Reviewed, UniProtKB/Swiss-Prot P43210 (HEM2_SOYBN)

Names and origin

Protein names

Recommended name:
    Delta-aminolevulinic acid dehydratase, chloroplastic
      Short name=ALADH
    EC=4.2.1.24
Alternative name(s):
    Porphobilinogen synthase

Gene names

Name:	HEMB
Synonyms:	ALAD

Organism

Glycine max (Soybean)

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Is committed to plant tetrapyrrole synthesis.
Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Cofactor	Magnesium.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Subcellular location	Plastid › chloroplast.
Tissue specificity	Leaves and root nodules.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Magnesium
Molecular function	Lyase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 48	48	Chloroplast Potential
	Chain	49 – 412	364	Delta-aminolevulinic acid dehydratase, chloroplastic		PRO_0000013320
Regions
	Region	201 – 219	19	Magnesium-binding By similarity
Sites
	Active site	333	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43210-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 0ECDF209A09572D8	FASTA	412	45,068
	10 20 30 40 50 60 MASSIPNAPS AFNSQSYVGL RAPLRTFNFS SPQAAKIPRS QRLFVVRASD SEFEAAVVAG 70 80 90 100 110 120 KVPPAPPVRP RPAAPVGTPV VPSLPLHRRP RRNRKSPALR SAFQETSISP ANFVYPLFIH 130 140 150 160 170 180 EGEEDTPIGA MPGCYRLGWR HGLVEEVAKA RDVGVNSVVL FPKIPDALKS PTGDEAYNEN 190 200 210 220 230 240 GLVPRTIRLL KDKYPDLVIY TDVALDPYSS DGHDGIVRED GVIMNDETVH QLCKQAVAQA 250 260 270 280 290 300 QAGADVVSPS DMMDGRVGAL RAALDAEGFQ HVSIMSYTAK YASSFYGPFR EALDSNPRFG 310 320 330 340 350 360 DKKTYQMNPA NYREALTEMR EDESEGADIL LVKPGLPYLD IIRLLRDNSP LPIAAYQVSG 370 380 390 400 410 EYAMIKAAGA LKMIDEEKVM MESLMCLRRA GADIILTYSA LQAARCLCGE KR

References

[1]

"Plant delta-aminolevulinic acid dehydratase. Expression in soybean root nodules and evidence for a bacterial lineage of the Alad gene." Kaczor C.M., Smith M.W., Sangwan I., O'Brian M.R. Plant Physiol. 104:1411-1417(1994) [PubMed: 8016269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U04525 mRNA. Translation: AAA18342.1.
PIR	T06351.
UniGene	Gma.6038
3D structure databases
HSSP	HSSP built from PDB template 1E51 based on UniProtKB P13716.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.2.1.24. 299.
Gene expression databases
Genevestigator	P43210.
Family and domain databases
InterPro	IPR001731. 4pyrrol_synth_porphobiln_synth. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PRINTS	PR00144. DALDHYDRTASE.
ProDom	PD002304. AlaD_dehydratase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM2_SOYBN

Accession

Primary (citable) accession number: P43210

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	October 13, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)