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P43212 (PGLR2_CRYJA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 28, 2011. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Polygalacturonase
Short name=PG
EC=3.2.1.15
Alternative name(s):
Allergen Cry j II
Major pollen allergen Cry j 2
Pectinase
Allergen=Cry j 2
OrganismCryptomeria japonica (Japanese cedar) (Cupressus japonica)
Taxonomic identifier3369 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Coniferopsida › Coniferales › Cupressaceae › Cryptomeria

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Subcellular location

Secreted Potential. Plastid › amyloplast Potential. Secreted › cell wall Potential.

Allergenic properties

Causes an allergic reaction in human.

Sequence similarities

Belongs to the glycosyl hydrolase 28 family.

Contains 5 PbH1 repeats.

Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
Fruit ripening
   Cellular componentAmyloplast
Cell wall
Plastid
Secreted
   DiseaseAllergen
   DomainRepeat
Signal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Zymogen
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

cellular cell wall organization

Inferred from electronic annotation. Source: UniProtKB-KW

ripening

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentamyloplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionpolygalacturonase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Propeptide23 – 4523 Potential
PRO_0000024816
Chain46 – 433388Polygalacturonase
PRO_0000024817
Propeptide434 – 51481 Potential
PRO_0000024818

Regions

Repeat214 – 24027PbH1 1
Repeat241 – 26222PbH1 2
Repeat264 – 28421PbH1 3
Repeat294 – 31522PbH1 4
Repeat323 – 34422PbH1 5

Sites

Active site2551Proton donor By similarity
Active site2781 By similarity

Amino acid modifications

Glycosylation4601N-linked (GlcNAc...) Potential
Glycosylation4721N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict51F → L in BAA06172. Ref.2
Sequence conflict121V → L in BAA06172. Ref.2
Sequence conflict34 – 352DI → VV Ref.2
Sequence conflict371Q → K Ref.2
Sequence conflict881K → N in BAA06172. Ref.2
Sequence conflict981N → S in BAA06172. Ref.2
Sequence conflict4511K → E in BAA06172. Ref.2
Sequence conflict4541G → R in BAA06172. Ref.2
Sequence conflict5041M → I in BAA06172. Ref.2
Sequence conflict5071R → C in BAA06172. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P43212 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 624611C3FA8D6302

FASTA51456,645
        10         20         30         40         50         60 
MAMKFIAPMA FVAMQLIIMA AAEDQSAQIM LDSDIEQYLR SNRSLRKVEH SRHDAINIFN 

        70         80         90        100        110        120 
VEKYGAVGDG KHDCTEAFST AWQAACKKPS AMLLVPGNKK FVVNNLFFNG PCQPHFTFKV 

       130        140        150        160        170        180 
DGIIAAYQNP ASWKNNRIWL QFAKLTGFTL MGKGVIDGQG KQWWAGQCKW VNGREICNDR 

       190        200        210        220        230        240 
DRPTAIKFDF STGLIIQGLK LMNSPEFHLV FGNCEGVKII GISITAPRDS PNTDGIDIFA 

       250        260        270        280        290        300 
SKNFHLQKNT IGTGDDCVAI GTGSSNIVIE DLICGPGHGI SIGSLGRENS RAEVSYVHVN 

       310        320        330        340        350        360 
GAKFIDTQNG LRIKTWQGGS GMASHIIYEN VEMINSENPI LINQFYCTSA SACQNQRSAV 

       370        380        390        400        410        420 
QIQDVTYKNI RGTSATAAAI QLKCSDSMPC KDIKLSDISL KLTSGKIASC LNDNANGYFS 

       430        440        450        460        470        480 
GHVIPACKNL SPSAKRKESK SHKHPKTVMV KNMGAYDKGN RTRILLGSRP PNCTNKCHGC 

       490        500        510 
SPCKAKLVIV HRIMPQEYYP QRWMCSRHGK IYHP

« Hide

References

[1]"Molecular cloning of the second major allergen, Cry j II, from Japanese cedar pollen."
Namba M., Kurose M., Torigoe K., Hino K., Taniguchi Y., Fukuda S., Usui M., Kurimoto M.
FEBS Lett. 353:124-128(1994) [PubMed: 7926035] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Pollen.
[2]"cDNA cloning and expression of Cry j II the second major allergen of Japanese cedar pollen."
Komiyama N., Sone T., Shimizu K., Morikubo K., Kino K.
Biochem. Biophys. Res. Commun. 201:1021-1028(1994) [PubMed: 8002972] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pollen.
[3]"Identification of the second major allergen of Japanese cedar pollen."
Sakaguchi M., Inouye S., Taniai M., Ando S., Usui M., Matuhasi T.
Allergy 45:309-312(1990) [PubMed: 2382797] [Abstract]
Cited for: PROTEIN SEQUENCE OF 55-64.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D37765 mRNA. Translation: BAA07021.1.
D29772 mRNA. Translation: BAA06172.1.
PIRJC2498.
S48730.

3D structure databases

ProteinModelPortalP43212.
ModBaseSearch...

Protein family/group databases

Allergome249. Cry j 2.
250. Cry j 2.0101.
251. Cry j 2.0102.
CAZyGH28. Glycoside Hydrolase Family 28.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
PfamPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMSSF51126. Pectin_lyas_like. 1 hit.
PROSITEPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGLR2_CRYJA
AccessionPrimary (citable) accession number: P43212
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 28, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Allergens

Nomenclature of allergens and list of entries

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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