P43241 (CDX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeobox protein CDX-2

Alternative name(s):
Caudal-type homeobox protein 2

Gene names

Name:	Cdx2
Synonyms:	Cdx-2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
Subcellular location	Nucleus.
Tissue specificity	Intestine; expressed specifically in gut epithelium where it is not restricted to a particular cell lineage. Abundant expression is seen in the proximal colon with slightly lower levels in distal colon. Expression in the proximal colon is not restricted either to a particular cell lineage or stage of differentiation while in the distal colon it is more abundant in the differentiated cells towards the top of the crypt.
Post-translational modification	Phosphorylation of Ser-60 mediates the transactivation capacity.
Sequence similarities	Belongs to the Caudal homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Activator Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anterior/posterior pattern specification Inferred from genetic interaction PubMed 19853565. Source: MGI blood vessel development Inferred from genetic interaction PubMed 16396910. Source: MGI endosome to lysosome transport Inferred from mutant phenotype PubMed 20551175. Source: MGI establishment or maintenance of epithelial cell apical/basal polarity Inferred from mutant phenotype PubMed 20551175. Source: MGI labyrinthine layer development Inferred from genetic interaction PubMed 19853565. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 16325584. Source: MGI positive regulation of cell differentiation Inferred from direct assay PubMed 16325584. Source: MGI somatic stem cell maintenance Inferred from mutant phenotype PubMed 16325584PubMed 20404091. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW trophectodermal cell differentiation Inferred from direct assay PubMed 16325584. Source: MGI
Cellular_component	condensed nuclear chromosome Inferred from direct assay PubMed 15509711. Source: MGI transcriptional repressor complex Inferred from direct assay PubMed 16325584. Source: MGI
Molecular_function	sequence-specific DNA binding Inferred from direct assay PubMed 19796622. Source: MGI sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro transcription corepressor activity Inferred from direct assay PubMed 16325584. Source: MGI transcription regulatory region sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Homeobox protein CDX-2		PRO_0000048851
Regions
	DNA binding	185 – 244	60	Homeobox
	Compositional bias	46 – 53	8	Poly-Ala
	Compositional bias	85 – 92	8	Poly-Ala
	Compositional bias	247 – 257	11	Poly-Gln
Amino acid modifications
	Modified residue	60	1	Phosphoserine Ref.4
Experimental info
	Sequence conflict	69	1	Y → H in AAB32251. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P43241 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 71FFC4C263462FF3	FASTA	311	33,476
	10 20 30 40 50 60 MYVSYLLDKD VSMYPSSVRH SGGLNLAPQN FVSPPQYPDY GGYHVAAAAA ATANLDSAQS 70 80 90 100 110 120 PGPSWPTAYG APLREDWNGY APGGAAAANA VAHGLNGGSP AAAMGYSSPA EYHAHHHPHH 130 140 150 160 170 180 HPHHPAASPS CASGLLQTLN LGPPGPAATA AAEQLSPSGQ RRNLCEWMRK PAQQSLGSQV 190 200 210 220 230 240 KTRTKDKYRV VYTDHQRLEL EKEFHFSRYI TIRRKSELAA TLGLSERQVK IWFQNRRAKE 250 260 270 280 290 300 RKIKKKQQQQ QQQQQQQPPQ PPPQPSQPQP GALRSVPEPL SPVTSLQGSV PGSVPGVLGP 310 AGGVLNSTVT Q

References

[1]	"Structure of the murine homeobox gene cdx-2. Expression in embryonic and adult intestinal epithelium." James R.J., Erler T., Kazenwadel J. J. Biol. Chem. 269:15229-15237(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: BALB/c.
[2]	"A homeodomain protein related to caudal regulates intestine-specific gene transcription." Suh E., Chen L., Taylor J., Traber P.G. Mol. Cell. Biol. 14:7340-7351(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Small intestine.
[3]	"Homeobox gene expression in the intestinal epithelium of adult mice." James R.J., Kazenwadel J. J. Biol. Chem. 266:3246-3251(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 204-229.
[4]	"Phosphorylation of the serine 60 residue within the Cdx2 activation domain mediates its transactivation capacity." Rings E.H., Boudreau F., Taylor J.K., Moffett J., Suh E.R., Traber P.G. Gastroenterology 121:1437-1450(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-60.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00454 Unassigned DNA. Translation: AAA19645.1. S74520 mRNA. Translation: AAB32251.1.
IPI	IPI00320937.
PIR	A53808.
RefSeq	NP_031699.2. NM_007673.3.
UniGene	Mm.20358.
3D structure databases
ProteinModelPortal	P43241.
SMR	P43241. Positions 189-242.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000031650.
PTM databases
PhosphoSite	P43241.
Proteomic databases
PRIDE	P43241.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000031650; ENSMUSP00000031650; ENSMUSG00000029646.
GeneID	12591.
KEGG	mmu:12591.
UCSC	uc009anz.1. mouse.
Organism-specific databases
CTD	1045.
MGI	MGI:88361. Cdx2.
Phylogenomic databases
eggNOG	NOG304047.
HOGENOM	HOG000115975.
HOVERGEN	HBG005302.
InParanoid	P43241.
KO	K09312.
OMA	WMRKPTQ.
OrthoDB	EOG42FSK7.
Gene expression databases
ArrayExpress	P43241.
Bgee	P43241.
CleanEx	MM_CDX2.
Genevestigator	P43241.
GermOnline	ENSMUSG00000029646. Mus musculus.
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR006820. Caudal_activation_dom. IPR017970. Homeobox_CS. IPR020479. Homeobox_metazoa. IPR001356. Homeodomain. IPR009057. Homeodomain-like. IPR000047. HTH_motif. [Graphical view]
Pfam	PF04731. Caudal_act. 1 hit. PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00024. HOMEOBOX. PR00031. HTHREPRESSR.
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	281738.
SOURCE	Search...

Entry information

Entry name

CDX2_MOUSE

Accession

Primary (citable) accession number: P43241

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 29, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program