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P43212

- PGLR2_CRYJA

UniProt

P43212 - PGLR2_CRYJA

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Protein

Polygalacturonase

Gene
N/A
Organism
Cryptomeria japonica (Japanese cedar) (Cupressus japonica)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei255 – 2551Proton donorPROSITE-ProRule annotation
Active sitei278 – 2781PROSITE-ProRule annotation

GO - Molecular functioni

  1. polygalacturonase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell wall organization Source: UniProtKB-KW
  3. fruit ripening Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Fruit ripening

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Polygalacturonase (EC:3.2.1.15)
Short name:
PG
Alternative name(s):
Allergen Cry j II
Major pollen allergen Cry j 2
Pectinase
Allergen: Cry j 2
OrganismiCryptomeria japonica (Japanese cedar) (Cupressus japonica)
Taxonomic identifieri3369 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesCupressaceaeCryptomeria

Subcellular locationi

Secreted Curated. Plastidamyloplast Curated. Secretedcell wall Curated

GO - Cellular componenti

  1. amyloplast Source: UniProtKB-KW
  2. cell wall Source: UniProtKB-KW
  3. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Cell wall, Plastid, Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei249. Cry j 2.
250. Cry j 2.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Propeptidei23 – 4523Sequence AnalysisPRO_0000024816Add
BLAST
Chaini46 – 433388PolygalacturonasePRO_0000024817Add
BLAST
Propeptidei434 – 51481Sequence AnalysisPRO_0000024818Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi460 – 4601N-linked (GlcNAc...)Sequence Analysis
Glycosylationi472 – 4721N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP43212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati214 – 24027PbH1 1Add
BLAST
Repeati241 – 26222PbH1 2Add
BLAST
Repeati264 – 28421PbH1 3Add
BLAST
Repeati294 – 31522PbH1 4Add
BLAST
Repeati323 – 34422PbH1 5Add
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 5 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43212-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAMKFIAPMA FVAMQLIIMA AAEDQSAQIM LDSDIEQYLR SNRSLRKVEH
60 70 80 90 100
SRHDAINIFN VEKYGAVGDG KHDCTEAFST AWQAACKKPS AMLLVPGNKK
110 120 130 140 150
FVVNNLFFNG PCQPHFTFKV DGIIAAYQNP ASWKNNRIWL QFAKLTGFTL
160 170 180 190 200
MGKGVIDGQG KQWWAGQCKW VNGREICNDR DRPTAIKFDF STGLIIQGLK
210 220 230 240 250
LMNSPEFHLV FGNCEGVKII GISITAPRDS PNTDGIDIFA SKNFHLQKNT
260 270 280 290 300
IGTGDDCVAI GTGSSNIVIE DLICGPGHGI SIGSLGRENS RAEVSYVHVN
310 320 330 340 350
GAKFIDTQNG LRIKTWQGGS GMASHIIYEN VEMINSENPI LINQFYCTSA
360 370 380 390 400
SACQNQRSAV QIQDVTYKNI RGTSATAAAI QLKCSDSMPC KDIKLSDISL
410 420 430 440 450
KLTSGKIASC LNDNANGYFS GHVIPACKNL SPSAKRKESK SHKHPKTVMV
460 470 480 490 500
KNMGAYDKGN RTRILLGSRP PNCTNKCHGC SPCKAKLVIV HRIMPQEYYP
510
QRWMCSRHGK IYHP
Length:514
Mass (Da):56,645
Last modified:November 1, 1995 - v1
Checksum:i624611C3FA8D6302
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51F → L in BAA06172. (PubMed:8002972)Curated
Sequence conflicti12 – 121V → L in BAA06172. (PubMed:8002972)Curated
Sequence conflicti34 – 352DI → VV(PubMed:8002972)Curated
Sequence conflicti37 – 371Q → K(PubMed:8002972)Curated
Sequence conflicti88 – 881K → N in BAA06172. (PubMed:8002972)Curated
Sequence conflicti98 – 981N → S in BAA06172. (PubMed:8002972)Curated
Sequence conflicti451 – 4511K → E in BAA06172. (PubMed:8002972)Curated
Sequence conflicti454 – 4541G → R in BAA06172. (PubMed:8002972)Curated
Sequence conflicti504 – 5041M → I in BAA06172. (PubMed:8002972)Curated
Sequence conflicti507 – 5071R → C in BAA06172. (PubMed:8002972)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37765 mRNA. Translation: BAA07021.1.
D29772 mRNA. Translation: BAA06172.1.
PIRiJC2498.
S48730.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37765 mRNA. Translation: BAA07021.1 .
D29772 mRNA. Translation: BAA06172.1 .
PIRi JC2498.
S48730.

3D structure databases

ProteinModelPortali P43212.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 249. Cry j 2.
250. Cry j 2.0101.
CAZyi GH28. Glycoside Hydrolase Family 28.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.160.20.10. 1 hit.
InterProi IPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view ]
Pfami PF00295. Glyco_hydro_28. 1 hit.
[Graphical view ]
SMARTi SM00710. PbH1. 5 hits.
[Graphical view ]
SUPFAMi SSF51126. SSF51126. 1 hit.
PROSITEi PS00502. POLYGALACTURONASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of the second major allergen, Cry j II, from Japanese cedar pollen."
    Namba M., Kurose M., Torigoe K., Hino K., Taniguchi Y., Fukuda S., Usui M., Kurimoto M.
    FEBS Lett. 353:124-128(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Pollen.
  2. "cDNA cloning and expression of Cry j II the second major allergen of Japanese cedar pollen."
    Komiyama N., Sone T., Shimizu K., Morikubo K., Kino K.
    Biochem. Biophys. Res. Commun. 201:1021-1028(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pollen.
  3. "Identification of the second major allergen of Japanese cedar pollen."
    Sakaguchi M., Inouye S., Taniai M., Ando S., Usui M., Matuhasi T.
    Allergy 45:309-312(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 55-64.

Entry informationi

Entry nameiPGLR2_CRYJA
AccessioniPrimary (citable) accession number: P43212
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3