Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P43212

- PGLR2_CRYJA

UniProt

P43212 - PGLR2_CRYJA

Protein

Polygalacturonase

Gene
N/A
Organism
Cryptomeria japonica (Japanese cedar) (Cupressus japonica)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei255 – 2551Proton donorPROSITE-ProRule annotation
    Active sitei278 – 2781PROSITE-ProRule annotation

    GO - Molecular functioni

    1. polygalacturonase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. fruit ripening Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation, Fruit ripening

    Protein family/group databases

    CAZyiGH28. Glycoside Hydrolase Family 28.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polygalacturonase (EC:3.2.1.15)
    Short name:
    PG
    Alternative name(s):
    Allergen Cry j II
    Major pollen allergen Cry j 2
    Pectinase
    Allergen: Cry j 2
    OrganismiCryptomeria japonica (Japanese cedar) (Cupressus japonica)
    Taxonomic identifieri3369 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesCupressaceaeCryptomeria

    Subcellular locationi

    Secreted Curated. Plastidamyloplast Curated. Secretedcell wall Curated

    GO - Cellular componenti

    1. amyloplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell
    3. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Amyloplast, Cell wall, Plastid, Secreted

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human.

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei249. Cry j 2.
    250. Cry j 2.0101.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Propeptidei23 – 4523Sequence AnalysisPRO_0000024816Add
    BLAST
    Chaini46 – 433388PolygalacturonasePRO_0000024817Add
    BLAST
    Propeptidei434 – 51481Sequence AnalysisPRO_0000024818Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi460 – 4601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi472 – 4721N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Zymogen

    Structurei

    3D structure databases

    ProteinModelPortaliP43212.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati214 – 24027PbH1 1Add
    BLAST
    Repeati241 – 26222PbH1 2Add
    BLAST
    Repeati264 – 28421PbH1 3Add
    BLAST
    Repeati294 – 31522PbH1 4Add
    BLAST
    Repeati323 – 34422PbH1 5Add
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 28 family.Curated
    Contains 5 PbH1 repeats.Curated

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di2.160.20.10. 1 hit.
    InterProiIPR000743. Glyco_hydro_28.
    IPR006626. PbH1.
    IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view]
    PfamiPF00295. Glyco_hydro_28. 1 hit.
    [Graphical view]
    SMARTiSM00710. PbH1. 5 hits.
    [Graphical view]
    SUPFAMiSSF51126. SSF51126. 1 hit.
    PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43212-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAMKFIAPMA FVAMQLIIMA AAEDQSAQIM LDSDIEQYLR SNRSLRKVEH    50
    SRHDAINIFN VEKYGAVGDG KHDCTEAFST AWQAACKKPS AMLLVPGNKK 100
    FVVNNLFFNG PCQPHFTFKV DGIIAAYQNP ASWKNNRIWL QFAKLTGFTL 150
    MGKGVIDGQG KQWWAGQCKW VNGREICNDR DRPTAIKFDF STGLIIQGLK 200
    LMNSPEFHLV FGNCEGVKII GISITAPRDS PNTDGIDIFA SKNFHLQKNT 250
    IGTGDDCVAI GTGSSNIVIE DLICGPGHGI SIGSLGRENS RAEVSYVHVN 300
    GAKFIDTQNG LRIKTWQGGS GMASHIIYEN VEMINSENPI LINQFYCTSA 350
    SACQNQRSAV QIQDVTYKNI RGTSATAAAI QLKCSDSMPC KDIKLSDISL 400
    KLTSGKIASC LNDNANGYFS GHVIPACKNL SPSAKRKESK SHKHPKTVMV 450
    KNMGAYDKGN RTRILLGSRP PNCTNKCHGC SPCKAKLVIV HRIMPQEYYP 500
    QRWMCSRHGK IYHP 514
    Length:514
    Mass (Da):56,645
    Last modified:November 1, 1995 - v1
    Checksum:i624611C3FA8D6302
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51F → L in BAA06172. (PubMed:8002972)Curated
    Sequence conflicti12 – 121V → L in BAA06172. (PubMed:8002972)Curated
    Sequence conflicti34 – 352DI → VV(PubMed:8002972)Curated
    Sequence conflicti37 – 371Q → K(PubMed:8002972)Curated
    Sequence conflicti88 – 881K → N in BAA06172. (PubMed:8002972)Curated
    Sequence conflicti98 – 981N → S in BAA06172. (PubMed:8002972)Curated
    Sequence conflicti451 – 4511K → E in BAA06172. (PubMed:8002972)Curated
    Sequence conflicti454 – 4541G → R in BAA06172. (PubMed:8002972)Curated
    Sequence conflicti504 – 5041M → I in BAA06172. (PubMed:8002972)Curated
    Sequence conflicti507 – 5071R → C in BAA06172. (PubMed:8002972)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D37765 mRNA. Translation: BAA07021.1.
    D29772 mRNA. Translation: BAA06172.1.
    PIRiJC2498.
    S48730.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D37765 mRNA. Translation: BAA07021.1 .
    D29772 mRNA. Translation: BAA06172.1 .
    PIRi JC2498.
    S48730.

    3D structure databases

    ProteinModelPortali P43212.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 249. Cry j 2.
    250. Cry j 2.0101.
    CAZyi GH28. Glycoside Hydrolase Family 28.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.160.20.10. 1 hit.
    InterProi IPR000743. Glyco_hydro_28.
    IPR006626. PbH1.
    IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view ]
    Pfami PF00295. Glyco_hydro_28. 1 hit.
    [Graphical view ]
    SMARTi SM00710. PbH1. 5 hits.
    [Graphical view ]
    SUPFAMi SSF51126. SSF51126. 1 hit.
    PROSITEi PS00502. POLYGALACTURONASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the second major allergen, Cry j II, from Japanese cedar pollen."
      Namba M., Kurose M., Torigoe K., Hino K., Taniguchi Y., Fukuda S., Usui M., Kurimoto M.
      FEBS Lett. 353:124-128(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Pollen.
    2. "cDNA cloning and expression of Cry j II the second major allergen of Japanese cedar pollen."
      Komiyama N., Sone T., Shimizu K., Morikubo K., Kino K.
      Biochem. Biophys. Res. Commun. 201:1021-1028(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pollen.
    3. "Identification of the second major allergen of Japanese cedar pollen."
      Sakaguchi M., Inouye S., Taniai M., Ando S., Usui M., Matuhasi T.
      Allergy 45:309-312(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 55-64.

    Entry informationi

    Entry nameiPGLR2_CRYJA
    AccessioniPrimary (citable) accession number: P43212
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3