Multidrug resistance protein 1 - Rattus norvegicus (Rat)

Names and origin

Protein names

Recommended name:
    Multidrug resistance protein 1
    EC=3.6.3.44
Alternative name(s):
    ATP-binding cassette sub-family B member 1
    P-glycoprotein 1
    CD_antigen=CD243

Gene names

Name:	Abcb1
Synonyms:	Mdr1, Mdr1b, Pgy1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	1277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Catalytic activity	ATP + H₂O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Post-translational modification	Several phosphorylated serine residues are present in the linker domain.
Miscellaneous	In mouse the MDR gene family includes three or more related but distinct cellular genes.
Sequence similarities	Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification] Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.

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Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Repeat Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	drug transport Inferred from mutant phenotype. Source: RGD establishment of endothelial blood-brain barrier Inferred from mutant phenotype. Source: RGD response to arsenic Inferred from expression pattern. Source: RGD response to ionizing radiation Inferred from expression pattern. Source: RGD
Cellular component	Golgi membrane Inferred from direct assay. Source: RGD apical plasma membrane Inferred from direct assay. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: RGD xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1277

1277

Multidrug resistance protein 1

PRO_0000093335

Regions

Topological domain

1 – 47

47

Cytoplasmic Potential

Transmembrane

48 – 71

24

Potential

Transmembrane

119 – 139

21

Potential

Transmembrane

199 – 219

21

Potential

Transmembrane

296 – 316

21

Potential

Transmembrane

327 – 347

21

Potential

Topological domain

348 – 709

362

Cytoplasmic Potential

Transmembrane

710 – 730

21

Potential

Transmembrane

755 – 775

21

Potential

Transmembrane

832 – 852

21

Potential

Transmembrane

854 – 874

21

Potential

Transmembrane

937 – 957

21

Potential

Transmembrane

968 – 988

21

Potential

Topological domain

989 – 1277

289

Cytoplasmic Potential

Domain

49 – 356

308

ABC transmembrane type-1 1

Domain

391 – 627

237

ABC transporter 1

Domain

709 – 1000

292

ABC transmembrane type-1 2

Domain

1035 – 1272

238

ABC transporter 2

Nucleotide binding

426 – 433

8

ATP 1 Potential

Nucleotide binding

1070 – 1077

8

ATP 2 Potential

Amino acid modifications

Modified residue

641

1

Phosphotyrosine By similarity

Modified residue

659

1

Phosphoserine By similarity

Glycosylation

91

1

N-linked (GlcNAc...) Potential

Glycosylation

96

1

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

1227

1

V → I in CAA43415. Ref.2

Sequence conflict

1227

1

V → I in CAA43416. Ref.2

Sequence conflict

1270

1

V → VSV in CAA43415. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

P43245-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 8AFDDD619D2934C1
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FASTA

1,277

141,387

        10         20         30         40         50         60 
MEFEEGLNGR ADKNFSKMGK KSKKEKEKKP AVGIFGMFRY ADWLDKLCMA LGTLAAIIHG 

        70         80         90        100        110        120 
TLLPLLMLVF GYMTDSFTPS RDPHSDRAIT NQSEINSTHT VSDTSLEEDM AMYAYYYTGI 

       130        140        150        160        170        180 
GAGVLIVAYI QVSLWCLAAG RQIHKIRQKF FHAIMNQEIG WFDVNDAGEL NTRLTDDVSK 

       190        200        210        220        230        240 
INDGIGDKLG MFFQSITTFS AGFIIGFISG WKLTLVILAV SPLIGLSSAM WAKVLTSFTN 

       250        260        270        280        290        300 
KELQAYAKAG AVAEEVLAAI RTVIAFGGQK KELERYNKNL EEAKRVGIKK AITANISIGI 

       310        320        330        340        350        360 
AYLLVYASYA LAFWYGTSLV LSNEYSIGQV LTVFFSILLG TFSIGHLAPN IEAFANARGA 

       370        380        390        400        410        420 
AYEIFKIIDN EPSIDSFSTK GHKPDSIMGN LEFKNVYFNY PSRSEVKILK GLNLKVKSGQ 

       430        440        450        460        470        480 
TVALVGNSGC GKSTTVQLLQ RLYDPIEGEV SIDGQDIRTI NVRYLREIIG VVSQEPVLFA 

       490        500        510        520        530        540 
TTIAENIRYG RENVTMDEIE KAVKEANAYD FIMKLPHKFD TLVGERGAQL SGGQKQRIAI 

       550        560        570        580        590        600 
ARALVRNPKI LLLDEATSAL DTESEAVVQA ALDKAREGRT TIVIAHRLST VRNADVIAGF 

       610        620        630        640        650        660 
DGGVIVEQGN HEELMKEKGI YFKLVMTQTR GNEIEPGNNA YESQSDTGAS ELTSEESKSP 

       670        680        690        700        710        720 
LIRRSIRRSI HRRQDQERRL SSKEDVDEDV PMVSFWQILK LNISEWPYLV VGVLCAVING 

       730        740        750        760        770        780 
CIQPVFAIVF SKIVGVFSRD DDHETKQRNC NLFSLLFLVM GMISFVTYFF QGFTFGKAGE 

       790        800        810        820        830        840 
ILTKRLRYMV FKSMLRQDIS WFDDHKNTTG SLTTRLASDA SNVKGAMGSR LAVVTQNVAN 

       850        860        870        880        890        900 
LGTGIILSLV LVYGWQLTLL LVVIIPLIVL GGIIEMKLLS GQALKDKKEL EISGKIATEA 

       910        920        930        940        950        960 
IENFRTVVSL TREQKFETMY AQSLQIPYRN ALKKAHVFGI TFAFTQAMIY FSYAACFRFG 

       970        980        990       1000       1010       1020 
AYLVARELMT FENVMLVFSA VVFGAMAAGN TSSFAPDYAK AKVSASHIIG IIEKIPEIDS 

      1030       1040       1050       1060       1070       1080 
YSTEGLKPNW LEGNVKFNGV KFNYPTRPNI PVLQGLSFEV KKGQTLRLVG SSGCGKSTVV 

      1090       1100       1110       1120       1130       1140 
QLLERFYNPM AGTVFLDGKE IKQLNVQCVR ALGIVSQEPI LFDCSIAENI AYGDNSRVVS 

      1150       1160       1170       1180       1190       1200 
HEEIVRAARE ANIHQFIDSL PEKYNTRVGD KGTQLSGGQK QRIAIARALV RQPHILLLDE 

      1210       1220       1230       1240       1250       1260 
ATSALDTESE KVVQEALDKA REGRTCVVIA HRLSTIQNAD LIVVIQNGQV KEHGTHQQLL 

      1270 
AQKGIYFSMV QAGAKRS

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References

[1]	"Cloning and characterization of a member of the rat multidrug resistance (mdr) gene family." Silverman J.A., Raunio H., Gant T.W., Thorgeirsson S.S. Gene 106:229-236(1991) [PubMed: 1682220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Identification of distinct P-glycoprotein gene sequences in rat." Deuchars K.L., Duthie M., Ling V. Biochim. Biophys. Acta 1130:157-165(1992) [PubMed: 1348630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1212-1277. Strain: Sprague-Dawley. Tissue: Liver.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	M81855 mRNA. No translation available. X61103 Genomic DNA. Translation: CAA43415.1. X61104 Genomic DNA. Translation: CAA43416.1.
IPI	IPI00198496.
PIR	JH0502.
UniGene	Rn.144554 Rn.154810
3D structure databases
HSSP	HSSP built from PDB template 1MT0 based on UniProtKB P08716.
ModBase	Search...
Protein-protein interaction databases
STRING	P43245.
Proteomic databases
PRIDE	P43245.
Genome annotation databases
Ensembl	ENSRNOT00000037712; ENSRNOP00000038721; ENSRNOG00000022719; Rattus norvegicus. [Genome view] ENSRNOT00000047126; ENSRNOP00000049952; ENSRNOG00000022719; Rattus norvegicus. [Genome view]
UCSC	NM_012623. rat.
Organism-specific databases
RGD	3318. Abcb1.
Phylogenomic databases
HOVERGEN	P43245.
Enzyme and pathway databases
BRENDA	3.6.3.44. 248.
Gene expression databases
ArrayExpress	P43245.
Genevestigator	P43245.
GermOnline	ENSRNOG00000022719. Rattus norvegicus.
Family and domain databases
InterPro	IPR001140. ABC_TM_transpt. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 2 hits. PF00005. ABC_tran. 2 hits. [Graphical view]
ProDom	PD000006. ABC_transporter. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS50929. ABC_TM1F. 2 hits. PS00211. ABC_TRANSPORTER_1. 2 hits. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

MDR1_RAT

Accession

Primary (citable) accession number: P43245
Secondary accession number(s): Q63426, Q63427

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	October 13, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents