Reviewed, UniProtKB/Swiss-Prot P43236 (CATK_RABIT)

Names and origin

Protein names

Recommended name:
    Cathepsin K
    EC=3.4.22.38
Alternative name(s):
    OC-2 protein

Gene names

Name:

CTSK

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
Catalytic activity	Broad proteolytic activity. With small-molecule substrates and inhibitors, the major determinant of specificity is P2, which is preferably Leu, Met > Phe, and not Arg.
Subcellular location	Lysosome By similarity.
Tissue specificity	Predominantly expressed in osteclasts (bones).
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	3D-structure
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 15

15

Potential

Propeptide

16 – 114

99

Activation peptide

PRO_0000026305

Chain

115 – 329

215

Cathepsin K

PRO_0000026306

Sites

Active site

139

1

By similarity

Active site

276

1

By similarity

Active site

296

1

By similarity

Amino acid modifications

Glycosylation

103

1

N-linked (GlcNAc...) Potential

Glycosylation

268

1

N-linked (GlcNAc...) Potential

Disulfide bond

136 ↔ 177

By similarity

Disulfide bond

170 ↔ 210

By similarity

Disulfide bond

269 ↔ 318

By similarity

Secondary structure

1

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329

Helix Strand Turn

Details...

Helix

121 – 124

4

Helix

139 – 156

18

Helix

164 – 170

7

Helix

176 – 178

3

Helix

182 – 192

11

Beta strand

195 – 197

3

Helix

198 – 200

3

Helix

214 – 216

3

Beta strand

217 – 219

3

Beta strand

224 – 226

3

Helix

232 – 241

10

Beta strand

245 – 249

5

Helix

254 – 257

4

Beta strand

261 – 264

4

Beta strand

276 – 286

11

Beta strand

289 – 295

7

Beta strand

307 – 316

10

Helix

317 – 319

3

Beta strand

325 – 327

3

Sequences

	Sequence		Length	Mass (Da)
	P43236-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 875D8582876EB51F	FASTA	329	36,870
	10 20 30 40 50 60 MWGLKVLLLP VVSFALHPEE ILDTQWELWK KTYSKQYNSK VDEISRRLIW EKNLKHISIH 70 80 90 100 110 120 NLEASLGVHT YELAMNHLGD MTSEEVVQKM TGLKVPPSRS HSNDTLYIPD WEGRTPDSID 130 140 150 160 170 180 YRKKGYVTPV KNQGQCGSCW AFSSVGALEG QLKKKTGKLL NLSPQNLVDC VSENYGCGGG 190 200 210 220 230 240 YMTNAFQYVQ RNRGIDSEDA YPYVGQDESC MYNPTGKAAK CRGYREIPEG NEKALKRAVA 250 260 270 280 290 300 RVGPVSVAID ASLTSFQFYS KGVYYDENCS SDNVNHAVLA VGYGIQKGNK HWIIKNSWGE 310 320 SWGNKGYILM ARNKNNACGI ANLASFPKM

References

[1]	"Molecular cloning of a possible cysteine proteinase predominantly expressed in osteoclasts." Tezuka K., Tezuka Y., Maejima A., Sato T., Nemoto K., Kamioka H., Hakeda Y., Kumegawa M. J. Biol. Chem. 269:1106-1109(1994) [PubMed: 8288568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Bone.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D14036 mRNA. Translation: BAA03125.1.

PIR

A49868.

RefSeq

NP_001076110.1.

UniGene

Ocu.6195

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2F7D	X-ray	1.90	A	115-329	[»]

SMR

P43236. Positions 20-329.

ModBase

Search...

Protein-protein interaction databases

STRING

P43236.

Protein family/group databases

MEROPS

I29.007.

Genome annotation databases

Ensembl

ENSOCUT00000011087; ENSOCUP00000009540; ENSOCUG00000011090; Oryctolagus cuniculus. [Genome view]

GeneID

100009334.

Organism-specific databases

CTD

100009334.

Phylogenomic databases

HOVERGEN

P43236.

Enzyme and pathway databases

BRENDA

3.4.22.38. 255.

Family and domain databases

InterPro

IPR000169. Pept_cys_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR015644. Peptidase_C1A_cathepsin-K.
IPR013201. Prot_inhib_I29.
[Graphical view]

PANTHER

PTHR12411:SF55. CathpsnK_like. 1 hit.
PTHR12411. Peptidase_C1A. 1 hit.

Pfam

PF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]

PRINTS

PR00705. PAPAIN.

ProDom

PD000158. Peptidase_C1. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00645. Pept_C1. 1 hit.
[Graphical view]

PROSITE

PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

BindingDB

P43236.

Entry information

Entry name

CATK_RABIT

Accession

Primary (citable) accession number: P43236

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 1, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)