P43210 (HEM2_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Delta-aminolevulinic acid dehydratase, chloroplastic
Short name=ALADH
EC=4.2.1.24

Alternative name(s):
Porphobilinogen synthase

Gene names

Name:	HEMB
Synonyms:	ALAD

Organism

Glycine max (Soybean) (Glycine hispida) [Reference proteome]

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Is committed to plant tetrapyrrole synthesis. Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.
Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Cofactor	Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as allosteric activator By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Subcellular location	Plastid › chloroplast.
Tissue specificity	Leaves and root nodules.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Heme biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 48	48	Chloroplast Potential
	Chain	49 – 412	364	Delta-aminolevulinic acid dehydratase, chloroplastic		PRO_0000013320
Sites
	Active site	280	1	Schiff-base intermediate with substrate By similarity
	Active site	333	1	Schiff-base intermediate with substrate By similarity
	Metal binding	318	1	Magnesium By similarity
	Binding site	290	1	Substrate 1 By similarity
	Binding site	302	1	Substrate 1 By similarity
	Binding site	359	1	Substrate 2 By similarity
	Binding site	398	1	Substrate 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43210 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 0ECDF209A09572D8	FASTA	412	45,068
	10 20 30 40 50 60 MASSIPNAPS AFNSQSYVGL RAPLRTFNFS SPQAAKIPRS QRLFVVRASD SEFEAAVVAG 70 80 90 100 110 120 KVPPAPPVRP RPAAPVGTPV VPSLPLHRRP RRNRKSPALR SAFQETSISP ANFVYPLFIH 130 140 150 160 170 180 EGEEDTPIGA MPGCYRLGWR HGLVEEVAKA RDVGVNSVVL FPKIPDALKS PTGDEAYNEN 190 200 210 220 230 240 GLVPRTIRLL KDKYPDLVIY TDVALDPYSS DGHDGIVRED GVIMNDETVH QLCKQAVAQA 250 260 270 280 290 300 QAGADVVSPS DMMDGRVGAL RAALDAEGFQ HVSIMSYTAK YASSFYGPFR EALDSNPRFG 310 320 330 340 350 360 DKKTYQMNPA NYREALTEMR EDESEGADIL LVKPGLPYLD IIRLLRDNSP LPIAAYQVSG 370 380 390 400 410 EYAMIKAAGA LKMIDEEKVM MESLMCLRRA GADIILTYSA LQAARCLCGE KR

References

[1]

"Plant delta-aminolevulinic acid dehydratase. Expression in soybean root nodules and evidence for a bacterial lineage of the Alad gene." Kaczor C.M., Smith M.W., Sangwan I., O'Brian M.R. Plant Physiol. 104:1411-1417(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U04525 mRNA. Translation: AAA18342.1.
PIR	T06351.
RefSeq	NP_001238737.1. NM_001251808.1.
UniGene	Gma.6038.
3D structure databases
ProteinModelPortal	P43210.
SMR	P43210. Positions 88-407.
ModBase	Search...
Proteomic databases
ProMEX	P43210.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	548095.
KEGG	gmx:548095.
Phylogenomic databases
KO	K01698.
Enzyme and pathway databases
UniPathway	UPA00251; UER00318.
Gene expression databases
Genevestigator	P43210.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR001731. Porphobilinogen_synth. [Graphical view]
PANTHER	PTHR11458. PTHR11458. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PRINTS	PR00144. DALDHYDRTASE.
SMART	SM01004. ALAD. 1 hit. [Graphical view]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM2_SOYBN

Accession

Primary (citable) accession number: P43210

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program