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Protein

Multidrug resistance protein 1

Gene

Abcb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi426 – 4338ATP 1PROSITE-ProRule annotation
Nucleotide bindingi1070 – 10778ATP 2PROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • drug transmembrane transporter activity Source: RGD
  • transporter activity Source: RGD
  • xenobiotic-transporting ATPase activity Source: UniProtKB-EC

GO - Biological processi

  • cellular response to tumor necrosis factor Source: RGD
  • drug transmembrane transport Source: GOC
  • drug transport Source: RGD
  • establishment of endothelial blood-brain barrier Source: RGD
  • lactation Source: RGD
  • placenta development Source: RGD
  • response to arsenic-containing substance Source: RGD
  • response to drug Source: RGD
  • response to hypoxia Source: RGD
  • response to ionizing radiation Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to steroid hormone Source: RGD
  • transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein 1 (EC:3.6.3.44)
Alternative name(s):
ATP-binding cassette sub-family B member 1
P-glycoprotein 1
CD_antigen: CD243
Gene namesi
Name:Abcb1
Synonyms:Mdr1, Mdr1b, Pgy1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3318. Abcb1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4242CytoplasmicBy similarityAdd
BLAST
Transmembranei43 – 6523HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini66 – 11550ExtracellularBy similarityAdd
BLAST
Transmembranei116 – 13621HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini137 – 18549CytoplasmicBy similarityAdd
BLAST
Transmembranei186 – 20722HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini208 – 2147ExtracellularBy similarity
Transmembranei215 – 23521HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini236 – 29358CytoplasmicBy similarityAdd
BLAST
Transmembranei294 – 31522HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini316 – 32914ExtracellularBy similarityAdd
BLAST
Transmembranei330 – 35122HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini352 – 709358CytoplasmicBy similarityAdd
BLAST
Transmembranei710 – 73021HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini731 – 75424ExtracellularBy similarityAdd
BLAST
Transmembranei755 – 77521HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini776 – 83055CytoplasmicBy similarityAdd
BLAST
Transmembranei831 – 85323HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini854 – 8541ExtracellularBy similarity
Transmembranei855 – 87420HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini875 – 93460CytoplasmicBy similarityAdd
BLAST
Transmembranei935 – 95723HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini958 – 97316ExtracellularBy similarityAdd
BLAST
Transmembranei974 – 99522HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini996 – 1277282CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • Golgi membrane Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1075229.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12771277Multidrug resistance protein 1PRO_0000093335Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence analysis
Glycosylationi96 – 961N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP43245.
PRIDEiP43245.

PTM databases

PhosphoSiteiP43245.

Interactioni

Subunit structurei

Interacts with PSMB5. Finds in a complex with ABCB1, TFPI2 and PPP2R3C; leading to the dephosphorylation of ABCB1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049952.

Structurei

3D structure databases

ProteinModelPortaliP43245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini49 – 356308ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini391 – 627237ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini709 – 1000292ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini1035 – 1272238ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
HOVERGENiHBG080809.
InParanoidiP43245.
PhylomeDBiP43245.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43245-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFEEGLNGR ADKNFSKMGK KSKKEKEKKP AVGIFGMFRY ADWLDKLCMA
60 70 80 90 100
LGTLAAIIHG TLLPLLMLVF GYMTDSFTPS RDPHSDRAIT NQSEINSTHT
110 120 130 140 150
VSDTSLEEDM AMYAYYYTGI GAGVLIVAYI QVSLWCLAAG RQIHKIRQKF
160 170 180 190 200
FHAIMNQEIG WFDVNDAGEL NTRLTDDVSK INDGIGDKLG MFFQSITTFS
210 220 230 240 250
AGFIIGFISG WKLTLVILAV SPLIGLSSAM WAKVLTSFTN KELQAYAKAG
260 270 280 290 300
AVAEEVLAAI RTVIAFGGQK KELERYNKNL EEAKRVGIKK AITANISIGI
310 320 330 340 350
AYLLVYASYA LAFWYGTSLV LSNEYSIGQV LTVFFSILLG TFSIGHLAPN
360 370 380 390 400
IEAFANARGA AYEIFKIIDN EPSIDSFSTK GHKPDSIMGN LEFKNVYFNY
410 420 430 440 450
PSRSEVKILK GLNLKVKSGQ TVALVGNSGC GKSTTVQLLQ RLYDPIEGEV
460 470 480 490 500
SIDGQDIRTI NVRYLREIIG VVSQEPVLFA TTIAENIRYG RENVTMDEIE
510 520 530 540 550
KAVKEANAYD FIMKLPHKFD TLVGERGAQL SGGQKQRIAI ARALVRNPKI
560 570 580 590 600
LLLDEATSAL DTESEAVVQA ALDKAREGRT TIVIAHRLST VRNADVIAGF
610 620 630 640 650
DGGVIVEQGN HEELMKEKGI YFKLVMTQTR GNEIEPGNNA YESQSDTGAS
660 670 680 690 700
ELTSEESKSP LIRRSIRRSI HRRQDQERRL SSKEDVDEDV PMVSFWQILK
710 720 730 740 750
LNISEWPYLV VGVLCAVING CIQPVFAIVF SKIVGVFSRD DDHETKQRNC
760 770 780 790 800
NLFSLLFLVM GMISFVTYFF QGFTFGKAGE ILTKRLRYMV FKSMLRQDIS
810 820 830 840 850
WFDDHKNTTG SLTTRLASDA SNVKGAMGSR LAVVTQNVAN LGTGIILSLV
860 870 880 890 900
LVYGWQLTLL LVVIIPLIVL GGIIEMKLLS GQALKDKKEL EISGKIATEA
910 920 930 940 950
IENFRTVVSL TREQKFETMY AQSLQIPYRN ALKKAHVFGI TFAFTQAMIY
960 970 980 990 1000
FSYAACFRFG AYLVARELMT FENVMLVFSA VVFGAMAAGN TSSFAPDYAK
1010 1020 1030 1040 1050
AKVSASHIIG IIEKIPEIDS YSTEGLKPNW LEGNVKFNGV KFNYPTRPNI
1060 1070 1080 1090 1100
PVLQGLSFEV KKGQTLRLVG SSGCGKSTVV QLLERFYNPM AGTVFLDGKE
1110 1120 1130 1140 1150
IKQLNVQCVR ALGIVSQEPI LFDCSIAENI AYGDNSRVVS HEEIVRAARE
1160 1170 1180 1190 1200
ANIHQFIDSL PEKYNTRVGD KGTQLSGGQK QRIAIARALV RQPHILLLDE
1210 1220 1230 1240 1250
ATSALDTESE KVVQEALDKA REGRTCVVIA HRLSTIQNAD LIVVIQNGQV
1260 1270
KEHGTHQQLL AQKGIYFSMV QAGAKRS
Length:1,277
Mass (Da):141,387
Last modified:November 1, 1995 - v1
Checksum:i8AFDDD619D2934C1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1227 – 12271V → I in CAA43415 (PubMed:1348630).Curated
Sequence conflicti1227 – 12271V → I in CAA43416 (PubMed:1348630).Curated
Sequence conflicti1270 – 12701V → VSV in CAA43415 (PubMed:1348630).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81855 mRNA. No translation available.
X61103 Genomic DNA. Translation: CAA43415.1.
X61104 Genomic DNA. Translation: CAA43416.1.
PIRiJH0502.
UniGeneiRn.144554.
Rn.154810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81855 mRNA. No translation available.
X61103 Genomic DNA. Translation: CAA43415.1.
X61104 Genomic DNA. Translation: CAA43416.1.
PIRiJH0502.
UniGeneiRn.144554.
Rn.154810.

3D structure databases

ProteinModelPortaliP43245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049952.

Chemistry

ChEMBLiCHEMBL1075229.

PTM databases

PhosphoSiteiP43245.

Proteomic databases

PaxDbiP43245.
PRIDEiP43245.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi3318. Abcb1.

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
HOVERGENiHBG080809.
InParanoidiP43245.
PhylomeDBiP43245.

Miscellaneous databases

PROiP43245.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of a member of the rat multidrug resistance (mdr) gene family."
    Silverman J.A., Raunio H., Gant T.W., Thorgeirsson S.S.
    Gene 106:229-236(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification of distinct P-glycoprotein gene sequences in rat."
    Deuchars K.L., Duthie M., Ling V.
    Biochim. Biophys. Acta 1130:157-165(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1212-1277.
    Strain: Sprague-Dawley.
    Tissue: Liver.

Entry informationi

Entry nameiMDR1_RAT
AccessioniPrimary (citable) accession number: P43245
Secondary accession number(s): Q63426, Q63427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 11, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.