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Protein

Multidrug resistance protein 1

Gene

Abcb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi426 – 433ATP 18
Nucleotide bindingi1070 – 1077ATP 28

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • drug transmembrane transporter activity Source: RGD
  • transporter activity Source: RGD
  • xenobiotic-transporting ATPase activity Source: UniProtKB-EC

GO - Biological processi

  • brain development Source: RGD
  • cellular response to external biotic stimulus Source: RGD
  • cellular response to tumor necrosis factor Source: RGD
  • drug transport Source: RGD
  • establishment of endothelial blood-brain barrier Source: RGD
  • lactation Source: RGD
  • placenta development Source: RGD
  • response to antineoplastic agent Source: RGD
  • response to arsenic-containing substance Source: RGD
  • response to drug Source: RGD
  • response to genistein Source: RGD
  • response to glycoside Source: RGD
  • response to hypoxia Source: RGD
  • response to ionizing radiation Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to mycotoxin Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to steroid hormone Source: RGD
  • response to vitamin A Source: RGD
  • transport Source: RGD

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein 1 (EC:3.6.3.44)
Alternative name(s):
ATP-binding cassette sub-family B member 1
P-glycoprotein 1
CD_antigen: CD243
Gene namesi
Name:Abcb1
Synonyms:Mdr1, Mdr1b, Pgy1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3318. Abcb1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 42CytoplasmicBy similarityAdd BLAST42
Transmembranei43 – 65HelicalAdd BLAST23
Topological domaini66 – 115ExtracellularBy similarityAdd BLAST50
Transmembranei116 – 136HelicalAdd BLAST21
Topological domaini137 – 185CytoplasmicBy similarityAdd BLAST49
Transmembranei186 – 207HelicalAdd BLAST22
Topological domaini208 – 214ExtracellularBy similarity7
Transmembranei215 – 235HelicalAdd BLAST21
Topological domaini236 – 293CytoplasmicBy similarityAdd BLAST58
Transmembranei294 – 315HelicalAdd BLAST22
Topological domaini316 – 329ExtracellularBy similarityAdd BLAST14
Transmembranei330 – 351HelicalAdd BLAST22
Topological domaini352 – 709CytoplasmicBy similarityAdd BLAST358
Transmembranei710 – 730HelicalAdd BLAST21
Topological domaini731 – 754ExtracellularBy similarityAdd BLAST24
Transmembranei755 – 775HelicalAdd BLAST21
Topological domaini776 – 830CytoplasmicBy similarityAdd BLAST55
Transmembranei831 – 853HelicalAdd BLAST23
Topological domaini854ExtracellularBy similarity1
Transmembranei855 – 874HelicalAdd BLAST20
Topological domaini875 – 934CytoplasmicBy similarityAdd BLAST60
Transmembranei935 – 957HelicalAdd BLAST23
Topological domaini958 – 973ExtracellularBy similarityAdd BLAST16
Transmembranei974 – 995HelicalAdd BLAST22
Topological domaini996 – 1277CytoplasmicBy similarityAdd BLAST282

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075229.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933351 – 1277Multidrug resistance protein 1Add BLAST1277

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi91N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi96N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei641PhosphotyrosineBy similarity1
Modified residuei659PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP43245.
PRIDEiP43245.

Interactioni

Subunit structurei

Interacts with PSMB5. Finds in a complex with ABCB1, TFPI2 and PPP2R3C; leading to the dephosphorylation of ABCB1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049952.

Structurei

3D structure databases

ProteinModelPortaliP43245.
SMRiP43245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 356ABC transmembrane type-1 1Add BLAST308
Domaini391 – 627ABC transporter 1Add BLAST237
Domaini709 – 1000ABC transmembrane type-1 2Add BLAST292
Domaini1035 – 1272ABC transporter 2Add BLAST238

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
HOVERGENiHBG080809.
InParanoidiP43245.
PhylomeDBiP43245.

Family and domain databases

Gene3Di1.20.1560.10. 2 hits.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR036640. ABC1_TM_sf.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
SMARTiView protein in SMART
SM00382. AAA. 2 hits.
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiView protein in PROSITE
PS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.

Sequencei

Sequence statusi: Complete.

P43245-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFEEGLNGR ADKNFSKMGK KSKKEKEKKP AVGIFGMFRY ADWLDKLCMA
60 70 80 90 100
LGTLAAIIHG TLLPLLMLVF GYMTDSFTPS RDPHSDRAIT NQSEINSTHT
110 120 130 140 150
VSDTSLEEDM AMYAYYYTGI GAGVLIVAYI QVSLWCLAAG RQIHKIRQKF
160 170 180 190 200
FHAIMNQEIG WFDVNDAGEL NTRLTDDVSK INDGIGDKLG MFFQSITTFS
210 220 230 240 250
AGFIIGFISG WKLTLVILAV SPLIGLSSAM WAKVLTSFTN KELQAYAKAG
260 270 280 290 300
AVAEEVLAAI RTVIAFGGQK KELERYNKNL EEAKRVGIKK AITANISIGI
310 320 330 340 350
AYLLVYASYA LAFWYGTSLV LSNEYSIGQV LTVFFSILLG TFSIGHLAPN
360 370 380 390 400
IEAFANARGA AYEIFKIIDN EPSIDSFSTK GHKPDSIMGN LEFKNVYFNY
410 420 430 440 450
PSRSEVKILK GLNLKVKSGQ TVALVGNSGC GKSTTVQLLQ RLYDPIEGEV
460 470 480 490 500
SIDGQDIRTI NVRYLREIIG VVSQEPVLFA TTIAENIRYG RENVTMDEIE
510 520 530 540 550
KAVKEANAYD FIMKLPHKFD TLVGERGAQL SGGQKQRIAI ARALVRNPKI
560 570 580 590 600
LLLDEATSAL DTESEAVVQA ALDKAREGRT TIVIAHRLST VRNADVIAGF
610 620 630 640 650
DGGVIVEQGN HEELMKEKGI YFKLVMTQTR GNEIEPGNNA YESQSDTGAS
660 670 680 690 700
ELTSEESKSP LIRRSIRRSI HRRQDQERRL SSKEDVDEDV PMVSFWQILK
710 720 730 740 750
LNISEWPYLV VGVLCAVING CIQPVFAIVF SKIVGVFSRD DDHETKQRNC
760 770 780 790 800
NLFSLLFLVM GMISFVTYFF QGFTFGKAGE ILTKRLRYMV FKSMLRQDIS
810 820 830 840 850
WFDDHKNTTG SLTTRLASDA SNVKGAMGSR LAVVTQNVAN LGTGIILSLV
860 870 880 890 900
LVYGWQLTLL LVVIIPLIVL GGIIEMKLLS GQALKDKKEL EISGKIATEA
910 920 930 940 950
IENFRTVVSL TREQKFETMY AQSLQIPYRN ALKKAHVFGI TFAFTQAMIY
960 970 980 990 1000
FSYAACFRFG AYLVARELMT FENVMLVFSA VVFGAMAAGN TSSFAPDYAK
1010 1020 1030 1040 1050
AKVSASHIIG IIEKIPEIDS YSTEGLKPNW LEGNVKFNGV KFNYPTRPNI
1060 1070 1080 1090 1100
PVLQGLSFEV KKGQTLRLVG SSGCGKSTVV QLLERFYNPM AGTVFLDGKE
1110 1120 1130 1140 1150
IKQLNVQCVR ALGIVSQEPI LFDCSIAENI AYGDNSRVVS HEEIVRAARE
1160 1170 1180 1190 1200
ANIHQFIDSL PEKYNTRVGD KGTQLSGGQK QRIAIARALV RQPHILLLDE
1210 1220 1230 1240 1250
ATSALDTESE KVVQEALDKA REGRTCVVIA HRLSTIQNAD LIVVIQNGQV
1260 1270
KEHGTHQQLL AQKGIYFSMV QAGAKRS
Length:1,277
Mass (Da):141,387
Last modified:November 1, 1995 - v1
Checksum:i8AFDDD619D2934C1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1227V → I in CAA43415 (PubMed:1348630).Curated1
Sequence conflicti1227V → I in CAA43416 (PubMed:1348630).Curated1
Sequence conflicti1270V → VSV in CAA43415 (PubMed:1348630).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81855 mRNA. No translation available.
X61103 Genomic DNA. Translation: CAA43415.1.
X61104 Genomic DNA. Translation: CAA43416.1.
PIRiJH0502.
UniGeneiRn.144554.
Rn.154810.

Similar proteinsi

Entry informationi

Entry nameiMDR1_RAT
AccessioniPrimary (citable) accession number: P43245
Secondary accession number(s): Q63426, Q63427
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 22, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families