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Protein

Polygalacturonase

Gene
N/A
Organism
Cryptomeria japonica (Japanese cedar) (Cupressus japonica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei255Proton donorPROSITE-ProRule annotation1
Active sitei278PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Fruit ripening

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Polygalacturonase (EC:3.2.1.15)
Short name:
PG
Alternative name(s):
Allergen Cry j II
Major pollen allergen Cry j 2
Pectinase
Allergen: Cry j 2
OrganismiCryptomeria japonica (Japanese cedar) (Cupressus japonica)
Taxonomic identifieri3369 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesCupressaceaeCryptomeria

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Cell wall, Plastid, Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei249. Cry j 2.
250. Cry j 2.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000002481623 – 45Sequence analysisAdd BLAST23
ChainiPRO_000002481746 – 433PolygalacturonaseAdd BLAST388
PropeptideiPRO_0000024818434 – 514Sequence analysisAdd BLAST81

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi460N-linked (GlcNAc...)Sequence analysis1
Glycosylationi472N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP43212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati214 – 240PbH1 1Add BLAST27
Repeati241 – 262PbH1 2Add BLAST22
Repeati264 – 284PbH1 3Add BLAST21
Repeati294 – 315PbH1 4Add BLAST22
Repeati323 – 344PbH1 5Add BLAST22

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 5 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43212-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMKFIAPMA FVAMQLIIMA AAEDQSAQIM LDSDIEQYLR SNRSLRKVEH
60 70 80 90 100
SRHDAINIFN VEKYGAVGDG KHDCTEAFST AWQAACKKPS AMLLVPGNKK
110 120 130 140 150
FVVNNLFFNG PCQPHFTFKV DGIIAAYQNP ASWKNNRIWL QFAKLTGFTL
160 170 180 190 200
MGKGVIDGQG KQWWAGQCKW VNGREICNDR DRPTAIKFDF STGLIIQGLK
210 220 230 240 250
LMNSPEFHLV FGNCEGVKII GISITAPRDS PNTDGIDIFA SKNFHLQKNT
260 270 280 290 300
IGTGDDCVAI GTGSSNIVIE DLICGPGHGI SIGSLGRENS RAEVSYVHVN
310 320 330 340 350
GAKFIDTQNG LRIKTWQGGS GMASHIIYEN VEMINSENPI LINQFYCTSA
360 370 380 390 400
SACQNQRSAV QIQDVTYKNI RGTSATAAAI QLKCSDSMPC KDIKLSDISL
410 420 430 440 450
KLTSGKIASC LNDNANGYFS GHVIPACKNL SPSAKRKESK SHKHPKTVMV
460 470 480 490 500
KNMGAYDKGN RTRILLGSRP PNCTNKCHGC SPCKAKLVIV HRIMPQEYYP
510
QRWMCSRHGK IYHP
Length:514
Mass (Da):56,645
Last modified:November 1, 1995 - v1
Checksum:i624611C3FA8D6302
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5F → L in BAA06172 (PubMed:8002972).Curated1
Sequence conflicti12V → L in BAA06172 (PubMed:8002972).Curated1
Sequence conflicti34 – 35DI → VV (PubMed:8002972).Curated2
Sequence conflicti37Q → K (PubMed:8002972).Curated1
Sequence conflicti88K → N in BAA06172 (PubMed:8002972).Curated1
Sequence conflicti98N → S in BAA06172 (PubMed:8002972).Curated1
Sequence conflicti451K → E in BAA06172 (PubMed:8002972).Curated1
Sequence conflicti454G → R in BAA06172 (PubMed:8002972).Curated1
Sequence conflicti504M → I in BAA06172 (PubMed:8002972).Curated1
Sequence conflicti507R → C in BAA06172 (PubMed:8002972).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37765 mRNA. Translation: BAA07021.1.
D29772 mRNA. Translation: BAA06172.1.
PIRiJC2498.
S48730.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37765 mRNA. Translation: BAA07021.1.
D29772 mRNA. Translation: BAA06172.1.
PIRiJC2498.
S48730.

3D structure databases

ProteinModelPortaliP43212.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei249. Cry j 2.
250. Cry j 2.0101.
CAZyiGH28. Glycoside Hydrolase Family 28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGLR2_CRYJA
AccessioniPrimary (citable) accession number: P43212
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.