Reviewed, UniProtKB/Swiss-Prot P43232 (CARP5_RHINI)

Names and origin

Protein names	Recommended name:     Rhizopuspepsin-5     EC=3.4.23.21 Alternative name(s):     Aspartate protease
Organism	Rhizopus niveus
Taxonomic identifier	4844 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Rhizopus

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of proteins with broad specificity similar to that of pepsin A, preferring hydrophobic residues at P1 and P1'. Clots milk and activates trypsinogen. Does not cleave 4-Gln-|-His-5, but does cleave 10-His-|-Leu-11 and 12-Val-|-Glu-13 in B chain of insulin.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 69	48	Activation peptide Potential		PRO_0000025891
	Chain	70 – 392	323	Rhizopuspepsin-5		PRO_0000025892
Sites
	Active site	103	1	By similarity
	Active site	286	1	By similarity
Amino acid modifications
	Disulfide bond	116 ↔ 119		By similarity
	Disulfide bond	320 ↔ 353		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43232-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 015579AA94B99EAE	FASTA	392	41,850
	10 20 30 40 50 60 MKFSLISSCV ALAVLVLSTE AAPNGKKVNI PLTKNKDYKP NAKNAIQKVL AKYHRHRSTS 70 80 90 100 110 120 SSSNSTSTDG IGRVPVTDYY NDIEYFGQVK VGTPGVTLKL DFDTGSSDLW FASSLCTNCG 130 140 150 160 170 180 YSQTKYNPNQ SRTYAKDGRA WSISYGDGSS ASGILGTDTV VLGGLTIQRQ TIELARREAS 190 200 210 220 230 240 SFQNGPSDGL LGLGFNSITT VRGVKTPVDN LISQGLISNP VFGVYLGKES NGGGGEYIFG 250 260 270 280 290 300 GYDSSKFKGS LTTIPVDNSN GWYGVTIRGA SIGRSRVAGS FEAILDTGTS LLVLPNDVAR 310 320 330 340 350 360 SVASAYGARD NYDGTFSISC DTSRFQPLVF TIGSSTFEVP ADSLVYEQNG YSCIAGFGYG 370 380 390 DYDFAIFDDV FLKNNYVVFN PTVPQVQIAT VA

References

[1]	Horiuchi H., Nakamura H., Okazaki T., Yano K., Takagi M. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IFO 4810 / AS 3.4817.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X56993 Genomic DNA. Translation: CAA40310.1.
3D structure databases
HSSP	HSSP built from PDB template 2APR based on UniProtKB P06026.
SMR	P43232. Positions 70-392.
ModBase	Search...
Protein family/group databases
MEROPS	A01.012.
Enzyme and pathway databases
BRENDA	3.4.23.21. 18853.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARP5_RHINI

Accession

Primary (citable) accession number: P43232

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)