA43416 (PubMed:1348630).Curated1
Sequence conflicti1270V → VSV in CAA43415 (PubMed:1348630).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81855 mRNA. No translation available.
X61103 Genomic DNA. Translation: CAA43415.1.
X61104 Genomic DNA. Translation: CAA43416.1.
PIRiJH0502.
UniGeneiRn.144554.
Rn.154810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81855 mRNA. No translation available.
X61103 Genomic DNA. Translation: CAA43415.1.
X61104 Genomic DNA. Translation: CAA43416.1.
PIRiJH0502.
UniGeneiRn.144554.
Rn.154810.

3D structure databases

ProteinModelPortaliP43245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049952.

Chemistry databases

ChEMBLiCHEMBL1075229.

Proteomic databases

PaxDbiP43245.
PRIDEiP43245.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi3318. Abcb1.

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
HOVERGENiHBG080809.
InParanoidiP43245.
PhylomeDBiP43245.

Miscellaneous databases

PROiP43245.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDR1_RAT
AccessioniPrimary (citable) accession number: P43245
Secondary accession number(s): Q63426, Q63427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.