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P43241

- CDX2_MOUSE

UniProt

P43241 - CDX2_MOUSE

Protein

Homeobox protein CDX-2

Gene

Cdx2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi185 – 24460HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. double-stranded DNA binding Source: Ensembl
    2. protein binding Source: MGI
    3. sequence-specific DNA binding Source: MGI
    4. sequence-specific DNA binding transcription factor activity Source: Ensembl
    5. transcription corepressor activity Source: MGI
    6. transcription regulatory region sequence-specific DNA binding Source: InterPro

    GO - Biological processi

    1. anterior/posterior pattern specification Source: MGI
    2. blastocyst development Source: MGI
    3. blood vessel development Source: MGI
    4. cell differentiation Source: MGI
    5. endosome to lysosome transport Source: MGI
    6. establishment or maintenance of epithelial cell apical/basal polarity Source: MGI
    7. labyrinthine layer development Source: MGI
    8. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    9. pattern specification process Source: MGI
    10. placenta development Source: MGI
    11. positive regulation of cell differentiation Source: MGI
    12. positive regulation of cell proliferation Source: Ensembl
    13. positive regulation of transcription, DNA-templated Source: Ensembl
    14. regulation of transcription from RNA polymerase II promoter Source: MGI
    15. somatic stem cell maintenance Source: MGI
    16. transcription, DNA-templated Source: UniProtKB-KW
    17. trophectodermal cell differentiation Source: MGI

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homeobox protein CDX-2
    Alternative name(s):
    Caudal-type homeobox protein 2
    Gene namesi
    Name:Cdx2
    Synonyms:Cdx-2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:88361. Cdx2.

    Subcellular locationi

    GO - Cellular componenti

    1. condensed nuclear chromosome Source: MGI
    2. nucleus Source: MGI
    3. protein complex Source: MGI
    4. transcriptional repressor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 311311Homeobox protein CDX-2PRO_0000048851Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei60 – 601Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylation of Ser-60 mediates the transactivation capacity.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP43241.
    PRIDEiP43241.

    PTM databases

    PhosphoSiteiP43241.

    Expressioni

    Tissue specificityi

    Intestine; expressed specifically in gut epithelium where it is not restricted to a particular cell lineage. Abundant expression is seen in the proximal colon with slightly lower levels in distal colon. Expression in the proximal colon is not restricted either to a particular cell lineage or stage of differentiation while in the distal colon it is more abundant in the differentiated cells towards the top of the crypt.

    Gene expression databases

    BgeeiP43241.
    CleanExiMM_CDX2.
    GenevestigatoriP43241.

    Interactioni

    Protein-protein interaction databases

    BioGridi198664. 6 interactions.
    STRINGi10090.ENSMUSP00000031650.

    Structurei

    3D structure databases

    ProteinModelPortaliP43241.
    SMRiP43241. Positions 189-242.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi46 – 538Poly-Ala
    Compositional biasi85 – 928Poly-Ala
    Compositional biasi247 – 25711Poly-GlnAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Caudal homeobox family.Curated
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG304047.
    HOGENOMiHOG000115975.
    HOVERGENiHBG005302.
    InParanoidiP43241.
    KOiK09312.
    OMAiGPSWPSP.
    OrthoDBiEOG71P2BK.
    PhylomeDBiP43241.
    TreeFamiTF351605.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR006820. Caudal_activation_dom.
    IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR020479. Homeobox_metazoa.
    IPR009057. Homeodomain-like.
    IPR000047. HTH_motif.
    [Graphical view]
    PfamiPF04731. Caudal_act. 1 hit.
    PF00046. Homeobox. 1 hit.
    [Graphical view]
    PRINTSiPR00024. HOMEOBOX.
    PR00031. HTHREPRESSR.
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43241-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYVSYLLDKD VSMYPSSVRH SGGLNLAPQN FVSPPQYPDY GGYHVAAAAA    50
    ATANLDSAQS PGPSWPTAYG APLREDWNGY APGGAAAANA VAHGLNGGSP 100
    AAAMGYSSPA EYHAHHHPHH HPHHPAASPS CASGLLQTLN LGPPGPAATA 150
    AAEQLSPSGQ RRNLCEWMRK PAQQSLGSQV KTRTKDKYRV VYTDHQRLEL 200
    EKEFHFSRYI TIRRKSELAA TLGLSERQVK IWFQNRRAKE RKIKKKQQQQ 250
    QQQQQQQPPQ PPPQPSQPQP GALRSVPEPL SPVTSLQGSV PGSVPGVLGP 300
    AGGVLNSTVT Q 311
    Length:311
    Mass (Da):33,476
    Last modified:November 1, 1995 - v1
    Checksum:i71FFC4C263462FF3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti69 – 691Y → H in AAB32251. (PubMed:7935448)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00454 Unassigned DNA. Translation: AAA19645.1.
    S74520 mRNA. Translation: AAB32251.1.
    CCDSiCCDS19878.1.
    PIRiA53808.
    RefSeqiNP_031699.2. NM_007673.3.
    UniGeneiMm.20358.

    Genome annotation databases

    EnsembliENSMUST00000031650; ENSMUSP00000031650; ENSMUSG00000029646.
    GeneIDi12591.
    KEGGimmu:12591.
    UCSCiuc009anz.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00454 Unassigned DNA. Translation: AAA19645.1 .
    S74520 mRNA. Translation: AAB32251.1 .
    CCDSi CCDS19878.1.
    PIRi A53808.
    RefSeqi NP_031699.2. NM_007673.3.
    UniGenei Mm.20358.

    3D structure databases

    ProteinModelPortali P43241.
    SMRi P43241. Positions 189-242.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198664. 6 interactions.
    STRINGi 10090.ENSMUSP00000031650.

    PTM databases

    PhosphoSitei P43241.

    Proteomic databases

    MaxQBi P43241.
    PRIDEi P43241.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031650 ; ENSMUSP00000031650 ; ENSMUSG00000029646 .
    GeneIDi 12591.
    KEGGi mmu:12591.
    UCSCi uc009anz.1. mouse.

    Organism-specific databases

    CTDi 1045.
    MGIi MGI:88361. Cdx2.

    Phylogenomic databases

    eggNOGi NOG304047.
    HOGENOMi HOG000115975.
    HOVERGENi HBG005302.
    InParanoidi P43241.
    KOi K09312.
    OMAi GPSWPSP.
    OrthoDBi EOG71P2BK.
    PhylomeDBi P43241.
    TreeFami TF351605.

    Miscellaneous databases

    NextBioi 281738.
    PROi P43241.
    SOURCEi Search...

    Gene expression databases

    Bgeei P43241.
    CleanExi MM_CDX2.
    Genevestigatori P43241.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR006820. Caudal_activation_dom.
    IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR020479. Homeobox_metazoa.
    IPR009057. Homeodomain-like.
    IPR000047. HTH_motif.
    [Graphical view ]
    Pfami PF04731. Caudal_act. 1 hit.
    PF00046. Homeobox. 1 hit.
    [Graphical view ]
    PRINTSi PR00024. HOMEOBOX.
    PR00031. HTHREPRESSR.
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the murine homeobox gene cdx-2. Expression in embryonic and adult intestinal epithelium."
      James R.J., Erler T., Kazenwadel J.
      J. Biol. Chem. 269:15229-15237(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: BALB/c.
    2. "A homeodomain protein related to caudal regulates intestine-specific gene transcription."
      Suh E., Chen L., Taylor J., Traber P.G.
      Mol. Cell. Biol. 14:7340-7351(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Small intestine.
    3. "Homeobox gene expression in the intestinal epithelium of adult mice."
      James R.J., Kazenwadel J.
      J. Biol. Chem. 266:3246-3251(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 204-229.
    4. "Phosphorylation of the serine 60 residue within the Cdx2 activation domain mediates its transactivation capacity."
      Rings E.H., Boudreau F., Taylor J.K., Moffett J., Suh E.R., Traber P.G.
      Gastroenterology 121:1437-1450(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-60.

    Entry informationi

    Entry nameiCDX2_MOUSE
    AccessioniPrimary (citable) accession number: P43241
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3