P43241 (CDX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeobox protein CDX-2

Alternative name(s):
Caudal-type homeobox protein 2

Gene names

Name:	Cdx2
Synonyms:	Cdx-2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
Subcellular location	Nucleus.
Tissue specificity	Intestine; expressed specifically in gut epithelium where it is not restricted to a particular cell lineage. Abundant expression is seen in the proximal colon with slightly lower levels in distal colon. Expression in the proximal colon is not restricted either to a particular cell lineage or stage of differentiation while in the distal colon it is more abundant in the differentiated cells towards the top of the crypt.
Post-translational modification	Phosphorylation of Ser-60 mediates the transactivation capacity.
Sequence similarities	Belongs to the Caudal homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Activator Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anterior/posterior pattern specification Inferred from genetic interaction. Source: MGI blood vessel development Inferred from genetic interaction. Source: MGI labyrinthine layer development Inferred from genetic interaction. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI positive regulation of cell differentiation Inferred from direct assay. Source: MGI somatic stem cell maintenance Inferred from mutant phenotype. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW trophectodermal cell differentiation Inferred from direct assay. Source: MGI
Cellular component	condensed nuclear chromosome Inferred from direct assay. Source: MGI transcriptional repressor complex Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: MGI sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro transcription corepressor activity Inferred from direct assay. Source: MGI transcription regulatory region sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Homeobox protein CDX-2		PRO_0000048851
Regions
	DNA binding	185 – 244	60	Homeobox
	Compositional bias	46 – 53	8	Poly-Ala
	Compositional bias	85 – 92	8	Poly-Ala
	Compositional bias	247 – 257	11	Poly-Gln
Amino acid modifications
	Modified residue	60	1	Phosphoserine Ref.4
Experimental info
	Sequence conflict	69	1	Y → H in AAB32251. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P43241 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 71FFC4C263462FF3	FASTA	311	33,476
	10 20 30 40 50 60 MYVSYLLDKD VSMYPSSVRH SGGLNLAPQN FVSPPQYPDY GGYHVAAAAA ATANLDSAQS 70 80 90 100 110 120 PGPSWPTAYG APLREDWNGY APGGAAAANA VAHGLNGGSP AAAMGYSSPA EYHAHHHPHH 130 140 150 160 170 180 HPHHPAASPS CASGLLQTLN LGPPGPAATA AAEQLSPSGQ RRNLCEWMRK PAQQSLGSQV 190 200 210 220 230 240 KTRTKDKYRV VYTDHQRLEL EKEFHFSRYI TIRRKSELAA TLGLSERQVK IWFQNRRAKE 250 260 270 280 290 300 RKIKKKQQQQ QQQQQQQPPQ PPPQPSQPQP GALRSVPEPL SPVTSLQGSV PGSVPGVLGP 310 AGGVLNSTVT Q

References

[1]	"Structure of the murine homeobox gene cdx-2. Expression in embryonic and adult intestinal epithelium." James R.J., Erler T., Kazenwadel J. J. Biol. Chem. 269:15229-15237(1994) [PubMed: 7910823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: BALB/c.
[2]	"A homeodomain protein related to caudal regulates intestine-specific gene transcription." Suh E., Chen L., Taylor J., Traber P.G. Mol. Cell. Biol. 14:7340-7351(1994) [PubMed: 7935448] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Small intestine.
[3]	"Homeobox gene expression in the intestinal epithelium of adult mice." James R.J., Kazenwadel J. J. Biol. Chem. 266:3246-3251(1991) [PubMed: 1671571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 204-229.
[4]	"Phosphorylation of the serine 60 residue within the Cdx2 activation domain mediates its transactivation capacity." Rings E.H., Boudreau F., Taylor J.K., Moffett J., Suh E.R., Traber P.G. Gastroenterology 121:1437-1450(2001) [PubMed: 11729123] [Abstract] Cited for: PHOSPHORYLATION AT SER-60.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00454 Unassigned DNA. Translation: AAA19645.1. S74520 mRNA. Translation: AAB32251.1.
IPI	IPI00320937.
PIR	A53808.
RefSeq	NP_031699.2. NM_007673.3.
UniGene	Mm.20358.
3D structure databases
ProteinModelPortal	P43241.
SMR	P43241. Positions 189-242.
ModBase	Search...
Protein-protein interaction databases
STRING	P43241.
PTM databases
PhosphoSite	P43241.
Proteomic databases
PRIDE	P43241.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000031650; ENSMUSP00000031650; ENSMUSG00000029646.
GeneID	12591.
KEGG	mmu:12591.
Organism-specific databases
CTD	1045.
MGI	MGI:88361. Cdx2.
Phylogenomic databases
eggNOG	roNOG09317.
HOGENOM	HBG444180.
HOVERGEN	HBG005302.
InParanoid	P43241.
OMA	WMRKPTQ.
OrthoDB	EOG42FSK7.
PhylomeDB	P43241.
Gene expression databases
ArrayExpress	P43241.
Bgee	P43241.
CleanEx	MM_CDX2.
Genevestigator	P43241.
GermOnline	ENSMUSG00000029646. Mus musculus.
Family and domain databases
InterPro	IPR006820. Caudal_activation_dom. IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR020479. Homeobox_eu. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. IPR000047. HTH_motif. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
KO	K09312.
Pfam	PF04731. Caudal_act. 1 hit. PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00024. HOMEOBOX. PR00031. HTHREPRESSR.
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	281738.
SOURCE	Search...

Entry information

Entry name

CDX2_MOUSE

Accession

Primary (citable) accession number: P43241

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program