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P43210

- HEM2_SOYBN

UniProt

P43210 - HEM2_SOYBN

Protein

Delta-aminolevulinic acid dehydratase, chloroplastic

Gene

HEMB

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Is committed to plant tetrapyrrole synthesis.
    Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.By similarity

    Catalytic activityi

    2 5-aminolevulinate = porphobilinogen + 2 H2O.

    Cofactori

    Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as allosteric activator By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei280 – 2801Schiff-base intermediate with substrateBy similarity
    Binding sitei290 – 2901Substrate 1By similarity
    Binding sitei302 – 3021Substrate 1By similarity
    Metal bindingi318 – 3181MagnesiumBy similarity
    Active sitei333 – 3331Schiff-base intermediate with substrateBy similarity
    Binding sitei359 – 3591Substrate 2By similarity
    Binding sitei398 – 3981Substrate 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. porphobilinogen synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. chlorophyll biosynthetic process Source: UniProtKB-KW
    2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00251; UER00318.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-aminolevulinic acid dehydratase, chloroplastic (EC:4.2.1.24)
    Short name:
    ALADH
    Alternative name(s):
    Porphobilinogen synthase
    Gene namesi
    Name:HEMB
    Synonyms:ALAD
    OrganismiGlycine max (Soybean) (Glycine hispida)
    Taxonomic identifieri3847 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
    ProteomesiUP000008827: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4848ChloroplastSequence AnalysisAdd
    BLAST
    Chaini49 – 412364Delta-aminolevulinic acid dehydratase, chloroplasticPRO_0000013320Add
    BLAST

    Proteomic databases

    ProMEXiP43210.

    Expressioni

    Tissue specificityi

    Leaves and root nodules.

    Gene expression databases

    GenevestigatoriP43210.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP43210.
    SMRiP43210. Positions 88-407.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALADH family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK01698.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view]
    PANTHERiPTHR11458. PTHR11458. 1 hit.
    PfamiPF00490. ALAD. 1 hit.
    [Graphical view]
    PRINTSiPR00144. DALDHYDRTASE.
    SMARTiSM01004. ALAD. 1 hit.
    [Graphical view]
    PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43210-1 [UniParc]FASTAAdd to Basket

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    MASSIPNAPS AFNSQSYVGL RAPLRTFNFS SPQAAKIPRS QRLFVVRASD    50
    SEFEAAVVAG KVPPAPPVRP RPAAPVGTPV VPSLPLHRRP RRNRKSPALR 100
    SAFQETSISP ANFVYPLFIH EGEEDTPIGA MPGCYRLGWR HGLVEEVAKA 150
    RDVGVNSVVL FPKIPDALKS PTGDEAYNEN GLVPRTIRLL KDKYPDLVIY 200
    TDVALDPYSS DGHDGIVRED GVIMNDETVH QLCKQAVAQA QAGADVVSPS 250
    DMMDGRVGAL RAALDAEGFQ HVSIMSYTAK YASSFYGPFR EALDSNPRFG 300
    DKKTYQMNPA NYREALTEMR EDESEGADIL LVKPGLPYLD IIRLLRDNSP 350
    LPIAAYQVSG EYAMIKAAGA LKMIDEEKVM MESLMCLRRA GADIILTYSA 400
    LQAARCLCGE KR 412
    Length:412
    Mass (Da):45,068
    Last modified:November 1, 1995 - v1
    Checksum:i0ECDF209A09572D8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04525 mRNA. Translation: AAA18342.1.
    PIRiT06351.
    RefSeqiNP_001238737.1. NM_001251808.1.
    UniGeneiGma.6038.

    Genome annotation databases

    GeneIDi548095.
    KEGGigmx:548095.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04525 mRNA. Translation: AAA18342.1 .
    PIRi T06351.
    RefSeqi NP_001238737.1. NM_001251808.1.
    UniGenei Gma.6038.

    3D structure databases

    ProteinModelPortali P43210.
    SMRi P43210. Positions 88-407.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    ProMEXi P43210.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 548095.
    KEGGi gmx:548095.

    Phylogenomic databases

    KOi K01698.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00318 .

    Gene expression databases

    Genevestigatori P43210.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view ]
    PANTHERi PTHR11458. PTHR11458. 1 hit.
    Pfami PF00490. ALAD. 1 hit.
    [Graphical view ]
    PRINTSi PR00144. DALDHYDRTASE.
    SMARTi SM01004. ALAD. 1 hit.
    [Graphical view ]
    PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Plant delta-aminolevulinic acid dehydratase. Expression in soybean root nodules and evidence for a bacterial lineage of the Alad gene."
      Kaczor C.M., Smith M.W., Sangwan I., O'Brian M.R.
      Plant Physiol. 104:1411-1417(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Leaf.

    Entry informationi

    Entry nameiHEM2_SOYBN
    AccessioniPrimary (citable) accession number: P43210
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3