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Protein

Homeobox protein CDX-2

Gene

Cdx2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi185 – 24460HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. double-stranded DNA binding Source: Ensembl
  2. RNA polymerase II distal enhancer sequence-specific DNA binding Source: MGI
  3. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: MGI
  4. sequence-specific DNA binding Source: MGI
  5. transcription corepressor activity Source: MGI

GO - Biological processi

  1. anterior/posterior pattern specification Source: MGI
  2. blastocyst development Source: MGI
  3. blood vessel development Source: MGI
  4. cell differentiation Source: MGI
  5. endosome to lysosome transport Source: MGI
  6. establishment or maintenance of epithelial cell apical/basal polarity Source: MGI
  7. labyrinthine layer development Source: MGI
  8. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  9. pattern specification process Source: MGI
  10. placenta development Source: MGI
  11. positive regulation of cell differentiation Source: MGI
  12. positive regulation of cell proliferation Source: Ensembl
  13. positive regulation of transcription, DNA-templated Source: Ensembl
  14. regulation of transcription from RNA polymerase II promoter Source: MGI
  15. somatic stem cell maintenance Source: MGI
  16. trophectodermal cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_324802. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein CDX-2
Alternative name(s):
Caudal-type homeobox protein 2
Gene namesi
Name:Cdx2
Synonyms:Cdx-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:88361. Cdx2.

Subcellular locationi

GO - Cellular componenti

  1. condensed nuclear chromosome Source: MGI
  2. nucleoplasm Source: MGI
  3. nucleus Source: MGI
  4. protein complex Source: MGI
  5. transcriptional repressor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311Homeobox protein CDX-2PRO_0000048851Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei60 – 601Phosphoserine1 Publication

Post-translational modificationi

Phosphorylation of Ser-60 mediates the transactivation capacity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43241.
PRIDEiP43241.

PTM databases

PhosphoSiteiP43241.

Expressioni

Tissue specificityi

Intestine; expressed specifically in gut epithelium where it is not restricted to a particular cell lineage. Abundant expression is seen in the proximal colon with slightly lower levels in distal colon. Expression in the proximal colon is not restricted either to a particular cell lineage or stage of differentiation while in the distal colon it is more abundant in the differentiated cells towards the top of the crypt.

Gene expression databases

BgeeiP43241.
CleanExiMM_CDX2.
ExpressionAtlasiP43241. baseline and differential.
GenevestigatoriP43241.

Interactioni

Protein-protein interaction databases

BioGridi198664. 6 interactions.
STRINGi10090.ENSMUSP00000031650.

Structurei

3D structure databases

ProteinModelPortaliP43241.
SMRiP43241. Positions 189-242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi46 – 538Poly-Ala
Compositional biasi85 – 928Poly-Ala
Compositional biasi247 – 25711Poly-GlnAdd
BLAST

Sequence similaritiesi

Belongs to the Caudal homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG304047.
HOGENOMiHOG000115975.
HOVERGENiHBG005302.
InParanoidiP43241.
KOiK09312.
OMAiWMRKPTQ.
OrthoDBiEOG71P2BK.
PhylomeDBiP43241.
TreeFamiTF351605.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR006820. Caudal_activation_dom.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view]
PfamiPF04731. Caudal_act. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYVSYLLDKD VSMYPSSVRH SGGLNLAPQN FVSPPQYPDY GGYHVAAAAA
60 70 80 90 100
ATANLDSAQS PGPSWPTAYG APLREDWNGY APGGAAAANA VAHGLNGGSP
110 120 130 140 150
AAAMGYSSPA EYHAHHHPHH HPHHPAASPS CASGLLQTLN LGPPGPAATA
160 170 180 190 200
AAEQLSPSGQ RRNLCEWMRK PAQQSLGSQV KTRTKDKYRV VYTDHQRLEL
210 220 230 240 250
EKEFHFSRYI TIRRKSELAA TLGLSERQVK IWFQNRRAKE RKIKKKQQQQ
260 270 280 290 300
QQQQQQQPPQ PPPQPSQPQP GALRSVPEPL SPVTSLQGSV PGSVPGVLGP
310
AGGVLNSTVT Q
Length:311
Mass (Da):33,476
Last modified:November 1, 1995 - v1
Checksum:i71FFC4C263462FF3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti69 – 691Y → H in AAB32251 (PubMed:7935448).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00454 Unassigned DNA. Translation: AAA19645.1.
S74520 mRNA. Translation: AAB32251.1.
CCDSiCCDS19878.1.
PIRiA53808.
RefSeqiNP_031699.2. NM_007673.3.
UniGeneiMm.20358.

Genome annotation databases

EnsembliENSMUST00000031650; ENSMUSP00000031650; ENSMUSG00000029646.
GeneIDi12591.
KEGGimmu:12591.
UCSCiuc009anz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00454 Unassigned DNA. Translation: AAA19645.1.
S74520 mRNA. Translation: AAB32251.1.
CCDSiCCDS19878.1.
PIRiA53808.
RefSeqiNP_031699.2. NM_007673.3.
UniGeneiMm.20358.

3D structure databases

ProteinModelPortaliP43241.
SMRiP43241. Positions 189-242.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198664. 6 interactions.
STRINGi10090.ENSMUSP00000031650.

PTM databases

PhosphoSiteiP43241.

Proteomic databases

MaxQBiP43241.
PRIDEiP43241.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031650; ENSMUSP00000031650; ENSMUSG00000029646.
GeneIDi12591.
KEGGimmu:12591.
UCSCiuc009anz.1. mouse.

Organism-specific databases

CTDi1045.
MGIiMGI:88361. Cdx2.

Phylogenomic databases

eggNOGiNOG304047.
HOGENOMiHOG000115975.
HOVERGENiHBG005302.
InParanoidiP43241.
KOiK09312.
OMAiWMRKPTQ.
OrthoDBiEOG71P2BK.
PhylomeDBiP43241.
TreeFamiTF351605.

Enzyme and pathway databases

ReactomeiREACT_324802. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).

Miscellaneous databases

NextBioi281738.
PROiP43241.
SOURCEiSearch...

Gene expression databases

BgeeiP43241.
CleanExiMM_CDX2.
ExpressionAtlasiP43241. baseline and differential.
GenevestigatoriP43241.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR006820. Caudal_activation_dom.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view]
PfamiPF04731. Caudal_act. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structure of the murine homeobox gene cdx-2. Expression in embryonic and adult intestinal epithelium."
    James R.J., Erler T., Kazenwadel J.
    J. Biol. Chem. 269:15229-15237(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BALB/c.
  2. "A homeodomain protein related to caudal regulates intestine-specific gene transcription."
    Suh E., Chen L., Taylor J., Traber P.G.
    Mol. Cell. Biol. 14:7340-7351(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Small intestine.
  3. "Homeobox gene expression in the intestinal epithelium of adult mice."
    James R.J., Kazenwadel J.
    J. Biol. Chem. 266:3246-3251(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 204-229.
  4. "Phosphorylation of the serine 60 residue within the Cdx2 activation domain mediates its transactivation capacity."
    Rings E.H., Boudreau F., Taylor J.K., Moffett J., Suh E.R., Traber P.G.
    Gastroenterology 121:1437-1450(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-60.

Entry informationi

Entry nameiCDX2_MOUSE
AccessioniPrimary (citable) accession number: P43241
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 1, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.