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P43241 (CDX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein CDX-2
Alternative name(s):
Caudal-type homeobox protein 2
Gene names
Name:Cdx2
Synonyms:Cdx-2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.

Subcellular location

Nucleus.

Tissue specificity

Intestine; expressed specifically in gut epithelium where it is not restricted to a particular cell lineage. Abundant expression is seen in the proximal colon with slightly lower levels in distal colon. Expression in the proximal colon is not restricted either to a particular cell lineage or stage of differentiation while in the distal colon it is more abundant in the differentiated cells towards the top of the crypt.

Post-translational modification

Phosphorylation of Ser-60 mediates the transactivation capacity.

Sequence similarities

Belongs to the Caudal homeobox family.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanterior/posterior pattern specification

Inferred from genetic interaction PubMed 19853565. Source: MGI

blastocyst development

Inferred from mutant phenotype PubMed 15788452. Source: MGI

blood vessel development

Inferred from genetic interaction PubMed 16396910. Source: MGI

cell differentiation

Inferred from mutant phenotype PubMed 15788452. Source: MGI

endosome to lysosome transport

Inferred from mutant phenotype PubMed 20551175. Source: MGI

establishment or maintenance of epithelial cell apical/basal polarity

Inferred from mutant phenotype PubMed 20551175. Source: MGI

labyrinthine layer development

Inferred from genetic interaction PubMed 19853565. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16325584. Source: MGI

pattern specification process

Inferred from mutant phenotype PubMed 11959827. Source: MGI

placenta development

Inferred from genetic interaction PubMed 16396910. Source: MGI

positive regulation of cell differentiation

Inferred from direct assay PubMed 16325584. Source: MGI

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 19796622. Source: MGI

somatic stem cell maintenance

Inferred from mutant phenotype PubMed 16325584PubMed 20404091. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

trophectodermal cell differentiation

Inferred from direct assay PubMed 16325584. Source: MGI

   Cellular_componentcondensed nuclear chromosome

Inferred from direct assay PubMed 15509711. Source: MGI

nucleus

Inferred from direct assay PubMed 16325584PubMed 17138661PubMed 18423437PubMed 19796622PubMed 20676102PubMed 21530438PubMed 22573614PubMed 22948967. Source: MGI

protein complex

Inferred from direct assay PubMed 19796622. Source: MGI

transcriptional repressor complex

Inferred from direct assay PubMed 16325584. Source: MGI

   Molecular_functiondouble-stranded DNA binding

Inferred from electronic annotation. Source: Ensembl

sequence-specific DNA binding

Inferred from direct assay PubMed 19796622. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

transcription corepressor activity

Inferred from direct assay PubMed 16325584. Source: MGI

transcription regulatory region sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311Homeobox protein CDX-2
PRO_0000048851

Regions

DNA binding185 – 24460Homeobox
Compositional bias46 – 538Poly-Ala
Compositional bias85 – 928Poly-Ala
Compositional bias247 – 25711Poly-Gln

Amino acid modifications

Modified residue601Phosphoserine Ref.4

Experimental info

Sequence conflict691Y → H in AAB32251. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P43241 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 71FFC4C263462FF3

FASTA31133,476
        10         20         30         40         50         60 
MYVSYLLDKD VSMYPSSVRH SGGLNLAPQN FVSPPQYPDY GGYHVAAAAA ATANLDSAQS 

        70         80         90        100        110        120 
PGPSWPTAYG APLREDWNGY APGGAAAANA VAHGLNGGSP AAAMGYSSPA EYHAHHHPHH 

       130        140        150        160        170        180 
HPHHPAASPS CASGLLQTLN LGPPGPAATA AAEQLSPSGQ RRNLCEWMRK PAQQSLGSQV 

       190        200        210        220        230        240 
KTRTKDKYRV VYTDHQRLEL EKEFHFSRYI TIRRKSELAA TLGLSERQVK IWFQNRRAKE 

       250        260        270        280        290        300 
RKIKKKQQQQ QQQQQQQPPQ PPPQPSQPQP GALRSVPEPL SPVTSLQGSV PGSVPGVLGP 

       310 
AGGVLNSTVT Q 

« Hide

References

[1]"Structure of the murine homeobox gene cdx-2. Expression in embryonic and adult intestinal epithelium."
James R.J., Erler T., Kazenwadel J.
J. Biol. Chem. 269:15229-15237(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: BALB/c.
[2]"A homeodomain protein related to caudal regulates intestine-specific gene transcription."
Suh E., Chen L., Taylor J., Traber P.G.
Mol. Cell. Biol. 14:7340-7351(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Small intestine.
[3]"Homeobox gene expression in the intestinal epithelium of adult mice."
James R.J., Kazenwadel J.
J. Biol. Chem. 266:3246-3251(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 204-229.
[4]"Phosphorylation of the serine 60 residue within the Cdx2 activation domain mediates its transactivation capacity."
Rings E.H., Boudreau F., Taylor J.K., Moffett J., Suh E.R., Traber P.G.
Gastroenterology 121:1437-1450(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-60.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00454 Unassigned DNA. Translation: AAA19645.1.
S74520 mRNA. Translation: AAB32251.1.
PIRA53808.
RefSeqNP_031699.2. NM_007673.3.
UniGeneMm.20358.

3D structure databases

ProteinModelPortalP43241.
SMRP43241. Positions 189-242.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198664. 6 interactions.
STRING10090.ENSMUSP00000031650.

PTM databases

PhosphoSiteP43241.

Proteomic databases

PRIDEP43241.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000031650; ENSMUSP00000031650; ENSMUSG00000029646.
GeneID12591.
KEGGmmu:12591.
UCSCuc009anz.1. mouse.

Organism-specific databases

CTD1045.
MGIMGI:88361. Cdx2.

Phylogenomic databases

eggNOGNOG304047.
HOGENOMHOG000115975.
HOVERGENHBG005302.
InParanoidP43241.
KOK09312.
OMAGPSWPSP.
OrthoDBEOG71P2BK.
PhylomeDBP43241.
TreeFamTF351605.

Gene expression databases

BgeeP43241.
CleanExMM_CDX2.
GenevestigatorP43241.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR006820. Caudal_activation_dom.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view]
PfamPF04731. Caudal_act. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio281738.
PROP43241.
SOURCESearch...

Entry information

Entry nameCDX2_MOUSE
AccessionPrimary (citable) accession number: P43241
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot