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P43212

- PGLR2_CRYJA

UniProt

P43212 - PGLR2_CRYJA

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Protein
Polygalacturonase
Gene
N/A
Organism
Cryptomeria japonica (Japanese cedar) (Cupressus japonica)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei255 – 2551Proton donor By similarity
Active sitei278 – 2781 By similarity

GO - Molecular functioni

  1. polygalacturonase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. fruit ripening Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Fruit ripening

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Polygalacturonase (EC:3.2.1.15)
Short name:
PG
Alternative name(s):
Allergen Cry j II
Major pollen allergen Cry j 2
Pectinase
Allergen: Cry j 2
OrganismiCryptomeria japonica (Japanese cedar) (Cupressus japonica)
Taxonomic identifieri3369 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesCupressaceaeCryptomeria

Subcellular locationi

Secreted Reviewed prediction. Plastidamyloplast Reviewed prediction. Secretedcell wall Reviewed prediction

GO - Cellular componenti

  1. amyloplast Source: UniProtKB-SubCell
  2. cell wall Source: UniProtKB-SubCell
  3. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Cell wall, Plastid, Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei249. Cry j 2.
250. Cry j 2.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed prediction
Add
BLAST
Propeptidei23 – 4523 Reviewed prediction
PRO_0000024816Add
BLAST
Chaini46 – 433388Polygalacturonase
PRO_0000024817Add
BLAST
Propeptidei434 – 51481 Reviewed prediction
PRO_0000024818Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi460 – 4601N-linked (GlcNAc...) Reviewed prediction
Glycosylationi472 – 4721N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP43212.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati214 – 24027PbH1 1
Add
BLAST
Repeati241 – 26222PbH1 2
Add
BLAST
Repeati264 – 28421PbH1 3
Add
BLAST
Repeati294 – 31522PbH1 4
Add
BLAST
Repeati323 – 34422PbH1 5
Add
BLAST

Sequence similaritiesi

Contains 5 PbH1 repeats.

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43212-1 [UniParc]FASTAAdd to Basket

« Hide

MAMKFIAPMA FVAMQLIIMA AAEDQSAQIM LDSDIEQYLR SNRSLRKVEH    50
SRHDAINIFN VEKYGAVGDG KHDCTEAFST AWQAACKKPS AMLLVPGNKK 100
FVVNNLFFNG PCQPHFTFKV DGIIAAYQNP ASWKNNRIWL QFAKLTGFTL 150
MGKGVIDGQG KQWWAGQCKW VNGREICNDR DRPTAIKFDF STGLIIQGLK 200
LMNSPEFHLV FGNCEGVKII GISITAPRDS PNTDGIDIFA SKNFHLQKNT 250
IGTGDDCVAI GTGSSNIVIE DLICGPGHGI SIGSLGRENS RAEVSYVHVN 300
GAKFIDTQNG LRIKTWQGGS GMASHIIYEN VEMINSENPI LINQFYCTSA 350
SACQNQRSAV QIQDVTYKNI RGTSATAAAI QLKCSDSMPC KDIKLSDISL 400
KLTSGKIASC LNDNANGYFS GHVIPACKNL SPSAKRKESK SHKHPKTVMV 450
KNMGAYDKGN RTRILLGSRP PNCTNKCHGC SPCKAKLVIV HRIMPQEYYP 500
QRWMCSRHGK IYHP 514
Length:514
Mass (Da):56,645
Last modified:November 1, 1995 - v1
Checksum:i624611C3FA8D6302
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51F → L in BAA06172. 1 Publication
Sequence conflicti12 – 121V → L in BAA06172. 1 Publication
Sequence conflicti34 – 352DI → VV1 Publication
Sequence conflicti37 – 371Q → K1 Publication
Sequence conflicti88 – 881K → N in BAA06172. 1 Publication
Sequence conflicti98 – 981N → S in BAA06172. 1 Publication
Sequence conflicti451 – 4511K → E in BAA06172. 1 Publication
Sequence conflicti454 – 4541G → R in BAA06172. 1 Publication
Sequence conflicti504 – 5041M → I in BAA06172. 1 Publication
Sequence conflicti507 – 5071R → C in BAA06172. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D37765 mRNA. Translation: BAA07021.1.
D29772 mRNA. Translation: BAA06172.1.
PIRiJC2498.
S48730.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D37765 mRNA. Translation: BAA07021.1 .
D29772 mRNA. Translation: BAA06172.1 .
PIRi JC2498.
S48730.

3D structure databases

ProteinModelPortali P43212.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 249. Cry j 2.
250. Cry j 2.0101.
CAZyi GH28. Glycoside Hydrolase Family 28.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.160.20.10. 1 hit.
InterProi IPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view ]
Pfami PF00295. Glyco_hydro_28. 1 hit.
[Graphical view ]
SMARTi SM00710. PbH1. 5 hits.
[Graphical view ]
SUPFAMi SSF51126. SSF51126. 1 hit.
PROSITEi PS00502. POLYGALACTURONASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of the second major allergen, Cry j II, from Japanese cedar pollen."
    Namba M., Kurose M., Torigoe K., Hino K., Taniguchi Y., Fukuda S., Usui M., Kurimoto M.
    FEBS Lett. 353:124-128(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Pollen.
  2. "cDNA cloning and expression of Cry j II the second major allergen of Japanese cedar pollen."
    Komiyama N., Sone T., Shimizu K., Morikubo K., Kino K.
    Biochem. Biophys. Res. Commun. 201:1021-1028(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pollen.
  3. "Identification of the second major allergen of Japanese cedar pollen."
    Sakaguchi M., Inouye S., Taniai M., Ando S., Usui M., Matuhasi T.
    Allergy 45:309-312(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 55-64.

Entry informationi

Entry nameiPGLR2_CRYJA
AccessioniPrimary (citable) accession number: P43212
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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