P43219 (GIPR_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gastric inhibitory polypeptide receptor
Short name=GIP-R

Alternative name(s):
Glucose-dependent insulinotropic polypeptide receptor

Gene names

Name:

Gipr

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is a receptor for GIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Subunit structure	May form homodimers and heterodimers with GLP1R By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Present in the pancreas as well as the gut, adipose tissue, heart, pituitary, and inner layers of the adrenal cortex, whereas it is not found in kidney, spleen, or liver. It is also expressed in several brain regions, including the cerebral cortex, hippocampus, and olfactory bulb.
Post-translational modification	N-glycosylation is required for cell surface expression and lengthens receptor half-life by preventing degradation in the ER By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 2 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	desensitization of G-protein coupled receptor protein signaling pathway Inferred from direct assay PubMed 8928774. Source: RGD elevation of cytosolic calcium ion concentration Inferred from direct assay Ref.1. Source: RGD endocrine pancreas development Inferred from electronic annotation. Source: Compara positive regulation of cAMP biosynthetic process Inferred from mutant phenotype PubMed 11014226. Source: RGD positive regulation of insulin secretion Inferred from mutant phenotype PubMed 11014226. Source: RGD response to axon injury Inferred from expression pattern PubMed 19170165. Source: RGD response to calcium ion Inferred from mutant phenotype PubMed 9705086. Source: RGD response to fatty acid Inferred from expression pattern PubMed 12475913. Source: RGD response to glucose stimulus Inferred from expression pattern PubMed 12475913. Source: RGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 19170165. Source: RGD
Molecular_function	gastric inhibitory peptide receptor activity Inferred from direct assay PubMed 8958204. Source: RGD peptide hormone binding Inferred from physical interaction PubMed 8958204. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 455	437	Gastric inhibitory polypeptide receptor		PRO_0000012827
Regions
	Topological domain	19 – 135	117	Extracellular Potential
	Transmembrane	136 – 158	23	Helical; Name=1; Potential
	Topological domain	159 – 166	8	Cytoplasmic Potential
	Transmembrane	167 – 186	20	Helical; Name=2; Potential
	Topological domain	187 – 214	28	Extracellular Potential
	Transmembrane	215 – 239	25	Helical; Name=3; Potential
	Topological domain	240 – 251	12	Cytoplasmic Potential
	Transmembrane	252 – 275	24	Helical; Name=4; Potential
	Topological domain	276 – 290	15	Extracellular Potential
	Transmembrane	291 – 316	26	Helical; Name=5; Potential
	Topological domain	317 – 338	22	Cytoplasmic Potential
	Transmembrane	339 – 359	21	Helical; Name=6; Potential
	Topological domain	360 – 374	15	Extracellular Potential
	Transmembrane	375 – 395	21	Helical; Name=7; Potential
	Topological domain	396 – 455	60	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	59	1	N-linked (GlcNAc...) Potential
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Glycosylation	74	1	N-linked (GlcNAc...) Potential
	Disulfide bond	43 ↔ 67		By similarity
	Disulfide bond	58 ↔ 100		By similarity
	Disulfide bond	81 ↔ 115		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43219 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 5454B0638ABF9A06	FASTA	455	52,257
	10 20 30 40 50 60 MPLRLLLLLL WLWGLSLQRA ETDSEGQTTG ELYQRWERYG WECQNTLEAT EPPSGLACNG 70 80 90 100 110 120 SFDMYACWNY TAANTTARVS CPWYLPWYRQ VAAGFVFRQC GSDGQWGSWR DHTQCENPEK 130 140 150 160 170 180 NGAFQDQKLI LERLQVVYTV GYSLSLATLL LALLILSLFR RLHCTRNYIH MNLFTSFMLR 190 200 210 220 230 240 AGAILTRDQL LPPLGPYTGN QTPTLWNQAL AACRTAQILT QYCVGANYTW LLVEGVYLHH 250 260 270 280 290 300 LLVVVRRSEK GHFRCYLLLG WGAPALFVIP WVIVRYLYEN TQCWERNEVK AIWWIIRTPI 310 320 330 340 350 360 LITILINFLI FIRILGILVS KLRTRQMRCP DYRLRLARST LTLMPLLGVH EVVFAPVTEE 370 380 390 400 410 420 QAEGSLRFAK LAFEIFLSSF QGFLVSVLYC FINKEVQSEI RRLRLSLQEQ CPRPHLGQAP 430 440 450 RAVPLSSAPQ EAAIRNALPS GMLHVPGDEV LESYC

References

[1]	"Gastric inhibitory polypeptide receptor, a member of the secretin-vasoactive intestinal peptide receptor family, is widely distributed in peripheral organs and the brain." Usdin T.B., Mezey E., Button D.C., Brownstein M.J., Bonner T.I. Endocrinology 133:2861-2871(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19660 mRNA. Translation: AAC37637.1.
IPI	IPI00198412.
PIR	I53273.
RefSeq	NP_036846.1. NM_012714.1.
UniGene	Rn.9676.
3D structure databases
ProteinModelPortal	P43219.
SMR	P43219. Positions 26-119.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000021456.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	P43219.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000021456; ENSRNOP00000021456; ENSRNOG00000015860.
GeneID	25024.
KEGG	rno:25024.
UCSC	RGD:2689. rat.
Organism-specific databases
CTD	2696.
RGD	2689. Gipr.
Phylogenomic databases
eggNOG	NOG239460.
GeneTree	ENSGT00580000081498.
HOGENOM	HOG000008250.
HOVERGEN	HBG008318.
InParanoid	P43219.
KO	K04580.
OMA	TRQMRCP.
OrthoDB	EOG469QTS.
Gene expression databases
Genevestigator	P43219.
GermOnline	ENSRNOG00000015860. Rattus norvegicus.
Family and domain databases
InterPro	IPR017981. GPCR_2-like. IPR001879. GPCR_2_extracellular_dom. IPR001749. GPCR_2_GIP_rcpt. IPR000832. GPCR_2_secretin-like. IPR017983. GPCR_2_secretin-like_CS. [Graphical view]
Pfam	PF00002. 7tm_2. 1 hit. PF02793. HRM. 1 hit. [Graphical view]
PRINTS	PR01129. GIPRECEPTOR. PR00249. GPCRSECRETIN.
SMART	SM00008. HormR. 1 hit. [Graphical view]
PROSITE	PS00649. G_PROTEIN_RECEP_F2_1. 1 hit. PS00650. G_PROTEIN_RECEP_F2_2. 1 hit. PS50227. G_PROTEIN_RECEP_F2_3. 1 hit. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P43219.
ChEMBL	CHEMBL5001.
NextBio	605153.

Entry information

Entry name

GIPR_RAT

Accession

Primary (citable) accession number: P43219

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program